阅读说明：本技术 一种具有促进作物生根功能的生物有机肥及其制备方法 (Bio-organic fertilizer with crop rooting promoting function and preparation method thereof ) 是由 李凤兰 冯哲 李柱刚 张世宏 冯艳忠 何流琴 吴建 杨秀梅 梁飞 田文会 常婷婷 于 2020-11-02 设计创作，主要内容包括：本发明公开了一种具有促进作物生根功能的生物有机肥及其制备方法,该肥料由动物粪便、油糠、糖蜜、磷酸二氢钙和复合益生菌液组成的原料制备而成。原料中各组分以重量计为：动物粪便60-70份、油糠21-27份、糖蜜2-5份、磷酸二氢钙7-10份和复合益生菌液0.1-0.2份。该方法包括以下步骤：将枯草芽孢杆菌、植物乳杆菌和酿酒酵母混合制备复合益生菌液；将各原料组分混合均匀得到混合物料,加水调节其含水量；物料初始pH自然,在高于4℃的温度下兼氧发酵30天以上,当发酵物料中的粪大肠菌群数≤20时结束发酵,获得生物有机肥。本发明能将动物粪便转化为有机肥料,减轻环境污染,施用本发明可促进作物生长,提高作物产量。(The invention discloses a biological organic fertilizer with a function of promoting crop rooting and a preparation method thereof. The raw materials comprise the following components in parts by weight: 60-70 parts of animal waste, 21-27 parts of oil bran, 2-5 parts of molasses, 7-10 parts of calcium dihydrogen phosphate and 0.1-0.2 part of composite probiotic liquid. The method comprises the following steps: mixing bacillus subtilis, lactobacillus plantarum and saccharomyces cerevisiae to prepare composite probiotic liquid; uniformly mixing the raw material components to obtain a mixed material, and adding water to adjust the water content of the mixed material; the initial pH of the material is natural, the fermentation is carried out for more than 30 days at the temperature of higher than 4 ℃, and the fermentation is finished when the number of faecal coliform bacteria in the fermented material is less than or equal to 20, so as to obtain the biological organic fertilizer. The invention can convert animal waste into organic fertilizer, reduce environmental pollution, promote crop growth and increase crop yield.)

1. A biological organic fertilizer with the function of promoting crop rooting is prepared from raw materials consisting of animal wastes, oil bran, molasses, calcium dihydrogen phosphate and compound probiotic liquid.

2. The bio-organic fertilizer according to claim 1, characterized in that: the biological organic fertilizer is prepared by uniformly mixing the raw materials, adjusting the water content to be 20-85%, and then carrying out facultative fermentation at the temperature higher than 4 ℃ for more than 30 days, wherein the number of faecal coliform groups in the biological organic fertilizer is less than or equal to 20.

3. The bio-organic fertilizer according to claim 1, characterized in that: the raw materials comprise, by weight, 60-70 parts of the animal waste, 21-27 parts of the oil bran, 2-5 parts of molasses, 7-10 parts of monocalcium phosphate and 0.1-0.2 part of the composite probiotic liquid.

4. The bio-organic fertilizer according to claim 3, characterized in that: the raw materials comprise, by weight, 60 parts of the animal waste, 27 parts of the oil bran, 2 parts of the molasses, 8 parts of the monocalcium phosphate and 0.1 part of the composite probiotic liquid.

5. The bio-organic fertilizer according to claim 3, characterized in that: the raw materials comprise, by weight, 64 parts of animal wastes, 25 parts of oil bran, 4 parts of molasses, 10 parts of monocalcium phosphate and 0.13 part of composite probiotic liquid.

6. The bio-organic fertilizer according to claim 3, characterized in that: the raw materials comprise 67 parts of animal waste, 23 parts of oil bran, 3 parts of molasses, 7 parts of monocalcium phosphate and 0.17 part of composite probiotic liquid by weight.

7. The bio-organic fertilizer according to claim 3, characterized in that: the raw materials comprise, by weight, 70 parts of the animal waste, 21 parts of the oil bran, 5 parts of the molasses, 9 parts of the monocalcium phosphate and 0.2 part of the composite probiotic liquid.

8. Bio-organic fertilizer according to any one of claims 1 to 7, characterized in that: the composite probiotic liquid comprises bacillus subtilis, lactobacillus plantarum and saccharomyces cerevisiae.

9. Root of herbaceous plantThe bio-organic fertilizer according to claim 8, characterized in that: the bacteria content of the bacillus subtilis is more than or equal to 1.0 multiplied by 10¹⁰cfu/mL, the bacterium content of the lactobacillus plantarum is more than or equal to 4.0 multiplied by 10¹¹cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 5.0 multiplied by 10⁸cfu/mL。

10. A preparation method of a biological organic fertilizer with the function of promoting crop rooting is characterized by comprising the following steps: the preparation method comprises the following steps:

step 1, preparing composite probiotic liquid, and mixing bacillus subtilis liquid, lactobacillus plantarum liquid and saccharomyces cerevisiae liquid;

step 2, preparing a mixed material, uniformly mixing animal wastes, oil bran, molasses, monocalcium phosphate and the composite probiotic liquid to obtain the mixed material, and adding water to adjust the water content of the mixed material;

and 3, fermenting, wherein the initial pH of the material is natural, performing facultative fermentation for more than 30 days at the temperature higher than 4 ℃, finishing fermentation when the number of faecal coliform bacteria in the fermented material is less than or equal to 20, and taking the fermented product as the bio-organic fertilizer.

Technical Field

The invention belongs to the technical field of fertilizers, and particularly relates to a biological organic fertilizer with a function of promoting crop rooting and a preparation method thereof.

Background

In recent years, the rapid development of animal breeding industry provides abundant animal products for people, but animals can generate a large amount of wastes such as excrement and urine in the growth process, if the wastes cannot be recycled in time, the problem of serious environmental pollution can be caused, and the sustainable development of social economy is not facilitated.

In addition, with the rapid development of the planting industry, farmers reduce the application of organic fertilizers and apply a large amount of fertilizers to farmlands in order to pursue the improvement of yield and short-term economic benefits. However, long-term unreasonable fertilization has destroyed the structure and ecosystem of the soil, resulting in the decrease of soil fertility and serious influence on crop production.

Therefore, the animal manure is converted into the organic fertilizer required by the growth of crops, and the organic fertilizer has great significance for resource utilization of the animal manure and improvement of soil fertility.

Disclosure of Invention

The invention aims to solve the technical problem of providing a biological organic fertilizer with the function of promoting crop rooting and a preparation method thereof, which convert animal wastes into an organic fertilizer through fermentation, realize the recycling treatment of the animal wastes and reduce the environmental pollution.

On one hand, the invention provides a biological organic fertilizer with a function of promoting crop rooting, which is prepared from raw materials consisting of animal wastes, oil bran, molasses, monocalcium phosphate and compound probiotic liquid.

Preferably, the bio-organic fertilizer is prepared by uniformly mixing the raw materials, adjusting the water content to be 20-85%, and then carrying out facultative fermentation at the temperature higher than 4 ℃ for more than 30 days, wherein the number of faecal coliform bacteria in the bio-organic fertilizer is less than or equal to 20.

Preferably, the raw materials comprise, by weight, 60-70 parts of the animal wastes, 21-27 parts of the oil bran, 2-5 parts of the molasses, 7-10 parts of the monocalcium phosphate and 0.1-0.2 part of the compound probiotic liquid.

Preferably, the raw materials comprise, by weight, 60 parts of the animal manure, 27 parts of the oil bran, 2 parts of the molasses, 8 parts of the monocalcium phosphate and 0.1 part of the compound probiotic liquid.

Preferably, the raw materials comprise 64 parts of the animal manure, 25 parts of the oil bran, 4 parts of the molasses, 10 parts of the monocalcium phosphate and 0.13 part of the compound probiotic liquid by weight.

Preferably, the raw materials comprise 67 parts of the animal manure, 23 parts of the oil bran, 3 parts of the molasses, 7 parts of the monocalcium phosphate and 0.17 part of the compound probiotic liquid by weight.

Preferably, the raw materials comprise 70 parts of the animal manure, 21 parts of the oil bran, 5 parts of the molasses, 9 parts of the monocalcium phosphate and 0.2 part of the compound probiotic liquid by weight.

Preferably, the composite probiotic liquid comprises bacillus subtilis, lactobacillus plantarum and saccharomyces cerevisiae.

Preferably, the bacterium content of the bacillus subtilis is more than or equal to 1.0 multiplied by 10¹⁰cfu/mL, the bacterium content of the lactobacillus plantarum is more than or equal to 4.0 multiplied by 10¹¹cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 5.0 multiplied by 10⁸cfu/mL。

On the other hand, the invention also provides a preparation method of the biological organic fertilizer with the function of promoting crop rooting, which comprises the following steps:

step 1, preparing composite probiotic liquid, and mixing bacillus subtilis liquid, lactobacillus plantarum liquid and saccharomyces cerevisiae liquid;

step 2, preparing a mixed material, uniformly mixing animal wastes, oil bran, molasses, monocalcium phosphate and the composite probiotic liquid to obtain the mixed material, and adding water to adjust the water content of the mixed material;

and 3, fermenting, wherein the initial pH of the material is natural, performing facultative fermentation for more than 30 days at the temperature higher than 4 ℃, finishing fermentation when the number of faecal coliform bacteria in the fermented material is less than or equal to 20, and taking the fermented product as the bio-organic fertilizer.

The biological organic fertilizer with the function of promoting the rooting of the crops can convert animal wastes into the organic fertilizer through fermentation, realize the recycling of the animal wastes, reduce the environmental pollution, promote the growth of the crops and improve the yield of the crops by applying the biological organic fertilizer.

Detailed Description

The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. It should be understood that the examples are illustrative only and are not limiting upon the scope of the invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.

In the following description, all methods involved are conventional in the art unless otherwise specified. The starting materials mentioned are all those which are commercially available from the public unless otherwise specified.

The invention mixes the animal waste with oil bran, molasses and calcium dihydrogen phosphate, and then adopts probiotics fermentation to prepare the biological organic fertilizer with the function of promoting crop rooting (for the convenience of expression, the biological organic fertilizer is abbreviated as biological organic fertilizer in the following). The animal waste is converted into the organic fertilizer through fermentation, so that the recycling of the animal waste is realized, the environmental pollution is reduced, the growth of crops can be promoted by applying the organic fertilizer, and the yield of the crops is increased.

In a specific embodiment of the invention, the bio-organic fertilizer is prepared from raw materials consisting of animal wastes, oil bran, molasses, calcium dihydrogen phosphate and compound probiotic liquid. The specific preparation process comprises the following steps: step 1, preparing composite probiotic liquid; step 2, preparing a mixed material; and 3, fermenting the mixed material.

In the step 1 of preparing the composite probiotic liquid, Bacillus subtilis liquid, Lactobacillus plantarum liquid and Saccharomyces cerevisiae liquid are selected, and then the three kinds of liquid are compounded according to the proportion by weight to obtain the composite probiotic liquid, preferably 20-35 parts of the Bacillus subtilis liquid, 30-40 parts of the Lactobacillus plantarum liquid and 20-30 parts of the Saccharomyces cerevisiae liquid. Wherein the bacterium content of the bacillus subtilis liquid is more than or equal to 1.0 multiplied by 10¹⁰cfu/mL, the bacterial content of the lactobacillus plantarum bacterial liquid is more than or equal to 4.0 multiplied by 10¹¹cfu/mL, the bacterium content of the saccharomyces cerevisiae liquid is more than or equal to 5.0 multiplied by 10⁸cfu/mL. The three kinds of bacterial liquids can be prepared by self, and bacillus subtilis, lactobacillus plantarum and saccharomyces cerevisiae are respectively subjected to high-density fermentation culture in corresponding liquid culture media to obtain the bacterial liquids.

In the step 2 of preparing the mixed material, various raw material components are weighed according to a preset weight ratio, then all the raw materials are mixed and stirred, and meanwhile, clear water is added into the mixture to adjust the water content of the mixed material. The weight ratio of each component in the raw materials is respectively optimized as follows: 60-70 parts of animal waste, 21-27 parts of oil bran, 2-5 parts of molasses, 7-10 parts of calcium dihydrogen phosphate and 0.1-0.2 part of composite probiotic liquid, wherein the water content of the mixed materials is preferably 20-85%.

And 3, fermenting the mixed material, namely fermenting the mixed material with natural initial pH for more than 30 days at the temperature higher than 4 ℃ by facultative fermentation, finishing fermentation when the number of faecal coliform groups in the fermented material is less than or equal to 20, and taking the fermented product as the bio-organic fertilizer.

To help better understand the technical scheme of the present invention, the following examples are provided for illustrating the bio-organic fertilizer and the preparation process thereof according to the present invention.

Example one

The raw materials of the bio-organic fertilizer comprise, by weight, 60 parts of animal wastes, 27 parts of oil bran, 2 parts of molasses, 8 parts of calcium dihydrogen phosphate and 0.1 part of composite probiotic liquid. The composite probiotic liquid is formed by mixing bacillus subtilis liquid, lactobacillus plantarum liquid and saccharomyces cerevisiae liquid, wherein the three kinds of liquid are respectively 20 parts by weight of bacillus subtilis liquid, 40 parts by weight of lactobacillus plantarum liquid and 27 parts by weight of saccharomyces cerevisiae liquid. The bacterial contents of the three bacterial liquids are respectively as follows: the bacterial content of the bacillus subtilis liquid is more than or equal to 1.0 multiplied by 10¹⁰cfu/mL, the bacterial content of the lactobacillus plantarum bacterial liquid is more than or equal to 4.0 multiplied by 10¹¹cfu/mL, the bacterium content of the saccharomyces cerevisiae liquid is more than or equal to 5.0 multiplied by 10⁸cfu/mL。

The biological organic fertilizer is prepared by the following steps.

Step 1, preparing composite probiotic liquid, and uniformly mixing the three types of bacteria liquid to obtain the composite probiotic liquid.

And 2, mixing the raw materials to prepare a mixed material, adding the animal waste, the oil bran, the molasses, the monocalcium phosphate and the composite probiotic liquid together, stirring and mixing, and adding clear water while stirring to adjust the water content of the mixed material to 20%.

And 3, fermenting, wherein the initial pH of the materials is natural, the initial pH of the mixed materials is natural, the mixture is subjected to facultative fermentation for 33 days at the temperature higher than 4 ℃, the fermentation is finished when the number of faecal coliform groups in the fermented materials is less than or equal to 20, and the fermented product is used as the bio-organic fertilizer 1.

Example two

The raw materials of the bio-organic fertilizer comprise, by weight, 64 parts of animal wastes, 25 parts of oil bran, 4 parts of molasses, 10 parts of calcium dihydrogen phosphate and 0.13 part of composite probiotic liquid. The composite probiotic liquid is formed by mixing bacillus subtilis liquid, lactobacillus plantarum liquid and saccharomyces cerevisiae liquid, wherein the three kinds of liquid are 25 parts by weight of bacillus subtilis liquid, 37 parts by weight of lactobacillus plantarum liquid and 30 parts by weight of saccharomyces cerevisiae liquid. The bacterial contents of the three bacterial liquids are respectively as follows: the bacterial content of the bacillus subtilis liquid is more than or equal to 1.0 multiplied by 10¹⁰cfu/mL, the bacterial content of the lactobacillus plantarum bacterial liquid is more than or equal to 4.0 multiplied by 10¹¹cfu/mL, the bacterium content of the saccharomyces cerevisiae liquid is more than or equal to 5.0 multiplied by 10⁸cfu/mL。

The biological organic fertilizer is prepared by the following steps.

Step 1, preparing composite probiotic liquid, and uniformly mixing the three types of bacteria liquid to obtain the composite probiotic liquid.

And 2, mixing the raw materials to prepare a mixed material, adding the animal waste, the oil bran, the molasses, the monocalcium phosphate and the composite probiotic liquid together, stirring and mixing, and adding clear water while stirring to adjust the water content of the mixed material to 50%.

And 3, fermenting, namely fermenting naturally the initial pH of the materials, fermenting naturally the initial pH of the mixed materials for 35 days at the temperature higher than 4 ℃, finishing fermentation when the number of faecal coliform groups in the fermented materials is less than or equal to 20, and taking the fermented product as a bio-organic fertilizer 2.

EXAMPLE III

The raw materials of the bio-organic fertilizer comprise 67 parts of animal wastes, 23 parts of oil bran, 3 parts of molasses, 7 parts of calcium dihydrogen phosphate and 0.17 part of composite probiotic liquid by weight. The composite probiotic liquid is formed by mixing bacillus subtilis liquid, lactobacillus plantarum liquid and saccharomyces cerevisiae liquid, and the three kinds of liquid are 30 parts by weight of the bacillus subtilis liquid, 33 parts by weight of the lactobacillus plantarum liquid and 25 parts by weight of the saccharomyces cerevisiae liquid. The bacterial contents of the three bacterial liquids are respectively as follows: bacterium-containing bacillus subtilis liquidThe amount is more than or equal to 1.0 multiplied by 10¹⁰cfu/mL, the bacterial content of the lactobacillus plantarum bacterial liquid is more than or equal to 4.0 multiplied by 10¹¹cfu/mL, the bacterium content of the saccharomyces cerevisiae liquid is more than or equal to 5.0 multiplied by 10⁸cfu/mL。

The biological organic fertilizer is prepared by the following steps.

Step 1, preparing composite probiotic liquid, and uniformly mixing the three types of bacteria liquid to obtain the composite probiotic liquid.

And 2, mixing the raw materials to prepare a mixed material, adding the animal waste, the oil bran, the molasses, the monocalcium phosphate and the composite probiotic liquid together, stirring and mixing, and adding clear water while stirring to adjust the water content of the mixed material to 60%.

And 3, fermenting, wherein the initial pH of the materials is natural, the initial pH of the mixed materials is natural, the mixture is subjected to facultative fermentation for 30 days at the temperature higher than 4 ℃, the fermentation is finished when the number of faecal coliform groups in the fermented materials is less than or equal to 20, and the fermented product is used as a bio-organic fertilizer 3.

Example four

The raw materials of the bio-organic fertilizer comprise, by weight, 70 parts of animal wastes, 21 parts of oil bran, 5 parts of molasses, 9 parts of calcium dihydrogen phosphate and 0.2 part of composite probiotic liquid. The composite probiotic liquid is formed by mixing bacillus subtilis liquid, lactobacillus plantarum liquid and saccharomyces cerevisiae liquid, wherein the three kinds of liquid are 35 parts by weight of bacillus subtilis liquid, 30 parts by weight of lactobacillus plantarum liquid and 20 parts by weight of saccharomyces cerevisiae liquid. The bacterial contents of the three bacterial liquids are respectively as follows: the bacterial content of the bacillus subtilis liquid is more than or equal to 1.0 multiplied by 10¹⁰cfu/mL, the bacterial content of the lactobacillus plantarum bacterial liquid is more than or equal to 4.0 multiplied by 10¹¹cfu/mL, the bacterium content of the saccharomyces cerevisiae liquid is more than or equal to 5.0 multiplied by 10⁸cfu/mL。

The biological organic fertilizer is prepared by the following steps.

Step 1, preparing composite probiotic liquid, and uniformly mixing the three types of bacteria liquid to obtain the composite probiotic liquid.

And 2, mixing the raw materials to prepare a mixed material, adding the animal waste, the oil bran, the molasses, the monocalcium phosphate and the composite probiotic liquid together, stirring and mixing, and adding clear water while stirring to adjust the water content of the mixed material to 85%.

And 3, fermenting, wherein the initial pH of the materials is natural, the initial pH of the mixed materials is natural, the mixture is subjected to facultative fermentation for 40 days at the temperature higher than 4 ℃, the fermentation is finished when the number of faecal coliform groups in the fermented materials is less than or equal to 20, and the fermented product is used as a bio-organic fertilizer 4.

In order to help better understand the technical scheme of the invention, a test example of soybean planting is provided below for illustrating the application effect of the invention.

The first test example: effect of bioorganic fertilizer on Soybean growth

A soybean planting test of 120 days is carried out in the same district in Daqing city, the same field is selected, the basic physicochemical property of the field soil is pH value 7.48, the organic matter content of the soil of the plough layer is 26.5g/kg, the quick-acting phosphorus content is 28mg/kg, the quick-acting potassium content is 185mg/kg, and the alkaline hydrolysis nitrogen content is 126 mg/kg. The experimental design 5 groups comprise 4 experimental groups and 1 control group, each group of experimental designs comprises 3 experimental cells, and the area of each experimental cell is 20m²All test cells are randomly distributed.

The variety of the test planting is selected as Anxiao No. 4, and the double-row precision seeding on the ridge is adopted in the late 5 months, the seeding quantity is 5kg/667m². The control group does not apply the biological organic fertilizer, the test group applies the biological organic fertilizer prepared by the invention as base fertilizer, and the fertilizing amount is 1500kg/hm². The same general management is adopted for each cell, and the cells are harvested in the last 9 months.

After the soybeans are sowed for 15 days, 4 soybean seedlings with 1m double rows are taken from each cell to check the number of the seedlings, 10 soybean seedlings are dug in the cell 15 days after the soybeans are sowed, the root system fresh weight, the root number and the stem leaf fresh weight of each soybean are checked after the soybeans are cleaned, the average emergence rate, the average root system fresh weight, the average root number and the average stem leaf fresh weight of each group of soybeans are calculated, and the results are shown in table 1.

TABLE 1

As can be seen from the data in Table 1, the rate of emergence, the fresh weight of the root system, the number of roots and the fresh weight of stems and leaves of the 4 groups of soybeans applied with the bio-organic fertilizer are all obviously higher than those of the control group. Therefore, the prepared biological organic fertilizer 1-4 can obviously promote the germination, rooting and seedling growth of the soybeans.

Before harvesting, 10 plants are randomly selected from each cell for seed test, and the plant height and the hundred grain weight are counted. After harvesting, the cell yield was counted. The average plant height, average grain weight and average cell yield of each group of soybeans were calculated after the experiment was completed, and the results are shown in table 2.

TABLE 2

As can be seen from the data in Table 2, the 4 groups of soybeans applied with the bio-organic fertilizer are obviously higher than the control group in three characters of plant height, hundred grain weight and cell yield. Therefore, the prepared bio-organic fertilizer 1-4 can obviously promote the growth of soybeans and improve the yield of the soybeans.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent structural changes made by using the contents of the present specification, or any other related technical fields directly or indirectly, are included in the scope of the present invention.