阅读说明：本技术 一种含艾叶黄酮类提取物的农药组合物 (Pesticide composition containing folium artemisiae argyi flavonoid extract ) 是由 徐凤波 李娇 李庆山 张智蕊 张博云 张宇 于 2021-01-26 设计创作，主要内容包括：本发明公开了一种含艾叶黄酮类提取物的农药组合物及其用途,所述农药组合物包括A和B两种活性成分,其中活性成分A为艾叶黄酮类提取物,活性成分B选自宁南霉素、辛菌胺醋酸盐、春雷霉素、吡唑醚菌酯、波尔多液、苯醚甲环唑等现有杀菌杀病毒农药。农药组合物包括常用助剂、活性成分A和活性成分B,活性成分A和活性成分B的质量份数比为80∶1～1∶80,应用于多种作物由细菌、病毒、真菌引起的多种病害的防治。组合物中活性成分表现为增效或者延长持效期,具有减量增效、持效期长、对作物安全性高等特点。(The invention discloses a pesticide composition containing folium artemisiae argyi flavonoid extract and application thereof, wherein the pesticide composition comprises two active ingredients A and B, the active ingredient A is folium artemisiae argyi flavonoid extract, and the active ingredient B is selected from existing sterilization and virucidal pesticides such as ningnanmycin, Xinjunan acetate, kasugamycin, pyraclostrobin, Bordeaux mixture, difenoconazole and the like. The pesticide composition comprises a common auxiliary agent, an active ingredient A and an active ingredient B, wherein the mass part ratio of the active ingredient A to the active ingredient B is 80: 1-1: 80, and the pesticide composition is applied to prevention and treatment of various diseases of various crops caused by bacteria, viruses and fungi. The active ingredients in the composition show synergism or prolonged effective period, and have the characteristics of decrement synergism, long effective period, high safety to crops and the like.)

1. A pesticide composition for preventing and treating plant bacterial, viral and fungal diseases is characterized in that active ingredients of the pesticide composition comprise two active ingredients A and B.

2. The pesticide composition as claimed in claim 1, wherein the active ingredient A is a folium artemisiae argyi flavonoid extract, and the active ingredient B is selected from existing bactericidal and viricidal pesticides such as ningnanmycin, xinjuniper acetate, kasugamycin, pyraclostrobin, boldo liquid, difenoconazole and the like.

3. The pesticide composition as claimed in claim 2, wherein the folium artemisiae argyi flavonoid extract is prepared by folium artemisiae argyi extraction.

4. The folium artemisiae argyi flavonoid extract in the pesticide composition according to claims 1 to 3, which is characterized in that: the preparation method comprises the following steps:

1) pulverizing folium Artemisiae Argyi into moxa;

2) adding 15-30 times of 70% ethanol, heating and refluxing for 2-4 times, and obtaining filtrate after 1.5-3 hours each time;

3) after the filtrate is diluted, adding metal salt, adjusting the pH value to be alkaline for complexing, and collecting precipitate after the complexing is finished;

4) dissolving the precipitate in 30% ethanol, adjusting pH to acidity, and decomplexing. Removing solvent, dissolving with methanol, filtering, and drying the filtrate to obtain folium Artemisiae Argyi flavonoid extract.

5. The pesticidal composition according to claim 1, characterized in that: the mass part ratio of the active component A to the active component B is 80: 1-1: 80.

6. The pesticidal composition according to claim 1, characterized in that: the mass part ratio of the active component A to the active component B is 50: 1-1: 50.

7. The pesticidal composition according to claim 1, characterized in that: the mass part ratio of the active component A to the active component B is 30: 1-1: 30.

8. The pesticidal composition according to claim 1, characterized in that: the mass part ratio of the active component A to the active component B is 20: 1-1: 15.

9. The pesticidal composition according to claim 1, characterized in that: the weight of the active component is 0.1-90% of the total weight of the composition, and the balance is agriculturally acceptable pesticide auxiliary agent.

10. The pesticidal composition according to claim 1, characterized in that: the formulation of the composition is one of suspending agent, microemulsion, aqueous emulsion, water dispersible granule, aqueous solution, wettable powder and missible oil.

11. The pesticide composition and the pesticide preparation thereof as claimed in any one of claims 1 to 10 for preventing and treating plant bacterial, viral and fungal diseases.

Technical Field

The invention belongs to the field of synergistic pesticide compositions, and particularly relates to a composition containing folium artemisiae argyi flavonoid extract and a bactericidal and viricidal pesticide.

Background

Chemical pesticides play a great role in agricultural production, but with the use of a large amount of chemical pesticides, diseases, pests and weeds begin to have resistance, so that the environment is polluted, and new pesticides with low toxicity, high efficiency and environmental protection are needed. The plant extract is from plants, is green, environment-friendly and degradable, and is environment-friendly; the contained substances are special, and pests are not easy to generate resistance, so that the pesticide has the potential of becoming a novel pesticide. The botanical pesticide is used alone, has slow effect and poor quick-acting property, and can improve the control effect by being compounded with chemical pesticide.

Plants with bacteriostatic and bactericidal activity are numerous and contain many developed Chinese herbal medicines. The folium artemisiae argyi has been used for preventing pestilence for thousands of years, and pharmacological research of modern medicine shows that the folium artemisiae argyi is a broad-spectrum antibacterial and antiviral drug and has a certain inhibition effect on viruses and bacteria. Folium artemisiae argyi and an extract thereof are widely applied to the medicine and health care industry and are hardly applied to the agricultural field. Scientific research shows that the main active ingredient of the folium artemisiae argyi is a flavonoid compound, so that the flavonoid compound in the folium artemisiae argyi is extracted and used as the main active ingredient of the botanical pesticide. Compounding the flavonoids extract of the folium artemisiae argyi with the existing sterilization and virus killing pesticide to obtain the novel pesticide composition.

Disclosure of Invention

The invention aims to provide a pesticide composition containing folium artemisiae argyi flavonoid extract and a bactericidal and viricidal pesticide, which is used for preventing and treating various plant diseases caused by bacteria, viruses and fungi.

The folium artemisiae argyi flavonoid extract contained in the pesticide composition is prepared by extracting folium artemisiae argyi, and the preparation method comprises the following steps:

1) pulverizing folium Artemisiae Argyi into moxa;

2) adding 15-30 times of 70% ethanol, heating and refluxing for 2-4 times, and obtaining filtrate after 1.5-3 hours each time;

3) after the filtrate is diluted, adding metal salt, adjusting the pH value to be alkaline for complexing, and collecting precipitate after the complexing is finished;

4) dissolving the precipitate in 30% ethanol, adjusting pH to acidity, and decomplexing. Removing solvent, dissolving with methanol, filtering, and drying the filtrate to obtain folium Artemisiae Argyi flavonoid extract.

The pesticide composition is realized by the following technical scheme:

the pesticide composition containing the folium artemisiae argyi flavonoid extract and the bactericidal and viricidal pesticide has two active ingredients, namely an active ingredient A and an active ingredient B, wherein the active ingredient A is the folium artemisiae argyi flavonoid extract, and the active ingredient B is selected from the existing bactericidal and viricidal pesticides such as ningnanmycin, xinjunopan acetate, kasugamycin, pyraclostrobin, boldo liquid, difenoconazole and the like. The mass part ratio of the active component A to the active component B is 80: 1-1: 80, the preferable ratio is 50: 1-1: 50, the further preferable ratio is 30: 1-1: 30, and the further preferable ratio is 20: 1-1: 15.

The pesticide composition containing the folium artemisiae argyi flavonoid extract and the bactericidal and viricidal pesticide disclosed by the invention contains an active ingredient folium artemisiae argyi flavonoid extract and the bactericidal and viricidal pesticide, and also contains a pesticide auxiliary agent; the weight of the active component is 0.1-90% of the total weight of the composition, and the balance is agriculturally acceptable pesticide auxiliary agent.

The pesticide composition can be prepared into various formulations acceptable in pesticides, and the formulations of the pesticide composition adopt suspending agents, micro-emulsions, aqueous emulsions, water dispersible granules, aqueous solutions, wettable powder and missible oil.

The pesticide adjuvant can specifically comprise one or more of wetting agent, dispersing agent, antifreezing agent, emulsifier, penetrating agent, thickening agent, preservative, stabilizer, defoaming agent, disintegrating agent, filler and organic solvent, and the specific components and the dosage are determined by tests.

The wetting agent can be one or more selected from sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, sodium butyl naphthalene sulfonate, sodium alkyl sulfonate, sodium triisopropyl naphthalene sulfonate, sodium diisobutyl naphthalene sulfonate, dioctyl sodium sulfosuccinate, fatty alcohol polyoxyethylene ether sulfate and alkylphenol polyoxyethylene ether sulfosuccinate.

The dispersant can be one or more selected from alkylphenol polyoxyethylene ether formaldehyde condensate, naphthalene formaldehyde condensate sulfonate, benzene sulfonate, alkyl naphthalene formaldehyde condensate sulfonate, polycarboxylate, alkylphenol ethoxylates, fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether sulfonate, lignin and its derivative sulfonate and phosphate.

The antifreeze can be one or more selected from ethylene glycol, propylene glycol, diethylene glycol, isopropanol, glycerol and urea.

The emulsifier can be one or more selected from alkylphenol polyoxyethylene ether, benzyl phenol polyoxyethylene ether, phenethylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether and similar products, phenethylphenol polyoxyethylene ether, polyoxypropylene ether and similar products, fatty amine polyoxyethylene ether, fatty acid ethylene oxide addition product, castor oil ethylene oxide addition product and derivatives thereof, polyol fatty acid ester and ethylene oxide addition product thereof, sulfonate, sulfate, phosphate, phosphite, carboxylate (fatty carboxylate), alkylphenol polyoxyethylene ether formaldehyde condensate, polyoxyethylene polyoxypropylene block copolymer and alkylphenol polyoxyethylene ether formaldehyde condensate sulfate.

The penetrating agent can be one or more selected from siloxane polyoxyethylene ether, fatty alcohol, alcohol ether succinate, phenol ether succinate and alcohol ether phosphate.

The thickener is one or more selected from xanthan gum, arabic gum, magnesium aluminum silicate, methylcellulose, sodium carboxymethylcellulose, hydroxypropyl cellulose, sodium acrylate, polyvinyl alcohol, sodium polyacrylate, fumed silica, and organic bentonite.

The antiseptic can be one or more selected from sodium benzoate, sorbic acid, formaldehyde, phenyl salicylate, butyl p-hydroxybenzoate, potassium sorbate, sodium salicylate, and KASONG.

The stabilizer may be one or more selected from 2, 6-di-tert-butyl-4-methylphenol (BHT), citric acid and Butyl Hydroxy Anisol (BHA).

The defoaming agent can be one or more selected from C8-10 fatty alcohol, C19-20 saturated aliphatic carboxylic acid, epoxidized soybean oil, methanol, silicone oil, polyether defoaming agent and organic silicone.

The disintegrating agent can be one or more selected from urea, glucose, starch, sodium carboxymethylcellulose, and ammonium sulfate.

The filler can be one or more selected from diatomite, attapulgite, bentonite, light calcium carbonate, sepiolite, pottery clay, kaolin, white carbon black, quartz sand, talcum powder, clay, lignin, montmorillonite, corn starch, ammonium sulfate, and wood flour.

The organic solvent can be one or more selected from alcohol, aromatic hydrocarbon, ketone, ether, fatty acid ester, aliphatic hydrocarbon and vegetable oil.

The pesticide composition can be used for preventing and treating plant bacterial, viral and fungal diseases, particularly citrus anthracnose, peach gummosis, citrus scab, kiwifruit canker, cucumber powdery mildew and banana leaf spot, but the specific diseases of the pesticide composition can be prevented and treated in addition to the diseases, including but not limited to cucumbers (such as bacterial angular leaf spot, rhizoctonia rot and leaf blight), watermelons (such as bacterial angular leaf spot), tomatoes (such as bacterial wilt, ulcer, scab and bacterial leaf spot), peppers (such as bacterial wilt, scab, soft rot and bacterial leaf spot), Chinese cabbages (such as cabbage soft rot, black rot, Chinese cabbage angular leaf spot and leaf spot), cabbages (such as cabbage soft rot, black rot and bacterial black spot), potatoes (such as bacterial wilt, black rot, bacterial black spot and potato leaf spot) Ring rot, soft rot), kidney beans (e.g.: bacterial blight, blight), eggplant (e.g.: bacterial wilt), peach (e.g.: bacterial perforation disease).

According to the invention, the folium artemisiae argyi flavonoid extract and the sterilization and virucidal pesticide are compounded to obtain the novel pesticide composition, and the novel pesticide composition is applied to prevention and treatment of various diseases caused by bacteria, viruses and fungi after being compounded, so that the prevention and treatment range of the pesticide on plant diseases is remarkably expanded, the pesticide dosage and times are reduced, the pesticide duration is prolonged, and the use cost is reduced. The pesticide composition contains various bioactive components, pathogenic bacteria are not easy to generate drug resistance, and the drug resistance of the pathogenic bacteria to a single preparation can be delayed.

Detailed Description

The following description is given with reference to specific embodiments, which are only exemplary and do not limit the scope of the present invention in any way. Any obvious modifications thereto, which may occur to those skilled in the art, are to be considered within the scope of the invention as defined by the appended claims.

Example 1: preparation of 1% Xinjunan acetate and 80% folium artemisiae argyi flavonoid extract wettable powder

1% of Xinjunan acetate, 80% of folium artemisiae argyi flavonoid extract, 14% of sodium dodecyl benzene sulfonate and 5% of sodium lignosulfonate. Mixing the effective components with other auxiliary agents, mechanically crushing, then carrying out air flow crushing, and uniformly mixing to obtain 1% Xinjunan acetate and 80% folium artemisiae argyi flavonoid extract wettable powder.

Example 2: preparation of 8% ningnanmycin, 20% argyi leaf flavonoid extract aqua

8% of ningnanmycin, 20% of folium artemisiae argyi flavonoid extract, 10% of sodium dodecyl benzene sulfonate and water to be supplemented to 100%, and uniformly mixing to prepare 8% of ningnanmycin.20% of folium artemisiae argyi flavonoid extract aqua.

Example 3: preparation of microemulsion containing 25% prochloraz and 1% folium artemisiae argyi flavonoid extract

25% of prochloraz, 1% of folium artemisiae argyi flavonoid extract, 20% of aromatic hydrocarbon, 8% of alkylphenol polyoxyethylene, 5% of alcohol and water, wherein the water is added to the total amount of 100%. Dissolving prochloraz and folium artemisiae argyi flavonoid extract in aromatic hydrocarbon, mixing with alkylphenol ethoxylates and alcohol, slowly adding 41% of water, and stirring uniformly for 30 minutes to prepare the microemulsion of 25% prochloraz-1% folium artemisiae argyi flavonoid extract.

Example 4: preparation of 80% Bordeaux mixture, 1% Artemisia princeps flavone extract wettable powder

80% of Bordeaux mixture, 1% of folium artemisiae argyi flavonoid extract, 10% of sodium dodecyl benzene sulfonate and 9% of sodium lignosulphonate. Mixing the effective components with other adjuvants, mechanically pulverizing, jet milling, and mixing to obtain 80% Bordeaux mixture 1% wettable powder of folium Artemisiae Argyi flavonoid extract.

Example 5: preparation of 25% ningnanmycin and 15% folium artemisiae argyi flavonoid extract suspending agent

25% of ningnanmycin, 15% of folium artemisiae argyi flavonoid extract, 0.2% of xanthan gum, 3% of styrene acrylic acid copolymer, 4% of sodium dodecyl sulfate, 1% of magnesium aluminum silicate, 4% of glycerol, 0.2% of preservative, 0.2% of defoaming agent and the balance of water. And uniformly mixing the auxiliary agent and the active ingredients, grinding until the particle size is about 5 mu m, and filtering to obtain the suspending agent product.

Example 6: preparation of 20% kasugamycin and 15% folium artemisiae argyi flavonoid extract water dispersible granules

20% of kasugamycin, 15% of folium artemisiae argyi flavonoid extract, 10% of polycarboxylate, 10% of sodium dodecyl benzene sulfonate, 30% of glucose and 15% of ammonium sulfate, mixing the effective components with other auxiliary agents, mechanically crushing, then performing air flow crushing, and crushing ultrafine powder with the particle size of less than 10 microns. Adding a proper amount of water into the mixed material, and kneading for 15 minutes; and (3) extruding and granulating the kneaded wet material, and drying the wet granules in a fluidized bed dryer at 45 ℃ until the moisture content is less than 2% to obtain the 20% kasugamycin.5% folium artemisiae argyi flavonoid extract water dispersible granules.

Example 7: preparation of 10% pyraclostrobin-25% folium artemisiae argyi flavonoid extract suspending agent

10% of pyraclostrobin, 25% of folium artemisiae argyi flavonoid extract, 0.2% of xanthan gum, 3% of alkylphenol polyoxyethylene, 4% of sodium dodecyl sulfate, 1% of magnesium aluminum silicate, 0.2% of preservative, 0.2% of defoaming agent and the balance of water. And uniformly mixing the auxiliary agent and the active ingredients, grinding until the particle size is about 5 mu m, and filtering to obtain the suspending agent product.

Example 8: preparation of 10% difenoconazole and 5% folium artemisiae argyi flavonoid extract aqueous emulsion

10% of difenoconazole, 5% of folium artemisiae argyi flavonoid extract, 10% of aromatic hydrocarbon, 10% of alkylphenol polyoxyethylene, 5% of alcohol, 5% of urea, 0.2% of xanthan gum and the balance of water to 100%. Dissolving the difenoconazole and the folium artemisiae argyi flavonoid extract in aromatic hydrocarbon, mixing the aromatic hydrocarbon with alkylphenol ethoxylates and alcohol to obtain an oil phase, uniformly mixing urea and water to obtain a water phase, finally adding the water phase into the oil phase, stirring for 10 minutes, adding xanthan gum, stirring for 15 minutes, and homogenizing and emulsifying the mixed solution to obtain the 10% difenoconazole and 5% folium artemisiae argyi flavonoid extract aqueous emulsion.

Example 9: preparation of 50% propiconazole-8% folium artemisiae argyi flavonoid extract missible oil

50% of propiconazole, 8% of folium artemisiae argyi flavonoid extract, 15% of aromatic hydrocarbon, 5% of vegetable oil, 10% of fatty alcohol-polyoxyethylene ether and 12% of alkylphenol polyoxyethylene ether are uniformly mixed to prepare 50% propiconazole-8% folium artemisiae argyi flavonoid extract missible oil.

Application example 1:

table 11% Xinjunan acetate 80% folium artemisiae argyi flavonoid extract wettable powder and field efficacy test for preventing and treating citrus anthracnose

The test results (table 1) show that the wettable powder containing 1% Xinjunan acetate and 80% folium artemisiae argyi flavonoid extract has a good control effect on the citrus anthracnose.

Application example 2:

in-field efficacy test of water dispersible granules containing 220% kasugamycin and 15% folium artemisiae argyi flavonoid extract for preventing and treating peach gummosis

The test results (table 2) show that the water dispersible granule of 20% kasugamycin and 15% folium artemisiae argyi flavonoid extract has a good prevention effect on peach gummosis.

Application example 3:

in-field efficacy test of table 310% difenoconazole and 5% folium artemisiae argyi flavonoid extract water emulsion for preventing and treating citrus scab

The test results (table 3) show that the aqueous emulsion of 10% difenoconazole and 5% folium artemisiae argyi flavonoid extract has better prevention effect on citrus scab.

Application example 4:

table 48% ningnanmycin, 20% argy wormwood leaf flavonoid extract aqueous solution field efficacy test for preventing and treating kiwifruit canker

The test results (table 4) show that the aqua containing 8% ningnanmycin and 20% folium artemisiae argyi flavonoid extract has better prevention effect on kiwifruit canker.

Application example 5:

table 525% Prochloraz 1% folium Artemisiae Argyi flavonoid extract microemulsion field efficacy test for preventing and treating cucumber powdery mildew

The test results (table 5) show that the microemulsion containing 25% of prochloraz and 1% of folium artemisiae argyi flavonoid extract has a good control effect on cucumber powdery mildew.

Application example 6:

field efficacy test of table 650% propiconazole and 8% folium artemisiae argyi flavonoid extract emulsifiable concentrate for preventing and treating sigatoka

The test results (table 6) show that the 50% propiconazole-8% folium artemisiae argyi flavonoid extract emulsifiable concentrate has a good prevention effect on the sigatoka.