阅读说明：本技术 石笔木提取物在制备α-葡萄糖苷酶抑制剂组合物中的应用 (Application of Shiwo kaki extract in preparation of alpha-glucosidase inhibitor composition ) 是由 史丽颖 王柳 于大永 于 2020-07-21 设计创作，主要内容包括：本发明涉及石笔木提取物在制备α-葡萄糖苷酶抑制剂组合物中的应用,属于医药技术领域。主要技术方案为：石笔木提取物在制备α-葡萄糖苷酶抑制剂组合物中的应用,所述的石笔木提取物为选自石笔木花、果皮、种子的一种或一种以上的提取物。本发明提供了一种石笔木不同部位的提取物在制备α-葡萄糖苷酶抑制剂组合物的应用,石笔木提取物对α-葡萄糖苷酶抑制活性强于阿卡波糖,可制成各种固体或液体口服制剂,或者加入其它成份和辅料制成复方药物或保健品用于治疗和预防糖尿病。(The invention relates to an application of a shiitake extract in preparation of an alpha-glucosidase inhibitor composition, belonging to the technical field of medicines. The main technical scheme is as follows: the application of the crassula argentea extract in preparing the alpha-glucosidase inhibitor composition is characterized in that the crassula argentea extract is one or more than one extract selected from crassula argentea flower, pericarp and seed. The invention provides application of extracts of different parts of crassula argentea in preparing alpha-glucosidase inhibitor composition, wherein the crassula argentea extract has stronger alpha-glucosidase inhibition activity than acarbose, and can be prepared into various solid or liquid oral preparations, or can be added with other components and auxiliary materials to prepare compound medicines or health products for treating and preventing diabetes.)

1. The application of the crassula argentea extract in preparing the alpha-glucosidase inhibitor composition is characterized in that the crassula argentea extract is one or more than one extract selected from crassula argentea flower, pericarp and seeds.

2. The use of the Shiwo kaki extract as claimed in claim 2 in the preparation of alpha-glucosidase inhibitor drugs, wherein the Shiwo kaki extract is prepared by reflux extraction with ethanol or water as an extractant and concentration.

3. The use of the Shiwo kaki extract in the preparation of an alpha-glucosidase inhibitor composition as claimed in claim 2, wherein the ethanol extract or water extract of the Shiwo kaki flowers, fruit peels, seeds is obtained by heating and refluxing with 95% ethanol or water at a ratio of 1g to 3ml for 2 times, each time for 60min, combining the extracts, concentrating with a rotary evaporator and recovering ethanol or water.

4. The use of an extract of Shiwo sieboldii as claimed in claim 1 for the preparation of an α -glucosidase inhibitor composition, wherein said α -glucosidase inhibitor composition is a medicament for the treatment and prevention of diabetes.

5. Use of an extract of shiitake tree as claimed in claim 4 for the preparation of an α -glucosidase inhibitor composition, wherein the α -glucosidase inhibitor composition is a solid or liquid oral formulation.

6. The use of the Shiwo sieboldii extract in the preparation of an alpha-glucosidase inhibitor composition as claimed in claim 4, wherein the alpha-glucosidase inhibitor composition is a health product for the treatment and prevention of diabetes.

Technical Field

The invention relates to the technical field of medicines, in particular to application of extracts of different parts of crassula argentea to preparation of an alpha-glucosidase inhibitor.

Background

At present, the prevalence rate of diabetes mellitus of adults in China is about 11 percent, and the number of diabetics is estimated to be 1.14 hundred million. Although various hypoglycemic drugs with different action mechanisms exist at present, 63 percent of patients with type II diabetes cannot be effectively treated. Hyperglycemia is a major feature of diabetes and is also a significant cause of diabetic complications. The incidence of diabetes rises year by year, and great influence is brought to the health and daily life of people.

The alpha-glucosidase inhibitor can competitively inhibit various alpha-glucosidase in small intestine and slow down the decomposition speed of starch into glucose, thereby slowing down the absorption of glucose in intestinal tract and reducing postprandial blood sugar. At present, the traditional Chinese medicine composition is widely applied to prevention and treatment of diabetes and complications thereof. The alpha-glucosidase inhibitor hypoglycemic agents which are on the market and applied clinically at present mainly comprise: acarbose, voglibose, miglitol. The natural plant resources in China are rich, the cost is low, the alpha-glucosidase inhibitor is one of the main sources of the natural alpha-glucosidase inhibitor, and the alpha-glucosidase inhibitor has certain development value.

The young leaves of the young buds of the callicarpa steleocarpa are often drunk as tea, have the effects of refreshing and restoring consciousness, have high medicinal value, and can be used for treating cold, enteritis, dysentery or diarrhea caused by eating greasy food.

Disclosure of Invention

In order to make up the defects of the prior art, the invention provides the application of the shiitake extract in preparing the alpha-glucosidase inhibitor composition. The technical scheme of the invention is as follows: the application of the crassula argentea extract in preparing the alpha-glucosidase inhibitor composition is characterized in that the crassula argentea extract is one or more than one extract selected from crassula argentea flower, pericarp and seed.

Further, the crabapple extract is prepared by performing reflux extraction and concentration on the crabapple extract by using ethanol or water as an extractant.

Further, the extract is obtained by extracting according to the following method: pulverizing collected maidenhair flower, pericarp and seed, extracting with 95% ethanol or water under reflux at a ratio of 1g to 3ml for 2 times (60 min each time), mixing extractive solutions, concentrating with rotary evaporator, and recovering ethanol or water to obtain ethanol extract or water extract of maidenhair flower, pericarp and seed.

Furthermore, the alpha-glucosidase inhibitor composition is a medicament for treating and preventing diabetes.

Further, the alpha-glucosidase inhibitor composition is a solid or liquid oral preparation.

Furthermore, the alpha-glucosidase inhibitor composition is a health-care product for treating and preventing diabetes.

The extract is subjected to alpha-glucosidase activity measurement, and the measurement result shows that the ethanol extract and the water extract of the common stonecrop herb, the pericarp and the seed can inhibit the alpha-glucosidase activity, and the half Inhibition Concentration (IC) of the ethanol extract and the water extract can inhibit the alpha-glucosidase activity₅₀) 2.49-40.77 mu g/mL, which are all stronger than the activity (IC) of acarbose₅₀＝484.9μg/mL)。

The invention has the following beneficial effects: the invention provides application of extracts of different parts of crassula argentea in preparing alpha-glucosidase inhibitor composition, wherein the crassula argentea extract has stronger alpha-glucosidase inhibition activity than acarbose, and can be prepared into various solid or liquid oral preparations, or can be added with other components and auxiliary materials to prepare compound medicines or health products for treating and preventing diabetes.

Detailed Description

The present invention will be further illustrated and explained with reference to specific examples, but the present invention is not limited to these specific examples. The methods used in the examples are conventional technical methods in the field unless otherwise specified; the materials, reagents and the like used in the kit are commercially available, unless otherwise specified. Preferably, the maitake flowers and fruits are collected from Guangdong uncovered positive white tower town in 2019 and 6 months, and are identified as large-stone-fruit-tea Pyrenaria spectabilis (Champ.) C.Y.Wu et S.X.Yang ex S.X.Yang by professor of college Cao Hui of Jinnan university, Tutcheria austria-sinica Chang of the genus Camellia of the family Theaceae, and the specimens are currently stored in the college of Life sciences and technology of Daizian university (specimen numbers 2019062001, 2019062002, 2019062003). Alpha-glucosidase was purchased from Sigma; 4-Nitrophenyl- α -D-glucopyranoside (4-nitrophenyl- α -D-glucopyranoside, PNPG) was purchased from Bailingwei science and technology, Inc.; acarbose (acarbose) was purchased from Shanghai-derived leaf Biotech, Inc. The other reagents are analytically pure.