阅读说明：本技术 一种玫瑰扦插繁育方法 (Rose cuttage breeding method ) 是由 景珑 武光 吕会甫 于 2021-07-02 设计创作，主要内容包括：本申请涉及玫瑰繁育的领域,具体公开了一种玫瑰扦插繁育方法,其步骤包括选取插穗、苗床处理、扦插处理、扦插管理,扦插管理过程中插穗扦插后每隔8-10h喷涂生根液,每隔2-3h进行一次浇水,每隔3-5h喷涂营养液；扦插7-9天后每天上午8点至11点将大棚上的遮光网去除；所述营养液的制备原料按重量份计包括5-12份恶霉灵、3-10份印楝素、10-15份腐殖酸、0.5-2份聚醚改性有机硅表面活性剂、0.05-0.8份光合作用促进剂、8-17份乙醇、3-7份硅酸镁铝、5-15份羟乙基纤维素、0.5-1份壳聚糖；本申请的扦插繁育方法具有提高玫瑰成活率及抗病虫害性能的优点。(The application relates to the field of rose breeding, and particularly discloses a rose cutting breeding method which comprises the steps of selecting cutting slips, seedbed processing, cutting processing and cutting management, wherein in the cutting management process, rooting liquid is sprayed every 8-10h after the cutting slips are cut, watering is carried out every 2-3h, and nutrient solution is sprayed every 3-5 h; removing the shading net on the greenhouse from 8 to 11 am every day after 7 to 9 days of cuttage; the raw materials for preparing the nutrient solution comprise, by weight, 5-12 parts of hymexazol, 3-10 parts of azadirachtin, 10-15 parts of humic acid, 0.5-2 parts of polyether modified organic silicon surfactant, 0.05-0.8 part of photosynthesis promoter, 8-17 parts of ethanol, 3-7 parts of magnesium aluminum silicate, 5-15 parts of hydroxyethyl cellulose and 0.5-1 part of chitosan; the cuttage breeding method has the advantages of improving the survival rate of the roses and the disease and pest resistance.)

1. A rose cutting propagation method is characterized by comprising the following steps:

selecting cutting shoots: selecting current-year young shoots with flowers as cutting slips, wherein the transverse diameter of each cutting slip is 0.8-2cm, the length of each cutting slip is 8-12cm, the cutting slip is provided with at least 2 nodes, 2-3 small leaves are left at the top end, and the lower ends of the cutting slips are cut into horseshoe-shaped inclined planes for later use;

seedbed treatment: building a greenhouse by a conventional method, externally arranging a shading net with shading rate of 60-70%, paving a cuttage bed in the greenhouse, placing a hole tray on the cuttage bed, and paving cuttage soil with thickness of 3-5cm at the bottom of the hole tray;

cutting treatment: sterilizing the cutting shoots within 10-20 minutes after the cutting shoots are obtained, soaking the cutting shoots in a rooting solution, inserting the cutting shoots into a plug tray, backfilling cutting soil, and watering thoroughly, wherein the thickness of the soil in the plug tray is 5-7cm after the cutting soil is backfilled;

cuttage management: keeping the temperature in the greenhouse at 15-25 ℃, keeping the relative humidity of air at 70-80%, spraying rooting liquid every 8-10h after each cutting shoot is subjected to cuttage, watering every 2-3h, and spraying nutrient solution every 3-5 h; removing the shading net on the greenhouse from 8 to 11 am every day after 7 to 9 days of cuttage;

the raw materials for preparing the nutrient solution comprise, by weight, 5-12 parts of hymexazol, 3-10 parts of azadirachtin, 10-15 parts of humic acid, 0.5-2 parts of polyether modified organic silicon surfactant, 0.05-0.8 part of photosynthesis promoter, 8-17 parts of ethanol, 3-7 parts of magnesium aluminum silicate, 5-15 parts of hydroxyethyl cellulose and 0.5-1 part of chitosan.

2. The rose cutting propagation method according to claim 1, characterized in that: the preparation raw materials of the cutting soil comprise, by weight, 10: (1-2): (0.5-1.5): (0.3-0.7): (2-4) leaf mold, coconut coir, ginger powder, super absorbent resin and perlite.

3. The rose cutting propagation method according to claim 1, characterized in that: the rooting solution comprises the following components in percentage by weight of 3: (0.08-0.1): (0.002-0.005): (5-15) mixing the ABT rooting powder, L-aspartic acid, brassinolide and water.

4. The rose cutting propagation method according to claim 3, characterized in that: the rooting solution comprises the following components in percentage by weight of 3: (0.08-0.1): 0.004: 12, L-aspartic acid, brassinolide and water.

5. The rose cutting propagation method according to claim 1, characterized in that: and in the cuttage management step, 5-15g of rooting liquid is sprayed on each cutting shoot.

6. The rose cutting propagation method according to claim 1, characterized in that: and in the cuttage management step, 3-7g of nutrient solution is sprayed on each cutting shoot each time.

7. The rose cutting propagation method according to claim 1, characterized in that: and in the cuttage management step, nutrient solution is sprayed 3-7 days after the cutting.

8. The rose cutting propagation method according to claim 1, characterized in that: the chitosan is a mixture of carboxymethyl chitosan and hydroxyethyl chitosan.

9. The rose cutting propagation method according to claim 8, characterized in that: the weight ratio of the carboxymethyl chitosan to the hydroxyethyl chitosan is (1-3) to 2.

Technical Field

The application relates to the field of rose breeding, in particular to a rose cuttage breeding method.

Background

Roses are called "the king of flowers" in China, and are one of the ten famous flowers in the world. The rose has high ornamental value, medicinal value and nutritive value, is used for expressing beauty and love for a long time and has extremely wide application.

The roses are more in variety, including purple branch roses, white roses, Damascus roses and the like, the current breeding of the roses mostly adopts a sowing or cutting method, compared with sowing, the cutting method is easy in material taking, simple in operation and high in breeding speed, and therefore the rose cutting method is widely applied to modern rose breeding. However, the current rose cutting method has the problem of low survival rate, and the phenomenon of weak pest and disease resistance often occurs in the later planting after survival.

With respect to the above-described related art, the inventors consider that: the survival rate of rose cutting is improved, and meanwhile, the pest and disease damage rate of roses is reduced.

Disclosure of Invention

In order to improve the survival rate of rose cuttage and reduce the pest and disease damage rate of roses, the application provides a rose cuttage breeding method.

The rose cuttage breeding method provided by the application adopts the following technical scheme:

a rose cutting propagation method comprises the following steps:

selecting cutting shoots: selecting current-year young shoots with flowers as cutting slips, wherein the transverse diameter of each cutting slip is 0.8-2cm, the length of each cutting slip is 8-12cm, the cutting slip is provided with at least 2 nodes, 2-3 small leaves are left at the top end, and the lower ends of the cutting slips are cut into horseshoe-shaped inclined planes for later use;

seedbed treatment: building a greenhouse by a conventional method, externally arranging a shading net with shading rate of 60-70%, paving a cuttage bed in the greenhouse, placing a hole tray on the cuttage bed, and paving cuttage soil with thickness of 3-5cm at the bottom of the hole tray;

cutting treatment: sterilizing the cutting shoots within 10-20 minutes after the cutting shoots are obtained, soaking the cutting shoots in a rooting solution, inserting the cutting shoots into a plug tray, backfilling cutting soil, and watering thoroughly, wherein the thickness of the soil in the plug tray is 5-7cm after the cutting soil is backfilled;

cuttage management: keeping the temperature in the greenhouse at 15-25 ℃, keeping the relative humidity of air at 70-80%, spraying rooting liquid every 8-10h after cutting the cutting slips, watering every 2-3h, and spraying nutrient solution every 3-5 h; removing the shading net on the greenhouse from 8 to 11 am every day after 7 to 9 days of cuttage;

the raw materials for preparing the nutrient solution comprise, by weight, 5-12 parts of hymexazol, 3-10 parts of azadirachtin, 10-15 parts of humic acid, 0.5-2 parts of polyether modified organic silicon surfactant, 0.05-0.8 part of photosynthesis promoter, 8-17 parts of ethanol, 3-7 parts of magnesium aluminum silicate, 5-15 parts of hydroxyethyl cellulose and 0.5-1 part of chitosan.

By adopting the technical scheme, proper cutting slips and rose cutting are prepared and sprayed with the rooting solution and the nutrient solution, so that the survival rate and the pest and disease resistance of the rose are improved, the polyether modified organic silicon surfactant can promote the absorption of the rose on pesticides and nutrients, the magnesium aluminum silicate has higher water absorbability, cohesiveness and dispersibility due to the nanostructure, the hydroxyethyl cellulose is nonionic soluble cellulose ether, has good dispersibility and cohesiveness, and can act synergistically with magnesium aluminum silicate, so that the adhesiveness of the nutrient solution on rose leaves is improved, and the pest and disease resistance of the rose is improved.

Preferably, the preparation raw materials of the cutting soil comprise, by weight, 10: (1-2): (0.5-1.5): (0.3-0.7): (2-4) leaf mold, coconut coir, ginger powder, super absorbent resin and perlite.

By adopting the technical scheme, the weight ratio of the raw materials is 10: (1-2): (0.5-1.5): (0.3-0.7): and (2) the cutting soil of the leaf mold, the coconut coir, the ginger powder, the super absorbent resin and the perlite is high in nutrition, and the rose cutting is beneficial to breeding of the rose cutting.

Preferably, the rooting solution comprises the following components in percentage by weight of 3: (0.08-0.1): (0.002-0.005): (5-15) mixing the ABT rooting powder, L-aspartic acid, brassinolide and water.

By adopting the technical scheme, the L-aspartic acid and the brassinolide are added into the rooting solution and interact with each other, so that the disease and insect pest resistance and the survival rate of the roses are improved.

Preferably, the rooting solution comprises the following components in percentage by weight of 3: (0.08-0.1): 0.004: 12, L-aspartic acid, brassinolide and water.

By adopting the technical scheme, when the weight ratio of ABT rooting powder, indoleacetic acid, L-aspartic acid, brassinolide and water is 3: (0.08-0.1): 0.004: when 12 hours, the survival rate and the disease and insect resistance of the roses are higher.

Preferably, in the cuttage management step, 5-15g of rooting liquid is sprayed on each cutting shoot.

Preferably, in the cuttage management step, 3-7g of nutrient solution is sprayed on each cutting shoot.

Preferably, in the step of managing cuttage, nutrient solution is sprayed 3-7 days after the cutting.

By adopting the technical scheme, the nutrient solution is sprayed after the cutting shoots grow for 3-7, the cutting shoots can better absorb the nutrient solution, and the survival rate and the disease and insect resistance of the bred roses are higher.

Preferably, the chitosan is a mixture of carboxymethyl chitosan and hydroxyethyl chitosan.

By adopting the technical scheme, the compatibility of the carboxymethyl chitosan and the hydroxyethyl chitosan in a nutrient solution system is higher, and the carboxymethyl chitosan and the hydroxyethyl chitosan can interact with each other, so that the survival rate and the disease and insect resistance of the bred rose are improved.

Preferably, the weight ratio of the carboxymethyl chitosan to the hydroxyethyl chitosan is (1-3): 2.

By adopting the technical scheme, the survival rate and the disease and insect resistance of the bred roses are further improved by controlling the weight ratio of the carboxymethyl chitosan to the hydroxyethyl chitosan to be (1-3): 2.

In summary, the present application has the following beneficial effects:

1. according to the method, after the rose is properly cut, the rose is properly cut and then the rooting solution and the nutrient solution are sprayed, the polyether modified organic silicon surfactant in the nutrient solution can promote the absorption of the rose on pesticides and nutrients, the magnesium aluminum silicate has higher water absorbability, cohesiveness and dispersibility due to the nanostructure, the hydroxyethyl cellulose is nonionic soluble cellulose ether, has good dispersibility and cohesiveness, and can act in cooperation with magnesium aluminum silicate, so that the adhesiveness of the nutrient solution on the rose leaves is improved, and the disease and insect resistance of the rose is improved;

2. in the application, the nutrient solution is preferably sprayed 3-7 days after the cutting, at the moment, the cutting absorbs the nutrient solution better, and the survival rate and the disease and insect resistance of the bred roses are higher;

3. in the application, the chitosan in the nutrient solution is preferably a mixture of carboxymethyl chitosan and hydroxyethyl chitosan, and the carboxymethyl chitosan and the hydroxyethyl chitosan can interact with each other, so that the survival rate and the disease and insect resistance of the bred roses are improved.

Detailed Description

The present application will be described in further detail with reference to the following preparation examples and examples, wherein Damascus roses used in the present application are two-year-old roses purchased from Zhenxin county, Rose town, Xinye rose nursery; the purple branch rose is a two-year rose purchased from the professional cooperative society of the Spanish Marble flower seedlings in Qingzhou city; the remaining sources of raw materials used in this application are shown in Table 1 unless otherwise specified.

TABLE 1 sources of raw materials used in the present application

Preparation example of nutrient solution

Preparation example 1

A nutrient solution is prepared by the following steps: uniformly mixing 8kg of hymexazol, 5kg of azadirachtin, 13kg of humic acid, 1.5kg of polyether modified organic silicon surfactant, 0.3kg of photosynthesis promoter, 12kg of absolute ethyl alcohol, 5kg of magnesium aluminum silicate, 10kg of hydroxyethyl cellulose and 0.7kg of chitosan to prepare a nutrient solution;

the chitosan is carboxymethyl chitosan.

Preparation examples 2 to 7

Preparation examples 2 to 7 are based on preparation example 1 and differ from preparation example 1 only in that: the amounts of the raw materials are different, and are shown in Table 2.

TABLE 2 preparation examples 1 to 7 respective amounts of raw materials

Preparation example 8

Preparation 8 is based on preparation 1, differing from preparation 1 only in that: the chitosan is hydroxyethyl chitosan.

Preparation example 9

Preparation 9 is based on preparation 1, differing from preparation 1 only in that: the chitosan is a mixture of carboxymethyl chitosan and hydroxyethyl chitosan, and the weight ratio of the carboxymethyl chitosan to the hydroxyethyl chitosan is 0.5: 2.

Preparation example 10

Preparation 10 is based on preparation 9 and differs from preparation 9 only in that: the weight ratio of the carboxymethyl chitosan to the hydroxyethyl chitosan is 5: 2.

Preparation example 11

Preparation 11 is based on preparation 9, and differs from preparation 9 only in that: the weight ratio of the carboxymethyl chitosan to the hydroxyethyl chitosan is 1: 2.

Preparation example 12

Preparation 12 is based on preparation 9 and differs from preparation 9 only in that: the weight ratio of the carboxymethyl chitosan to the hydroxyethyl chitosan is 1: 1.

Preparation example 13

Preparation 13 is based on preparation 9 and differs from preparation 9 only in that: the weight ratio of the carboxymethyl chitosan to the hydroxyethyl chitosan is 3: 2.

Preparation example of cutting soil

Preparation example 14

A cutting soil is prepared by the following steps: uniformly mixing 10kg of leaf mold, 1.5kg of coconut coir, 1kg of ginger powder, 0.5kg of super absorbent resin and 3kg of perlite to obtain the cutting soil.

Preparation examples 15 to 16

Preparation examples 15 to 16 are based on preparation example 14 and differ from preparation example 14 only in that: the dosage of each raw material is different, and the specific table is shown in table 3.

TABLE 3 preparation examples 14 to 16 each raw material amount

Preparation example	Preparation example 14	Preparation example 15	Preparation example 16
				Leaf of rotten leaf soil (kg)	10	10	10
Coconut husk (kg)	1.5	1	2
				Ginger (fresh ginger)Powder (kg)	1.0	1.5	0.5
Super absorbent resin (kg)	0.5	0.7	0.3
				Perlite (kg)	3	4	2

Example of preparation of rooting solution

Preparation example 17

A rooting solution is prepared by the following steps: 3kg of ABT rooting powder, 0.095kg of L-aspartic acid, 0.003kg of brassinolide and 10kg of water are uniformly mixed to prepare rooting solution.

Preparation examples 18 to 21

Preparation examples 18 to 21 are based on preparation example 17, differing from preparation example 17 only in that: the amounts of the raw materials are different, and are shown in Table 4.

TABLE 4 preparation examples 17 to 21 amounts of the respective raw materials

Preparation example 22

Preparation 22 is based on preparation 17, and differs from preparation 17 only in that: the equal-quality rooting powder is used for replacing brassinolide.

Preparation example 23

Preparation 23 is based on preparation 17, and differs from preparation 17 only in that: equal mass of rooting powder is used to replace L-aspartic acid.

Comparative preparation example

Comparative preparation example 1

Comparative preparation example 1 is based on preparation example 1, differing from preparation example 1 only in that: hydroxypropyl cellulose of equal mass is used to replace hydroxyethyl cellulose, the type of the used hydroxypropyl cellulose is FQ-6000, and the hydroxypropyl cellulose is purchased from novel building material Co.

Comparative preparation example 2

Comparative example preparation example 2 is based on preparation example 1, differing from preparation example 1 only in that: the magnesium aluminum silicate is replaced by hydroxyethyl cellulose with equal mass.

Examples

Example 1

A rose cutting propagation method comprises the following steps:

selecting cutting shoots: selecting current-year young shoots with bloomed flowers as cutting slips, wherein the transverse diameter of each cutting slip is 1.5cm, the length of each cutting slip is 10cm, the cutting slip is provided with at least 2 sections, and 3 small leaves are left at the top end of each cutting slip, and the lower ends of the cutting slips are cut into horseshoe-shaped inclined planes for later use;

seedbed treatment: building a greenhouse by a conventional method, externally arranging a shading net with shading rate of 65%, laying a cuttage bed in the greenhouse, placing a hole tray on the cuttage bed, and laying cuttage soil with thickness of 4cm at the bottom of the hole tray;

cutting treatment: disinfecting the cutting slips by using a potassium permanganate solution with the concentration of 10% within 15 minutes after the cutting slips are obtained, soaking the cutting slips in a rooting solution for 10 minutes, inserting the cutting slips into a plug tray, backfilling cutting soil, enabling the thickness of the soil in the plug tray to be 5cm after the cutting soil is backfilled, and then watering thoroughly;

cuttage management: keeping the temperature in the greenhouse at 20 ℃, keeping the relative air humidity at 70%, spraying a rooting solution every 9h after each cutting shoot is subjected to cuttage, spraying 12g of the rooting solution every time for each cutting shoot, watering every 2h, watering 500mL every time for each cutting shoot, spraying a nutrient solution every 4h, spraying 5g of the nutrient solution every time for each cutting shoot, and removing a shading net on the greenhouse from 8 am to 11 am every 8 days after cuttage for 8 days;

the nutrient solution used was derived from preparation example 1; the cutting soil is prepared by uniformly mixing 10kg of leaf mold, 1.5kg of coconut coir, 1kg of ginger powder and 3kg of perlite; the rooting solution is live forest hairy root purchased from Zhengzhou Senya agriculture science and technology limited.

Examples 2 to 3

Examples 2 to 3 are based on example 1 and differ from example 1 only in that: the cutting conditions are different, and are shown in table 5.

TABLE 5 examples 1-3 cuttage conditions

Examples 4 to 6

Examples 4 to 6 are based on example 1 and differ from example 1 only in that: the sources of the cutting soil used are different, and are shown in table 6.

TABLE 6 examples 4-6 sources of cutting soil

Examples	Example 4	Example 5	Example 6
				Source of cutting soil	Preparation example 14	Preparation example 15	Preparation example 16

Examples 7 to 13

Examples 7 to 13 are based on example 4 and differ from example 4 only in that: the sources of the rooting liquid used are different and are shown in table 7.

TABLE 7 example 7-13 rooting liquid sources

Examples	Source of rooting liquid
		Example 7	Preparation example 17
Example 8	Preparation example 18
		Example 9	Preparation example 19
Example 10	Preparation example 20
		Example 11	Preparation example 21
Example 12	Preparation example 22
		Example 13	Preparation example 23

Examples 14 to 25

Examples 14 to 25 are based on example 11 and differ from example 11 only in that: the source of the nutrient solution used was varied and is shown in Table 8.

TABLE 8 sources of nutrient solutions of examples 14-25

Example 26

Example 26 is based on example 25 and differs from example 25 only in that: and in the cuttage management step, nutrient solution is sprayed 3 days after the cutting.

Example 27

Example 27 is based on example 25 and differs from example 25 only in that: and in the cuttage management step, nutrient solution spraying is started 7 days after the cutting.

Example 28

Example 28 is based on example 25 and differs from example 25 only in that: and in the cuttage management step, nutrient solution is sprayed 5 days after the cutting.

Comparative example

Comparative example 1

Comparative example 1 is based on example 1 and differs from example 1 only in that: the nutrient solution used was from comparative preparation example 1.

Comparative example 2

Comparative example 2 is based on example 1 and differs from example 1 only in that: the nutrient solution used was from comparative preparation example 2.

Comparative example 3

Comparative example 3 is based on example 1 and differs from example 1 only in that: nutrient solution is not sprayed in the cutting management step.

Performance test

Experiment group one: selecting two-year-old rosa damascena to perform cuttage according to the methods of examples 1-28 and comparative examples 1-3, performing cuttage with 400 cuttings in each example/comparative example, and testing the survival rate and the pest and disease damage rate of the rosa damascena after cuttage breeding, wherein the pest and disease damage rate is the number of cuttage roses with pests/the total number of cuttage roses multiplied by 100%, and the test results are shown in table 9.

Experiment group two: selecting two-year-old purple branch roses to perform cuttage according to the methods of examples 1-28 and comparative examples 1-3, performing cuttage on 400 cuttings in each example/comparative example, and testing the survival rate and the pest and disease damage rate of the purple branch roses after cuttage breeding, wherein the pest and disease damage rate is the number of cuttage roses with pests/the total number of the cuttage roses multiplied by 100%, and the test results are shown in table 9.

TABLE 9 test results of examples 1-28 and comparative examples 1-3

The data are analyzed, so that the cutting propagation method can not only improve the survival rate of the roses, but also reduce the pest and disease damage rate of the roses and improve the pest and disease resistance of the roses, has wide application range, is suitable for cutting propagation of various roses such as rosa damascena and rosa purpurea, and the data of the embodiments 1 to 3 are analyzed, so that the embodiment 1 is the best embodiment of the embodiments 1 to 3.

Analyzing the data of the example 1 and the comparative examples 1 to 3, the cuttage breeding method disclosed by the application is used for cultivating roses, and the specific nutrient solution is added in the cuttage breeding process, so that the survival rate of the roses can be improved, the pest and disease damage rate of the roses can be reduced, namely, the pest and disease resistance of the roses is improved, the polyether modified organic silicon surfactant can promote the absorption of the roses on pesticides and nutrients, the magnesium aluminum silicate has higher water absorption, caking property and dispersibility due to the nanometer structure of the magnesium aluminum silicate, the hydroxyethyl cellulose is nonionic soluble cellulose ether, has good dispersibility and caking property, and can act with the magnesium aluminum silicate synergistically, and therefore, the survival rate and the pest and disease resistance of the roses are improved.

Analyzing the data of examples 4-6 and example 1, it can be seen that when the weight ratio of 10: (1-2): (0.5-1.5): (0.3-0.7): and (2) when the cutting soil prepared from the leaf mold, the coconut coir, the ginger powder, the super absorbent resin and the perlite is used, the survival rate of the roses is improved, the pest and disease damage rate is reduced, and the disease and pest resistance of the roses is improved.

By analyzing the data of examples 7 to 9, 4 and 12 to 13, the weight ratio of 3: (0.08-0.1): (0.002-0.005): (5-15) when the rooting solution is prepared from ABT rooting powder, L-aspartic acid, brassinolide and water, the pest and disease damage rate of the roses is low, and the survival rate of the roses is high; the L-aspartic acid and the brassinolide in the rooting solution can interact with each other, so that the disease and insect resistance and the survival rate of the roses are improved.

Analyzing the data of the pairs of examples 10 to 11 and example 4, it can be seen that when the weight ratio of ABT rooting powder, L-aspartic acid, brassinolide and water in the controlled rooting solution is 3: (0.08-0.1): 0.004: when 12 hours, the survival rate and the disease and insect resistance of the roses are higher.

Analysis of the data of examples 21-22, 20 and 11 shows that when the chitosan used in the rooting solution is a mixture of carboxymethyl chitosan and hydroxyethyl chitosan, the pest and disease damage rate of roses is reduced, and the survival rate is improved, which indicates that the compatibility of carboxymethyl chitosan and hydroxyethyl chitosan in the nutrient solution system is higher, and the carboxymethyl chitosan and hydroxyethyl chitosan can interact with each other, and the survival rate and pest and disease resistance of the bred roses are improved. As is clear from the analysis of the data in examples 23 to 25 and examples 21 to 22, when the weight ratio of carboxymethyl chitosan to hydroxyethyl chitosan was controlled to (1-3):2, the survival rate and disease and pest resistance of roses were high.

Analysis of the data of examples 26-28 and example 25 shows that the cutting shoots after 3-7 of growth are sprayed with the nutrient solution, the cutting shoots absorb the nutrient solution better, and the rose obtained by breeding has high survival rate and disease and pest resistance.

The present embodiment is only for explaining the present application, and it is not limited to the present application, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present application.