阅读说明：本技术 一种海藻液的提取方法 (Extraction method of seaweed liquid ) 是由 姚振领 于 2021-01-15 设计创作，主要内容包括：本发明涉及一种海藻液的提取方法,其特征在于,将海藻加入到水中,经酸解和酶解后,即得海藻液,所述酸解pH为2～3；所述酶是糖化酶和纤维素酶。本申请提取的海藻液水溶性好,固含量高。使用本方法对新鲜海藻提取,可以制备出固含量高的绿色海藻液。(The invention relates to a seaweed liquid extraction method which is characterized in that seaweed is added into water, and the seaweed liquid is obtained after acidolysis and enzymolysis, wherein the pH value of the acidolysis is 2-3; the enzyme is saccharifying enzyme and cellulase. The seaweed liquid extracted by the method is good in water solubility and high in solid content. The method can be used for extracting fresh seaweed to prepare green seaweed liquid with high solid content.)

1. The extraction method of the seaweed liquid is characterized by comprising the following steps:

1) acid hydrolysis: adding seaweed into water, and extracting under the condition that the pH value is 2-3 to obtain acidolysis solution;

2) enzymolysis: adjusting the pH value of the acidolysis solution to 4.2-6.5, adding enzyme, and carrying out enzymolysis to obtain an enzymolysis solution;

3) seaweed solution: the enzymolysis liquid obtained in the step 2) is seaweed liquid, or

Filtering the enzymolysis liquid to obtain filtrate, namely the seaweed liquid;

the enzyme is saccharifying enzyme and cellulase;

the mass ratio of the seaweed to the water in the step 1) is 1-2: 3-6;

the extraction conditions of the step 1) are 5-30 ℃, the stirring speed is 65-100 r/min, and the time is 20-40 min.

2. The method for extracting seaweed liquid according to claim 1, wherein the mass ratio of seaweed to enzyme is 10000: 0.1-5; the enzymolysis temperature is 26-62 ℃, and the enzymolysis time is 6-72 h.

3. The method for extracting seaweed liquid as set forth in claim 2, wherein the enzymatic hydrolysis is divided into saccharification enzymatic hydrolysis and cellulase enzymatic hydrolysis; the saccharification enzymolysis and the cellulase enzymolysis are not carried out simultaneously.

4. The method for extracting seaweed liquid according to claim 3, wherein the glycolytic enzymatic hydrolysis is carried out according to the following steps:

and adjusting the pH value of the acidolysis solution to 4.0-4.5, adding saccharifying enzyme, and carrying out enzymolysis to obtain a saccharifying enzymolysis seaweed solution.

5. The method for extracting seaweed liquid according to claim 3, wherein the cellulase enzymolysis is performed according to the following steps:

and adjusting the pH value of the acidolysis solution to 5.5-6.5, adding cellulase, and carrying out enzymolysis to obtain cellulase-hydrolyzed seaweed solution.

6. The method for extracting seaweed liquid according to claim 4, wherein the cellulase enzymolysis is performed according to the following steps:

adjusting the pH value of the saccharification and enzymolysis seaweed liquid to 5.5-6.5, adding cellulase, and carrying out enzymolysis to obtain cellulase-hydrolyzed seaweed liquid, namely the seaweed liquid; or

Filtering the cellulose hydrolysate to obtain seaweed liquid.

7. The method for extracting seaweed liquid according to claim 5, wherein the glycolytic enzymatic hydrolysis is carried out according to the following steps:

adjusting the pH value of the cellulase enzymolysis liquid to 4.0-4.5, adding saccharifying enzyme for enzymolysis to obtain a saccharifying enzymolysis seaweed liquid, namely the seaweed liquid; or

Filtering the saccharified enzymatic hydrolysate to obtain the seaweed liquid.

8. The method for extracting seaweed liquid as claimed in any one of claims 4, 5, 6 and 7, wherein the enzymolysis temperature of the saccharification enzyme enzymolysis is 55-62 ℃, and the enzymolysis time is 6-24 h; enzyme deactivation is carried out after enzymolysis, wherein the enzyme deactivation temperature is 90-120 ℃, and the enzyme deactivation time is 2-5 min;

the enzymolysis temperature of the cellulase enzymolysis is 26-35 ℃, and the enzymolysis time is 6-48 h; and enzyme deactivation is carried out after enzymolysis, wherein the enzyme deactivation temperature is 90-120 ℃, and the enzyme deactivation time is 2-5 min.

9. The method for extracting seaweed liquid as claimed in any one of claims 4, 5, 6 and 7, wherein the enzymatic hydrolysis with cellulase further comprises introducing nitrogen gas before the enzymatic hydrolysis until the oxygen content in the saccharified enzymatic hydrolysate or the acidolyzed solution is less than 0.2 mg/L;

the saccharification enzymatic hydrolysis also comprises introducing nitrogen before the enzymatic hydrolysis until the oxygen content in the saccharification enzymatic hydrolysis liquid or the acidolysis liquid is lower than 0.2 mg/L.

10. The method for extracting seaweed liquid as claimed in claim 8, wherein the enzymatic hydrolysis with cellulase further comprises introducing nitrogen gas before the enzymatic hydrolysis until the oxygen content in the saccharified enzymatic hydrolysate or acidolyzed solution is less than 0.2 mg/L;

the saccharification enzymatic hydrolysis also comprises introducing nitrogen before the enzymatic hydrolysis until the oxygen content in the saccharification enzymatic hydrolysis liquid or the acidolysis liquid is lower than 0.2 mg/L.

Technical Field

The invention relates to a method for extracting seaweed liquid.

Background

At present, the method for chemically extracting the seaweed liquid is divided into an alkaline hydrolysis extraction mode, an acidolysis extraction mode or a combination extraction mode of the alkaline hydrolysis extraction mode and the acidolysis extraction mode, the prepared extracting solution is dark brown or black, the color is deepened along with the extension of the extraction time, the fragrance is emitted, and finally the solid content of water-soluble products in the extracting solution is low, so that part of effective substances are lost. The direct extraction of acid at low temperature can reduce the damage to effective components, but the seaweed gel is generated, the product fluidity is poor, the subsequent operation is not facilitated, the inconvenience is brought to producers and users, and the problem of low water-soluble solid content exists. In order to prevent the product from deteriorating in fluidity after acidolysis and facilitate production and use, alkali extraction is generally adopted first, and then acid extraction is adopted, however, due to the damage of the alkali extraction on the effective components of the seaweed, the prepared product is black even if acid extraction is adopted in the later period, and the water-soluble solid content is also low. For example, patent No. CN 1269339a discloses a technical scheme of alkaline hydrolysis followed by acid hydrolysis.

At present, no method exists for extracting by acid, which does not destroy the effective components of the seaweed, can ensure that the product has good fluidity, can further improve the solid content of the extracting solution and can ensure the stable quality of the finally prepared product.

Disclosure of Invention

The invention provides an extraction method of seaweed liquid, which solves the technical problems that 1) the damage to the effective ingredients of seaweed is reduced, and the product has good fluidity; 2) further improving the water-soluble solid content in the seaweed extract and stabilizing the quality of the extracted seaweed liquid.

In order to solve the technical problems, the invention adopts the following technical scheme:

the extraction method of the seaweed liquid comprises the following steps:

1) acid hydrolysis: adding seaweed into water, and extracting under the condition that the pH value is 2-3 to obtain acidolysis solution;

2) enzymolysis: adjusting the pH value of the acidolysis solution to 4.2-6.5, adding enzyme, and carrying out enzymolysis;

3) seaweed solution: the enzymolysis liquid obtained in the step 2) is seaweed liquid, or

Filtering the enzymolysis liquid to obtain filtrate, namely the seaweed liquid;

the enzyme is saccharifying enzyme and cellulase.

The mass ratio of the seaweed to the water in the step 1) is 1-2: 3-6.

The extraction conditions of the step 1) are 5-30 ℃, the rotating speed is 65-100 r/min, and the time is 20-40 min.

The enzymolysis is divided into saccharification enzymolysis and cellulase enzymolysis; the mass ratio of the seaweed to the enzyme is 10000: 0.1-5; the enzymolysis temperature is 26-62 ℃, and the enzymolysis time is 6-72 hours; the saccharification enzymolysis and the cellulase enzymolysis are not carried out simultaneously.

The saccharification enzymatic hydrolysis is carried out according to the following steps:

and adjusting the pH value of the acidolysis solution to 4.0-4.5, adding saccharifying enzyme, and carrying out enzymolysis to obtain a saccharifying enzymolysis seaweed solution.

The cellulase enzymolysis is carried out according to the following steps:

and adjusting the pH value of the acidolysis solution to 5.5-6.5, adding cellulase, and carrying out enzymolysis to obtain cellulase-hydrolyzed seaweed solution.

The cellulase enzymolysis is carried out according to the following steps:

adjusting the pH value of the saccharification enzymolysis liquid to 5.5-6.5, adding cellulase, and carrying out enzymolysis to obtain cellulase-hydrolyzed seaweed liquid, namely the seaweed liquid; or

Filtering the cellulose hydrolysate to obtain seaweed liquid.

The saccharification enzymatic hydrolysis is carried out according to the following steps:

adjusting the pH value of the cellulase enzymolysis liquid to 4.0-4.5, adding saccharifying enzyme for enzymolysis to obtain a saccharifying enzymolysis seaweed liquid, namely the seaweed liquid; or

Filtering the saccharified enzymatic hydrolysate to obtain the seaweed liquid.

The enzymolysis temperature of the saccharification enzymatic hydrolysis is 55-62 ℃, and the enzymolysis time is 6-24 h; enzyme deactivation is carried out after enzymolysis, wherein the enzyme deactivation temperature is 90-120 ℃, and the enzyme deactivation time is 2-5 min;

the enzymolysis temperature of the cellulase enzymolysis is 26-35 ℃, and the enzymolysis time is 6-48 h; and enzyme deactivation is carried out after enzymolysis, wherein the enzyme deactivation temperature is 90-120 ℃, and the enzyme deactivation time is 2-5 min.

The enzymolysis of the cellulase also comprises introducing nitrogen before the enzymolysis until the oxygen content in the saccharified enzymolysis liquid or the acidolysis liquid is lower than 0.2 mg/L;

the saccharification enzymatic hydrolysis also comprises introducing nitrogen before the enzymatic hydrolysis until the oxygen content in the saccharification enzymatic hydrolysis liquid or the acidolysis liquid is lower than 0.2 mg/L.

The invention has the following beneficial technical effects:

1. this application reduces the destruction of acid to the interior material of marine alga through low temperature acidolysis, simultaneously, through earlier stage acid treatment, obtains the acidolysis liquid, through later stage enzyme extraction, both can reduce the destruction to the marine alga composition, can make the extract have better mobility again, can also further improve water-soluble solid content in the marine alga extract.

2. According to the method, the seaweed is subjected to enzymolysis by adopting saccharifying enzyme and cellulase, and the enzymolysis is performed step by step, so that the water-soluble solid content in the seaweed liquid can be improved, wherein the saccharifying enzyme is used for hydrolyzing alpha-1, 4-and alpha-1, 6-glycosidic bonds, the cellulase is used for hydrolyzing 1, 4-beta glycosidic bonds, and the extraction rate of the seaweed polysaccharide can be improved under the combined action of the saccharifying enzyme and the cellulase.

3. This application can effectively prevent extract colour change, makes product quality more stable.

Detailed Description

The present invention is further illustrated by the following specific examples.

Example 1

The extraction method of the seaweed liquid comprises the following steps:

1) acid hydrolysis: adding seaweed into water, and extracting under the condition that the pH value is 2.2 to obtain acidolysis solution;

2) enzymolysis: adjusting the pH value of the acidolysis solution to 6.0, adding enzyme, and carrying out enzymolysis to obtain an enzymolysis solution;

3) seaweed solution: inactivating enzyme of the enzymolysis solution at 95 deg.C for 3min, and filtering to obtain filtrate, i.e. Sargassum solution.

The enzyme is a mixture of saccharifying enzyme and cellulase according to the mass ratio of 1: 1; the seaweed is fresh Sargassum, and has water content of 80%.

The mass ratio of the seaweed and the water in the step 1) is 1: 3.

The extraction conditions of the step 1) are that the temperature is 15 ℃, the rotating speed is 65r/min, and the time is 30 min.

The mass ratio of the added enzyme to the kelp in the step 2) is 0.4:10000, wherein the activity of saccharifying enzyme is 50000U/g, the activity of cellulase is 100000U/g, the enzymolysis condition is that the enzymolysis temperature is 30 ℃, and the enzymolysis time is 24 hours.

And 3) filtering, wherein the mesh diameter of the filter screen is 400 meshes.

The steps relate to the seaweed mass ratio on a dry basis.

Example 2

The extraction method of the seaweed liquid comprises the following steps:

1) acid hydrolysis: adding seaweed into water, and extracting under the condition that the pH value is 2.2 to obtain acidolysis solution;

2) enzymolysis: adjusting the pH value of the acidolysis solution to 4.2, adding enzyme, and carrying out enzymolysis to obtain an enzymolysis solution;

3) seaweed solution: inactivating enzyme of the enzymolysis solution at 95 deg.C for 3min, and filtering to obtain filtrate, i.e. Sargassum solution.

The enzyme is a mixture of saccharifying enzyme and cellulase according to the mass ratio of 1: 1; the seaweed is fresh Sargassum, and has water content of 80%.

The mass ratio of the seaweed and the water in the step 1) is 1: 3.

The extraction conditions of the step 1) are that the temperature is 15 ℃, the rotating speed is 65r/min, and the time is 30 min.

The mass ratio of the added enzyme to the kelp in the step 2) is 0.4:10000, wherein the activity of saccharifying enzyme is 50000U/g, the activity of cellulase is 100000U/g, the enzymolysis condition is that the enzymolysis temperature is 60 ℃, and the enzymolysis time is 24 hours.

And 3) filtering, wherein the mesh diameter of the filter screen is 400 meshes.

The steps relate to the seaweed mass ratio on a dry basis.

Example 3

The extraction method of the seaweed liquid comprises the following steps:

1) acid hydrolysis: adding seaweed into water, and extracting under the condition that the pH value is 2.2 to obtain acidolysis solution;

2) enzymolysis: adjusting pH of the acidolysis solution to 4.2, adding diastase, performing enzymolysis, and inactivating enzyme to obtain saccharified and enzymolyzed seaweed solution;

adjusting the pH value of the saccharification and enzymolysis seaweed solution to 6.0, adding cellulase for enzymolysis to obtain cellulase enzymolysis seaweed solution;

3) seaweed solution: inactivating enzyme of the cellulose enzymolysis Sargassum liquid at 95 deg.C for 3min, and filtering to obtain filtrate.

The mass ratio of the seaweed to the water in the step 1) is 1: 3; the seaweed is fresh Sargassum, and has water content of 80%.

The mass ratio of the seaweed and the water in the step 1) is 1: 3.

The extraction conditions of the step 1) are that the temperature is 15 ℃, the rotating speed is 65r/min, and the time is 30 min.

In the step 2), the mass ratio of the adding amount of the saccharifying enzyme to the kelp is 0.2:10000, the mass ratio of the adding amount of the cellulase to the kelp is 0.2:10000, wherein the activity of the saccharifying enzyme is 50000U/g, the activity of the cellulase is 100000U/g,

in the step 2), the enzymatic hydrolysis conditions of the saccharification enzyme are that the enzymatic hydrolysis temperature is 60 ℃ and the enzymatic hydrolysis time is 12 hours;

the enzymolysis condition of the cellulase is that the enzymolysis temperature is 30 ℃, and the enzymolysis time is 12 h.

And 3) filtering, wherein the mesh diameter of the filter screen is 400 meshes.

The steps relate to the seaweed mass ratio on a dry basis.

Example 4

The extraction method of the seaweed liquid comprises the following steps:

1) acid hydrolysis: adding seaweed into water, and extracting under the condition that the pH value is 2.2 to obtain acidolysis solution;

2) enzymolysis: adjusting pH of the acidolysis solution to 6.0, adding cellulase for enzymolysis, and inactivating enzyme to obtain cellulase-hydrolyzed seaweed solution;

adjusting the pH value of the cellulase-hydrolyzed seaweed solution to 4.2, adding saccharifying enzyme, and performing enzymolysis to obtain saccharified and enzymolyzed seaweed solution;

3) seaweed solution: inactivating enzyme of saccharified and enzymolyzed Sargassum liquid at 95 deg.C for 3min, and filtering to obtain filtrate.

The mass ratio of the seaweed to the water in the step 1) is 1: 3; the seaweed is fresh Sargassum, and has water content of 80%.

The mass ratio of the seaweed and the water in the step 1) is 1: 3.

The extraction conditions of the step 1) are that the temperature is 15 ℃, the rotating speed is 65r/min, and the time is 30 min.

In the step 2), the mass ratio of the adding amount of the saccharifying enzyme to the kelp is 0.2:10000, the mass ratio of the adding amount of the cellulase to the kelp is 0.2:10000, wherein the activity of the saccharifying enzyme is 50000U/g, the activity of the cellulase is 100000U/g,

in the step 2), the enzymatic hydrolysis conditions of the saccharification enzyme are that the enzymatic hydrolysis temperature is 60 ℃ and the enzymatic hydrolysis time is 12 hours;

the enzymolysis condition of the cellulase is that the enzymolysis temperature is 30 ℃, and the enzymolysis time is 12 h.

And 3) filtering, wherein the mesh diameter of the filter screen is 400 meshes.

The steps relate to the seaweed mass ratio on a dry basis.

Example 5

The extraction method of the seaweed liquid comprises the following steps:

1) acid hydrolysis: adding seaweed into water, and extracting under the condition that the pH value is 2.2 to obtain acidolysis solution;

2) enzymolysis: adjusting the pH value of the acidolysis solution to 4.2, charging nitrogen until the oxygen content in the acidolysis solution is 0.1mg/L, adding saccharifying enzyme, carrying out enzymolysis under the protection of nitrogen, and inactivating enzyme to obtain saccharified and enzymolyzed seaweed liquid;

adjusting the pH value of the saccharification and enzymolysis seaweed solution to 6.0, adding cellulase, and carrying out enzymolysis under the protection of nitrogen to obtain cellulase enzymolysis seaweed solution;

3) seaweed solution: inactivating enzyme of the cellulose enzymolysis Sargassum liquid at 95 deg.C for 3min, and filtering to obtain filtrate.

The mass ratio of the seaweed to the water in the step 1) is 1: 3; the seaweed is fresh Sargassum, and has water content of 80%.

The mass ratio of the seaweed and the water in the step 1) is 1: 3.

The extraction conditions of the step 1) are that the temperature is 15 ℃, the rotating speed is 65r/min, and the time is 30 min.

In the step 2), the mass ratio of the adding amount of the saccharifying enzyme to the kelp is 0.2:10000, the mass ratio of the adding amount of the cellulase to the kelp is 0.2:10000, wherein the activity of the saccharifying enzyme is 50000U/g, the activity of the cellulase is 100000U/g,

in the step 2), the enzymatic hydrolysis conditions of the saccharification enzyme are that the enzymatic hydrolysis temperature is 60 ℃ and the enzymatic hydrolysis time is 12 hours;

the enzymolysis condition of the cellulase is that the enzymolysis temperature is 30 ℃, and the enzymolysis time is 12 h.

And 3) filtering, wherein the mesh diameter of the filter screen is 400 meshes.

The steps relate to the seaweed mass ratio on a dry basis.

Example 6

The extraction method of the seaweed liquid comprises the following steps:

1) acid hydrolysis: adding seaweed into water, and extracting under the condition that the pH value is 2.2 to obtain acidolysis solution;

2) enzymolysis: adjusting the pH value of the acidolysis solution to 6.0, charging nitrogen until the oxygen content in the acidolysis solution is 0.1mg/L, adding cellulase, performing enzymolysis under the protection of nitrogen, and inactivating enzyme to obtain cellulase-hydrolyzed seaweed solution;

adjusting the pH value of the cellulase-hydrolyzed seaweed solution to 4.2, adding saccharifying enzyme, and carrying out enzymolysis under the protection of nitrogen to obtain a saccharified and enzymolyzed seaweed solution;

3) seaweed solution: inactivating enzyme of saccharified and enzymolyzed Sargassum liquid at 95 deg.C for 3min, and filtering to obtain filtrate.

The mass ratio of the seaweed to the water in the step 1) is 1: 3; the seaweed is fresh Sargassum, and has water content of 80%.

The mass ratio of the seaweed and the water in the step 1) is 1: 3.

The extraction conditions of the step 1) are that the temperature is 15 ℃, the rotating speed is 65r/min, and the time is 30 min.

In the step 2), the mass ratio of the adding amount of the saccharifying enzyme to the kelp is 0.2:10000, the mass ratio of the adding amount of the cellulase to the kelp is 0.2:10000, wherein the activity of the saccharifying enzyme is 50000U/g, the activity of the cellulase is 100000U/g,

in the step 2), the enzymatic hydrolysis conditions of the saccharification enzyme are that the enzymatic hydrolysis temperature is 60 ℃ and the enzymatic hydrolysis time is 12 hours;

the enzymolysis condition of the cellulase is that the enzymolysis temperature is 30 ℃, and the enzymolysis time is 12 h.

And 3) filtering, wherein the mesh diameter of the filter screen is 400 meshes.

The steps relate to the seaweed mass ratio on a dry basis.

Example 7

The extraction method of the seaweed liquid comprises the following steps:

1) acid hydrolysis: adding seaweed into water, and extracting under the condition that the pH value is 2.5 to obtain acidolysis solution;

2) enzymolysis: adjusting pH of the acidolysis solution to 5.8, adding cellulase for enzymolysis, and inactivating enzyme to obtain cellulase-hydrolyzed seaweed solution;

adjusting the pH value of the cellulase-hydrolyzed seaweed solution to 4.4, charging nitrogen until the oxygen content in the acidolysis solution is 0.05mg/L, adding saccharifying enzyme, and performing enzymolysis to obtain saccharified and enzymolyzed seaweed solution;

3) seaweed solution: inactivating enzyme of saccharified and enzymolyzed Sargassum liquid at 100 deg.C for 2min, and filtering to obtain filtrate.

The mass ratio of the seaweed to the water in the step 1) is 1: 4; the seaweed is thallus laminariae.

The extraction conditions of the step 1) are that the temperature is 10 ℃, the rotating speed is 80r/min, and the time is 35 min.

In the step 2), the mass ratio of the adding amount of the saccharifying enzyme to the kelp is 0.1:10000, the mass ratio of the adding amount of the cellulase to the kelp is 0.3:10000, wherein the activity of the saccharifying enzyme is 100000U/g, the activity of the cellulase is 50000U/g,

in the step 2), the enzymatic hydrolysis conditions of the saccharification enzyme are that the enzymatic hydrolysis temperature is 56 ℃ and the enzymatic hydrolysis time is 8 hours;

the enzymolysis condition of the cellulase is that the enzymolysis temperature is 28 ℃, and the enzymolysis time is 24 hours.

Example 8

The extraction method of the seaweed liquid comprises the following steps:

1) acid hydrolysis: adding seaweed into water, and extracting under the condition that the pH value is 2.8 to obtain acidolysis solution;

2) enzymolysis: adjusting the pH value of the acidolysis solution to 4.5, charging nitrogen until the oxygen content in the acidolysis solution is 0.1mg/L, adding saccharifying enzyme, carrying out enzymolysis under the protection of nitrogen, and inactivating enzyme to obtain saccharified and enzymolyzed seaweed liquid;

adjusting the pH value of the saccharification and enzymolysis seaweed solution to 6.2, adding cellulase for enzymolysis to obtain cellulase enzymolysis seaweed solution;

3) seaweed solution: inactivating enzyme of the cellulose enzymolysis Sargassum liquid at 100 deg.C for 2min, and filtering to obtain filtrate.

Step 1), the mass ratio of the seaweed to the water is 1: 2; the seaweed is laver.

The extraction conditions of the step 1) are that the temperature is 25 ℃, the rotating speed is 65r/min, and the time is 20 min.

In the step 2), the mass ratio of the adding amount of the saccharifying enzyme to the kelp is 0.5:10000, the mass ratio of the adding amount of the cellulase to the kelp is 0.6:10000, wherein the activity of the saccharifying enzyme is 50000U/g, the activity of the cellulase is 50000U/g,

in the step 2), the enzymatic hydrolysis conditions of the saccharification enzyme are that the enzymatic hydrolysis temperature is 56 ℃ and the enzymatic hydrolysis time is 24 hours;

the enzymolysis condition of the cellulase is that the enzymolysis temperature is 32 ℃, and the enzymolysis time is 24 hours.

The beneficial effects of the present invention are further illustrated below in conjunction with experimental data:

test material

1.1 test site: the detection was made by Sanfeng (tobacco pipe) agriculture science and technology Co.

1.2 test detection: the method comprises the following steps of (1) recording the content of water-soluble solid content, the content of initial cytokinin (the general names of isopentenyladenine, zeatin, isopentenyladenosine, trans-zeatin and gibberellin), the content of final cytokinin (the general names of isopentenyladenine, zeatin, isopentenyladenosine, trans-zeatin and gibberellin), the content of initial algal polysaccharide and the content of final algal polysaccharide, and recording the initial color and the final color, wherein the initial color is the color at the moment of production, and the content of initial algal polysaccharide is the color at the moment of production; the content of inceptocytokinins (a general term for isopentenyladenine, zeatin, isopentenyladenosine, trans-zeatin and gibberellin) is as-produced; the final color is the color after being placed indoors in a 1L polyethylene bottle for 6 months, and the final cytokinin content (the general name of isopentene adenine, zeatin, isopentene adenosine, trans-zeatin and gibberellin) is the cytokinin content (the general name of isopentene adenine, zeatin, isopentene adenosine, trans-zeatin and gibberellin) after being placed indoors in a 1L polyethylene bottle for 6 months; the final algal polysaccharide content is the algal polysaccharide content after being placed indoors for 6 months in a 1L polyethylene bottle.

1.3 test materials: blank (acidolysis solution prepared in example 2), comparative example 1 (except that no acidolysis was performed, the other preparation methods were the same as in example 3), comparative example 2 (except that only cellulase was used as enzyme for enzymatic hydrolysis, the other preparation methods were the same as in example 1), comparative example 3 (except that only glucoamylase was used as enzyme for enzymatic hydrolysis, the other preparation methods were the same as in example 2), and examples 1 to 6.

1.4 Experimental implementation: the solid content is determined according to the coating solid content determination method GB1725-79, the seaweed polysaccharide is detected by a sulfuric acid-phenol method, the cytokinin is detected by a liquid chromatography-mass spectrometry combined method, and the color change is observed by naked eyes.

The present application is consistent in other implementations except for differences in the respective processes.

1.5 Experimental phenomena: wherein the blank (acidolysis solution prepared in example 2) was seaweed gel, losing fluidity; the fluidity is restored after the enzyme treatment, and the fluidity is increased with the increase of the water-soluble solid content.

2 results and analysis

Water-soluble solid content, cytokinin content (a total name of isopentenyladenine, zeatin, isopentenyladenosine, trans-zeatin and gibberellin), final cytokinin content (a total name of isopentenyladenine, zeatin, isopentenyladenosine, trans-zeatin and gibberellin), algal polysaccharide content and algal polysaccharide content, and initial color and final color are shown in Table 1

TABLE 1

Note: the blank (acidolysis solution prepared in example 2) was prepared as alginate gel, which was not water-soluble, too viscous to filter and therefore not tested for solid content.

As can be seen from comparison of the blank in Table 1 (acidolysis solution prepared in example 2) and the data in example 2, the flowability of the product can be improved after the acidolysis solution is subjected to enzymolysis, and the solid content and algal polysaccharide content in the extract can be improved;

as can be seen from comparison of data between comparative example 2 (except that only cellulase is used as enzyme in the enzymatic hydrolysis, the other preparation methods are consistent with those of example 1) and example 1 in table 1, and comparative example 3 (except that only glucoamylase is used as enzyme in the enzymatic hydrolysis, the other preparation methods are consistent with those of example 2) and example 2, the solid content and polysaccharide content in the seaweed liquid can be remarkably improved by using the combined extraction of the glucoamylase and the cellulase; as can be seen from comparison of the data in Table 1 for comparison 1 (except that no acidolysis, the other preparation methods are the same as those in example 3) and example 3, the solid content of example 3 extracted by the combination of acidolysis and enzymolysis is increased by 12%, the algal polysaccharide is increased by 4.4%, and the cytokinin is reduced by 0.6. mu.g/g, compared with comparative example 1 only subjected to enzymolysis. As can be seen from the comparison of the data of the embodiment 1, the embodiment 2, the embodiment 3 and the embodiment 4, the effect of the embodiment 3 and the embodiment 4 which respectively extract by adopting the saccharifying enzyme and the cellulase is obviously better than that of the embodiment 1 and the embodiment 2 which adopt the complex enzyme; as can be seen from the comparison of the data in the examples 3, 4, 5 and 6, the stability of the quality of the seaweed liquid can be obviously improved by introducing nitrogen for protection before enzymolysis.