阅读说明：本技术 一种快速测定cgrp/cgrp受体抗体药物生物学活性的方法 (Method for rapidly determining pharmaceutical biological activity of CGRP/CGRP receptor antibody ) 是由 王军志 王兰 于传飞 付志浩 郭潇 黄璟 刘春雨 段茂芹 郭莎 于 2020-11-24 设计创作，主要内容包括：本发明公开了一种快速测定CGRP/CGRP受体抗体药物生物学活性的方法。所述方法包括构建得到稳定表达CRE报告基因的效应细胞,用CGRP刺激激活报告基因表达,并用CGRP/CGRP受体抗体药物阻断CGRP信号通路,根据测定的报告基因信号值拟合四参数曲线确定抗体的生物学活性。本发明针对CGRP/CGRP受体抗体药物生物学活性的测定首次建立了一种快速、准确的定量检测方法,所述方法能够准确、简单、快速地检测CGRP/CGRP受体抗体药物的生物学活性。(The invention discloses a method for rapidly determining the biological activity of a CGRP/CGRP receptor antibody medicament. The method comprises the steps of constructing effector cells for stably expressing CRE reporter genes, stimulating and activating the reporter gene expression by using CGRP, blocking a CGRP signal channel by using CGRP/CGRP receptor antibody drugs, and fitting a four-parameter curve according to the measured signal values of the reporter genes to determine the biological activity of the antibody. The invention establishes a rapid and accurate quantitative detection method for the determination of the biological activity of the CGRP/CGRP receptor antibody medicament for the first time, and the method can accurately, simply and rapidly detect the biological activity of the CGRP/CGRP receptor antibody medicament.)

1. A method for constructing a cell strain for rapidly determining the pharmaceutical biological activity of a CGRP/CGRPR antibody is characterized by comprising the following steps:

(1) transducing effector cells with a plasmid containing a CRE-Luc reporter gene;

(2) screening to obtain a cell strain for stably expressing the reporter gene;

preferably, the effector cell is a human neuroma cell;

more preferably, the effector cell is SK-N-MC.

2. A method for rapidly determining the pharmaceutical biological activity of CGRP/CGRPR antibodies, which is characterized by comprising the following steps:

(1) constructing effector cells stably expressing a CRE-Luc reporter gene according to the method of claim 1;

(2) adding CGRP to the effector cells of step (1) to stimulate expression of an activation reporter gene;

(3) diluting the CGRP/CGRPR antibody drug sample and the reference substance in equal proportion, respectively transferring to the mixture of the effect cells and the CGRP in the step (2), and incubating in an incubator at 37 ℃;

(4) adding a luciferase substrate to the step (3), and fitting a four-parameter curve according to the measured reporter gene signal value to determine the biological activity of the CGRP/CGRPR antibody drug sample.

3. The method according to claim 2, wherein the effector cells in step (1) have a cell density of 5 x 10³-4×10⁴One/hole, preferably 1X 10⁴Per well.

4. The method of claim 2, wherein the final concentration of the CGRP in step (2) is 0.4ng/mL to 5ng/mL, preferably 5 ng/mL.

5. The method according to claim 2, wherein the proportional dilution in step (3) is in a ratio of 1:1.8 to 1:2, preferably 1: 2.

6. The method according to claim 2, wherein the incubation in step (3) is for a period of 2-22 hours, preferably 4 hours.

7. The method of claim 2, wherein the biological activity of the sample in step (4) is determined by comparing the half inhibitory concentration values of the four parameter curve of the sample to a reference to obtain the relative potency of the sample.

8. A product for rapidly determining the pharmaceutical biological activity of CGRP/CGRPR antibodies, which is characterized in that the product comprises a cell strain stably expressing a CRE-Luc reporter gene.

9. A system for the evaluation of the pharmaceutical biological activity of CGRP/CGRPR antibodies, comprising the following components:

(1) a cell strain stably expressing a CRE-Luc reporter gene;

(2) CGRP, CGRP/CGRPR antibody drug samples and reference substances;

(3) a luciferase substrate.

10. Use of the system of claim 9 for quality control of CGRP/CGRPR antibody drugs;

preferably, the application comprises the method of claim 2.

Technical Field

The invention belongs to the field of biomedical detection, and particularly relates to a method for rapidly determining the biological activity of a CGRP/CGRP receptor antibody medicament.

Background

Migraine is the third most common and sixth most disabling disease worldwide and is a common chronic neurovascular disease characterized by recurrent episodes of intense headache, mostly hemifacial. It is estimated that the total number of migraine sufferers is more than 10 hundred million people worldwide, with about 90% being Episodic Migraine (EM), characterized by migraine days of up to 14 days per month; the remaining 10% are chronic migraine headaches (CM) characterized by headache episodes of at least 15 days per month, with migraine headaches occurring on 8 and above days, and a patient's duration of illness exceeding 3 months. At present, no medicine can cure migraine. The World Health Organization (WHO) has listed migraine as one of the 10 most disabling diseases, and migraine sufferers are more likely to suffer from depression, anxiety, sleep disorders, other pain and fatigue, etc. than others. CGRP (calcitonin gene-related peptide) is a neuropeptide that has been shown to be released during migraine attacks, and may be a cause of migraine attacks. At present, CGRP and its receptor (CGRPR) have become the hot target for the development of migraine drugs.

The antibody drug becomes one of important components in the field of biological medicine due to the unique action mechanism and high efficiency, has wide application prospect in disease treatment, and has been successfully applied to the treatment of various diseases such as tumors, autoimmune diseases, infectious diseases, transplant rejection and the like. In 2017, 69 monoclonal antibody drugs are approved by the FDA and sold on the market, including fully human monoclonal antibodies, humanized monoclonal antibodies, chimeric monoclonal antibodies, murine monoclonal antibodies, antibody conjugate drugs, bispecific antibodies and antibody analogs, and at present, autoimmune diseases and cancers still remain the main application fields of monoclonal antibody drugs. The CGRP/CGRPR antibody is a CGRP/CGRPR blocker and is used for treating migraine, and no related medicine is on the market at present in China. The CGRP/CGRPR antibody is combined with a CGRP receptor or a soluble CGRP on a cell membrane, and the combination of the CGRP and the CGRP receptor is blocked, so that a CGRP signal path cannot be activated, thereby inhibiting related mechanisms and effectively improving symptoms of migraine.

In the face of the rapid development of antibody drugs, the establishment of a corresponding antibody drug quality evaluation and quality control technical system is urgently needed. The biological activity measurement is the measurement of the effective components and content of the medicine and the potency of the medicine, and is an important quality control index for ensuring the effectiveness of the antibody medicine. At present, no method for measuring the biological activity of the CGRP/CGRPR antibody exists in China, the method adopts a transgenic cell activity measuring method to measure the biological activity of the CGRP/CGRPR antibody, the result can be obtained in 4 hours, the experimental period is short, the operation is simple and convenient, the problem of objective factors such as cell pollution possibility caused by long-time incubation is solved, and the method for measuring the biological activity of the CGRP/CGRPR antibody medicament is provided for the first time.

Disclosure of Invention

In order to make up for the defects in the prior art, the invention aims to provide a method for rapidly determining the biological activity of a CGRP/CGRPR antibody drug, the method has a short experimental period, the result can be obtained in 4 hours, the operation is simple and convenient, the problem of objective factors such as the possibility of cell contamination caused by long-time incubation is solved, and the method for determining the biological activity of the CGRP/CGRPR antibody drug is provided for the first time.

The above object of the present invention is achieved by the following technical solutions:

the invention provides a method for constructing a cell strain for rapidly determining the pharmaceutical biological activity of a CGRP/CGRPR antibody.

Further, the method comprises the steps of:

(1) transducing effector cells with a plasmid containing a CRE-Luc reporter gene;

(2) and screening to obtain the cell strain stably expressing the reporter gene.

Further, the effector cells in step (1) are preferably human neuroma cells;

more preferably, the effector cell is SK-N-MC.

The SK-N-MC cell belongs to a human neuroepithelial tumor cell, the surface of the SK-N-MC cell expresses a CGRP receptor (heterodimer consisting of a calcitonin-like receptor (CLR) and a receptor activity modified protein 1(RAMP 1)), the CGRP is combined with the CGRP receptor, downstream adenylate cyclase is activated, the intracellular cAMP level is increased, and the cell nucleus recognizes and combines a CRE sequence, so that the function is generated. The Plv-CRE-Luc-PGK-blisidin plasmid which is constructed synthetically is packaged by slow virus, and then is transferred into SK-N-MC cells, CGRP is combined with CGRP receptor to activate cAMP signal path, and the expression of luciferase reporter gene connected with CRE sequence is started. anti-CGRP (CGRPR) antibodies compete with CGRP (CGRPR) for binding to CGRPR (CGRP) and block the CGRP signaling pathway, and therefore, the concentration of anti-CGRP/CGRPR antibody is inversely proportional to the amount of luciferase expressed in the effector cells.

Further, the screening described in step (2) is carried out by adding antibiotics.

Further, the antibiotics include (but are not limited to): blasticidin (Blasticidin), Hygromycin (Hygromycin B), Puromycin (Puromycin), 6-thioguanine (6-thioguanine), Ampicillin (Ampicillin);

preferably, the antibiotic is Blasticidin (Blasticidin).

As a preferred embodiment of the invention, the plasmid of the luciferase reporter gene containing the CRE sequence is Plv-CRE-Luc-PGK-BLASTICIdin plasmid, after the plasmid is introduced into SK-N-MC effector cells, Blasticidin (Blasticidin) is added for screening, and a monoclonal cell strain for stably expressing the CRE-Luc reporter gene is obtained.

In a second aspect, the invention provides a method for rapidly determining the pharmaceutical biological activity of CGRP/CGRPR antibodies.

Further, the method comprises the steps of constructing effector cells for stably expressing the CRE-Luc reporter gene, stimulating and activating the reporter gene expression by using CGRP, blocking a CGRP signal channel by using CGRP/CGRPR antibody drugs, and fitting a four-parameter curve according to the measured signal value of the reporter gene to determine the biological activity of the antibody drugs.

Further, the method comprises the steps of:

(1) according to the method of the first aspect of the invention, effector cells which stably express a CRE-Luc reporter gene are constructed;

(2) adding CGRP to the effector cells of step (1) to stimulate expression of an activation reporter gene;

(3) diluting the CGRP/CGRPR antibody drug sample and the reference substance in equal proportion, respectively transferring to the mixture of the effect cells and the CGRP in the step (2), and incubating in an incubator at 37 ℃;

(4) adding a luciferase substrate to the step (3), and fitting a four-parameter curve according to the measured reporter gene signal value to determine the biological activity of the CGRP/CGRPR antibody drug sample.

Further, the cell density of the effector cells in the step (1) is 5X 10³-4×10⁴One/hole, preferably 1X 10⁴Per well.

Further, the final concentration of the CGRP in the step (2) is 0.4ng/mL to 5ng/mL, preferably 5 ng/mL.

Further, the proportion of the equal proportion dilution in the step (3) is 1:1.8-1:2, and preferably 1: 2.

Further, the incubation time in step (3) is 2-22 hours, preferably 4 hours.

Further, the four-parameter curve in the step (4) is an inverted S-shaped four-parameter curve fitted by data processing according to the relative chemiluminescence unit values read on the microplate reader using chemiluminescence.

Further, the biological activity of the sample in the step (4) is determined by comparing the half inhibitory concentration values of the four-parameter curve of the sample and the reference substance to obtain the relative potency of the sample.

In the examples of the present invention, chemiluminescence values are measured using a luciferase kit, preferably using the Bright-Glo luciferase kit from Promega corporation.

Luciferase (Luciferase) is a generic term for a class of enzymes that are derived from organisms capable of emitting light in nature and that catalyze the oxidative emission of Luciferin (Luciferase) or fatty aldehydes in the organism. Luciferases can be classified into Firefly Luciferase (FL) and Bacterial Luciferase (BL) depending on the species of the organism from which they are derived. Currently, the luciferase gene derived from firefly in North America is most widely used, and the gene can code for a luciferase protein producing 550 amino acids. FL gene is from firefly, in Mg²⁺、ATP、O₂In the presence of (2), the D-luciferin (D-luciferin) is catalyzed to be subjected to oxidative decarboxylation to generate the activated oxyluciferin, and photons are released to generate fluorescence of 550-580 nm.

Luciferase reporter gene detection is an important tool for analyzing the interaction relationship between potential cis-elements (such as promoters, enhancers, silencers and the like) and trans-acting factors in the flanking region of a structural gene in the field of modern molecular biology research. The luciferase reporter gene system is a reporter system for detecting the activity of firefly luciferase (firefly luciferase) by using Luciferin (luciferase) as a substrate. Luciferase catalyses the oxidation of Luciferin to Oxyluciferin, which in turn fluoresces biologically (Bioluminescence) and is then measured by a chemiluminometer or a scintillation counter.

In an embodiment of the invention, an inverted S-shaped four-parameter curve was fitted by data processing using chemiluminescence read Relative chemiluminescence unit values (RLU) on a microplate reader. The inverse S-shaped four-parameter curve can reflect indexes such as upper and lower asymptotes, IC50 values, slopes and the like. Preferably, the data processing is performed using graphpad7.0 to fit an inverse S-shaped four-parameter curve.

In the examples of the present invention, the relative potency of the samples was obtained by comparing the Half inhibition concentration values (IC 50) of the four parameter curves of the samples with the reference. The calculation formula is as follows: relative potency is reference IC 50/sample IC50 × 100%.

In the embodiment of the invention, the CGRP/CGRPR antibody medicament is an antibody medicament which is developed by Junzhi organisms and aims at CGRP or CGRP receptors. In addition, in order to verify the specificity of the method of the present invention, in the embodiment of the present invention, monoclonal antibody drugs with different target points are used for verification, including Denosumab (target point is RANKL), Trastuzumab (target point is HER2), Infliximab (Infliximab, target point is TNF- α), and weibull's-bit (target point is CD 30).

The third aspect of the invention provides a product for rapidly determining the pharmaceutical biological activity of the CGRP/CGRPR antibody.

Further, the product comprises a cell strain which stably expresses a CRE-Luc reporter gene.

Furthermore, the product also comprises CGRP, CGRP/CGRPR antibody drug samples and reference substances.

Further, the product also includes a luciferase substrate.

In a fourth aspect, the invention provides a system for the evaluation of the pharmaceutical biological activity of CGRP/CGRPR antibodies.

Further, the system comprises the following components:

(1) a cell strain stably expressing a CRE-Luc reporter gene;

(2) CGRP, CGRP/CGRPR antibody drug samples and reference substances;

(3) a luciferase substrate.

The fifth aspect of the invention provides the use of the system according to the fourth aspect of the invention for quality control of CGRP/CGRPR antibody drugs;

preferably, the application comprises the method of the second aspect of the invention.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Further, some terms are explained as follows:

the term "antibody" as used herein includes whole antibodies and any antigen-binding fragment (i.e., "antigen-binding portion") or single chain thereof. An "antibody" is a protein comprising at least two heavy chains (H) and two light chains (L) or antigen-binding portions thereof, which are linked to each other by disulfide bonds. Each heavy chain consists of a heavy chain variable region (VH) and a heavy chain constant region (CH). The heavy chain constant region consists of three domains, CH1, CH2, and CH 3. Each light chain consists of a light chain variable region (VL) and a light chain constant region (CL). The light chain constant region consists of one domain CL. The VH and VL regions can be further subdivided into regions of hypervariability, termed Complementarity Determining Regions (CDRs), interspersed with regions that are more conserved, termed Framework Regions (FRs). Each VH and VL is composed of three CDRs and four FRs arranged from amino-terminus to carboxy-terminus in the following order: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR 4. The variable regions of the heavy and light chains contain binding domains that interact with antigens. Wherein the term "antibody" refers to an immunoglobulin or a fragment or derivative thereof, and includes any polypeptide comprising an antigen binding site, whether produced in vitro or in vivo. The term includes, but is not limited to, polyclonal, monoclonal, monospecific, multispecific, nonspecific, humanized, single-chain, chimeric, synthetic, recombinant, hybrid, mutated, and grafted antibodies. Furthermore, the term "antibody" also includes antibody fragments that retain antigen binding function, such as Fab, F (ab')2, Fv, scFv, Fd, dAb, and other antibody fragments.

The term "sample" as used herein refers to a sample to be tested that is to be identified and assayed. In the present invention, the antibody drug sample includes an antibody drug sample to be tested for biological activity thereof, which is produced in industrial production, and specifically includes CGRP mab, Denosumab (Denosumab), Trastuzumab (Trastuzumab), Infliximab (Infliximab), and weibull's-ituximab (Brentuximab).

The invention has the following advantages and beneficial effects:

(1) the invention provides a method for rapidly determining the pharmaceutical biological activity of a CGRP/CGRP receptor antibody for the first time, and no report is found at home about the determination method of the pharmaceutical biological activity of the CGRP/CGRP receptor antibody at present.

(2) The method for rapidly determining the biological activity of the CGRP/CGRP receptor antibody medicament provided by the invention has the advantages of simple and convenient operation, short experimental period, stable and reliable result, good specificity, high accuracy and the like, can obtain the result in 4h, is low in cost, does not need animal experiments, is favorable for promoting the quality control and clinical application of the CGRP/CGRP receptor antibody medicament, and has good popularization and application values.

(3) The invention provides a construction method of a cell strain for rapidly determining the pharmaceutical biological activity of a CGRP/CGRPR antibody, and a product and a system containing the cell strain, wherein the product and the system can be used for rapidly detecting the pharmaceutical biological activity of the CGRP/CGRPR receptor antibody, and have the advantages of high accuracy, safety and rapidness.

Drawings

Embodiments of the invention are described in detail below with reference to the attached drawing figures, wherein:

FIG. 1 is a graph of the results of an effector cell luciferase activity assay of different clones;

FIG. 2 is a graph of four parameters of SK-N-MC-CRE-Luc cells at different incubation times;

FIG. 3 is a graph of four parameters of SK-N-MC-CRE-Luc cells at different cell densities;

FIG. 4 is a four parameter plot of SK-N-MC-CRE-Luc cells at different antigen concentrations;

FIG. 5 is a four parameter graph of the effect of antibodies on SK-N-MC-CRE-Luc cells at different antigen concentrations;

FIG. 6 is a four parameter graph of the effect of the antibody in SK-N-MC-CRE-Luc cells at different antigen concentrations and different antibody dilution ratios;

FIG. 7 is a graph showing the effect of SK-N-MC-CRE-Luc cells on different target monoclonal antibody drugs.

Detailed Description

The present invention is further illustrated below with reference to specific examples, which are intended to be illustrative only and are not to be construed as limiting the invention. As will be understood by those of ordinary skill in the art: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents. The following examples are examples of experimental methods not indicating specific conditions, and the detection is usually carried out according to conventional conditions or according to the conditions recommended by the manufacturers.

EXAMPLE 1 selection of SK-N-MC cell lines stably expressing CRE-luciferase

1. Experimental Material

SK-N-MC cells are derived from ATCC; the Plv-CRE-Luc-PGK-BLASTIDIdin plasmid was purchased from Ghan King-rui bioengineering, Inc.; CGRP is available from Qiaozhou Biotechnology, Inc.; CGRP antibodies were purchased from Jungguan biomedical science and technology Limited, Suzhou (corresponding to Junggui organisms in the summary of the invention); the Bright-Glo luciferase kit was purchased from Promega.

2. Lentiviral transduction

SK-N-MC cells were transduced by lentivirus packaging method, incubated for 48h, and then cell culture medium containing 5. mu.g/mL of blicidin was added for pressurized selection. After the cell density and viability are restored, the monoclonal was screened in a 96-well plate by limiting dilution at a density of 0.4-0.6/well. During the cell growth period, observing and marking which wells are monoclonal, transferring the cells in the wells to a 24-well plate when the confluency of SK-N-MC cells in the monoclonal wells reaches more than 30%, and gradually expanding and culturing.

3. SK-N-MC-CRE-Luc cell strain screening

10000 cells per well, 100. mu.L per well plated in 96-well plates at 37 ℃ with 5% CO₂The culture was performed overnight, and the following day CGRP was diluted to 6ng/mL, 3ng/mL, 1.5ng/mL, 0.75ng/mL, 0ng/mL (final concentrations were 2ng/mL, 1ng/mL, 0.5ng/mL, 0.25ng/mL, 0ng/mL), 50. mu.L per well, and after 4h 100. mu.L of Bright-Glo was added per well and chemiluminescence was measured.

4. Results of the experiment

The results are shown in Table 1, and after screening, SK-N-MC-CRE-Luc 1A8 cells have better luciferase expression reactivity to CGRP stimulation (see figure 1), so the SK-N-MC-CRE-Luc 1A8 cells are selected as cells for testing and are named as SK-N-MC-CRE-Luc cells.

TABLE 1 SK-N-MC-CRE-Luc clonal screening of chemiluminescence values

Concentration (ng/mL)	0	0.25	0.5	1	2	Signal to noise ratio
							1A8	12162	443032	427805	435885	418060	34
2B6	1272	29629.5	28132.5	32696	38474	30
							4C3	5597	165302	153817	152508	148764	27
6C3	5917	149782	140934	150925	164771	28
							4D6	9623	304126	282649.5	258124	302393	31
1B7	4156	90726	74081	79351	86146	21
							1C8	456	12331	12121	12707	14391	32
4E6	169	5131	4661	4621	4438	26
							2C7	3622	77693	72002	70223	67585	19
1C3	31015	711184	691374	643107	705922	23

Example 2 optimization of the detection method

1. Optimization of CGRP concentration, incubation time, different cell densities

The CGRP concentration is diluted to a higher concentration of 100ng/mL (final concentration), 1:3 is diluted, 11 concentration points are adopted, and the action range of the CGRP is determined according to a four-parameter curve fitted by the measured chemiluminescence values under different cell densities and incubation times, and the appropriate incubation time and cell density are determined.

The experimental method comprises the following steps: SK-N-MC-CRE-Luc cells at 5X 10³-4×10⁴Adding into 96-well white plate at 37 deg.C with 5% CO₂Culturing overnight; CGRP initial concentration 100ng/mL (final concentration), 1:3 dilution 11 concentration gradient, total 12 concentration points (including concentration point 0ng/mL), 37 ℃, 5% CO₂And (5) culturing. Adding Bright-Glo at 2h, 4h, 6h, and 22h respectively, and detecting chemiluminescence value, rootAnd determining a proper incubation time and cell density according to a four-parameter curve fitted with the measured chemiluminescence values and the signal-to-noise ratio.

The experimental results are as follows: the results show that 1X 10⁴The four-parameter curve fitted with chemiluminescence values after 4h incubation at cell density per well and signal-to-noise ratio were good (see fig. 2 and 3), and were suitable as optimal cell density and incubation time; the four-parameter curve obtained by SK-N-MC-CRE-Luc cells under different CGRP concentration stimulation is shown in figure 4.

2. CGRP concentration optimization

According to the experimental results of example 1, CGRP was set to six different concentrations of 0.4ng/mL, 0.6ng/mL, 0.8ng/mL, 1ng/mL, 2.5ng/mL, and 5ng/mL (final concentration), and inhibition experiments of CGRP monoclonal antibody were performed to determine the concentration of CGRP used (final concentration) based on a four-parameter curve fitted to the chemiluminescence values measured.

The experimental method comprises the following steps: SK-N-MC-CRE-Luc cells at 1X 10⁴Adding into 96-well white plate at 37 deg.C with 5% CO₂Culturing overnight; the initial concentration of the CGRP monoclonal antibody is 1200ng/mL (final concentration), the CGRP monoclonal antibody is diluted by 12 concentration points in a 1:3 gradient way and added into the cell plate; CGRP is respectively diluted to 0.4ng/mL, 0.6ng/mL, 0.8ng/mL, 1ng/mL, 2.5ng/mL and 5ng/mL (final concentration), and is respectively transferred to the cell plate; 37 ℃ and 5% CO₂And (5) culturing. After 4h, Bright-Glo was added and the chemiluminescence was measured.

The experimental results are as follows: the results show that the CGRP concentrations at 2.5ng/mL and 5ng/mL (final concentrations) are able to achieve very good signal-to-noise ratios (see FIG. 5).

3. Optimization of antibody dose-effect range and dilution ratio

According to the experimental results of example 2, the CGRP mab was diluted to a higher concentration of 500ng/mL (final concentration), 11 concentration points were diluted at a dilution ratio of 1:2 and 1:1.8, respectively, and the range of action of the CGRP mab was determined according to a four-parameter curve fitted to the measured chemiluminescence values.

The experimental method comprises the following steps: SK-N-MC-CRE-Luc cells at 1X 10⁴Adding into 96-well white plate at 37 deg.C with 5% CO₂Culturing overnight; CGRP diluted to 2.5ng/mL and 5ng/mL (final concentrations)) Transferring into the cell plate; diluting the CGRP monoclonal antibody to 1000ng/mL (final concentration), diluting 11 concentration points according to the dilution ratio of 1:2 and 1:1.8 respectively, and transferring 50 mu L/hole of the diluted antibody into a cell plate; 37 ℃ and 5% CO₂And (5) culturing. After 4h, Bright-Glo was added and the chemiluminescence was measured.

The experimental results are as follows: the results are shown in FIG. 6, according to IC₅₀And calculating values and upper and lower platforms, determining the final concentration of the starting point of the CGRP monoclonal antibody to be 500ng/mL, diluting the CGRP monoclonal antibody by 1:2, and setting ten concentration points, wherein the upper and lower platforms can be ensured to have two concentration points respectively, and the linear part has at least three points.

Example 3 verification of the detection method

The method is a biological activity method aiming at the anti-CGRP/CGRPR antibody, so that the specificity verification of the method adopts monoclonal antibody medicaments with different target points: CGRP (CGRP) monoclonal antibody (CGRP with the target point being RANKL), Denosumab (RANKL with the target point being RANKL), Trastuzumab (Trastuzumab with the target point being HER2), Infliximab (Infliximab with the target point being TNF-alpha) and weibull-tuximab (Brentuximab with the target point being CD 30). Under the same experimental conditions, the SK-N-MC-CRE-Luc reporter gene is used for testing a living system, and the activity of monoclonal antibody drugs with different target points is tested, so that the specificity of the detection method is verified.

The experimental method comprises the following steps: SK-N-MC-CRE-Luc cells at 1X 10⁴Adding into 96-well white plate at 37 deg.C with 5% CO₂Culturing overnight; CGRP was added at 5ng/mL (final concentration); respectively diluting the monoclonal antibody medicaments of different targets to initial concentration of 500ng/mL (final concentration), diluting 10 concentration gradients at a ratio of 1:2, and respectively transferring 50 mu L/hole to the cell plate; 37 ℃ and 5% CO₂And (5) culturing. After 4h, Bright-Glo was added and the chemiluminescence was measured.

The experimental results are as follows: the experimental results are shown in FIG. 7, and the results show that the method has no dose-effect curve for the antibody drugs of the RANKL, HER2, TNF-alpha and CD30 targets, which indicates that the method is not suitable for other drugs except for CGRP/CGRPR targets, and proves that the specificity of the method is better.

The above description of the embodiments is only intended to illustrate the method of the invention and its core idea. It should be noted that, for those skilled in the art, without departing from the principle of the present invention, several improvements and modifications can be made to the present invention, and these improvements and modifications will also fall into the protection scope of the claims of the present invention.