阅读说明：本技术 一种杀螨乳油及其制备方法和应用 (Mite-killing missible oil and preparation method and application thereof ) 是由 冯诗喻 林瑞庆 刘丽丹 王新秋 谭志坚 于 2021-06-28 设计创作，主要内容包括：本发明公开了一种杀螨乳油及其制备方法和应用,属于兽药领域。杀螨乳油包括丁香酚1-5份、百里香精油2-4份、甲基丁香酚10-20份和乳化剂1-2份,乳化剂包括丙酮酸钙、白矿油26、乳化剂OP-10和农乳700。本发明的杀螨乳油稀释后药液均匀、稳定、不分层,选用的主要原材料植物精油各活性成分之间有显著协同增效作用,药物安全、绿色、高效,无毒副作用；生产应用上能够有效杀灭鸡体上的新勋恙螨,降低生产成本。(The invention discloses an acaricide missible oil, and a preparation method and application thereof, and belongs to the field of veterinary medicines. The miticidal emulsifiable concentrate comprises 1-5 parts of eugenol, 2-4 parts of thyme essential oil, 10-20 parts of methyl eugenol and 1-2 parts of emulsifier, wherein the emulsifier comprises calcium pyruvate, 26 parts of white mineral oil, OP-10 parts of emulsifier and 700 parts of agricultural emulsion. The acaricidal missible oil disclosed by the invention is uniform and stable in liquid medicine after being diluted, does not layer, has a remarkable synergistic interaction effect among active ingredients of plant essential oil serving as a main raw material, and is safe, green, efficient and free of toxic and side effects; the new chigger on the chicken body can be effectively killed in the production application, and the production cost is reduced.)

1. The acaricidal missible oil is characterized by comprising the following raw materials: eugenol, thyme essential oil, methyl eugenol and emulsifier.

2. An acaricidal emulsifiable concentrate as set forth in claim 1, characterized in that said raw materials are in parts by weight as follows: 1-5 parts of eugenol, 2-4 parts of thyme essential oil, 10-20 parts of methyl eugenol and 1-2 parts of emulsifier.

3. An acaricidal emulsifiable concentrate according to claim 1 or 2, wherein the emulsifier comprises calcium salt, mineral oil, emulsifier OP-10, agricultural emulsion 700.

4. An acaricidal emulsifiable concentrate according to claim 3, wherein the mass ratio of the calcium salt to the mineral oil to the emulsifier OP-10 to the agricultural emulsion 700 is 7: (18-23): (30-35): (18-23).

5. An acaricidal emulsifiable concentrate as set forth in claim 1, characterized in that said raw materials are in parts by weight as follows: 2-3 parts of eugenol, 3-4 parts of thyme essential oil, 14-16 parts of methyl eugenol and 1-1.3 parts of emulsifier.

6. An acaricidal emulsifiable concentrate according to claim 3, characterized in that the calcium salt is selected from calcium pyruvate.

7. An acaricidal cream according to claim 3, characterized in that the mineral oil is chosen from white mineral oil 26.

8. A process for preparing an emulsifiable concentrate as claimed in claim 1, which comprises the steps of:

s1: weighing the components in proportion, heating the solvent, adding the emulsifier into the solvent, and stirring;

s2: adding eugenol, thyme essential oil and methyl eugenol, stirring and mixing to obtain the acaricide missible oil.

9. The method according to claim 8, wherein the heating is performed at a temperature of 40 to 50 ℃ in step S1; in step S2, the stirring speed is 1800-2200 r/min.

10. The use of the emulsifiable concentrate for killing mites of gazang chigger of chickens of claim 1 for the control of chigger mite disease.

Technical Field

The invention belongs to the technical field of veterinary medicines, and particularly relates to an acaricidal missible oil, and a preparation method and application thereof.

Background

Acariasis is a ubiquitous group of ectoparasitic diseases of humans and animals. The disease is characterized by contact infection, severe itching and severe skin inflammation. The occurrence of acariasis is very common and quite persistent, and animals such as rabbits, pigs, sheep, dogs, foxes, mice, cows, chickens and the like and human beings can be infected with acarids. Among them, in the production and sale of Guang chickens, skin disease caused by chigger newly is one of important diseases affecting the product quality. Most of the farms for raising yellow-feathered broilers are located in southern areas of China, the warm and humid climate is more suitable for breeding chiggers newly, and the grazing stadium located on the bamboo forest and shrub sides is a hotbed for breeding chiggers newly. Therefore, many breeding enterprises actively deploy the prevention and control work of new chiggers of broiler chickens, and strive to ensure the quality of Guangji products.

For a long time, the control of animal acariasis relies on acaricidal drugs such as avermectins, pyrethrins, amitraz, dicofol, trichlorfon and the like, and with the wide, long-term and frequent application of these drugs, more and more acarids have developed increasingly serious drug resistance, and the recurrence and repeated infection of acariasis become frequent. And the mite killing efficiency of the mite killing medicine aiming at preventing and treating the chigger disease of the broilers is low, and the insect expelling rate can be improved and the mite disease recurrence can be prevented only by repeatedly using the medicine for many times. Therefore, research and development of a green and safe acaricidal medicament with higher acaricidal efficiency are needed.

Disclosure of Invention

The present invention is directed to solving at least one of the problems of the prior art described above. Therefore, the invention provides an acaricide missible oil, and a preparation method and application thereof.

According to one aspect of the invention, the acaricidal missible oil comprises the following raw materials: eugenol, thyme essential oil, methyl eugenol and emulsifier.

In some embodiments of the invention, the starting materials are in the following parts by weight: 1-5 parts of eugenol, 2-4 parts of thyme essential oil, 10-20 parts of methyl eugenol and 1-2 parts of emulsifier.

In some embodiments of the invention, the emulsifier comprises calcium salt, mineral oil, emulsifier OP-10, agricultural milk 700.

In some embodiments of the invention, the mass ratio of the calcium salt, mineral oil, emulsifier OP-10 and farm milk 700 is 7: (18-23): (30-35): (18-23).

In some embodiments of the invention, the starting materials are in the following parts by weight: 2-3 parts of eugenol, 3-4 parts of thyme essential oil, 14-16 parts of methyl eugenol and 1-1.3 parts of emulsifier.

In some embodiments of the invention, the calcium salt is selected from calcium pyruvate.

In some embodiments of the invention, the mineral oil is selected from white mineral oil 26.

The invention also provides a preparation method of the acaricidal missible oil, which comprises the following steps:

s1: weighing the components in proportion, heating the solvent, adding the emulsifier into the solvent, and stirring;

s2: adding eugenol, thyme essential oil and methyl eugenol, stirring and mixing to obtain the acaricide missible oil.

In some embodiments of the invention, in step S1, the solvent is ethyl acetate.

In some embodiments of the invention, the heating temperature is 40-50 ℃ in step S1.

In some embodiments of the present invention, in step S2, the stirring speed is 1800-2200 r/min.

The invention also provides application of the mite-killing missible oil in prevention and treatment of chigger disease of chicken.

According to a preferred embodiment of the present invention, at least the following advantages are provided:

the acaricidal missible oil disclosed by the invention is uniform and stable in liquid medicine after being diluted, does not layer, has a remarkable synergistic interaction effect among active ingredients of plant essential oil serving as a main raw material, and is safe, green, efficient and free of toxic and side effects; the new chigger on the chicken body can be effectively killed in the production application, and the production cost is reduced.

Detailed Description

The concept and technical effects of the present invention will be clearly and completely described below in conjunction with the embodiments to fully understand the objects, features and effects of the present invention. It is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and those skilled in the art can obtain other embodiments without inventive effort based on the embodiments of the present invention, and all embodiments are within the protection scope of the present invention.

Example 1

The acaricidal missible oil is composed of the following raw materials in parts by weight: 2 parts of eugenol, 3 parts of thyme essential oil, 15 parts of methyl eugenol and 1 part of emulsifier, wherein the emulsifier consists of calcium pyruvate, 26 parts of white mineral oil, OP-10 parts of emulsifier and 700 parts of agricultural emulsion according to the mass ratio of 7:20:33:20, and the obtained acaricidal missible oil is light milky transparent clear liquid, has uniform color and stable property.

The preparation method of the acaricide missible oil comprises the following steps: heating ethyl acetate to 45 ℃, adding the emulsifier components into the ethyl acetate, and stirring for dissolving; adding eugenol, thyme essential oil and methyl eugenol, stirring and mixing at 2000r/min to obtain the miticide emulsifiable concentrate.

Example 2

The acaricidal missible oil is composed of the following raw materials in parts by weight: 1.5 parts of eugenol, 3 parts of thyme essential oil, 15 parts of methyl eugenol and 1 part of emulsifier, wherein the emulsifier consists of calcium pyruvate, 26 parts of white mineral oil, OP-10 parts of emulsifier and 700 parts of agricultural emulsion according to the mass ratio of 7:20:33:20, and the obtained acaricidal missible oil is light milky transparent clear liquid, has uniform color and stable property.

The preparation method of the acaricide missible oil comprises the following steps: heating ethyl acetate to 45 ℃, adding the emulsifier components into the ethyl acetate, and stirring for dissolving; adding eugenol, thyme essential oil and methyl eugenol, stirring and mixing at 2000r/min to obtain the miticide emulsifiable concentrate.

Example 3

The acaricidal missible oil is composed of the following raw materials in parts by weight: 2 parts of eugenol, 2 parts of thyme essential oil, 10 parts of methyl eugenol and 1 part of emulsifier, wherein the emulsifier consists of calcium pyruvate, 26 parts of white mineral oil, OP-10 parts of emulsifier and 700 parts of agricultural emulsion according to the mass ratio of 7:20:33:20, and the obtained acaricidal missible oil is light milky transparent clear liquid, has uniform color and stable property.

The preparation method of the acaricide missible oil comprises the following steps: heating ethyl acetate to 50 ℃, adding the emulsifier components into the ethyl acetate, and stirring for dissolving; adding eugenol, thyme essential oil and methyl eugenol, stirring and mixing at 1800r/min to obtain the miticide emulsifiable concentrate.

Example 4

The acaricidal missible oil is composed of the following raw materials in parts by weight: 2 parts of eugenol, 4 parts of thyme essential oil, 15 parts of methyl eugenol and 1 part of emulsifier, wherein the emulsifier consists of calcium pyruvate, 26 parts of white mineral oil, OP-10 parts of emulsifier and 700 parts of agricultural emulsion according to the mass ratio of 7:20:33:20, and the obtained acaricidal missible oil is light milky transparent clear liquid, has uniform color and stable property.

The preparation method of the acaricide missible oil comprises the following steps: heating ethyl acetate to 40 ℃, adding the emulsifier components into the ethyl acetate, and stirring for dissolving; adding eugenol, thyme essential oil and methyl eugenol, stirring and mixing at 2200r/min to obtain the miticide emulsifiable concentrate.

Comparative example 1

The acaricidal missible oil is different from the acaricidal missible oil in example 1 in that thyme essential oil is not added, and the acaricidal missible oil specifically comprises the following components: 5 parts of eugenol, 15 parts of methyl eugenol and 1 part of emulsifier, wherein the emulsifier consists of calcium pyruvate, 26 parts of white mineral oil, OP-10 parts of emulsifier and 700 parts of agricultural emulsion according to the mass ratio of 7:20:33:20, and the obtained acaricidal missible oil is colorless clear liquid, has uniform color and luster and stable property.

The preparation method of the acaricide missible oil comprises the following steps: heating ethyl acetate to 45 ℃, adding the emulsifier components into the ethyl acetate, and stirring for dissolving; adding eugenol and methyl eugenol, stirring and mixing at 2000r/min to obtain the miticide emulsifiable concentrate.

Comparative example 2

The acaricidal missible oil is different from the acaricidal missible oil in example 1 in that eugenol and thyme essential oil are not added, and the acaricidal missible oil specifically comprises the following components: 20 parts of methyl eugenol and 1 part of emulsifier, wherein the emulsifier consists of calcium pyruvate, white mineral oil 26, emulsifier OP-10 and agricultural emulsion 700 according to the mass ratio of 7:20:33:20, and the obtained acaricidal missible oil is colorless clear liquid, and has uniform color and stable property.

The preparation method of the acaricide missible oil comprises the following steps: heating ethyl acetate to 45 ℃, adding the emulsifier components into the ethyl acetate, and stirring for dissolving; then adding methyl eugenol, stirring and mixing at 2000r/min to prepare the acaricide missible oil.

The first test example: acaricidal effect test of acaricidal missible oil

Scheme 1: 5 chiggers each group were collected from the affected chickens, and the miticidal emulsifiable concentrates of examples 1-4 and comparative examples 1-2 were mixed as follows: and (5) diluting by 2000 times. The death time of each group of new gazania was as shown in table 1.

TABLE 1

As can be seen from Table 1, the mite-killing emulsifiable concentrate for preventing and controlling New tsutsugamushi provided in example 1 of the present invention was prepared in the following manner: the 2000-fold dilution can kill new chigger within 5 minutes, but the killing time of comparative example 1 without adding thyme essential oil and comparative example 2 only using methyl eugenol is longer, which shows that the compound use of eugenol, thyme essential oil and methyl eugenol in the invention has obvious synergistic effect.

Scheme 2: the miticidal emulsifiable concentrate is delivered to different farmers of south-Ningwenshi of Guangxi province for animal tests, and is applied according to effective concentration, and test results are fed back as follows.

1, family maintenance: the chicken of the Shandong Dynasty of 64 days old. The house is a long-term mite house, the mites are serious before the drug is taken, most of the mites scab after the mites are observed on the 7 th day after the mites are directly sprayed on chicken bodies, the cure rate is over 98 percent, and the chickens only have no skin red swelling, injury and inflammation.

And (3) family maintenance: the chicken of the Shandong Dynasty of 66 days old. The house is a long-term mite house, the mites are not serious before the drug is taken, only the mites exist individually, the drug bath is used, the padding for raising the house is better managed, the treatment effect is good, the cure rate is more than 98%, and the chickens only have no skin swelling, injury and inflammation.

Test example two: stability test of miticidal emulsifiable concentrates

The test was carried out by referring to accelerated test measurement in stability of the first edition of pharmacopoeia of the people's republic of China 2015. Examples 1 and 2 were stored at 40 ℃. + -. 2 ℃ and 70%. + -. 5% relative temperature for 6 months, the temperature and humidity were monitored in real time, and samples were taken at the end of 1 month, 2 months, 3 months and 6 months during the test period to measure the content of plant essential oil.

The test results are shown in the table 2.

TABLE 2 stability test results of miticidal emulsifiable concentrates

The results in Table 2 show that the acaricide missible oil has the advantages of less content change, stable property, uniform liquid medicine and no layering after accelerated tests.

The embodiments of the present invention have been described in detail, but the present invention is not limited to the embodiments, and various changes can be made without departing from the gist of the present invention within the knowledge of those skilled in the art. Furthermore, the embodiments of the present invention and the features of the embodiments may be combined with each other without conflict.