阅读说明：本技术 一种水稻蛋白OsSWC4在调控水稻株型中的用途 (Application of rice protein OsSWC4 in regulation and control of rice plant type ) 是由 丁勇 王世亮 阿齐兹 于 2020-04-10 设计创作，主要内容包括：本发明涉及一种水稻蛋白OsSWC4在调控水稻株型中的用途。本发明还涉及调控水稻株型的方法,其包括使OsSWC4蛋白表达降低的步骤。OsSWC4蛋白表达降低能够降低水稻的株高和减少水稻的分蘖。(The invention relates to application of rice protein OsSWC4 in regulation and control of rice plant types. The invention also relates to a method for regulating the plant type of rice, which comprises the step of reducing the expression of OsSWC4 protein. The reduction of the OsSWC4 protein expression can reduce the plant height of rice and reduce the tillering of the rice.)

1. rice protein or a polypeptide as shown in SEQ ID NO: 7 in the rice plant type regulation, the protein is OsSWC4 protein, and the amino acid sequence of the protein is shown as SEQ ID No: 2, respectively.

2. The use of claim 1, wherein the nucleotide coding sequence of the OsSWC4 protein is as set forth in SEQ ID No: 1 is shown.

3. The use according to claim 1 or 2, wherein the rice plant type is plant height and/or tillering.

4. The use according to any one of claims 1 to 3, wherein the regulation of the plant type of rice is reduction of the plant height of rice and/or reduction of the number of tillers of rice.

5. The use according to any one of claims 1 to 4, wherein the variety of rice is Nipponbare or 9311, preferably Nipponbare.

6. A method for regulating the plant type of rice, comprising the step of reducing the expression of OsSWC4 protein, wherein the amino acid sequence of the OsSWC4 protein is SEQ ID No: 2, wherein the rice variety is, for example, Nipponbare or 9311, preferably Nipponbare.

7. The method of claim 6, wherein the rice plant type is plant height and/or tillering.

8. The method of claim 6 or 7, wherein the modulating the plant type of rice is reducing the plant height of rice and/or reducing the number of tillers of rice.

9. The method of any one of claims 6-8, wherein the step of reducing expression of OsSWC4 protein is accomplished by gene knock-out or gene knock-down methods.

10. The method of claim 9, wherein the gene knockout or gene knockdown method is a method of RNA interference.

11. The method of claim 10, wherein the method of RNA interference is performed using a nucleic acid sequence as set forth in SEQ id no: 7 for RNA interference.

Technical Field

The invention relates to the field of genetic engineering, in particular to a rice plant height and tillering related protein OsSWC4 and a coding gene thereof.

Background

Rice (Oryza sativa L.) is a staple food that provides food for half of the world's population, and approximately more than 50% of the human main source of nutrition is rice^[1]. 95% of the world's rice production and consumption is in Asia, accounting for 40-80% of Asian dietCalories of^[2]. Since 1960, the rapid growth of the population and the drastic reduction of the cultivation area have made it increasingly difficult to meet the food demand, resulting in a global food crisis and thus also opening the green revolution of crops.

In modern agriculture, plant height is an important trait determining grain yield^[3]. The green revolution of the rice has positive influence on the yield potential of the rice, and the breeding of dwarf varieties is taken as a representative^[4]. The rice semi-dwarf gene sd-1 plays an important role in modern rice breeding, and semi-dwarf plants can effectively prevent lodging and increase the utilization rate of nitrogen fertilizer^[5，6]。

Tillering is also an important agronomic character affecting rice yield, and tillering number is a premise for determining spike number and is an important basis for forming yield. Proper amount of effective tillering is more beneficial to high yield of rice. Therefore, the gene research for controlling the plant height and tillering of the rice is beneficial to the improvement of rice varieties.

Disclosure of Invention

The invention provides a gene related to rice tillering and plant height, which is named as OsSwc4, is derived from rice (Oryzasativvar), encodes OsSWC4 protein, and has an amino acid sequence of SEQ ID No: 2, the nucleotide coding sequence is SEQ ID No: 1. the expression reduction of the protein expressed by the gene can reduce the plant height of rice and reduce the tillering of the rice.

In one aspect, the invention provides a rice protein or polypeptide as set forth in SEQ ID NO: 7 in the rice plant type regulation, the protein is OsSWC4 protein, and the amino acid sequence of the protein is shown as SEQ ID No: 2, respectively.

In one embodiment, the nucleotide coding sequence of the OsSWC4 protein is as set forth in SEQ ID No: 1 is shown.

In one embodiment, wherein the rice plant type is plant height and/or tillering.

In one embodiment, wherein the modulating the plant type of rice is reducing the plant height of rice and/or reducing the number of tillers of rice.

In one embodiment, the variety of rice is nipponica or 9311, preferably nipponica.

In another aspect, the present invention provides a method of modulating plant type in rice, comprising the step of decreasing expression of an OsSWC4 protein, wherein the amino acid sequence of the OsSWC4 protein is SEQ ID No: 2.

In one embodiment, the variety of rice is nipponica or 9311, preferably nipponica.

In one embodiment, wherein the rice plant type is plant height and/or tillering.

In one embodiment, wherein the modulating the plant type of rice is reducing the plant height of rice and/or reducing the number of tillers of rice.

In one embodiment, the step wherein the expression of OsSWC4 protein is reduced is accomplished by gene knock-out, gene knockdown, or the like.

In one embodiment, wherein the gene knockout or gene knockdown method can be any method known in the art, such as a method of RNA interference.

In one embodiment, the method wherein RNA interference is performed using a nucleic acid sequence as set forth in SEQ ID NO: 7 for RNA interference.

Drawings

FIG. 1 shows a schematic representation of OsSwc4 gene RNA interference.

FIG. 2 shows the expression level of OsSwc4 gene in interfering plants.

FIG. 3 shows phenotype observation (FIG. 3a) and plant height and tillering (FIG. 3b) statistics of OsSwc4 RNA interference plants.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to the accompanying drawings in combination with specific embodiments.