阅读说明：本技术 一种便携式植物叶片的碳同位素标记装置 (Carbon isotope labeling device of portable plant leaf ) 是由 未晓巍 穆春生 严明 张金伟 杨雨衡 韩佳玉 于 2020-12-18 设计创作，主要内容包括：本发明公开了一种便携式植物叶片的碳同位素标记装置,它包括：瓶身主体、上盖；所述的瓶身主体上设有单项透气阀、第一充气管、第二充气管,单项透气阀与第一充气管、第二充气管分别设置瓶身主体两侧；上盖包括：主盖、副盖、密封组件；副盖通过密封组件与主盖密封连接；主盖和副盖密封连接处设有叶茎通孔；将被标记的植物枝叶叶茎上用密封绑带进行密封缠绕后,能在叶茎通孔紧密贴合；对放有植物叶片的瓶中气体进行氮气冲换,冲换完毕后,在进行标记用的常温～(13)CO-2充入,完成碳同位素标记；此种方式减小了标记时标记瓶身内的气体及温度等因素对植物叶片碳同位素标记时的影响,提高了对植物叶片进行稳定性碳同位素的效率和可靠性。(The invention discloses a portable carbon isotope labeling device of plant leaves, which comprises: a bottle body main body and an upper cover; the bottle body main body is provided with a single vent valve, a first inflation tube and a second inflation tube, and the single vent valve, the first inflation tube and the second inflation tube are respectively arranged at two sides of the bottle body main body; the upper cover includes: the main cover, the auxiliary cover and the sealing assembly; the auxiliary cover is hermetically connected with the main cover through a sealing component; the sealed joint of the main cover and the auxiliary cover is provided with a leaf and stem through hole; after the marked plant branches, leaves, stems and leaves are sealed and wound by a sealing binding band, the marked plant branches, leaves, stems and leaves can be tightly attached to the through holes of the leaves and stems; the gas in the bottle with plant leaves is flushed and exchanged with nitrogen, and after the flushing and exchanging are finished, the gas is marked at normal temperature 13 CO 2 Filling to finish carbon isotope labeling; the method reduces the influence of the factors such as gas and temperature in the bottle body on the carbon isotope labeling of the plant leaves during the labeling, and improves the stability of the plant leavesEfficiency and reliability of carbon isotopes.)

1. A portable carbon isotope labeling device for plant leaves, comprising: a bottle body main body (1) and an upper cover (2);

the method is characterized in that:

the bottle body main body (1) is provided with a single vent valve (12), a first inflation tube (13) and a second inflation tube (14), and the single vent valve (12), the first inflation tube (13) and the second inflation tube (14) are respectively arranged at two sides of the bottle body main body (1);

the upper cover (2) comprises: a main cover (21), an auxiliary cover (22), and a seal assembly (23); the auxiliary cover (22) is connected with the main cover (21) in a sealing way through a sealing component (23); the sealed joint of the main cover (21) and the auxiliary cover (22) is provided with a leaf and stem through hole.

2. The carbon isotope labeling apparatus of a portable plant leaf as set forth in claim 1, wherein: the main cover (21) is provided with a gap part (211); one side of the gap part (211) is provided with a clamping groove opening I (212);

the auxiliary cover (22) is provided with a sealing part (221), a clamping groove opening II (222) and an axle connecting hole (223); the sealing part (221) is arranged at one end of the auxiliary cover (22); the clamping groove opening II (222) is arranged on the vertical surface of one side of the sealing part (221);

the blade-stem through hole is mainly formed by butt joint of a clamping groove opening I (212) and a clamping groove opening II (222).

3. The carbon isotope labeling apparatus of a portable plant leaf as set forth in claim 2, wherein: said sealing assembly (23) comprising: the connecting rod (231), the sealing rubber gasket (232), the spring (233), the gasket (234), the snap spring (235) and the knob (236);

the sealing rubber gasket (232) is sleeved at the lower end of the connecting rod (231) and then sequentially coupled with the auxiliary cover (22) and the main cover (21) in a shaft way; the gasket (234) is fixed on the upper part of the connecting rod (231) through a clamp spring (235); the spring (233) is sleeved in the connecting rod (231), so that the lower contact surface of the spring (233) abuts against the upper end of the center of the main cover (21), and the upper contact surface of the spring (233) abuts against the lower contact surface of the gasket (234); the knob (236) is tightly connected with the top of the connecting rod (231).

Technical Field

The invention relates to the technical field of stable isotope labeling, in particular to a portable plant leaf carbon isotope labeling device and a plant leaf carbon isotope labeling device.

Background

Carbon in the plant is used as a main element of plant metabolism and participates in the process of plant photosynthesis, carbon isotope labeling can be used as an important way for evaluating the physiological mechanism of the plant, and carbon isotope metabolism can be used as an important index for analyzing the physiological mechanism of the plant.

When plants are subjected to stable carbon isotope labeling, the plants generally perform photosynthesis on a sunny day under the condition of sufficient illumination, and simultaneously, the plants hydrate carbon dioxide and water into energy-rich organic matters and release oxygen. If the plants absorb the labeled carbon dioxide gas (e.g. stable isotope) during photosynthesis¹³CO₂) Namely, the labeling of stable isotopes is completed.

The marking of the plant leaves adopts a mode of shielding tissues of other parts in the past, and can not ensure the absorption of stable carbon isotopes during the respiration action of plants, so that a convenient and accurate method for marking the stable carbon isotopes of the plant leaves is designed, and the technical problem to be solved is urgently needed.

Disclosure of Invention

The invention aims to solve the problems and provides a portable plant leaf carbon isotope labeling device and a plant leaf carbon isotope labeling method;

a portable carbon isotope labeling device for plant leaves, comprising: a bottle body main body 1 and an upper cover 2;

the method is characterized in that:

the bottle body main body 1 is provided with a single vent valve 12, a first inflation tube 13 and a second inflation tube 14, and the single vent valve 12, the first inflation tube 13 and the second inflation tube 14 are respectively arranged at two sides of the bottle body main body 1;

the upper cover 2 comprises: a main cover 21, an auxiliary cover 22, a seal assembly 23; the auxiliary cover 22 is connected with the main cover 21 in a sealing mode through a sealing assembly 23; the main cover 21 and the auxiliary cover 22 are provided with leaf and stem through holes at the sealed connection part.

The main cover 21 is provided with a gap part 211; one side of the gap part 211 is provided with a clamping groove opening I212;

the auxiliary cover 22 is provided with a sealing part 221, a clamping groove opening II 222 and an axle connecting hole 223; the sealing part 221 is arranged at one end of the auxiliary cover 22; the clamping groove opening II 222 is formed in the vertical surface of one side of the sealing part 221;

the blade-stem through hole is mainly formed by butt joint of a clamping groove opening I212 and a clamping groove opening II 222.

Said seal assembly 23 comprises: the connecting rod 231, the sealing rubber gasket 232, the spring 233, the gasket 234, the snap spring 235 and the knob 236;

the sealing rubber gasket 232 is sleeved at the lower end of the connecting rod 231 and then sequentially coupled with the auxiliary cover 22 and the main cover 21; the gasket 234 is fixed on the upper part of the connecting rod 231 through a clamp spring 235; the spring 233 is sleeved in the connecting rod 231, so that the lower contact surface of the spring 233 abuts against the upper end of the center of the main cover 21, and the upper contact surface of the spring 233 abuts against the lower contact surface of the gasket 234; the knob 236 is closely coupled to the top of the connection rod 231.

The invention discloses a portable carbon isotope labeling device of plant leaves, which comprises: a bottle body main body 1 and an upper cover 2; the bottle body main body 1 is provided with a single vent valve 12, a first inflation tube 13 and a second inflation tube 14, and the single vent valve 12, the first inflation tube 13 and the second inflation tube 14 are respectively arranged at two sides of the bottle body main body 1; the upper cover 2 includes: a main cover 21, an auxiliary cover 22, a seal assembly 23; the auxiliary cover 22 is connected with the main cover 21 in a sealing mode through a sealing assembly 23; the sealed joint of the main cover 21 and the auxiliary cover 22 is provided with a leaf and stem through hole; after the marked plant branches, leaves, stems and leaves are sealed and wound by a sealing binding band, the marked plant branches, leaves, stems and leaves can be tightly attached to the through holes of the leaves and stems; the gas in the bottle with plant leaves is flushed and exchanged with nitrogen, and after the flushing and exchanging are finished, the gas is marked at normal temperature¹³CO₂Filling to finish carbon isotope labeling; this reduces the gas in the bottle body during labelingThe influence of factors such as body and temperature on the carbon isotope labeling of the plant leaves improves the efficiency and reliability of stabilizing the carbon isotope labeling of the plant leaves.

Drawings

FIG. 1 is a schematic view of the entire portable plant leaf carbon isotope labeling apparatus of the present invention;

FIG. 2 is a schematic cross-sectional view of a main body of a carbon isotope labeling apparatus for portable plant leaves according to the present invention;

FIG. 3 is a schematic view of a connecting rod of the carbon isotope labeling apparatus of a portable plant leaf of the present invention;

FIG. 4 is an exploded top view of the carbon isotope labeling apparatus of portable plant leaves according to the present invention;

FIG. 5 is a schematic illustration of the upper cover of the carbon isotope labeling apparatus of the portable plant leaves of the present invention;

fig. 6 is a schematic view of the upper cover clamping the leaves of the carbon isotope labeling apparatus of the portable plant leaves of the present invention.

Detailed Description

Embodiment 1A portable carbon isotope labeling device of plant leaves

Referring to fig. 1 to 4, a carbon isotope labeling apparatus for portable plant leaves, comprising: a bottle body main body 1 and an upper cover 2;

the upper end of the bottle body main body 1 is provided with a bottle opening 11, and an internal thread opening 111 is arranged at the bottle opening 11;

the bottle body main body 1 is provided with a single vent valve 12, a first inflation tube 13 and a second inflation tube 14, and the single vent valve 12, the first inflation tube 13 and the second inflation tube 14 are respectively arranged at two sides of the bottle body main body 1;

the first inflation control valve 131 is arranged on the first inflation tube 13, and a first inflation nozzle 132 is arranged at one end of the first inflation tube 13;

a second inflation control valve 141 is arranged on the second inflation pipe 14; a second inflation nozzle 142 is provided at one end of the second inflation tube 14;

the bottle body main body 1 is also provided with a hanging and connecting ring 15, and the bottle body main body 1 is hung and connected on branches and tendrils of marked plants through the hanging and connecting ring 15;

the upper cover 2 comprises: a main cover 21, an auxiliary cover 22, a seal assembly 23;

the main cover 21 is circular with a notch 211, and the notch 211 occupies about one fourth of the whole body; one side of the gap part 211 is provided with a clamping groove opening I212; the vertical surface of the notch part 211 is an inner inclined surface;

the lower edge of the outer ring of the main cover 21 is provided with a thread I2141; the middle part of the main cover 21 is provided with a shaft hole;

the auxiliary cover 22 is provided with a sealing part 221, a clamping groove opening II 222 and a shaft connecting hole 223;

the sealing part 221 is arranged at one end of the auxiliary cover 22, and the vertical surface of the sealing part 221 is an outer inclined surface; the clamping groove opening II 222 is formed in the vertical surface of one side of the sealing part 221;

the vertical surface of the sealing part 221 is an outer inclined surface; the external slope of the sealing part 221 and the internal slope of the notch part 211 increase the sealing performance, so that the sealing part 221 and the notch part 211 of the main cover 21 can be completely sealed;

the clamping groove opening I212 and the clamping groove opening II 222 form a blade and stem through hole;

the outer ring vertical surface of the auxiliary cover 22 is provided with a screw thread II 224; the main cover 21 and the auxiliary cover 22 are matched into a whole, and the screw thread I2141 and the screw thread II 224 form a sealing cover sealing screw buckle of the upper cover 2;

the seal assembly 23 includes: the connecting rod 231, the sealing rubber gasket 232, the spring 233, the gasket 234, the snap spring 235 and the knob 236;

the connecting rod 231 is a multi-step revolving body; the lower end of the connecting rod 231 is a lower brim 2311, a first throat bar 2312 is arranged at the upper part of the lower brim 2311, a lower rod shaft 2313 is arranged at the upper part of the first throat bar 2312, and the diameter of the first throat bar 2312 is not more than that of the lower rod shaft 2313;

the lower rod shaft 2313 is provided with a main rod, the upper end of the main rod is provided with a second throat rod 2314, and the upper end of the second throat rod 2314 is provided with a clamping opening part 2315; a knob connecting part 2316 is arranged at the upper end of the bayonet part 2315;

the sealing rubber mat 232 is provided with a trepanning 2321 and a stem penetrating port 2322;

the sleeve hole 2321 is sleeved at the first throat bar 2312; the lower lever shaft 2313 is axially connected in the axial connection hole 223 of the auxiliary cover 22; the main rod of the connecting rod 231 is journaled in the shaft hole of the main cover 21; the washer 234 is sleeved at the second throat 2314; the clamp spring 235 is locked at the clamping opening portion 2315; the spring 233 is sleeved in the connecting rod 231, the lower contact surface of the spring 233 abuts against the upper end of the center of the main cover 21, and the upper contact surface of the spring 233 abuts against the lower contact surface of the gasket 234; the knob 236 is tightly coupled to the knob coupling portion 2316;

embodiment 2 a carbon isotope labeling method of portable plant leaves

The marking method of the embodiment adopts the carbon isotope marking device of the portable plant leaf in the embodiment 1;

in the marking process, the marking device needs to be placed in a place with better sunlight or on one side of a plant with better illumination; the general marking time is selected between 8:30 in the morning and 15:30 in the afternoon, so that the leaves of the plant to be marked can fully absorb the light for photosynthesis;

a carbon isotope labeling method of portable plant leaves comprises the following steps:

1) the upper cover 2 is taken down from the bottle body;

2) referring to fig. 5, pressing the knob 236 of the main cover 21, twisting the sub-cover 22 out of the notch 211 of the main cover 21;

3) sealing and winding the marked plant branches, leaves and stems by using a sealing binding band (the binding band can be used for plant grafting), so that the binding band, the slot I212 and the slot II 222 form a leaf-stem through hole which is tightly attached;

4) referring to FIG. 6, the leaf of the marked plant is downward, and one marked leaf or 2 leaves of the same leaf stem are selected; the leaf and stem are clamped in the leaf and stem through hole, the knob 236 on the main cover 21 is pressed, and the auxiliary cover 22 is twisted to be reset to the notch part 211 on the main cover 21; the main cover 21 and the auxiliary cover 22 are mutually closed inclined surfaces, and the close fit between the main cover 21 and the auxiliary cover 22 is increased under the lifting force of the spring 233;

5) placing the blade in the bottle body, and screwing the bottle cap 2;

6) after the leaves of the marked plants are placed in a bottle, firstly, the leaves of the plants are subjected to hunger treatment to reduce the gas content in the bottle;

7) a normal temperature nitrogen gas bag is connected through a first inflation nozzle 132 of the first inflation tube 13; the first inflation nozzle 142 of the second inflation tube 14 is accessed to a nominal temperature for labeling¹³CO₂A stable isotope air bag;

8) opening a first inflation control valve 131 of the first inflation tube 13, filling normal-temperature nitrogen into the bottle at a constant speed through manual extrusion or an air pump, and discharging other gases in the bottle through the one-way vent valve 12 by the nitrogen; the aeration time is 2 min;

9) the volume of the injected stable carbon isotope gas is 5ml during marking, and the marking time is controlled to be 30 min.

The technical scheme of the invention is explained in detail in the above with the accompanying drawings, and the invention provides a portable carbon isotope labeling device and a carbon isotope labeling method for plant leaves, wherein after the labeled plant leaves, leaves and stems are sealed and wound by a sealing bandage, the leaves, stems and stems through holes of an upper cover can be tightly attached; the gas in the bottle with plant leaves is flushed and exchanged with nitrogen, and after the flushing and exchanging are finished, the gas is marked with 13CO at normal temperature₂Filling to finish carbon isotope labeling; the method reduces the influence of factors such as gas and temperature in the bottle body during marking on the carbon isotope marking of the plant leaves, and improves the efficiency and reliability of carbon isotope stabilization of the plant leaves.