Light irradiation device

文档序号：862408 发布日期：2021-03-16 浏览：7次中文

阅读说明：本技术 光照射装置 (Light irradiation device ) 是由裵熙镐尹永民李雅永于 2020-01-23 设计创作，主要内容包括：光照射装置包括：壳体；基板,设置于所述壳体内；及光源,贴装于所述基板上。所述光源包括：第一光源,提供为至少一个,并且射出蓝色波段的第一光；第二光源,提供为至少一个,并且射出紫外线波段的第二光；及控制部,控制所述第一光源及所述第二光源的发光与否,使所述第一光和所述第二光依次射出,或使所述第一光和所述第二光重叠而射出,或即便并不重叠也使所述第一光和所述第二光在接近的时期射出。所述第二光源的剂量可以小于所述第一光源的剂量的1/10。(The light irradiation device includes: a housing; a substrate disposed within the housing; and the light source is attached to the substrate. The light source includes: a first light source provided as at least one and emitting first light of a blue wavelength band; a second light source provided as at least one and emitting second light of an ultraviolet band; and a control unit that controls whether or not the first light source and the second light source emit light, and that sequentially emits the first light and the second light, or that emits the first light and the second light while overlapping each other, or that emits the first light and the second light at a timing when they approach each other even if they do not overlap each other. The dose of the second light source may be less than 1/10 of the dose of the first light source.)

1. A light irradiation device comprising:

a housing;

a substrate disposed within the housing; and

a light source attached to the substrate,

wherein the light source includes:

a first light source provided as at least one and emitting first light of a blue wavelength band;

a second light source provided as at least one and emitting second light of an ultraviolet band; and

a control unit that controls whether or not the first light source and the second light source emit light, sequentially emits the first light and the second light, or emits the first light and the second light while overlapping each other, or emits the first light and the second light at a timing when they approach each other even if they do not overlap each other,

wherein the dose of the second light source is less than 1/10 of the dose of the first light source.

2. The light irradiation apparatus according to claim 1,

the control unit controls the first light source and the second light source to emit the second light after the first light starts to be emitted.

3. The light irradiation apparatus according to claim 1,

the second light corresponds to at least one of UVA, UVB and UVC bands.

4. The light irradiation apparatus according to claim 2,

the first light has a wavelength band of about 400nm to about 500 nm.

5. The light irradiation apparatus according to claim 1,

the first light further includes light of a wavelength band corresponding to visible light.

6. The light irradiation apparatus according to claim 5,

the first light has a wavelength band of about 380nm to about 780 nm.

7. The light irradiation apparatus according to claim 1,

the second light has a wavelength band of about 240nm to about 280 nm.

8. The light irradiation apparatus according to claim 7,

the first light is illuminated during a first time and the second light is illuminated during a second time that is shorter than the first time.

9. The light irradiation apparatus according to claim 8,

the second light starts irradiation after the irradiation of the first light is ended.

10. The light irradiation apparatus according to claim 8,

the second light starts irradiation before the irradiation of the first light is ended, and at least a part of the first time and the second time have an interval overlapping each other.

11. The light irradiation apparatus according to claim 8,

the first light is continuously irradiated.

12. The light irradiation apparatus according to claim 8,

the second light is discontinuously irradiated.

13. The light irradiation apparatus according to claim 8,

the second light is periodically illuminated.

14. The light irradiation apparatus according to claim 1,

the light irradiation device is used for human body treatment.

15. The light irradiation apparatus according to claim 14,

the light irradiation device is used for acute wound treatment.

16. The light irradiation apparatus according to claim 1,

the second light source emits the second light within an allowable dose if a dose in a range that is not harmful every day when the second light is applied to a human body is set as an allowable dose.

17. The light irradiation apparatus according to claim 16,

the second light is at about 30J/m²To about 1,000,000J/m²The dose of irradiation of (2).

18. A therapeutic-light irradiation device comprising:

a first light source provided as at least one and emitting first light of a blue wavelength band;

a second light source provided as at least one and emitting second light of an ultraviolet band; and

and a control unit that controls the first light source and the second light source to sequentially emit the second light source after emitting the first light source.

19. The therapeutic-light irradiation device according to claim 18,

the first light has a wavelength band of about 400nm to about 500nm, and the second light has a wavelength band of about 240nm to about 280 nm.

20. The therapeutic-light irradiation device according to claim 19,

the first light is illuminated during a first time and the second light is illuminated during a second time that is shorter than the first time.

Technical Field

The present invention relates to a light irradiation device, and more particularly, to a light irradiation device used for treatment.

Background

Recently, various therapeutic apparatuses using ultraviolet rays have been developed. Conventionally, ultraviolet rays are known to have a sterilizing effect, and existing ultraviolet ray treatment apparatuses are used in the following manner: a conventional uv lamp is used and activated near the skin to apply uv light to the area to be treated.

However, ultraviolet rays not only have a bactericidal effect, but also have side effects of inducing skin aging, cancer, and the like. Accordingly, there is a need for a method capable of obtaining a sterilization effect in a safe manner without affecting the human body.

Disclosure of Invention

Technical subject

The invention aims to provide a light irradiation device which can improve the sterilization effect while minimizing the side effect on the human body.

Technical scheme

A light irradiation apparatus according to an embodiment of the present invention includes: a housing; a substrate disposed within the housing; and a light source attached to the substrate, wherein the light source includes: a first light source provided as at least one and emitting first light of a blue wavelength band; a second light source provided as at least one and emitting second light of an ultraviolet band; and a control unit that controls whether or not the first light source and the second light source emit light, and sequentially emits the first light and the second light, or emits the first light and the second light while the first light and the second light are overlapped, or emits the first light and the second light at a timing when the first light and the second light approach each other even if the first light and the second light are not overlapped, wherein a dose of the second light source is 1/10 smaller than a dose of the first light source.

In an embodiment of the present invention, the control unit may control the first light source and the second light source to emit the second light after the first light starts to be emitted.

In an embodiment of the invention, the second light may correspond to at least one of UVA, UVB and UVC bands.

In an embodiment of the present invention, the first light may have a wavelength band of about 400nm to about 500 nm. The first light may further include light of a wavelength band corresponding to visible light, and the first light may have a wavelength band of about 380nm to about 780 nm. In this case, the spectrum of the first light may have an area of about 55% or more of the area of the normalized solar light spectrum, and the peak of the first light may have a deviation (deviation) of about 0.14 or less of the normalized solar light spectrum.

In an embodiment of the invention, the second light may have a wavelength band of about 240nm to about 280 nm.

In an embodiment of the invention, the first light is illuminated during a first time and the second light is illuminated during a second time shorter than the first time. In an embodiment of the present invention, the second light may start to be irradiated after irradiation of the first light is ended, the second light may start to be irradiated before irradiation of the first light is ended, and at least a part of the first time and the second time may have intervals overlapping each other. Also, the first light may be continuously irradiated, and the second light may be discontinuously irradiated. In an embodiment of the invention, the second light may be periodically irradiated.

In an embodiment of the invention, the light irradiation device may be used for human body treatment, for example, acute wound treatment.

In an embodiment of the present invention, if a dose in a range that is not harmful every day when the second light is applied to the human body is set as an allowable dose, the second light source may emit the second light within the allowable dose. In one embodiment of the present invention, the second light is emitted at about 30J/m²To about 1,000,000J/m²The dose of irradiation of (2).

Technical effects

According to an embodiment of the present invention, there is provided a light irradiation device that minimizes side effects on a human body and has a high sterilization effect

Drawings

Fig. 1 is a plan view illustrating a light irradiation device according to an embodiment of the present invention.

Fig. 2 is a block diagram illustrating a light irradiation apparatus according to an embodiment of the present invention.

Fig. 3a and 3c are diagrams illustrating a driving method of a light irradiation device according to an embodiment of the present invention, and are diagrams illustrating times corresponding to on/off of a first light source and a second light source.

Fig. 4a and 4b are diagrams illustrating a driving method of a light irradiation device according to an embodiment of the present invention in a case where first light and second light are sequentially irradiated, and are diagrams illustrating times corresponding to on/off of a first light source and a second light source.

Fig. 5a to 5c are diagrams illustrating a driving method of a light irradiation device according to an embodiment of the present invention, and are diagrams illustrating times corresponding to on/off of a first light source and a second light source.

Fig. 6 is a spectrum of light emitted from a first light source in a light emitting device according to an embodiment of the present invention.

Fig. 7a is a plan view of a light irradiation device according to an embodiment of the present invention, and fig. 7b is a sectional view taken along line I-I' of fig. 7 a.

Fig. 8 and 9 are diagrams illustrating an example in which a lighting device according to an embodiment of the present invention is implemented as a product.

Fig. 11a is a graph showing the result of testing the sterilizing power of the first light, and fig. 11b is a graph showing the result of testing the sterilizing power of the second light.

Fig. 12a is a graph showing the number of bacteria in the case of irradiating the first light alone, the case of irradiating the second light alone, and the case of irradiating the first light and the second light in combination, and fig. 12b is a graph showing the bactericidal activity in the case of irradiating the first light alone, the case of irradiating the second light alone, and the case of irradiating the first light and the second light in combination.

Fig. 13a is a graph showing the number of bacteria in the case of irradiation with the combination order of the first light and the second light set differently, and fig. 13b is a graph showing the bacterial force in the case of irradiation with the combination order of the first light and the second light set differently.

Fig. 14a is a graph showing the number of bacteria when the first light and the second light are sequentially irradiated and the light quantity of the first light is changed in the ex vivo condition, and fig. 14b is a graph showing the bactericidal power when the first light and the second light are sequentially irradiated and the light quantity of the first light is changed in the ex vivo condition.

Fig. 15a is a graph showing the number of bacteria when the first light and the second light are sequentially irradiated and the light quantity of the first light is changed under in-vivo conditions, and fig. 15b is a graph showing the bactericidal power when the first light and the second light are sequentially irradiated and the light quantity of the first light is changed under in-vivo conditions.

Fig. 16 is a graph showing changes in bactericidal power with date under in vivo conditions.

Fig. 17 is a graph showing the results of measuring the number of bacteria against date under in vivo conditions.

Fig. 18 is a graph showing the change in wound area with date under in vivo conditions.

Fig. 19a and 19b are photographs of the shape of the area of the wound as the date and time passes, fig. 19a is a photograph of the wound without the irradiation group, and fig. 19b is a photograph of the wound with the irradiation group.

FIG. 20a is a graph showing the content of thymine dimer in the tissue in percentage, and FIG. 20b is a graph showing the degree of tissue luminescence stained by DCFH-DA.

Best mode for carrying out the invention

The present invention is capable of various modifications and of various forms, and specific embodiments thereof are shown by way of example in the drawings and will herein be described in detail. However, the present invention is not limited to the specific forms disclosed, and all modifications, equivalents, and alternatives included in the spirit and technical scope of the present invention are to be understood.

Hereinafter, preferred embodiments of the present invention will be described in more detail with reference to the accompanying drawings.

The present invention relates to a light irradiation device that applies sterilization light to an object to be sterilized to perform sterilization. In particular, the light irradiation device according to an embodiment of the present invention can be used for the purpose of healing wounds where healing of wounds is required. In the case where the object to be sterilized is a human body and a wound is formed on the skin, it is necessary to sterilize pathogens at the wound site, and the sterilization apparatus according to an embodiment of the present invention may be used to sterilize pathogens in the wound. The pathogen (pathogen) refers to microorganisms such as bacteria, viruses, bacteria, fungi, protists, and molds. The light irradiation device according to an embodiment of the present invention can be used for various wounds such as wounds, ulcers (ulcers), infection of incised parts (surgical site infection), lacerations (lacerations), incised wounds (incised wounds), and incised wounds (incised wounds).

Fig. 1 is a plan view illustrating a light irradiation device according to an embodiment of the present invention.

The light irradiation apparatus 100 according to an embodiment of the present invention includes: a first light source 30 that emits first light; a second light source 40 emitting second light; and a substrate 20 on which the first light source 30 and the second light source 40 are mounted.

The first and second light sources 30 and 40 are attached to the substrate 20, and the substrate 20 is not particularly limited as long as the first and second light sources 30 and 40 can be attached thereto, and may be provided in various forms. The substrate 20 may be provided in a form including a wire so as to be able to supply power to the first light source 30 and the second light source 40. The substrate 20 may be formed of, for example, a metal substrate on which wiring is formed, a printed circuit board, or the like.

The first light source 30 emits first light of a blue wavelength band among visible light wavelength bands. The first light may correspond to light of a wavelength band of about 400nm to about 500 nm. In one embodiment of the present invention, the first light may be light of a wavelength band of about 400nm to about 420 nm. In an embodiment of the present invention, in more detail, the first light may be light having a wavelength of 405 nm.

The first light acts on a photosensitizer (photosensitizer) present in microorganisms such as bacteria, molds, and the like to cause damage to cells and induce death of the microorganisms. The first light corresponds to an absorption wavelength of porphyrin (porphyrin) as a photosensitizer present in bacteria. In particular, the first light exhibits a higher bactericidal activity at wavelengths of 400nm to 420nm, 455nm to 470nm, which corresponds to an absorption band of porphyrin (porphyrin) as a photosensitizer. Porphyrins are pigments (pigments) that are essential in the oxygen transport process in cells. In particular, porphyrins exhibit high absorbance at wavelengths from about 402nm to about 420nm, and also absorb wavelengths from about 455nm to 470 nm. In an embodiment of the present invention, since the content of porphyrin varies according to the type of bacteria, the wavelength and intensity of the first light can be adjusted to be used for killing specific bacteria. When the first light is applied to the bacterium, the porphyrin in the bacterium absorbs the first light, and active oxygen (reactive oxygen species) is generated in the cell of the bacterium by the energy of the first light. The active oxygen accumulates in the cell of the bacterium to oxidize the cell wall of the bacterium, and as a result, has a bactericidal effect.

The second light source 40 emits second light in the ultraviolet band. That is, the second light may be light of a wavelength band of about 100nm to about 400nm, and may be UVA, UVB, UVC. UVA may have a wavelength band of about 315nm to about 400nm, UVB may have a wavelength band of about 280nm to about 315nm, and UVC may have a wavelength band of about 100nm to about 280 nm. In an embodiment of the present invention, the second light may correspond to UVC, and in this case, may have a wavelength band of about 240nm to about 280 nm. In an embodiment of the present invention, in more detail, the second light may be light having a wavelength of 275 nm.

If the second light is applied to the bacteria, the DNA in the bacteria absorbs the second light, and the change in the structure of the DNA is generated by the energy of the second light. The reason why DNA is broken in the binding between thymine and adenine in DNA by the absorption of light is that purine, pyrimidine or the like which is a base constituting DNA strongly absorbs ultraviolet light and absorbs light to form thymine dimer. Through such a process, deformation of DNA occurs, and the deformed DNA causes death of the bacterium due to lack of cell proliferation. The DNA may absorb light in a wavelength band of about 240nm to about 280 nm.

Fig. 2 is a block diagram illustrating a light irradiation apparatus according to an embodiment of the present invention.

Referring to fig. 2, a light irradiation apparatus according to an embodiment of the present invention may include: a first light source 30 that emits first light; a second light source 40 emitting second light; a control unit 50 for controlling the emission of the first light source 30 and the second light source 40; the power supply unit 60 supplies power to the control unit 50, the first light source 30, and the second light source 40.

As described above, the first light source 30 and the second light source 40 can emit the first light including the blue wavelength band and the second light including the ultraviolet wavelength band, respectively. In an embodiment of the present invention, the first light source 30 and the second light source 40 can be implemented by using various light sources. For example, a plurality of light sources such as a light emitting diode, a halogen lamp, a fluorescent lamp, a gas discharge lamp, and a laser may be used as each of the first light source 30 and the second light source 40, and the types thereof are not limited.

The control unit 50 can control whether or not light is emitted from the first light source 30 and the second light source 40, the amount of light, the intensity of light, the emission time, and the like. The control section 50 can control whether or not light is emitted, the amount of light, the intensity of light, and the emission time in various ways.

The power supply unit 60 is electrically connected to the first light source 30, the second light source 40 and the control unit 50 to supply power to the first light source 30, the second light source 40 and the control unit 50. Although the power supply unit 60 supplies power to the first light source 30 and the second light source 40 through the control unit 50 in the drawings, the present invention is not limited thereto, and the power supply unit 60 may be directly connected to the first light source 30 and the second light source 40 to supply power to the first light source 30 and the second light source 40.

The light irradiation device 100 may further include an optical unit that selectively collects or disperses the light emitted from the first light source 30 and the second light source 40. The optical unit may collect light generated by the first and second light sources 30 and 40 to a narrow range or a wide range as necessary. Alternatively, the light may be concentrated or dispersed in a uniform form or a non-uniform form depending on the position to be irradiated. The optical portion may include at least one lens as necessary, and the lens may perform various functions such as light collection, dispersion, homogenization, non-homogenization, and the like from the first light source 30 and the second light source 40.

For example, in the case of irradiating light to a narrow area using the light emitting device 100 according to an embodiment of the present invention, a lens for condensing light of the first and second light sources 30 and 40 may be used, and conversely, in the case of supplying light to a wide area (e.g., the entire room) using the light emitting device 100 according to an embodiment of the present invention, a lens for dispersing light may be used.

In the present embodiment, the control part 50 drives the first light source 30 and the second light source 40 simultaneously or individually, respectively. That is, the first and second light sources 30 and 40 may be simultaneously turned on/off, or the first and second light sources 30 and 40 may be individually turned on/off. Also, the intensities of the emitted light from the first light source 30 and the second light source 40 (i.e., the first light and the second light) may also be controlled simultaneously or individually.

In one embodiment of the present invention, the control unit 50 may control the amount of the ultraviolet rays to be irradiated at 3mJ/cm²The following. Particularly, in the case of UVC, the control part 50 maintains the daily dose at 3mJ/cm²The following. In addition, in the case of UVA, the ultraviolet irradiation amount may be maintained not to exceed 1J/cm in the case that the daily irradiation time is less than 1000 seconds²When the daily irradiation time is 1000 seconds or more, the ultraviolet irradiation amount may be maintained at 1mW/cm or less²。

In an embodiment of the present invention, the distances from the first light source 30 and the second light source 40 to the sterilization target may be set to be various. For example, the intensity of the light from the first light source 30 and the second light source 40, the type of object to be sterilized, the area or volume to be sterilized, the target substance to be sterilized (e.g., bacteria, etc.), and the like may be variously changed. In a similar manner, in an embodiment of the invention, the light irradiation time of the first light source 30 and the second light source 40 can also be set variously.

In the light irradiation device according to the embodiment of the present invention, if the first light emitted from the first light source is denoted as L1, the second light emitted from the second light source is denoted as L2, and the elapse of time is denoted as T, the first light source is turned on for the first time T1 to irradiate the first light L1, and the second light source is turned on for the second time T2 to irradiate the second light L2. In the present embodiment, the first time t1 of irradiating the first light L1 may be longer than the second time t2 of irradiating the second light L2. In the case of the second light L2, the influence on the human body is large in particular, and therefore the second light L2 can be irradiated during a time shorter than the time in which the first light L1 is irradiated. For example, the first light source may apply light for a time of about 10 minutes or so, and the second light source may apply light for a time of about 10 seconds or less.

Although the irradiation times t1, t2 and the light amounts at the time of irradiation of the first light L2 and the second light L2 emitted from the first light source and the second light source can be variously changed, the total dose to the subject to be sterilized is set within a range harmless to the human body. In particular, when the second light L2 is applied to the human body, if a dose in a harmless range per day is referred to as an allowable dose, the second light source may emit the second light L2 within the allowable dose. The dose may differ depending on the harmfulness of the light emitted from the first and second light sources, however in one embodiment of the invention, the dose of the second light source may be relative to the dose emitted from the first light sourceThe dosage of the first light source is within 1/10, and in another embodiment, the dosage of the second light source may also be 1/20 relative to the dosage of the first light source. For example, the allowable dose of the second light L2 may be about 30J/m²To about 1,000,000J/m²。

As shown in fig. 3a and 3c, the first light L1 and the second light L2 may start to be irradiated at the same time, or may start to be irradiated at different times from each other. In the case where the first light L1 and the second light L2 start to be irradiated at different times from each other, the first light L1 may be irradiated first or the second light L2 may be irradiated first. The irradiation times of the first light L1 and the second light L2 may overlap each other or may not overlap each other. In the case where the times of irradiation of the first light L1 and the second light L2 do not overlap, the interval between the times of application of the first light L1 and the second light L2 may be set to be short. For example, the interval between the times of applying the first light L1 and the second light L2 may be within hours, or within minutes, or within seconds.

The sterilization apparatus according to an embodiment of the present invention exhibits a significantly higher sterilization effect than that of the first light alone or the second light alone, by virtue of the synergistic effect that can be obtained by applying the first light and the second light simultaneously or applying the first light and the second light in adjacent times, if not simultaneously.

The sterilization apparatus according to an embodiment of the present invention employs the sterilization principle of both the first light and the second light, wherein the first light induces the generation of the reactive oxygen species by the photosensitizer, and the second light generates the thymine dimer to induce the DNA damage. Embodiments of the present invention mix the use of the first light source and the second light source to obtain a significantly higher sterilization effect in a relatively short time using less energy than the case where each light source is used alone.

The bacteria to which chemical and physical stresses are applied have a rapidly increased killing rate even by additionally applying other types of weak stimuli, and in the embodiment of the present invention, different stresses are induced to the bacteria by two different sterilization mechanisms of the first light and the second light corresponding to the blue light and the ultraviolet light. Accordingly, the synergistic effect of this stress can be utilized to kill bacteria with less energy than when using two light sources alone. According to an embodiment of the present invention, the second light is irradiated under an energy condition that is harmless to the biological tissue of the sterilization object and the first light is mixed to obtain the sterilization synergistic effect by both the light sources, whereby the present invention can obtain an effective sterilization effect in a short time without damaging the human tissue even when the sterilization object is a human body.

In contrast, in the case of using only the first light, although it is harmless to the human body, the sterilizing power is relatively weak, and thus it is necessary to irradiate at high energy for a long time, and in the case of using only the second light, although it is excellent, it is necessary to pay attention to the problem that it is harmful to the human body.

As described above, it may be used in the case of sterilizing a plurality of pathogens according to an embodiment of the present invention. In particular, the light irradiation device according to an embodiment of the present invention can be used in a case where an acute infected wound is irradiated with sterilizing light to sterilize infectious bacteria at an initial stage, and as a result, an effect of shortening a wound healing period can be obtained. In the case of acute wounds, it is of utmost importance to reduce the number of infectious bacteria in the early stages of the wound healing process. If the initial sterilization is not sufficiently performed in the acute wound, the wound may not be normally healed, and a chronic wound in which the wound is not healed even for three months or more may develop.

However, microorganisms such as bacteria, and mold present in animals and various articles can be sterilized in addition to human bodies, and the object to be treated by the sterilization apparatus according to the embodiment of the present invention is not limited to human bodies, and can be extended to animals and various articles.

According to an embodiment of the present invention, as described above, in the case where the first light and the second light emitted from the first light source and the second light source are irradiated at the same time or are irradiated in close time even if not irradiated at the same time, the sterilization effect is remarkably increased. In addition to this, according to an embodiment of the present invention, the case of sequentially irradiating the first light and the second light may realize higher sterilization efficiency than the case of sequentially irradiating the second light and the first light. Accordingly, according to an embodiment of the present invention, sterilization efficiency can be maximized through a process of sequentially applying the first light and the second light to the object to be sterilized.

According to an embodiment of the present invention, before the second light is irradiated, the first light may be applied to the object to be sterilized for a predetermined time period, and then the second light is irradiated. Accordingly, DNA can be prevented from recovering from damage after the pre-irradiation with the first light, and as a result, an excellent and high bactericidal effect can be obtained with a smaller dose than in the case of irradiating the first light alone. In addition, in the case of the second light, although the sterilizing power for the object to be sterilized is excellent, when the object is exposed to the human body for a long time, there is a bad influence on the human body, for example, there is an influence of skin aging or induction of cancer. Therefore, there is a very limited problem in the operation of separately applying the second light to the object to be sterilized. However, according to an embodiment of the present invention, the second light is irradiated on the basis of the irradiation of the first light, so that a significant sterilization effect can be obtained even with a small amount of irradiation, as compared with the case of single irradiation.

In an embodiment of the present invention, when the second light needs to be sequentially emitted in addition to the first light, the light amount of the second light needs to be controlled. In an embodiment of the present invention, while a sterilization synergistic effect by sequentially irradiating the first light and the second light can be obtained, an influence on a human body can be minimized. For this reason, when the first light source and the second light source are turned on/off, a mode of continuously emitting light, a mode of sequentially decreasing or increasing the intensity of light, a blinking mode, a hybrid mode, or the like may be employed.

Referring to fig. 4a and 4b, in an embodiment of the present invention, the irradiation of the first light L1 may be performed first, and then the irradiation of the second light L2 may be performed. In the case where the second light L2 is irradiated after the first light L1 is irradiated first, the sterilizing power is significantly increased as compared with the case where the first light L1 is irradiated after the second light L2 is irradiated first. In the case where the second light L2 is irradiated first and then the first light L1 is irradiated, the bacteria growth inhibiting effect by the second light L2 may be reduced by the irradiation of the first light L1 because the following effects are exhibited: even if a part of the structure of the DNA is deformed by the second light L2, the deformed DNA is photo-revived (photo-revitalization) by irradiation of the first light L1 including a visible ray band. Since the bacteria recovered by the irradiation of the first light L1 are recovered to a state capable of growing again, the final sterilizing power may be lower than that in the case of sequentially irradiating the first light L1 and the second light L2, even though the sterilizing power is good as a whole.

In contrast, in the case where the first light L1 is applied to the object to be sterilized by the light irradiation apparatus according to the embodiment of the present invention and then the second light L2 is sequentially applied, active oxygen in bacteria is generated by the first light L1 that is irradiated first, and thus oxidative stress occurs in the bacteria. In this state, additional sterilization is caused by the second light L2 that is irradiated later, and the degree of sterilization of bacteria is significantly increased even with a small irradiation amount.

In the present embodiment, the time point of applying the second light L2 may be different within the limits of sequentially applying the first light L1 and the second light L2. For example, as shown in fig. 3a, the irradiation of the second light L2 may be started after the irradiation of the first light L1 is completed, and as shown in fig. 3b, although the irradiation of the first light L1 is not ended, the irradiation of the second light L2 may be started. In this case, the time points at which the first light L1 and the second light L2 are applied may partially overlap each other, so that at least a portion of the first time and the second time may have intervals that overlap each other.

The light irradiation device according to the embodiment of the present invention may be driven by the control part in various forms within the limits of sequentially irradiating the first light L1 and the second light L2.

Fig. 5a to 5c are diagrams illustrating a driving method of a light irradiation device according to an embodiment of the present invention, which illustrate times corresponding to on/off of a first light source and a second light source.

Referring to fig. 5a, first light L1 and second light L2 may be periodically irradiated to an object to be sterilized. That is, after the first light L1 is irradiated to the sterilization object during the first time t1 and the second light L2 is irradiated to the sterilization opposite direction during the second time t2, the irradiation of the first light L1 and the second light L2 may be repeated. Such repetition period and repetition number may be different depending on the kind, total amount, and the like of the object to be sterilized. Here, the repetition period and the number of times of the first light L1 and the second light L2 may be determined such that the total dose of the first light L1 and the total dose of the second light L2 are values below the allowable dose allowed by the human body.

Referring to fig. 5b, when the first light L1 and the second light L2 are applied, the first light L1 may be continuously applied to the sterilization object without interruption within the limit of applying the second light L2 after the first light L1 is applied. In contrast, the second light L2 is not continuously provided but discontinuously provided in such a manner as to overlap with the first light L1.

As shown, the first light L1 may be continuously applied to the sterilization object during the first time t1 without interruption, and after the application of the first light L1 is performed to some extent, the second light L2 may be applied to the sterilization object during the second time t2 in the middle of the continuous application of the first light L1. The second light L2 may be repeatedly applied to the sterilization object periodically.

Referring to fig. 5c, when the first light L1 and the second light L2 are applied, the first light L1 may be continuously applied to the sterilization object without interruption within the limit of applying the second light L2 after the first light L1 is applied, or may be interrupted before the second light L2 is applied. As shown in the drawing, in the case where the first light L1 is applied to the sterilization object during the first time t1 time, the second light L2 may be applied for the second time t2 on the way of the application of the first light L1. Subsequently, after the application of the first light L1 is ended, the second light L2 may be applied during a third time t 3. Among them, the application time of the second light L2 may be applied to the sterilization object during different times from each other within a value below an allowable dose allowed to be safe for a human body. That is, the second time t2 and the third time t3 at which the second light L2 is applied may have different values from each other.

In an embodiment of the present invention, when the second light L2 is applied immediately after the interruption in the application of the first light L1, the sterilization effect may be the best, and the second light L2 may be sequentially applied without interruption in the state where the first light L1 is applied. However, the second light L2 is not applied immediately after the application of the first light L1 is interrupted, but the second light L2 may be applied after a part of time has elapsed, however, the interval may be very short. In contrast, in the case where the first light L1 and the second light L2 are sequentially applied to obtain a predetermined sterilizing effect, the subsequent sequential application of the first light and L1 and the second light L2 may be re-performed after a sufficient time has elapsed.

In an embodiment of the present invention, the first light source includes a blue wavelength capable of sterilization in a visible light band, but is not limited thereto, and may include light in other visible light regions including light in a blue band.

Fig. 6 is a spectrum of light emitted from a first light source in a light emitting device according to an embodiment of the present invention.

Referring to fig. 6, the first light source emits light in a wavelength band of about 380nm to about 750nm, which mostly corresponds to a visible light wavelength band. That is, the first light source corresponds to a light source that emits white light. In the present embodiment, the first light source includes light of a blue wavelength band that produces a synergistic effect in combination with the second light, so that the above-described sterilization effect can be obtained in the same manner.

In addition, the first light source in the present embodiment has a spectrum similar to that of sunlight as a form in which light of the entire wavelength band is uniformly mixed. However, the difference from the solar light is that the first light source according to an embodiment of the present invention emits light except for most of the ultraviolet band. The light source according to an embodiment of the present invention emits light having a wavelength band of about 380nm to about 780nm corresponding to substantially the entire wavelength band of visible light.

In one embodiment of the invention, the expression "similar to sunlight" means the following: when the normalized solar spectrum is used as a reference, the overlapping area is equal to or larger than a predetermined value, and the deviation of the peak value from the solar spectrum (the degree of exceeding when the peak value of the solar spectrum is used as a reference) is equal to or smaller than a predetermined value, as compared with the conventional invention. For example, in one embodiment of the present invention, the first light source may emit light having an area of about 55% or more with respect to the area of the normalized solar spectrum, and the peak of the first light may have a deviation (deviation) of about 0.14 or less with respect to the normalized solar spectrum.

In this manner, the first light has a spectrum similar to that of sunlight, and thus may have the same effect as in the case of frequent exposure to sunlight, whereby the synthesis of vitamin D becomes easy, or the prevalence rate of diseases such as myopia may be reduced.

Detailed Description

The light irradiation device according to an embodiment of the present invention may be implemented in various forms. Fig. 7a is a plan view of a light irradiation device according to an embodiment of the present invention, and fig. 7b is a sectional view taken along line I-I' of fig. 7 a.

Referring to fig. 7a and 7b, the light irradiation apparatus according to an embodiment of the present invention may include a first light source 30, a second light source 40, and a substrate 20 to which the first light source 30 and the second light source 40 are attached.

In the present embodiment, the first light source 30 may be provided in plurality, and the second light source 40 may also be provided in plurality. For example, the first and second light sources 30 and 40 may be provided in the same number, and may be arranged in alternating rows and columns with respect to each other as shown. However, the number of the first and second light sources 30 and 40 is not limited thereto, and the number of the first light sources 30 may be more or less than the number of the second light sources 40. Also, according to an embodiment of the present invention, the first and second light sources 30 and 40 may be regularly or irregularly arranged according to the number thereof.

The light irradiation device according to an embodiment of the present invention may further include a housing accommodating the first light source 30, the second light source 40, and the substrate 20. The housing may be provided with a transmission window through which light emitted from the first and second light sources 30 and 40 is transmitted, and the light emitted from the first and second light sources 30 and 40 may be provided toward the human body side through the transmission window.

In one embodiment of the present invention, the control unit 50 may be provided on the substrate 20 in various forms, for example, in a form of being formed as a separate circuit wiring on the substrate 20, or being formed as a separate core and attached to the substrate 20.

As described above, the sterilization apparatus according to an embodiment of the present invention can be applied to various other apparatuses requiring sterilization, and in particular, can be applied to an apparatus using a light source. Further, the present invention may be used as a lighting device, not exclusively for a sterilization device. For example, the sterilization apparatus according to an embodiment of the present invention may further include an additional light source for illumination for providing light to the predetermined space, and in this case, the additional light source may emit light in a visible light band. The additional light source may emit light corresponding to the entire spectrum of the visible light region, or may emit light corresponding to a spectrum of a specific color.

Alternatively, in an embodiment of the present invention, the first light source may emit light of a visible light band including light of a blue band without a separate additional light source. For example, the first light source emits light in a wavelength band of about 380nm to about 750nm, which mostly corresponds to the visible light wavelength band. In this case, the first light source integrally provides light of a visible light band while including light of a blue band that produces a synergistic effect in combination with the second light, so that the same sterilization effect as the above-described embodiment can be obtained. In this way, in the case where an additional light source that emits light in the visible light band is provided, or in the case where the first light source emits light in the visible light band, the light may have a spectrum similar to that of sunlight. In the case where the above light has a spectrum similar to that of sunlight, the same effect as in the case of frequent exposure to sunlight can be obtained, whereby the synthesis of vitamin D becomes easy and the prevalence of diseases such as myopia can be reduced.

The light emitting device according to an embodiment of the present invention may be implemented in various forms. Fig. 7a is a plan view of a light emitting device according to an embodiment of the present invention, and fig. 7b is a sectional view taken along line I-I' of fig. 7 a.

Referring to fig. 7a and 7b, a light emitting device according to an embodiment of the present invention may include a first light source 30, a second light source 40, and a substrate 20 to which the first light source 30 and the second light source 40 are attached.

The light emitting device according to an embodiment of the present invention may further include a case accommodating the first light source 30, the second light source 40, and the substrate 20. The housing may be provided with a transmission window through which light emitted from the first and second light sources 30 and 40 is transmitted, and the light emitted from the first and second light sources 30 and 40 may be provided toward the human body side through the transmission window.

The light-emitting device can be realized in various forms and used for various purposes. For example, the light-emitting device according to an embodiment of the present invention can be applied to various places where illumination and sterilization are required, and can be used as an illumination device in particular. For example, it can be used in medical facilities such as operating rooms, hospitals, and the like, and lighting devices for public or personal hygiene. In particular, the illumination device according to an embodiment of the invention may be used for patient treatment purposes.

The lighting device of the present invention can be used for public treatment purposes in public facilities, public spaces, products for public use, and the like, or can be used for personal treatment purposes in personal facilities, spaces for personal use, products for personal use, and the like.

Furthermore, the present invention may be used in addition to other treatment devices, not exclusively for the illumination device.

Hereinafter, a specific embodiment of the lighting device according to an embodiment of the present invention is described.

Fig. 8 and 9 illustrate an example in which a lighting device according to an embodiment of the present invention is implemented as a product.

Referring to fig. 8, a lighting device according to an embodiment of the present invention includes: a light-emitting device 100 that emits light; a housing 300 that houses the light emitting device 100; a window 210 disposed at an upper portion of the light emitting device; and a fixing member 220 fixing the window 210 and the housing 300.

The housing 300 is not limited as long as it can accommodate and support the light emitting device 100 and supply power to the light emitting device. For example, as shown, the housing 300 may include a main body 310, a power supply 320, a power supply housing 330, and a power connection 340. The power supply device 320 may be received in the power housing 330 to be electrically connected to the light emitting device 100, and may include at least one IC chip. The IC chip may adjust, convert, or control characteristics of power supplied to the light emitting device 100.

The power supply case 330 may receive and support the power supply device 320, and the power supply case 330, in which the power supply device 320 is fixed, may be located inside the main body 310.

The power supply connection part 340 may be disposed at a lower end of the power supply case 330 to be combined with the power supply case 330. Accordingly, the power connection portion 340 may be electrically connected to the power supply device 320 inside the power housing 330 to function as a channel for providing an external power to the power supply device 320.

The light emitting device 100 may include a substrate 20 and first and second light sources 30 and 40 disposed on the substrate 20, and may have a form according to the above-described embodiment. The light emitting device 100 may be provided on the upper portion of the main body 310 and electrically connected to the power supply device 320. The substrate 20 has a shape corresponding to the fixing member 220 on the upper portion of the body 310 so as to be stably fixed to the body 310.

The window 210 may be disposed on the housing 300 in such a manner as to cover the upper portion of the light emitting device 100. The window 210 may be disposed on the light emitting device 100, and may be fixed to the body 310 to cover the light emitting device 100. A lens member 211 may be provided at the window 210 for facilitating diffusion of light from the light emitting device 100. The window 210 may have a light transmissive material, and the shape and light transmittance of the window 210 may be adjusted to adjust the directional characteristic of the lighting device. Therefore, the window 210 can be modified into various shapes according to the purpose of use and application of the lighting device.

The fixing member 220 may be disposed on the upper portion of the window 210 to fasten the window 210, the light emitting device 100, and the body 310 to each other.

The lighting device with the structure can be installed on various phototherapy instruments. Also, a lighting fixture that is mounted on a wall or a ceiling that constitutes a predetermined space (e.g., a chamber) may be used.

The lighting device according to an embodiment of the present invention may be implemented in a form that can be used in real life.

Referring to fig. 9, a lighting device 1000' according to another embodiment of the present invention may include a bracket 530; a light-emitting device 100 that emits light; the reflective cover 400 surrounds the supporting frame 520 and the light emitting device 100. The illumination stand according to another embodiment of the invention may also be arranged on various treatment apparatuses.

An input 530 for controlling the lighting fixture may be disposed on a surface of the bracket 510. The bracket 510 is connected and fixed to the substrate 20 on which the light emitting device 100 is disposed through a support bracket 520. The bracket 510 supplies power to the light emitting device through the power supply part 600. The support bracket 520 may be connected between the bracket 510 and the substrate 20 on which the light emitting device 100 is disposed, and may be provided at the inside thereof with an electric wire (not shown) for supplying power.

In the present embodiment, the support frame 520 is formed of a single rigid member, but is not limited to this, and may be formed of a bendable member that can be bent at least once, or a member having flexibility, and may be changed to various shapes. For example, the support frame 520 may have a degree of flexibility that is deformed by a predetermined degree of external force and maintains its shape without external force. For example, a person may apply an external force to the wiring part 130 to change a part of the shape of the wiring part 130, but in a case where the external force is removed, the wiring part 130 may maintain a final shape after the external force is applied. To this end, the support frame 520 may also be provided in a bellows configuration.

The reflection cover 400 surrounding the light emitting device 100 may be formed using a metal material such as aluminum or the like capable of reflecting a light source emitted from the light emitting device and improving illuminance or may include a material capable of realizing light transmission. A coating layer including a photocatalytic substance may be formed on the inner side surface of the reflection cover 400. The photocatalytic material may include a material derived from TiO₂、ZnO、ZrO₂、WO₃At least one of the group (1).

Hereinafter, an example of testing the sterilization effect of the light irradiation device according to an embodiment of the present invention will be described.

Experimental example 1-Sterilization Effect according to irradiation conditions

Fig. 10 is a graph illustrating a sterilization effect according to irradiation conditions when light is irradiated to a sterilization object using a light emitting device according to the related art invention and a light emitting device according to an embodiment of the present invention. In fig. 10, the bacteria to be sterilized were staphylococcus aureus, which was smeared on a bacterial culture medium, cultured at 35 to 37 ℃ for one day, the bacterial colonies formed on the bacterial culture medium were collected, turbid with physiological saline and centrifuged, and after the supernatant was discarded, the physiological saline was added and diluted to prepare a bacterial solution having a concentration suitable for sterilization experiments. The bacterial solution thus produced was put into a separate container, and the light-emitting device according to the present invention and the light-emitting device according to an embodiment of the present invention were installed at a specific distance from the container and sequentially irradiated with light. Thereafter, the bacterial solution after the completion of the light irradiation was diluted and uniformly applied on a bacterial culture medium, and after one day of cultivation at a temperature of 35 to 37 ℃, colonies formed on the bacterial culture medium were confirmed and counted by multiplying the colonies by the dilution factor, thereby obtaining a result on the sterilization effect.

The x-axis is an axis representing the dose of the first light and the dose of the second light, and the y-axis is an axis representing the degree of inactivation of bacteria on a logarithmic scale. Comparative example 1 is an example in which light of 275nm wavelength band is applied to bacteria as a case in which only the second light is applied to bacteria. Comparative example 2 is an example in which light of 405nm wavelength band is applied to bacteria as a case in which only the first light is applied to bacteria. Comparative example 3 is an example in which the second light of 275nm band was applied to the bacteria, and then the first light of 405nm band was applied. The embodiment is an example in which a first light of 405nm band is applied to bacteria and then a second light of 275nm band is applied thereto. However, in the graph, the case of comparative example 1 was at 3mJ/cm only²The case of applying the 275nm band second light, the case of comparative examples 2, 3 and examples are the case of applying by changing the dose of the 405nm band first light, respectively, that is, at 30J/cm²、60J/cm²、90J/cm²、120mJ/cm²、150J/cm²Applying a first light at a dose of 3mJ/cm²The dose of 275nm band of second light is applied. Wherein, in case of the second light, the dose of the second light is determined to be lower than the dose of the first light in consideration of an allowable dose to a human body.

Referring to FIG. 10, in comparative example 1, the second light was applied to the bacteria at 3mJ/cm²The inactivation ratio of (2) was about 1.5(log CFU/ml) when the dose of (3) was applied, and the case of comparative example 2 was regarded as the case of applying only the first light to the bacteria, when the dose was 30J/cm²When the dose of (a) is administered, the inactivation rate is expressed as about 1(log CFU/ml). As for bacteria at 3mJ/cm²First irradiating the second light at a dose of 30J/cm²The inactivation rate was about 1.5(log CFU/ml) in the case of post-dose irradiation with the first light. However, at 30J/cm²First irradiating the first light at a dose of 3mJ/cm²In the case of the example in which the second light was irradiated after the dose of (c), the inactivation rate was about 4(log CFU/ml), and a very high bactericidal effect was exhibited. Among them, it was confirmed in comparative example 3 and examples that even if only the order of the first light and the second light is different and the same amount of light was irradiated to the bacteriaThe actual sterilization degree also shows significant effect difference.

Also, in the case of comparative examples 2, 3 and examples, the dose of the first light was 60J/cm²The same state was exhibited at the dose of (c), i.e., the bactericidal effect of the example was significantly higher than that of comparative example 2 or comparative example 3.

However, the dose at the first light was 90J/cm²In the above case, comparative example 3 and example showed a stagnant value of about 6(log CFU/ml), which can be judged because bacteria capable of being sterilized no longer exist under laboratory conditions where no new bacteria flowed in. Accordingly, it can be predicted that the sterilization effect of the example is significantly higher than that of comparative examples 1 to 3 under the open external condition in which new bacteria continuously flow in.

Table 2 below shows the minimum dose for obtaining the desired degree of sterilization in comparative examples 1 to 3 and examples. In comparative example 1, as an example in which only the second light was applied to the bacteria, light of 275nm wavelength band was applied to the bacteria. Comparative example 2 as an example of applying only the first light to the bacteria, light of a 405nm band was applied to the bacteria. Comparative example 3 is an example in which the second light of 275nm band was applied to the bacteria, and then the first light of 405nm band was applied. The embodiment is an example in which a first light of 405nm band is applied to bacteria and then a second light of 275nm band is applied thereto.

Referring to table 1, in the case of comparative example 1 using the second light source alone, the sterilization effect of 90% or more, 99% or more, or 99.9% or more was obtained even with a very small dose. However, in the case of the second light, since an image of a human body is large, it is difficult to sterilize only with the second light increment.

Then, it was confirmed that, when comparative examples 2, 3 and 3 in which the first light and the second light were mixed and used were observed, higher bactericidal effects could be obtained with a smaller dose of the first light in the examples than in comparative examples 2 and 3. For example, in order to obtain 99% bactericidal effect, in the case of comparative example 2, 65J/cm is required²The dose of (4) and, in the case of comparative example 3, 40J/cm were required²In contrast, inIn the case of the examples, only 15J/cm are required²The dosage of (a).

[ TABLE 1 ]

As described above, it was confirmed that the light emitting device according to an embodiment of the present invention exhibited a significantly higher sterilization effect than the related art invention.

Experimental example 2-independent bactericidal power test of first and second lights

In the present test, MRSA strains were used as pathogens, and after the MRSA strains were cultured, suspensions of predetermined bacterial concentrations (7log) were prepared. The bacterial suspension is irradiated with a first light and a second light according to the light quantity respectively. In this case, the wavelength of the first light is 405nm, and the wavelength of the second light is 275 nm. The bacteria irradiated with the first light and the second light are diluted to a predetermined concentration, inoculated on an agar plate, and then cultured. Subsequently, the number of colonies of the cultured bacteria was confirmed, and the number thereof was converted to a logarithmic value. Each test was performed five times under the same conditions.

Table 2 and fig. 11a are tables and graphs showing the results of the bactericidal activity test with the first light, and table 3 and fig. 11b are tables and graphs showing the results of the bactericidal activity test with the second light.

[ TABLE 2 ]

Light quantity (J/cm)²)	0	30	60	90	120
						Number of bacteria (log)	7.00	5.97	5.78	5.15	4.17
Error of the measurement	0.00	0.32	0.35	0.43	0.29

Referring to table 2 and fig. 11a, it was confirmed that the number of bacteria decreased as the amount of the first light applied increased. Even when the error range is taken into consideration, the reduction of the number of bacteria is significant.

[ TABLE 3 ]

Light quantity (mJ/cm)²)	0	1	2	3
					Number of bacteria (log)	7.00	6.23	5.88	5.45
Error of the measurement	0.00	0.23	0.27	0.18

Referring to table 3 and fig. 11b, it was confirmed that the number of bacteria decreased as the amount of the second light applied increased. Even when the error range is taken into consideration, the reduction of the number of bacteria is significant. Also, in the case of the second light, it can be known that sterilization is performed in a much smaller amount than the first light.

Experimental example 3-Sterilization test with combination of first and second lights

In the present test, MRSA strains were used as pathogens, and after the MRSA strains were cultured, suspensions of predetermined bacterial concentrations (7log) were prepared. The case where the bacterial suspension was irradiated with the first light alone and the second light alone, and the first light and the second light were irradiated in combination, and no light was irradiated to the bacterial suspension is shown as comparative example 1, the case where the bacterial suspension was irradiated with the second light alone is shown as comparative example 2, the case where the bacterial suspension was irradiated with the first light alone is shown as comparative example 3, and the case where the bacterial suspension was irradiated with the first light and the second light in combination is shown as an example. At this time, the wavelength of the first light was 405nm and the dose was 120J/cm²The second light has a wavelength of 275nm and a dose of 3mJ/cm². In the case of the examples, at 3mJ/cm²After the second light is irradiated, the dose of (2) is 120J/cm²The first light is irradiated. Subsequently, the bacteria of comparative examples 1 to 3 and examples were diluted to a predetermined concentration, inoculated on an agar plate, and then cultured. Subsequently, the cultured bacteria were confirmedAnd (4) falling the number and converting the numerical value into a logarithmic value.

Each test was performed five times under the same conditions.

Fig. 12a and table 4 are a graph and table showing the number of bacteria in the case of irradiating the first light and the second light individually and in the case of irradiating the first light and the second light in combination, respectively, and fig. 12b and table 5 are a graph and table showing the bactericidal activity in the case of irradiating the first light and the second light individually and in the case of irradiating the first light and the second light in combination.

[ TABLE 4 ]

Light conditions	Comparative example 1	Comparative example 2	Comparative example 3	Examples
					Number of bacteria (log)	7.00	5.45	4.17	2.83
Error of the measurement	0.00	0.18	0.29	0.37

[ TABLE 5 ]

Light conditions	Comparative example 1	Comparative example 2	Comparative example 3	Examples
					Sterilizing power	0.00	1.55	2.83	4.17
Error of the measurement	0.00	0.18	0.29	0.37

Referring to fig. 12a, 12b, table 4 and table 5, the second light alone exhibited a bactericidal activity of about 90%, the first light alone exhibited a bactericidal activity of about 99%, and the first light and the second light were combined and irradiated, exhibited a bactericidal activity of 99.99% or more. Thus, it was confirmed that the bacterial count was significantly reduced under the condition of irradiation with the first light and the second light in combination, as compared with the case of not irradiating light and the case of irradiating the first light and the second light alone, and thereby the bactericidal activity was significantly increased.

Experimental example 4-Sterilization Change test according to the Combined sequence of the first light and the second light

At this time, in the case of example 1, the concentration was 3mJ/cm²At a dose of 120J/cm after irradiating 275nm of the second light²The dose of (2) was irradiated with a first light of 405nm, in the case of example 2, at 120J/cm²After irradiating a first light of 405nm at a dose of 3mJ/cm²The dose of (3) was irradiated with 275nm of the second light.

Then, the bacteria of comparative example, example 1 and example 2 were diluted to a predetermined concentration, inoculated on an agar plate, and then cultured. Subsequently, the number of colonies of the cultured bacteria was confirmed, and the number thereof was converted to a logarithmic value.

Each test was performed five times under the same conditions.

Fig. 13a and table 6 are a graph and table showing the number of bacteria irradiated in a case where the combination order of the first light and the second light is set to be different, and fig. 13b and table 7 are a graph and table showing the bactericidal activity irradiated in a case where the combination order of the first light and the second light is set to be different.

[ TABLE 6 ]

Light conditions	Comparative example	Example 1	Example 2
				Number of bacteria (log)	7.00	2.83	0.00
Error of the measurement	0.00	0.37	0.00

[ TABLE 7 ]

Light conditions	Comparative example	Example 1	Example 2
				Sterilizing power	0.00	4.17	7.00
Error of the measurement	0.00	0.37	0.00

Referring to fig. 13a, 13b, table 6 and table 7, example 1 exhibited 99.99% bactericidal activity, whereas example 2 did not show any bacteria, and it was confirmed that substantially complete sterilization was achieved. That is, in the case where the second light is irradiated after the first light is irradiated, significantly higher bactericidal activity is exhibited at the same amount of irradiated light than in the case of the opposite, which means that the same bactericidal activity can be obtained with a smaller amount of light than in the case where the first light is irradiated after the second light is irradiated. Since applying a smaller amount of light means that the light irradiation time is shortened, the case of embodiment 2 can shorten the light irradiation time compared to embodiment 1.

Experimental example 5 setting of light quantity conditions (ex vivo)

Based on the significant increase in bactericidal power exhibited by the sequential irradiation of the first light and the second light, in order to know the optimal light amount for each light source, the number of bacteria and bactericidal power were measured while varying the light amount under the ex-vivo condition when the first light and the second light were sequentially irradiated.

In the present test, MRSA strains were used as pathogens, and after the MRSA strains were cultured, suspensions of predetermined bacterial concentrations (7log) were prepared. Changing the dose of the first light to 30J/cm²、60J/cm²、90J/cm²、120J/cm²The bacterial suspension is irradiated with a first light and a second light in sequence. However, in the case of the second light, 275nm light is limited to a dose of 3mJ/cm2 in consideration of a human body's allowable level.

Then, the bacteria were diluted to a predetermined concentration, inoculated on an agar plate, and then cultured. Subsequently, the number of colonies of the cultured bacteria was confirmed, and the number thereof was converted to a logarithmic value.

Each test was performed five times under the same conditions.

Fig. 14a and table 8 are a graph and table showing the number of bacteria when the first light and the second light are sequentially irradiated and the light quantity of the first light is made different, and fig. 14b and table 9 are a graph and table showing the bactericidal activity when the first light and the second light are sequentially irradiated and the light quantity of the first light is made different.

[ TABLE 8 ]

Light quantity (J/cm)²)	0	30	60	90	120
						Number of bacteria (log)	7.00	3.47	2.13	1.70	0.00
Error of the measurement	0.00	0.13	0.27	0.22	0.00

[ TABLE 9 ]

Light quantity (J/cm)²)	0	30	60	90	120
						Sterilizing power	0.00	3.53	4.87	5.03	7.00
Error of the measurement	0.00	0.13	0.27	0.22	0.00

Referring to FIGS. 14a, 14b, 8 and 9, it was confirmed that the number of bacteria decreased as the light amount of the first light increased, and it was confirmed that the light amount was 120J/cm²Complete sterilization is achieved at a light quantity of (1).

EXAMPLE 6 light quantity Condition setting (in vivo)

In example 4, the dose at the second light (405nm) was 3mJ/cm²Under the condition of (1), when the dose of the first light (275nm) is 120J/cm²When the bacteria were completely sterilized, it was confirmed whether or not the bacteria were sterilized in vivo (invivo) conditionsThe test was also conducted with the bactericidal effect as described above.

In this test, experiments were performed with mice in order to confirm the effectiveness and safety of light application under in vivo (invivo) conditions. The light quantity conditions were the same as those under the ex vivo conditions. When BALB/c mice (6 to 8 weeks old) were used to shave (cut) the back hairs of the mice, wounds of 10mm in diameter were formed on the back. After the above wound was inoculated (inoculated with 5log) of the pathogenic bacteria, the dose of the first light was changed to 30J/cm²、60J/cm²、90J/cm²、120J/cm²And the first light and the second light are irradiated in sequence. However, in the case of the second light, 275nm light is defined as 3mJ/cm in consideration of the human body's allowable level²The dosage of (a). Then, the tissue was collected, and the collected tissue was disrupted, diluted to a predetermined concentration, inoculated onto an agar plate, and cultured. Subsequently, the number of colonies of the cultured bacteria was confirmed, and the number thereof was converted to a logarithmic value.

Each test was performed five times under the same conditions.

Fig. 15a and table 10 are a graph and table showing the number of bacteria when the first light and the second light are sequentially irradiated and the light quantity of the first light is made different, and fig. 15b and table 11 are a graph and table showing the bactericidal activity when the first light and the second light are sequentially irradiated and the light quantity of the first light is made different.

[ TABLE 10 ]

Light quantity (J/cm)²)	0	30	60	90	120
						Number of bacteria (log)	5.00	3.17	3.32	1.48	0.00
Error of the measurement	0.00	0.36	0.38	0.31	0.00

[ TABLE 11 ]

Light quantity (J/cm)²)	0	30	60	90	120
						Sterilizing power	0.00	1.83	1.68	3.52	5.00
Error of the measurement	0.00	0.36	0.38	0.31	0.00

Referring to FIGS. 15a, 15b, 10 and 11, it was confirmed that the number of bacteria was also reduced with the increase of the light amount of the first light under the in vivo (invito) condition, and it was confirmed that the number of bacteria was 120J/cm²Complete sterilization is achieved at a light quantity of (1).

EXAMPLE 7 evaluation of effectiveness 1 (in vivo)

In example 5, the dose of light used for sterilization under in vivo conditions was confirmed, and based on this, the bactericidal power and the change in the number of bacteria under in vivo conditions according to time were tested.

The test was performed with rats. When BALB/c mice (6 to 8 weeks old) were used to shave (cut) the back hairs of the mice, wounds of 10mm in diameter were formed on the back. After the above wound was inoculated (inoculated with 5log) of pathogenic bacteria, the dose of the first light (405nm) was set to 120J/cm²And the first light and the second light were irradiated successively and repeatedly six times a day at the same time. However, in the case of the second light, 275nm light is defined as 3mJ/cm in consideration of the human body's allowable level²The dosage of (a).

Subsequently, in order to confirm the number of bacteria per day, tissues were collected, the collected tissues were disrupted, diluted to a predetermined concentration, inoculated on an agar plate, and then cultured. Subsequently, the number of colonies of the cultured bacteria was confirmed, and the number thereof was converted to a logarithmic value. The amount of bacteria was measured until three times of light irradiation in order to confirm the initial bactericidal activity.

Fig. 16 and table 12 are graphs and tables showing changes in bactericidal activity with date under in vivo conditions, and fig. 17 and table 13 are graphs and tables showing results of measuring the number of bacteria with respect to date under in vivo conditions. In fig. 17 and table 13, the comparative example is a non-irradiation group not irradiated with light, and the example corresponds to a light irradiation group irradiated with light.

[ TABLE 12 ]

[ TABLE 13 ]

Referring to fig. 16, 17, 12 and 13, it was confirmed that the bactericidal activity was maintained at 99.99% or more continuously after the initial stage of the wound irradiation with light, and it was considered that the number of bacteria was substantially close to 0 in the case of the irradiation with light.

EXAMPLE 8 evaluation of effectiveness 2 (in vivo)

In example 5, the dose of light used for sterilization under in vivo conditions was confirmed, and based on this, the wound healing effect by light irradiation under in vivo conditions was tested.

The test was performed with rats. When BALB/c mice (6 to 8 weeks old) were used to shave (cut) the back hairs of the mice, wounds of 10mm in diameter were formed on the back. After the above wound was inoculated (inoculated with 5log) of pathogenic bacteria, the dose of the first light (405nm) was set to 120J/cm²And the first light and the second light were irradiated successively and repeatedly six times a day at the same time.However, in the case of the second light, 275nm light is defined as 3mJ/cm in consideration of the human body's allowable level²The dosage of (a).

Changes in the shape (especially, changes in the area) of the wound were observed at the same time each day. For wound size, values were observed and recorded daily until the epithelialization time point.

Fig. 18 and table 14 are a graph and a table showing changes in wound area with date under in vivo conditions. In fig. 18 and table 14, the comparative example corresponds to a non-irradiation group in which light is not irradiated, and the example corresponds to a light irradiation group in which light is irradiated. Fig. 19a and 19b are photographs of the shape of the area of the wound as the date and time elapses, fig. 19a is a photograph of the wound of the non-irradiation group, and fig. 19b is a photograph of the wound of the irradiation group.

[ TABLE 14 ]

Sky	Inoculation of	0	2	3	6	10	15
								Non-irradiation group	100.0	100.0	108.8	93.8	83.3	55.9	22.4
Error of the measurement	7.8	7.8	7.0	5.0	3.8	2.7	4.2
								Light irradiation group	100.0	100.0	101.0	82.1	50.3	28.8	0.0
Error of the measurement	7.8	7.8	4.1	3.6	1.9	3.2	0.0

Referring to fig. 18, table 14, fig. 19a and fig. 19b, the wound was not visually observed to be healed and the number of bacteria in the wound was significantly reduced by day 2 after the wound appeared, and it was considered as a stage of performing sterilization. The wound healing stage may be considered to be a stage in which scabs are formed from day 2 after the wound has appeared and then the area of the wound is gradually reduced from day 2 after the wound has appeared. If scabbing occurs in the wound, the wound is not exposed to the outside due to the scabbing, and additional infection is reduced every week. However, there is a great difference in the size of the scab and the recovery of the wound depending on whether or not sterilization is performed before the scab is formed. In the wound healing phase, the time point at which the area of the wound was reduced to 50% was no more than 6 days in the case of the irradiated group, whereas 10 days were required in the case of the non-irradiated group. In the case of the light irradiation group, epithelialization occurred on day 15, but in the case of the non-irradiation group, epithelialization did not occur on day 15. By this, according to an embodiment of the present invention, it was confirmed that the wound healing effect was significant when the light was irradiated

EXAMPLE 9 safety evaluation 1 (in vivo)

In order to confirm whether the irradiation conditions in the above experimental examples were harmful to human bodies, it was confirmed whether DNA was mutated.

In the present test, in order to confirm whether or not uninfected tissues have DNA mutation (mutation) due to light irradiation, the degree of formation of thymine dimers (thymine dimers) after light irradiation was confirmed by immunohistochemical analysis (immunohistochemical analysis). When UV is excessively irradiated to DNA, DNA such as thymine dimer is mutated to cause cell death, and whether DNA is mutated or not can be confirmed by the degree of formation of thymine dimer.

The test was performed with rats. The rats were BALB/c rats (6-8 weeks old), and after shaving the back hairs of the rats, wounds having a diameter of 10mm were formed on the back by a punch. The wound was irradiated with light, and then a tissue was collected, and the collected tissue was fixed with formalin and paraffin, and then a section was taken. When light was irradiated, the control group was a non-irradiated group which was not subjected to light treatment, the experimental group 1 was a light-irradiated group which was treated with an excessive amount of UVC, and the experimental group 2 was a group in which the dose of the first light (405nm) was limited to 120J/cm²And the dose of the second light (275nm) was limited to 3mJ/cm²And the light irradiation groups are sequentially irradiated.

Fig. 16a and table 15 are graphs and tables showing the content of thymine dimers in the tissues as a percentage. Referring to fig. 20a and table 15, thymine dimer was found in experimental group 1, whereas thymine dimer was not found in experimental group 2. Accordingly, it was confirmed that the light conditions applied to the embodiment of the present invention did not cause DNA mutation even when uninfected tissue was irradiated.

[ TABLE 15 ]

	Control group	Experimental group 1	Experimental group 2
				Content (%)	2	58	3
Error of the measurement	1	8	1

EXAMPLE 10 evaluation of safety 2 (in vivo)

Whether ROS were produced was confirmed in order to confirm whether the irradiation conditions in the above experimental examples were harmful to humans.

This test was used to confirm whether Reactive Oxygen Species (ROS) were also induced by light irradiation in uninfected tissues. When the infectious bacteria are irradiated with germicidal light, ROS is induced to cause the bacteria to die.

The test was performed with rats. The rats were BALB/c rats (6-8 weeks old), and after shaving the back hairs of the rats, wounds having a diameter of 10mm were formed on the back by a punch. After the wound was irradiated with light, dichlorofluorescein diacetate (DCFH-DA) was treated at the irradiated site, and the amount of luminescence (emission) in the portion stained by DCFH-DA was measured to confirm the presence or absence of ROS. DCFH-DA is oxidized intracellularly by ROS and fluoresces. The absorption wavelength of DCFH-DA excitation is 445nm to 490nm, and the fluorescence emission wavelength is 515nm to 575 nm.

The control group was a non-treated group without any additional treatment, the test group 1 was a hydrogen peroxide-treated group, and the test group 2 was a group in which the dose of the first light (405nm) was limited to 120J/cm²And the dose of the second light (275nm) was limited to 3mJ/cm²And sequentially irradiating the treatment groups.

FIG. 20b and Table 16 are graphs and tables showing the degree of tissue luminescence stained by DCFH-DA. Referring to fig. 20b and table 16, in experimental group 2, fluorescence occurred in experimental group 1 and the presence of ROS was confirmed, whereas in experimental group 2, fluorescence did not occur and ROS was judged to be absent. Accordingly, it was confirmed that no ROS are generated even if the light conditions applied to an embodiment of the present invention are irradiated to uninfected tissues.

[ TABLE 16 ]

Although the present invention has been described with reference to the preferred embodiments, it is to be understood that various modifications and alterations can be made by those skilled in the art or those having the basic knowledge in the art without departing from the spirit and scope of the present invention as set forth in the claims. Therefore, the technical scope of the present invention is not limited to the details described in the detailed description, but should be determined only by the scope described in the claims.

34页详细技术资料下载

上一篇：一种医用注射器针头装配设备

下一篇：用于对组织进行选择性治疗的装置

Light irradiation device

相关技术

网友询问留言