阅读说明：本技术 视网膜及视神经保护性电刺激装置 (Retina and optic nerve protective electric stimulation device ) 是由 梁婷 于 2020-10-13 设计创作，主要内容包括：本发明属于医疗装置技术领域,尤其涉及一种视网膜及视神经保护性电刺激装置,所述刺激器输出的刺激脉冲通过放大电极跨视网膜和跨眼眶电刺激于视觉传导通路上的神经组织,本发明解决了现有技术存在缺少基于无损伤的眼部跨视网膜电刺激和跨眼眶电刺激及其电刺激装置的研究的问题,具有填补了无损伤眼部跨视网膜和跨眼眶电刺激装置应用的空白的有益技术效果。(The invention belongs to the technical field of medical devices, and particularly relates to a retina and optic nerve protective electrical stimulation device.)

1. The retina and optic nerve protective electrical stimulation device is characterized by comprising a stimulator, wherein the stimulation pulse output by the stimulator is used for electrically stimulating nerve tissues on a visual conduction path across the retina and the orbit through an amplifying electrode.

2. The device of claim 1, wherein the stimulator outputs the corresponding stimulation pulses by automatically controlling the stimulation time and adjusting the pulse width, frequency and amplitude of the pulse waveform.

3. The device of claim 1 wherein the magnifying electrode comprises a set of recovery electrodes and a set of stimulating electrodes, the set of recovery electrodes comprising a recovery electrode affixed to the underside of the left orbit and another recovery electrode affixed to the underside of the right orbit, the set of stimulating electrodes comprising one stimulating electrode affixed to the top of the left orbit and another stimulating electrode affixed to the top of the right orbit.

4. The device of claim 3, wherein the one stimulating electrode and the one recovery electrode are disposed on an inner surface of a left cover of an eye mask, and the other stimulating electrode and the other recovery electrode are disposed on an inner surface of a right cover of an eye mask.

5. The device of claim 2, wherein the stimulator includes a stimulation circuit including a programmable control chip U1;

the output of the first control end of the programmable control chip U1 is connected with a stimulation pulse generating circuit A1;

the output of the second control end of the programmable control chip U1 is connected to a recovery pulse generating circuit A2;

the output of the third control end of the programmable control chip U1 is connected to another stimulation pulse generating circuit A3;

the output of the fourth control end of the programmable control chip U1 is connected to another recovery pulse generating circuit A4.

6. The device of claim 5, wherein the one stimulation pulse generation circuit A1 and the other stimulation pulse generation circuit A3 both use the same stimulation pulse generation circuit;

the stimulation pulse generating circuit comprises an adjustable resistor R1, one end of the adjustable resistor R1 is connected to a positive power supply, the other end of the adjustable resistor R1 is connected to a first control end of a programmable control chip U1 or a third control end of a programmable control chip U1, one end of a capacitor C1 is connected to the base of a transistor Q1, the other end of the capacitor C1 is grounded, a collector of the transistor Q1 is connected to the positive power supply through a resistor R2 and is connected to the base of a transistor Q2 through a resistor R3, an emitter of the transistor Q1 is grounded, the resistor R3 is grounded in series with a resistor R5, a collector of the transistor Q2 is connected to one end of a resistor R4 and is connected to a stimulation electrode or another stimulation electrode, the other end of the resistor R4 is connected to the positive power supply, and an emitter of the transistor Q2 is grounded.

7. The apparatus of claim 5, wherein the recovery pulse generating circuit A2 and the recovery pulse generating circuit A4 both use the same recovery pulse generating circuit;

the recovery pulse generating circuit comprises an adjustable resistor R1 ', one end of the adjustable resistor R1' is connected with the ground, the other end of the second switch is connected to the first control end of the programmable control chip U1 or the third control end of the programmable control chip U1, and is connected to one end of the capacitor C1 ' and to the base of the transistor Q1 ', the other end of the capacitor C1 ' is connected to the negative power supply, the collector of the transistor Q1 ' is connected to ground via the resistor R2 ', and is connected with the base electrode of a transistor Q2 ' through a resistor R3 ', the emitter electrode of the transistor Q1 ' is connected with a negative power supply, the resistor R3 'is connected with a negative power supply through being connected with a resistor R5' in series, the collector of the transistor Q2 'is connected with one end of a resistor R4', and is connected with a recovery electrode or another recovery electrode through a phase-shifting capacitor C2, the other end of the resistor R4 'is connected with the ground, and the emitter of the transistor Q2' is connected with a negative power supply.

8. The device of claim 5, wherein the programmable control chip U1 employs a digital signal processor DSP AVP32F 335.

9. The device according to claim 2, wherein the preferred embodiment of the stimulation pulse is a biphasic square pulse with a frequency of 20Hz, a stimulation time of 60 minutes, a stimulation current of 100 μ a and a pulse width of 1 ms/phase.

Technical Field

The invention belongs to the technical field of medical devices, and particularly relates to a retina and optic nerve protective electrical stimulation device.

Background

Traumatic optic nerve injury can cause retrograde apoptosis of retinal ganglion cells, while transcorneal electrical stimulation can increase cell survival. We monitored the morphology and survival of retinal ganglion cells after optic nerve injury in live animals by using the retinal confocal method. Optic nerve injury was performed in rats and retinal ganglion cell numbers and morphology were recorded before injury and 3, 7, and 15 days after injury. 3 days after optic nerve injury, the transcorneal electrical stimulation was used to find that a large number of retinal ganglion cells survived in the stimulated group compared with the sham stimulated group, the difference between the two became small at seven days, and the difference between the two disappeared at 15 days. Morphological analysis shows that the average cell morphology of the pseudo-stimulation group is obviously changed in the early stage of injury, most cells are subjected to edema and apoptosis, and the cell morphology of the stimulation group is not obviously changed. Therefore, compared with a pseudo-stimulation group, the transcorneal electrical stimulation can protect damaged cells, maintain normal cell morphology and improve the survival rate of the damaged cells.

Under the condition of separation, the optic nerve separated by electric stimulation can promote axon regeneration and improve the survival of retinal ganglion cells, and research results show that the electric stimulation has the optic nerve protection effect. In clinic, optic nerve damage frequently occurs in a closed state, particularly, partial damage is frequently seen, and the completely-isolated optic nerve damage under experimental conditions cannot well simulate clinical conditions, so related researches of the optic nerve damage are further improved.

The retina and optic nerve are electrically stimulated as an emerging physical therapy, which can activate the visual system (retina and optic nerve) and has a protective effect on damaged neurons. This becomes the basis that the electrostimulation device can be applied in ophthalmology clinic, and it is expected to be able to treat a variety of retinal and optic nerve diseases, such as: retinitis pigmentosa, traumatic optic neuropathy, anterior ischemic optic neuropathy, and retinal artery occlusion. Many studies are currently being conducted to elucidate the mechanism of action of the electrically stimulated visual system, mostly using transcorneal electrical stimulation or direct electrical stimulation of damaged optic nerves in the open state, but both methods have drawbacks: first, transcorneal electrical stimulation, in which the stimulation electrodes need to be applied to the corneal surface under topical anesthesia, is not tolerated by patients for long periods of time. In clinical practice, direct electrical stimulation of the damaged optic nerve and retina in the open state cannot be achieved.

In summary, the research based on non-invasive electrical stimulation of the eye across the retina or across the orbit and the electrical stimulation device thereof has important significance for the development of ophthalmic medicine.

Disclosure of Invention

The invention provides a retina and optic nerve protective electrical stimulation device, which aims to solve the problem that the research based on non-invasive eye transretinal electrical stimulation or transorbital electrical stimulation and the electrical stimulation device thereof in the prior art proposed in the background technology has important significance for the development of ophthalmic medicine.

The technical problem solved by the invention is realized by adopting the following technical scheme that the retina and optic nerve protective electrical stimulation device comprises a stimulator, wherein a stimulation pulse output by the stimulator is electrically stimulated on nerve tissues on a visual conduction path across the retina or the orbit through an amplifying electrode.

Further, the stimulator outputs corresponding stimulation pulses by automatically controlling the stimulation time and adjusting the pulse waveform, pulse width, frequency and amplitude.

Further, the magnifying electrode comprises a recovery electrode group and a stimulation electrode group, the recovery electrode group comprises a recovery electrode attached below the left eye socket and another recovery electrode attached below the right eye socket, and the stimulation electrode group comprises a stimulation electrode attached above the left eye socket and another stimulation electrode attached above the right eye socket.

Furthermore, the stimulating electrode and the recovery electrode are distributed on the inner surface of a left cover of an eye cover, and the other stimulating electrode and the other recovery electrode are distributed on the inner surface of a right cover of the eye cover.

Further, the stimulator includes a stimulation circuit including a programmable control chip U1;

the output of the first control end of the programmable control chip U1 is connected with a stimulation pulse generating circuit A1;

the output of the second control end of the programmable control chip U1 is connected to a recovery pulse generating circuit A2;

the output of the third control end of the programmable control chip U1 is connected to another stimulation pulse generating circuit A3;

the output of the fourth control end of the programmable control chip U1 is connected to another recovery pulse generating circuit A4.

Furthermore, the same stimulation pulse generating circuit is adopted by the one stimulation pulse generating circuit A1 and the other stimulation pulse generating circuit A3;

the stimulation pulse generating circuit comprises an adjustable resistor R1, one end of the adjustable resistor R1 is connected to a positive power supply, the other end of the adjustable resistor R1 is connected to a first control end of a programmable control chip U1 or a third control end of a programmable control chip U1, one end of a capacitor C1 is connected to the base of a transistor Q1, the other end of the capacitor C1 is grounded, a collector of the transistor Q1 is connected to the positive power supply through a resistor R2 and is connected to the base of a transistor Q2 through a resistor R3, an emitter of the transistor Q1 is grounded, the resistor R3 is grounded in series with a resistor R5, a collector of the transistor Q2 is connected to one end of a resistor R4 and is connected to a stimulation electrode or another stimulation electrode, the other end of the resistor R4 is connected to the positive power supply, and an emitter of the transistor Q2 is grounded.

Furthermore, the recovery pulse generating circuit A2 and the other recovery pulse generating circuit A4 both adopt the same recovery pulse generating circuit;

the recovery pulse generating circuit comprises an adjustable resistor R1 ', one end of the adjustable resistor R1' is connected with the ground, the other end of the second switch is connected to the first control end of the programmable control chip U1 or the third control end of the programmable control chip U1, and is connected to one end of the capacitor C1 ' and to the base of the transistor Q1 ', the other end of the capacitor C1 ' is connected to the negative power supply, the collector of the transistor Q1 ' is connected to ground via the resistor R2 ', and is connected with the base electrode of a transistor Q2 ' through a resistor R3 ', the emitter electrode of the transistor Q1 ' is connected with a negative power supply, the resistor R3 'is connected with a negative power supply through being connected with a resistor R5' in series, the collector of the transistor Q2 'is connected with one end of a resistor R4', and is connected with a recovery electrode or another recovery electrode through a phase-shifting capacitor C2, the other end of the resistor R4 'is connected with the ground, and the emitter of the transistor Q2' is connected with a negative power supply.

Further, the programmable control chip U1 employs a digital signal processor DSP AVP32F 335.

Further, a preferred embodiment of the stimulation pulse is a biphasic square pulse with a frequency of 20Hz, a stimulation time of 60 minutes, a stimulation current of 100 μ a and a pulse width of 1 ms/phase.

Further, the stimulating electrode is a metal ring with the diameter of 3 mm.

The beneficial technical effects are as follows:

the stimulation pulse output by the stimulator is electrically stimulated to nerve tissues on a visual conduction path across the retina or across the orbit through the amplifying electrode, and the result of the preclinical research using the cross-corneal electrical stimulation shows that the physical therapy of the electrical stimulation is suitable for early intervention after injury, and in the early stage after the optic nerve is injured, the cross-corneal electrical stimulation can obviously improve the survival rate of retinal ganglion cells, protect the function of residual optic nerve fibers, and provide possibility for the structural reconstruction of the damaged nerve fibers. However, the observation of protective effects of transcorneal electrical stimulation on the retina and optic nerve is limited to the early stages after injury, at which time retinal ganglion cell death has not yet fully occurred. In order to know the long-term effect, an eye electrical stimulation device which is free of damage, tolerant, portable, simple and easy to operate is urgently needed to be matched with the long-term electrical stimulation observation. Therefore, the invention provides a retina and optic nerve protective electrical stimulation device, which fills the blank of the application of a non-destructive portable eye cross-retina and cross-orbit electrical stimulation device.

Drawings

FIG. 1 is a schematic view of the structure of the apparatus;

FIG. 2 is a block diagram of the stimulation circuitry of the present device;

FIG. 3 is a circuit diagram of a stimulus pulse generation circuit of the present device;

FIG. 4 is a circuit diagram of a recovery pulse generating circuit of the present apparatus;

FIG. 5 is a schematic diagram of the application of the present apparatus;

Detailed Description

The invention is further described below with reference to the accompanying drawings:

in the figure:

1-a stimulator; 2-amplifying electrode, 3-recovering electrode, 4-stimulating electrode, 5-stimulating electrode, 6-recovering electrode, 7-another stimulating electrode, 8-another recovering electrode, 9-stimulating electrode group, 10-recovering electrode group, 11-eye mask;

u1-programmable control chip; a1-a stimulation pulse generating circuit; a2-a recovery pulse generating circuit; a 3-another stimulus pulse generating circuit; a 4-another recovery pulse generating circuit; r1-adjustable resistance; c1-capacitance; q1-transistor; r2-resistance; r3-resistance; q2-transistor; r5-resistance; r4-resistance;

r1' -adjustable resistance; c1' -capacitance; a Q1' -transistor; r2' -resistance; r3' -resistance; a Q2' -transistor; r5' -resistance; r4' -resistance.

Example (b):

in this embodiment: as shown in figure 1, the retina and optic nerve protective electrical stimulation device comprises a stimulator 1, wherein stimulation pulses output by the stimulator 1 electrically stimulate nerve tissues on a visual conduction path across the retina or across the orbit through an amplifying electrode 2.

The stimulator 1 outputs corresponding stimulation pulses by automatically controlling the stimulation time and adjusting the pulse waveform, the pulse width, the frequency and the amplitude.

Since the stimulation pulses output by the stimulator are stimulated across the retina or across the orbit by the magnifying electrodes to the nerve tissue on the visual conduction path, since preclinical studies using transcorneal electrical stimulation show that this treatment is also suitable for intervention after early injury, it was first proposed that transcorneal electrical stimulation can significantly increase the number of retinal ganglion cells after optic nerve dissection and elaborates the stimulation parameters that produce the best neuroprotection, transcorneal electrical stimulation was applied after optic nerve crush injury, the injury model provides opportunities for residual nerve fiber functional recovery, axonal structural recovery is possible, interestingly this approach leads to functional recovery, however the authors only observed six days after injury, at which time retinal ganglion cell death has not yet fully occurred, in order to elaborate these observations, beyond the ability to improve the environment, transcorneal electrical stimulation could support long-term survival of retinal ganglion cells within the first week after injury, in order to understand this long-term effect, by carrying out electric stimulation on the injured rat immediately and carrying out electric stimulation on the injured rat for a period of time, and repeatedly observing retinal ganglion cells in the same animal body in real time by using an in-vivo confocal nerve image method, the invention is based on the research result, the stimulator sends out stimulation pulses, then the stimulation current of the stimulation pulses is output to the electrode, the electrode acts on the surface of the skin around the retina after the current is amplified, thereby stimulating the nerve tissue on the visual conduction path to achieve the effect of regenerating retinal ganglion cells, therefore, the invention provides a retina and optic nerve protective electrical stimulation device, which fills the blank of the application of the non-injury eye cross-retina and cross-orbit electrical stimulation device.

As shown in fig. 5, the magnifying electrode 2 includes a recovery electrode group 10 and a stimulation electrode group 9, the recovery electrode group 10 includes a recovery electrode 6 attached to the lower portion of the left eye socket and another recovery electrode 8 attached to the lower portion of the right eye socket, and the stimulation electrode group 9 includes a stimulation electrode 5 attached to the upper portion of the left eye socket and another stimulation electrode 7 attached to the upper portion of the right eye socket.

Because the magnifying electrode comprises the recovery electrode group and the stimulating electrode group, the recovery electrode group comprises a recovery electrode attached under the left eye socket and another recovery electrode attached under the right eye socket, the stimulating electrode group comprises one stimulating electrode attached above the left eye socket and the other stimulating electrode attached above the right eye socket, because the eye mask is internally provided with four electrodes in total, two stimulation electrodes and two recovery electrodes, after the eye mask is worn on a human face, the positions of the electrodes are respectively positioned above and below the eye sockets, since the stimulation electrodes are distributed above and below the both eye sockets, the current enters the nerve tissue on the visual conduction path through the upper part of the eye sockets, under passage through the orbit, a long-term survival effect of retinal ganglion cells by transcorneal electrical stimulation is achieved.

The stimulating electrode 5 and the recovery electrode 6 are distributed on the inner surface of the left cover of an eye cover 11, and the other stimulating electrode 7 and the other recovery electrode 8 are distributed on the inner surface of the right cover of the eye cover 11.

The stimulating electrode and the recycling electrode are distributed on the inner surface of the left cover of the eye cover, the other stimulating electrode and the other recycling electrode are distributed on the inner surface of the right cover of the eye cover, and the stimulating electrode and the recycling electrode are packaged on the corresponding inner surfaces of the eye cover by taking the eye cover as a carrier.

As shown in fig. 2, the stimulator 1 includes a stimulation circuit including a programmable control chip U1;

the output of the first control end of the programmable control chip U1 is connected with a stimulation pulse generating circuit A1;

the output of the second control end of the programmable control chip U1 is connected to a recovery pulse generating circuit A2;

the output of the third control end of the programmable control chip U1 is connected to another stimulation pulse generating circuit A3;

the output of the fourth control end of the programmable control chip U1 is connected to another recovery pulse generating circuit A4.

The stimulator comprises a stimulation circuit which comprises a programmable control chip U1, a stimulation pulse generating circuit A1, a recovery pulse generating circuit A2, another stimulation pulse generating circuit A3 and another recovery pulse generating circuit A4, wherein the circuits are distributed in an array structure and are connected with one another, control signals are respectively output to the stimulation pulse generating circuits A1 and A3 and the recovery pulse generating circuits A2 and A4 through the control chip U1, the stimulation pulse generating circuits and the recovery pulse generating circuits output biphasic square pulses, parameters of the biphasic square pulses are 1ms/phase, the frequency is 20Hz, and the energy is 100 muA, and the topological structure of the module adopts a distributed array structure, so that the synchronous structure is kept and the synchronous structure is independent from one another, and the circuits are prevented from mutual crosstalk through the isolation circuits.

As shown in fig. 3, the same stimulation pulse generation circuit is used for both the one stimulation pulse generation circuit a1 and the other stimulation pulse generation circuit A3;

the stimulation pulse generating circuit comprises an adjustable resistor R1, one end of the adjustable resistor R1 is connected to a positive power supply, the other end of the adjustable resistor R1 is connected to a first control end of a programmable control chip U1 or a third control end of a programmable control chip U1, one end of a capacitor C1 is connected to the base of a transistor Q1, the other end of the capacitor C1 is grounded, the collector of the transistor Q1 is connected to the positive power supply through a resistor R2 and is connected to the base of a transistor Q2 through a resistor R3, the emitter of the transistor Q1 is grounded, the resistor R3 is grounded in series with a resistor R5, the collector of the transistor Q2 is connected to one end of a resistor R4 and is connected to a stimulation electrode 5 or another stimulation electrode 7, the other end of the resistor R4 is connected to the positive power supply, and the emitter of the transistor Q2 is grounded.

As shown in fig. 4, the recovery pulse generating circuit a2 and the recovery pulse generating circuit a4 both use the same recovery pulse generating circuit;

the recovery pulse generating circuit comprises an adjustable resistor R1 ', one end of the adjustable resistor R1' is connected with the ground, the other end of the second switch is connected to the first control end of the programmable control chip U1 or the third control end of the programmable control chip U1, and is connected to one end of the capacitor C1 ' and to the base of the transistor Q1 ', the other end of the capacitor C1 ' is connected to the negative power supply, the collector of the transistor Q1 ' is connected to ground via the resistor R2 ', and is connected with the base electrode of a transistor Q2 ' through a resistor R3 ', the emitter electrode of the transistor Q1 ' is connected with a negative power supply, the resistor R3 'is connected with a negative power supply through being connected with a resistor R5' in series, the collector of the transistor Q2 'is connected with one end of a resistor R4', and is connected with a recovery electrode 6 or another recovery electrode 8 through a phase-shifting capacitor C2, the other end of the resistor R4 'is connected with the ground, and the emitter of the transistor Q2' is connected with a negative power supply.

Because the same stimulation pulse generating circuit is adopted by the one stimulation pulse generating circuit A1 and the other stimulation pulse generating circuit A3, and the same recovered pulse generating circuit is adopted by the one recovered pulse generating circuit A2 and the other recovered pulse generating circuit A4, because the recovery pulse generating circuit comprises a unijunction transistor Q, a resistor R, a potentiometer and a capacitor C, an oscillator comprises a triode Q and a Q' amplifier, the potentiometer is adjusted, the oscillation frequency of the relaxation oscillator can be changed, a time base pulse of 0.1 ms-15 s is obtained, the pulse is amplified by the amplifier and then outputs a square wave pulse which is used as an input signal of a time counter, the important point is that the stimulation pulse generating circuit A1 does not have the phase shifting capacitor C, the recovered pulse generating circuit A2 has the phase shifting capacitor C, the two circuits only have the phase shifting capacitor C, and the phase shifting capacitor C just separates the stimulation pulse from the recovered pulse by one phase, due to the adoption of the bidirectional pulse circuit, a two-phase square pulse is formed, and the two-phase square pulse stimulates the optic nerve up and down together, so that the stimulation effect is improved.

The programmable control chip U1 employs a digital signal processor DSP AVP32F 335.

Because the programmable control chip U1 adopts a digital signal processor DSP AVP32F335, because the AVP32F335AVP32F335 floating point type DSP adopts a static CMOS technology, the system main frequency reaches 120MHz (the upgrade version reaches 250Mhz), the 3.3V io design but the kernel adopts 1.5V, the Harvard structure is adopted, the single precision floating point operation FPU is integrated, and a standard mathematical calculation table is provided. 1.5VLDO is integrated internally while media access control is supported. On the peripheral, a DMA (direct memory access) with 6 channels is adopted, PWM (pulse width modulation) supports 18 paths of output, 6 time input capture is realized, and an ADC (analog to digital converter) has 16 channels. Two orthogonal coding interfaces support 3-path 32-bit system timers on timer resources, and 17 universal timers are additionally arranged. On a communication interface, a CAN transceiver is integrated inside, an upgraded chip also supports an Ethercat bus transceiver, and 176 recommended BGA and LQFP are packaged.

The preferred embodiment of the stimulation pulse is a biphasic square pulse with a frequency of 20Hz, a stimulation time of 60 minutes, a stimulation current of 100 pa and a pulse width of 1 ms/phase.

Because the stimulator is adopted to output corresponding stimulation pulses by automatically controlling the stimulation time and adjusting the pulse width, the frequency and the amplitude of the pulse waveform, the preferred scheme of the stimulation pulses is biphasic square pulses, the frequency of the biphasic square pulses is 20Hz, the stimulation time is 60 minutes, the stimulation current is 100 muA, and the pulse width is 1 ms/phase. Meanwhile, a remote controller is provided, the remote controller can adjust the electric parameters output by the electrodes on the eyeshade and control the stimulation time, and if the patient does not want to adjust the parameters by himself, some stimulation parameters corresponding to different diseases are stored in the machine and can be selected directly.

The stimulating electrode 4 is a metal ring with the diameter of 3 mm.

The amplification electrode comprises a recovery electrode and a stimulation electrode, the recovery electrode is attached to the surface of skin, the stimulation electrode is attached to the surface of cornea, the stimulation electrode is a metal ring with the diameter of 3mm, and the two-phase square pulse, 1ms/phase,20Hz and 100 muA are used for stimulating for 60 minutes. Electrical stimulation was applied to the ear at that time after injury, and 11 days after injury, with the stimulation electrode being a 3mm diameter metal ring and the reference electrode fixed to the ear.

The working principle is as follows:

the present patent shows that the treatment is also suitable for intervention after early injury through the preclinical research results using transcorneal electrical stimulation, which firstly proposes that transcorneal electrical stimulation can significantly increase the number of retinal ganglion cells after the optic nerve is cut off, and specifies the stimulation parameters capable of producing the best neuroprotective effect, and the transcorneal electrical stimulation is applied after optic nerve crush injury, the injury model provides the opportunity for the functional recovery of the residual nerve fibers, the recovery on axon structure provides the possibility, interestingly, the method leads to the functional recovery, however, the authors only observe the situation six days after injury, and the death of retinal ganglion cells does not completely occur at this time, to elaborate on these observations, in the first week after injury, whether transcorneal electrical stimulation can support long-term survival of retinal ganglion cells can be improved, in order to understand the long-term effect, by performing electrical stimulation immediately on the injured rat, and performing electrical stimulation for a period of time after injury, using an in vivo confocal nerve image method, retinal ganglion cells are repeatedly observed in the same animal body in real time The problem is solved, and the beneficial technical effects of filling the blank of the application of the non-injury, simple operation and portable eye cross-retina and cross-orbit electric stimulation device are achieved.

The technical solutions of the present invention or similar technical solutions designed by those skilled in the art based on the teachings of the technical solutions of the present invention are all within the scope of the present invention to achieve the above technical effects.