阅读说明：本技术 一种井场含油污泥微生物深度处理方法 (Well site oily sludge microorganism deep treatment method ) 是由 王光义 张道法 张进科 李博良 翟文 艾翔 张继文 赵鹏 于 2021-08-24 设计创作，主要内容包括：本发明属于污泥处理技术领域,具体涉及一种井场含油污泥微生物深度处理方法,包括以下步骤：制备酵母菌菌液、芽孢杆菌菌液、石油降解菌菌液,上述各菌液混合后的得到混合菌液；将井场含油污泥转运至存放池中,然后向存放池中放入微生物输入架；将混合菌液加入到微生物输入架中,则菌液流至各个所述通道管中,静置20-40min后,通过注入高压气体,使高压气体将可降解负载膜冲破,并使所述通道管内的物料混到含油污泥中；自然发酵,直至有机物含量达到排放要求,完成污泥处理。本发明设置了存放池、微生物输入架和微生物菌液,既减小了污泥处理过程中的搅拌难度,又开发了新的降解石油烃的污泥处理方法。(The invention belongs to the technical field of sludge treatment, and particularly relates to a well site oily sludge microorganism deep treatment method, which comprises the following steps: preparing a saccharomycete liquid, a bacillus liquid and a petroleum degrading bacterium liquid, and mixing the saccharomycete liquid, the bacillus liquid and the petroleum degrading bacterium liquid to obtain a mixed bacterium liquid; transferring oily sludge in a well site to a storage pool, and then putting a microorganism input rack into the storage pool; adding the mixed bacterial liquid into a microorganism input frame, allowing the bacterial liquid to flow into each channel pipe, standing for 20-40min, injecting high-pressure gas to break the degradable load membrane by the high-pressure gas, and mixing the materials in the channel pipes into the oily sludge; naturally fermenting until the content of organic matters meets the discharge requirement, and finishing sludge treatment. The invention is provided with the storage pool, the microorganism input frame and the microorganism liquid, thereby not only reducing the stirring difficulty in the sludge treatment process, but also developing a new sludge treatment method for degrading petroleum hydrocarbon.)

1. A method for deeply treating oily sludge microorganisms at a well site is characterized by comprising the following steps:

s1, preparing a yeast liquid, a bacillus liquid and a petroleum degrading bacteria liquid, and mixing the above bacteria liquids to obtain a mixed bacteria liquid for later use;

an anti-seepage and anti-leakage storage pool (1) is made in a well site, oily sludge in the well site is transferred into the storage pool (1), and then a microorganism input frame is placed into the storage pool (1);

the microorganism input frame is formed by cross connection of a plurality of channel pipes (2), the channel pipes (2) are communicated, the pipe wall of each channel pipe (2) is of a hollowed-out net structure and is provided with a plurality of liquid outlets, one wide port of one channel pipe (2) is open, the other end of each channel pipe (2) and pipe orifices at two ends of the other channel pipes (2) are both blind ends, a degradable load membrane (3) is laid on the periphery of each channel pipe (2), the degradable load membrane (3) seals all the liquid outlets of the corresponding channel pipe (2), and biochar is loaded between the channel pipe (2) and the degradable load membrane (3) on the inner wall of the degradable load membrane (3);

when the microorganism input frame is put into the storage pool (1), the open end of the channel pipe (2) is positioned outside the upper surface of the oily sludge;

s2, adding the mixed bacteria liquid into the microorganism input frame from the open end of the channel pipe, standing for 20-40min, then injecting high-pressure gas to break the degradable load membrane (3), and mixing the materials in the channel pipe (2) into the oily sludge;

and S3, naturally fermenting until the petroleum content in the oily sludge meets the discharge requirement, and finishing sludge treatment.

2. The deep treatment method of oily sludge microorganisms at well site as claimed in claim 1, wherein the channel pipe (2) comprises a longitudinal pipe and a plurality of transverse pipes fixedly connected to the longitudinal pipe, each transverse pipe is communicated with the longitudinal pipe, the pipe walls of the transverse pipes and the longitudinal pipe are both of a net structure, and the bottom of the longitudinal pipe is provided with a base; the upper end of the longitudinal pipeline is an open end, and the lower end of the longitudinal pipeline and the two ends of all the transverse pipelines are blind ends.

3. The advanced treatment method for oily sludge microorganisms at well site according to claim 1 or 2, characterized in that the degradable load membrane (3) is a degradable starch film or a degradable mulching film.

4. The method of claim 3, wherein the biochar is 40-100 mesh biochar powder.

5. The advanced treatment method for oily sludge microorganisms at well sites as claimed in claim 1, wherein the yeast is Saccharomyces cerevisiae CGMCC NO.12417, the bacillus is Bacillus megaterium CGMCC NO.3770, and the petroleum degrading bacteria are Corynebacterium glutamicum CGMCC NO.8647, BDB-n biodegradation bacteria or BDB-a biodegradation bacteria.

6. The advanced treatment method for microorganisms in oily sludge at well site according to claim 5, wherein the viable count of the yeast bacterial liquid, the bacillus bacterial liquid and the petroleum degrading bacterial liquid is 10⁷More than one/mL, and the mass ratio of the saccharomycete liquid to the bacillus liquid to the petroleum degrading bacteria liquid in the mixed bacteria liquid is 1:1: 2-3.

7. The deep treatment method of microorganisms in oily sludge at well site as recited in claim 6, wherein the addition amount of the mixed bacterial liquid is 5-10L per cubic meter of oily sludge.

8. The method of claim 7, wherein the high pressure gas is air.

9. The advanced treatment method for microorganisms in oily sludge at well sites as claimed in claim 7, wherein the mixed bacteria liquid is added to the microorganism input rack in batches in S2, and the operation is circulated according to the modes of adding the mixed bacteria liquid, standing for 20-40min and injecting high-pressure gas until all the mixed bacteria liquid is added.

10. The advanced treatment method for oily sludge microorganisms at well sites as claimed in claim 9, wherein the fermentation time is 60-90 days.

Technical Field

The invention belongs to the technical field of sludge treatment, and particularly relates to a microbial advanced treatment method for oily sludge in a well site.

Background

The oily sludge of the well site is inevitably formed in the field construction and daily production operation processes of the oil field, and contains a large amount of organic matters such as petroleum hydrocarbon and chemical agent residues, which affect the ecological environment of the mining area of the oil field, so the petroleum hydrocarbon and chemical agent residues in the oily sludge of the well site must be deeply treated to protect the ecological environment of the mining area.

In the prior art, the treatment method of the oily sludge mainly comprises the following steps:

(1) the separation method is characterized in that the sludge is treated by a plurality of steps, oil, water, soil particles and the like are separated, and the oil-removed soil is treated in a landfill mode or a building material preparation mode. For example, Yangxiaogang, Yangxiagang, Weiyanglin, Yangxiang oil field oil-containing sludge treatment field test research [ J ] oil and natural gas chemical industry, 2013,42(006): 654: -657.) about the treatment of sludge in an extended oil field, the sludge is diluted by a hot washing device, three-phase separation of oil, mud and water is carried out by a three-phase separator, the separated oil enters an oil storage tank to be recovered, the separated water enters a water treatment device to be recycled to the hot washing step, and the separated mud enters a filter pressing device to be compressed for landfill or manufacturing of building mineral aggregate. The method and the matching device designed by Yangyinggang et al not only separate the oil from the oily sludge, but also recycle the oil, water and sludge respectively, thereby protecting the environment and achieving the maximum resource utilization.

(2) Chemical method, the method is to utilize some chemical reagents to treat the sludge to degrade the organic matters in it, such as the widmanyanglin, etc. (widmanyanglin, yangmjust, lulei, etc.. experiments in oily sludge treatment room [ J ]. oil and gas field ground engineering, 2015(07):17-19.) based on bio-enzyme compound agent, it utilizes bio-enzyme compound emulsifier containing bio-enzyme, surfactant, carbon tetrachloride and petroleum ether to treat the sludge, it takes protein as inactive catalyst, accelerates the reaction speed, and improves the sludge treatment rate and oil recovery rate.

(3) The microbiological method, the above (1) to (2) are suitable for the situation that the oil content in the sludge is high, in order to avoid the waste of oil, the oil is recycled, but some sludge with low oil content appears in the oil exploitation process, if a complex oil, sludge and water separation recycling technology is adopted, a large amount of equipment resources are consumed, but the separated oil amount is very low, so the sludge with low oil content is generally treated by microbiological degradation. The method comprises the steps of selecting microorganisms with the function of degrading organic matters, adding culture solution of the microorganisms into sludge, reducing the content of the organic matters in the sludge after long-time treatment, and sterilizing the treated sludge in modes of insolation and the like, so that the treated sludge can be used as a matrix for increasing the soil fertility.

In any treatment mode, the content of oil in the mud is reduced as a standard, but the separation method has too many treatment steps and high treatment cost; chemical processes consume large amounts of compounds and, while reducing the content of organic matter in the sludge, may cause contamination with other compounds. The microbial method is simple in operation and not easy to cause new pollution, so that the method becomes a better sludge treatment mode, but a plurality of microbial functions are degraded, and when the microbial method is used for a plurality of times, the degradation capability of organic matters is reduced, so that a new microbial sludge treatment method needs to be continuously developed.

Disclosure of Invention

In order to solve the technical problems, the invention provides a method for deeply treating oily sludge in a well site by using microorganisms, which is a novel technology for deeply treating the oily sludge by using the microorganisms.

The invention aims to provide a microbial advanced treatment method for oily sludge in a well site, which comprises the following steps:

s1, preparing a yeast liquid, a bacillus liquid and a petroleum degrading bacteria liquid, and mixing the above bacteria liquids to obtain a mixed bacteria liquid for later use;

an anti-seepage and anti-leakage storage pool is made in a well site, oily sludge in the well site is transferred into the storage pool, and then a microorganism input frame is placed into the storage pool;

the structure of the microorganism input frame is as follows: the microorganism input frame is formed by cross-connecting a plurality of channel pipes, the channel pipes are communicated, the pipe walls of the channel pipes are of a hollowed-out net structure and are provided with a plurality of liquid outlets, one wide port of one channel pipe is open, the other end of the channel pipe and pipe orifices at two ends of the other channel pipes are blind ends, a degradable load membrane is laid on the periphery of each channel pipe and closes all the liquid outlets of the corresponding channel pipe, and biochar is loaded on the inner wall of the degradable load membrane and the position between the channel pipe and the degradable load membrane;

when the microorganism input frame is placed into the storage pool, the open end of the channel pipe is positioned outside the upper surface of the oily sludge;

s2, adding the mixed bacteria liquid into the microorganism input frame from the open end of the channel pipe, allowing the bacteria liquid to flow into each channel pipe, standing for 20-40min, allowing most of bacteria in the mixed bacteria liquid to be adsorbed on charcoal, injecting high-pressure gas to break the degradable load membrane, and mixing the materials in the channel pipe into oily sludge;

and S3, performing natural fermentation, and periodically sampling and detecting from the oily sludge until the petroleum content in the oily sludge meets the discharge requirement, thereby completing sludge treatment.

Preferably, in the above method for advanced treatment of oily sludge at well site, the channel pipe comprises a longitudinal pipe and a plurality of transverse pipes fixedly connected to the longitudinal pipe, each of the transverse pipes is communicated with the longitudinal pipe, the pipe walls of the transverse pipes and the longitudinal pipe are both of a net structure, and the bottom of the longitudinal pipe is provided with a base; the upper end of the longitudinal pipeline is an open end, and the lower end of the longitudinal pipeline and the two ends of all the transverse pipelines are blind ends.

Preferably, in the method for deeply treating the oily sludge in the well site by using the microorganisms, the degradable load membrane is a degradable starch film or a degradable mulching film.

Preferably, in the method for the deep treatment of the oily sludge microorganisms at the well site, the biochar is 40-100 meshes of biochar powder, and the loading thickness of the biochar powder is 0.5-1 cm.

Preferably, in the advanced treatment method for the oily sludge in the well site, the yeast is saccharomyces cerevisiae CGMCC NO.12417, the bacillus is bacillus megaterium CGMCC NO.3770, and the petroleum degrading bacteria are corynebacterium glutamicum CGMCC NO.8647, BDB-n biodegradable bacteria or BDB-a biodegradable bacteria.

Preferably, in the advanced treatment method for the oily sludge microorganisms in the well site, the viable count of the yeast bacterial liquid, the bacillus bacterial liquid and the petroleum degrading bacterial liquid is 10⁷The number per mL is of order or more, and the mass ratio of the saccharomycete liquid to the bacillus liquid to the petroleum degrading bacteria liquid in the mixed bacteria liquid is 1:1: 2-3.

Preferably, the well site comprisesThe deep treatment method of the oily sludge microorganisms comprises the steps that 5-10L of mixed bacterial liquid is added to each cubic meter of oily sludge, and the storage pool contains 20-100m of oily sludge³. If the amount of the oily sludge is large, a plurality of storage pools can work simultaneously.

Preferably, in the method for advanced treatment of oily sludge microorganisms at well sites, the high-pressure gas is air. When high-pressure gas is introduced, the sludge treatment agent has a mixing effect on oil-containing sludge, so that microorganisms are fully dispersed into the oil-containing sludge.

Preferably, in the advanced treatment method for the oily sludge in the well site, the mixed bacterial liquid is added to the microorganism input rack in S2 in batches, and the operation is circulated according to the modes of adding the mixed bacterial liquid, standing for 20-40min and injecting high-pressure gas until all the mixed bacterial liquid is added.

Preferably, the fermentation time of the deep treatment method of oily sludge microorganisms at the well site is 60-90 days.

Compared with the prior art, the invention has the following beneficial effects:

1. the invention is provided with the storage tank and the microorganism input frame with special structures, and the biochar, the microorganism liquid and the sludge are mixed by adopting a high-pressure gas injection mode, so that the function of a common stirring device is replaced, and the stirring difficulty is reduced by the structure of the invention because the sludge has higher viscosity and is difficult to stir.

2. The invention utilizes the adsorption effect of the biochar to adsorb thalli on the surface of the biochar, a large number of micro-fermentation centers are formed after the biochar-loaded thalli are mixed into sludge, and the biochar can also adsorb oil in the sludge on the surface of the biochar for microbial degradation, so that the degradation efficiency can be improved. The oil content in the treated sludge is reduced, and after the sludge is sterilized, the biochar can be used as organic matter nutrition, and the microbial thallus can be used as a compound nutrition source such as a nitrogen source, a trace element source and the like, so that the sludge can be used as a base fertilizer or a soil improvement matrix.

3. Because the biochar is powder which is not easy to dissolve in water, if the biochar is directly mixed with bacterial liquid and then added into sludge, powder agglomeration is easy to cause, and the dispersibility of the biochar is poor.

4. Experiments prove that the saccharomyces cerevisiae CGMCC NO.12417, the bacillus megaterium CGMCC NO.3770, the corynebacterium glutamicum CGMCC NO.8647, the BDB-n biodegradable bacteria and the BDB-a biodegradable bacteria have good petroleum degradation effect.

Drawings

FIG. 1 is a schematic view showing a structure of a microorganism input rack and a storage tank according to the present invention;

fig. 2 is a schematic longitudinal sectional structure of the passage tube of the present invention.

Detailed Description

In order that those skilled in the art will better understand the technical solutions of the present invention to be implemented, the present invention will be further described with reference to the following specific embodiments and accompanying drawings.

In the description of the present invention, reagents used are commercially available and methods used are conventional in the art, unless otherwise specified. The saccharomyces cerevisiae CGMCC NO.12417, the bacillus megatherium CGMCC NO.3770 and the corynebacterium glutamicum CGMCC NO.8647 are purchased from China general microbiological culture Collection center (CGMCC); the BDB-n biodegradable bacteria and the BDB-a biodegradable bacteria are sold in the market and are products developed by the Nanyang Donghua company and the Beijing university environmental college; the above-mentioned method for the expanded culture of each bacterial suspension was carried out by a conventional method, or commercially available bacterial suspensions were directly purchased. The oily sludge used in the invention is from an oil field well site of prolonged petroleum, the mass fraction of the oil in the sludge is 7.33 +/-0.21 g/100g, the mass fraction of the sludge is 59.8 +/-1.41 g/100g, and the balance is a mixture of water, metal elements and the like, and the heavy metal content in the sludge-containing sample used in the invention meets the agricultural sludge pollutant control standard GB 4284-2018.

It should be noted that, in the present invention, all the test data are randomly sampled and measured three times respectively, and are expressed in the form of "mean ± standard deviation".

It should be noted that, in the invention, the pressure of the high-pressure air cannot be too large, so as to prevent the sludge from splashing, and the air pressure capable of breaking the degradable load membrane 3 can be tested well in advance; or a plurality of flow testers are arranged in the longitudinal pipeline or the transverse pipeline, when the degradable load membrane 3 is broken, if high-pressure air is stopped being injected, sludge enters the pipeline, and the flow change condition is tested by the flow testers.

It should be noted that, in the embodiments and experimental examples of the present invention, the inner bottom and the periphery of the storage tank 1 are subjected to anti-seepage and anti-leakage treatment, and the storage tank 1, the channel pipe 2 and the degradable load membrane 3 do not contain petroleum.

The invention provides a method for deeply treating oily sludge microorganisms at a well site, which comprises the following embodiments.

Example 1

A method for deeply treating oily sludge microorganisms at a well site comprises the following steps:

s1, preparing a yeast liquid, a bacillus liquid and a petroleum degrading bacteria liquid, and mixing the above bacteria liquids to obtain a mixed bacteria liquid for later use; the yeast is Saccharomyces cerevisiae CGMCC NO.12417, and the concentration of the bacterial liquid is 2 x 10⁷Each mL, the bacillus is bacillus megaterium CGMCC NO.3770, and the concentration of the bacterial liquid is 8 multiplied by 10⁷each/mL, the petroleum degrading bacteria is Corynebacterium glutamicum CGMCC NO.8647, and the concentration of the bacteria liquid is 7 multiplied by 10⁷Per mL; the mass ratio of the saccharomycete liquid to the bacillus liquid to the petroleum degrading bacteria liquid is 1:1: 2;

will be 20m³The well site oily sludge is transferred into a storage pool 1, the depth of the storage pool 1 is 2m, the length of the storage pool is 4m, and the width of the storage pool is 4m, and then a microorganism input frame is placed into the storage pool 1;

the structure of the microorganism input frame is as follows: the microorganism input frame is formed by cross-connecting a plurality of channel tubes 2; the channel pipe 2 comprises a longitudinal pipe and a plurality of transverse pipes fixedly connected to the longitudinal pipe, a layer of transverse pipe is arranged at the height of 20cm, 4 transverse pipes are uniformly distributed around the longitudinal pipe at each layer, the length of each transverse pipe is 1.8m, each transverse pipe is communicated with the longitudinal pipe, the pipe walls of the transverse pipes and the longitudinal pipe are of net structures and are provided with a plurality of liquid outlets, and a base is arranged at the bottom of the longitudinal pipe; the upper end of the longitudinal pipeline is an open end, and the lower end of the longitudinal pipeline and the two ends of all the transverse pipelines are blind ends; the longitudinal pipeline and the transverse pipeline are both round pipes, and the inner diameter of each pipe is 3 cm;

a degradable loading membrane 3 is laid outside each of the longitudinal pipeline and the transverse pipeline, the degradable loading membrane 3 is a degradable starch film, all liquid outlets of the corresponding channel pipe 2 are closed by the degradable loading membrane 3, the inner wall of the degradable loading membrane 3 and the position between the channel pipe 2 and the degradable loading membrane 3 are loaded with 40-mesh charcoal powder, the charcoal powder can be loaded on the degradable loading membrane 3 in an adhesion mode, and the loading thickness of the charcoal powder is 0.5 cm; when the microorganism input frame is put into the storage pool 1, the open end of the channel pipe 2 is positioned outside the upper surface of the oily sludge;

s2, adding 100L of mixed bacteria liquid into the microorganism input frame from the open end of the channel pipe, allowing the bacteria liquid to flow into each channel pipe 2, standing for 20min, allowing most of bacteria in the mixed bacteria liquid to be adsorbed on charcoal, injecting high-pressure air to break the degradable load membrane 3, and allowing the materials in the channel pipe 2 to be mixed into oily sludge;

and S3, naturally fermenting for 60 days, mixing the sludge after uniform treatment, sampling and testing the oil content in the sludge to be 1.17 +/-0.19 g/100g, and the oil removal rate is ((7.33-1.17)/7.33) multiplied by 100 percent to be 84.04 percent.

Example 2

A method for deeply treating oily sludge microorganisms at a well site comprises the following steps:

s1, preparing a yeast liquid, a bacillus liquid and a petroleum degrading bacteria liquid, and mixing the above bacteria liquids to obtain a mixed bacteria liquid for later use; the yeast is Saccharomyces cerevisiae CGMCC NO.12417, and the concentration of the bacterial liquid is 2 x 10⁷Per mL, the bacillus is bacillus megaterium CGMCC NO.3770, and the concentration of the bacterial liquid is8×10⁷each/mL, the petroleum degrading bacteria are BDB-n biodegradable bacteria, and the concentration of the bacteria liquid is 7 multiplied by 10⁷Per mL; the mass ratio of the saccharomycete liquid to the bacillus liquid to the petroleum degrading bacteria liquid is 1:1: 2;

will be 20m³The well site oily sludge is transferred into a storage pool 1, the depth of the storage pool 1 is 2m, the length of the storage pool is 4m, and the width of the storage pool is 4m, and then a microorganism input frame is placed into the storage pool 1;

the structure of the microorganism input frame is as follows: the microorganism input frame is formed by cross-connecting a plurality of channel tubes 2; the channel pipe 2 comprises a longitudinal pipe and a plurality of transverse pipes fixedly connected to the longitudinal pipe, a layer of transverse pipe is arranged at the height of 20cm, 4 transverse pipes are uniformly distributed around the longitudinal pipe at each layer, the length of each transverse pipe is 1.8m, each transverse pipe is communicated with the longitudinal pipe, the pipe walls of the transverse pipes and the longitudinal pipe are of net structures and are provided with a plurality of liquid outlets, and a base is arranged at the bottom of the longitudinal pipe; the upper end of the longitudinal pipeline is an open end, and the lower end of the longitudinal pipeline and the two ends of all the transverse pipelines are blind ends; the longitudinal pipeline and the transverse pipeline are both round pipes, and the inner diameter of each pipe is 3 cm;

a degradable loading membrane 3 is laid outside each of the longitudinal pipeline and the transverse pipeline, the degradable loading membrane 3 is a degradable starch film, all liquid outlets of the corresponding channel pipe 2 are closed by the degradable loading membrane 3, the inner wall of the degradable loading membrane 3 and the position between the channel pipe 2 and the degradable loading membrane 3 are loaded with 40-mesh charcoal powder, the charcoal powder can be loaded on the degradable loading membrane 3 in an adhesion mode, and the loading thickness of the charcoal powder is 0.5 cm; when the microorganism input frame is put into the storage pool 1, the open end of the channel pipe 2 is positioned outside the upper surface of the oily sludge;

s2, adding 100L of mixed bacteria liquid into the microorganism input frame from the open end of the channel pipe, allowing the bacteria liquid to flow into each channel pipe 2, standing for 20min, allowing most of bacteria in the mixed bacteria liquid to be adsorbed on charcoal, injecting high-pressure air to break the degradable load membrane 3, and allowing the materials in the channel pipe 2 to be mixed into oily sludge;

and S3, naturally fermenting for 90 days, mixing the sludge after uniform treatment, sampling and testing the oil content in the sludge to be 0.68 +/-0.11 g/100g, and the oil removal rate is ((7.33-0.68)/7.33) multiplied by 100 percent to be 90.72 percent.

Example 3

A method for deeply treating oily sludge microorganisms at a well site comprises the following steps:

s1, preparing a yeast liquid, a bacillus liquid and a petroleum degrading bacteria liquid, and mixing the above bacteria liquids to obtain a mixed bacteria liquid for later use; the yeast is Saccharomyces cerevisiae CGMCC NO.12417, and the concentration of the bacterial liquid is 2 x 10⁷Each mL, the bacillus is bacillus megaterium CGMCC NO.3770, and the concentration of the bacterial liquid is 8 multiplied by 10⁷each/mL, the petroleum degrading bacteria are BDB-a biodegradable bacteria, and the concentration of the bacteria liquid is 8 multiplied by 10⁷Per mL; the mass ratio of the saccharomycete liquid to the bacillus liquid to the petroleum degrading bacteria liquid is 1:1: 3;

will be 25m³The well site oily sludge is transferred into a storage pool 1, the depth of the storage pool 1 is 2m, the length of the storage pool is 4m, and the width of the storage pool is 4m, and then a microorganism input frame is placed into the storage pool 1;

the structure of the microorganism input frame is as follows: the microorganism input frame is formed by cross-connecting a plurality of channel tubes 2; the channel pipe 2 comprises a longitudinal pipe and a plurality of transverse pipes fixedly connected to the longitudinal pipe, a layer of transverse pipe is arranged at the height of 20cm, each layer of transverse pipe is 4, the length of each transverse pipe is 1.8m, each transverse pipe is communicated with the longitudinal pipe and uniformly distributed around the longitudinal pipe, the pipe walls of the transverse pipes and the longitudinal pipe are of net structures and are provided with a plurality of liquid outlets, and a base is arranged at the bottom of the longitudinal pipe; the upper end of the longitudinal pipeline is an open end, and the lower end of the longitudinal pipeline and the two ends of all the transverse pipelines are blind ends; the longitudinal pipeline and the transverse pipeline are both round pipes, and the inner diameter of each pipe is 3 cm;

a degradable loading membrane 3 is laid outside each of the longitudinal pipeline and the transverse pipeline, the degradable loading membrane 3 is a degradable starch film, all liquid outlets of the corresponding channel pipe 2 are closed by the degradable loading membrane 3, 100-mesh charcoal powder is loaded on the inner wall of the degradable loading membrane 3 and between the channel pipe 2 and the degradable loading membrane 3, the charcoal powder can be loaded on the degradable loading membrane 3 in an adhesion mode, and the loading thickness of the charcoal powder is 1 cm; when the microorganism input frame is put into the storage pool 1, the open end of the channel pipe 2 is positioned outside the upper surface of the oily sludge;

s2, adding 250L of mixed bacteria liquid into the microorganism input frame from the open end of the channel pipe, allowing the bacteria liquid to flow into each channel pipe 2, standing for 40min, allowing most of bacteria in the mixed bacteria liquid to be adsorbed on charcoal, injecting high-pressure air to break the degradable load membrane 3, and allowing the materials in the channel pipe 2 to be mixed into oily sludge; in this example S2, the mixed bacterial liquid was added to the microorganism input rack 2 times, and the operation was cycled by adding the mixed bacterial liquid, standing for 40min, and injecting high pressure gas until all the mixed bacterial liquid was added, and 125L of the mixed bacterial liquid was added each time.

S3, fermenting naturally for 90 days, mixing the sludge after uniform treatment, sampling and testing the oil content in the sludge to be 0.59 +/-0.07 g/100g, and the oil removal rate to be ((7.33-0.59)/7.33) multiplied by 100 percent to be 91.95 percent.

Example 4

A method for deeply treating oily sludge microorganisms at a well site comprises the following steps:

s1, preparing a yeast liquid, a bacillus liquid and a petroleum degrading bacteria liquid, and mixing the above bacteria liquids to obtain a mixed bacteria liquid for later use; the yeast is Saccharomyces cerevisiae CGMCC NO.12417, and the concentration of the bacterial liquid is 2 x 10⁷Each mL, the bacillus is bacillus megaterium CGMCC NO.3770, and the concentration of the bacterial liquid is 8 multiplied by 10⁷each/mL, the petroleum degrading bacteria is Corynebacterium glutamicum CGMCC NO.8647, and the concentration of the bacteria liquid is 7 multiplied by 10⁷Per mL; the mass ratio of the saccharomycete liquid to the bacillus liquid to the petroleum degrading bacteria liquid is 1:1: 3;

will be 20m³The oily sludge in the well site is transferred into a storage pool 1, and the storage pool 1 is deepThe temperature is 2m, the length is 4m, the width is 4m, and then a microorganism input rack is placed in the storage pool 1;

the structure of the microorganism input frame is as follows: the microorganism input frame is formed by cross-connecting a plurality of channel tubes 2; the channel pipe 2 comprises a longitudinal pipe and a plurality of transverse pipes fixedly connected to the longitudinal pipe, a layer of transverse pipe is arranged at the height of 20cm, 4 transverse pipes are uniformly distributed around the longitudinal pipe at each layer, the length of each transverse pipe is 1.8m, each transverse pipe is communicated with the longitudinal pipe, the pipe walls of the transverse pipes and the longitudinal pipe are of net structures and are provided with a plurality of liquid outlets, and a base is arranged at the bottom of the longitudinal pipe; the upper end of the longitudinal pipeline is an open end, and the lower end of the longitudinal pipeline and the two ends of all the transverse pipelines are blind ends; the longitudinal pipeline and the transverse pipeline are both round pipes, and the inner diameter of each pipe is 3 cm;

a degradable loading membrane 3 is laid outside each of the longitudinal pipeline and the transverse pipeline, the degradable loading membrane 3 is a degradable starch film, all liquid outlets of the corresponding channel pipe 2 are closed by the degradable loading membrane 3, the inner wall of the degradable loading membrane 3 and the position between the channel pipe 2 and the degradable loading membrane 3 are loaded with 40-mesh charcoal powder, the charcoal powder can be loaded on the degradable loading membrane 3 in an adhesion mode, and the loading thickness of the charcoal powder is 0.5 cm; when the microorganism input frame is put into the storage pool 1, the open end of the channel pipe 2 is positioned outside the upper surface of the oily sludge;

s2, adding 100L of mixed bacteria liquid into the microorganism input frame from the open end of the channel pipe, allowing the bacteria liquid to flow into each channel pipe 2, standing for 30min, allowing most of bacteria in the mixed bacteria liquid to be adsorbed on charcoal, injecting high-pressure air to break the degradable load membrane 3, and allowing the materials in the channel pipe 2 to be mixed into oily sludge;

s3, fermenting naturally for 75 days, mixing the sludge after uniform treatment, sampling and testing the oil content in the sludge to be 0.86 +/-0.18 g/100g, and the oil removal rate is ((7.33-0.86)/7.33) multiplied by 100 percent to be 88.27 percent.

The invention also carries out the influence of adding different microorganisms on the oil removal rate, and concretely comprises the following experimental examples.

Experimental example 1

A method for deeply treating oily sludge microorganisms at a well site, which is basically the same as the operation of the example 1, except that:

the bacterial liquid prepared in the step S1 is bacillus bacterial liquid and petroleum degrading bacterial liquid, and the mixed bacterial liquid is obtained after the bacterial liquids are mixed for later use; the bacillus is bacillus megaterium CGMCC NO.3770, and the concentration of the bacterial liquid is 8 multiplied by 10⁷each/mL, the petroleum degrading bacteria is Corynebacterium glutamicum CGMCC NO.8647, and the concentration of the bacteria liquid is 7 multiplied by 10⁷Per mL; the mass ratio of the sterile water to the bacillus liquid to the petroleum degrading bacteria liquid is 1:1:2, and the same amount of sterile water is used for replacing the saccharomycete liquid in the embodiment 1;

naturally fermenting for 60 days, mixing the sludge after uniform treatment, sampling and testing the oil content in the sludge to be 1.83 +/-0.37 g/100g, and the oil removal rate to be ((7.33-1.83)/7.33) multiplied by 100 percent to be 75.03 percent.

Experimental example 2

A method for deeply treating oily sludge microorganisms at a well site, which is basically the same as the operation of the example 1, except that:

the bacterial liquid prepared in the step S1 is yeast liquid and petroleum degrading bacterial liquid, and the bacterial liquids are mixed to obtain mixed bacterial liquid for later use; the yeast is Saccharomyces cerevisiae CGMCC NO.12417, and the concentration of the bacterial liquid is 2 x 10⁷each/mL, the petroleum degrading bacteria is Corynebacterium glutamicum CGMCC NO.8647, and the concentration of the bacteria liquid is 7 multiplied by 10⁷Per mL; the mass ratio of the yeast liquid to the sterile water to the petroleum degrading bacteria liquid is 1:1:2, and the equivalent amount of sterile water is used for replacing the bacillus liquid in the embodiment 1;

then fermenting for 60 days, mixing the sludge after uniform treatment, and sampling to test that the oil content is 1.93 plus or minus 0.08g/100g and the oil removal rate is ((7.33-1.93))/7.33 multiplied by 100% ═ 73.67%.

Experimental example 3

A method for deeply treating oily sludge microorganisms at a well site, which is basically the same as the operation of the example 1, except that:

the bacterial liquid prepared in the step S1 is petroleum degrading bacterial liquid; the petroleum degrading bacteria is Corynebacterium glutamicum CGMCC NO.8647, and the concentration of the bacteria liquid is 7 multiplied by 10⁷Per mL; the mass ratio of the sterile water to the petroleum degrading bacteria liquid is 1:1, and the equal amount of sterile water is used for replacing the sum of the mass of the bacillus liquid and the yeast liquid in the embodiment 1;

naturally fermenting for 60 days, mixing the sludge after uniform treatment, sampling and testing the oil content in the sludge to be 2.44 +/-0.21 g/100g, and the oil removal rate to be ((7.33-2.44)/7.33) multiplied by 100 percent to be 66.71 percent.

Experiments with different microorganisms degrading petroleum hydrocarbons:

mixing commercially available No. 0 diesel oil and beef extract peptone liquid culture medium according to the mass ratio of 1:4, taking 100g of mixed sample liquid, adding bacterial liquid into a 250mL triangular flask according to the following groups, carrying out shake cultivation at 35 +/-1 ℃ and 200r/min, sampling after 3d of cultivation, detecting the total petroleum hydrocarbon content, and calculating the oil removal rate (the initial total petroleum hydrocarbon content in the mixed sample liquid-the tested total petroleum hydrocarbon content in the mixed sample liquid)/the initial total petroleum hydrocarbon content in the mixed sample liquid) multiplied by 100%.

Control group: sterile water.

Group of yeasts: the yeast is Saccharomyces cerevisiae CGMCC NO.12417, and the concentration of the bacterial liquid is 2 x 10⁷one/mL.

The bacillus group: the bacillus is bacillus megaterium CGMCC NO.3770, and the concentration of the bacterial liquid is 8 multiplied by 10⁷one/mL.

Petroleum degrading bacteria group: the petroleum degrading bacteria is Corynebacterium glutamicum CGMCC NO.8647, and the concentration of the bacteria liquid is 7 multiplied by 10⁷one/mL.

Mixed bacterium group: mixing the yeast bacterial liquid, the bacillus bacterial liquid and the petroleum degrading bacterial liquid according to the mass ratio of 1:1: 2.

Triplicate replicates were made for each experimental group above and the "mean. + -. standard deviation" indicates the assay for each group. The results showed that the total petroleum hydrocarbon content of the control group, the yeast group, the bacillus group and the petroleum degrading bacteria group were degraded from 19.35g/100g to 19.33g/100g (degradation rate 0), 18.26g/100g (degradation rate 9%), 17.83g/100g (degradation rate 11%), 12.61g/100g (degradation rate 38%) and 3.48g/100g (degradation rate 85%), respectively.

It should be noted that, the connection relation of the components not specifically mentioned in the present invention is the default of the prior art, and the connection relation of the structures is not described in detail since it does not relate to the invention point and is a common application of the prior art.

It should be noted that, when the present invention relates to a numerical range, it should be understood that two endpoints of each numerical range and any value between the two endpoints can be selected, and since the steps and methods adopted are the same as those in the embodiment, in order to prevent redundancy, the present invention describes a preferred embodiment. While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all such alterations and modifications as fall within the scope of the invention.

It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.