阅读说明：本技术 玉米植株全同胞单交种的制种方法 (Method for producing seeds of corn plant holomorphic single cross hybrid ) 是由 彭绪冰 覃远照 彭绪伟 李大伟 彭绪朋 田文华 彭勇宜 刘宗坤 彭飞宜 张宏林 于 2020-07-31 设计创作，主要内容包括：本发明提供了一种玉米植株全同胞单交种的制种方法,包括：S1、筛选亲本A、亲本B及亲本C；S2、将亲本A和亲本B杂交,以得到单交种F1；S3、将单交种F1于纹枯病和茎腐病高发的田块种植,种植密度5000～6000株/亩,连续种植,并于13～15叶期接种纹枯病原菌,得纹枯病耐受性的单株；S4、将收获的单株连续多代自交选择,测定配合力,得全同胞自交系D和自交系E；S5、将全同胞自交系D与自交系E杂交,得全同胞单交种；S6、将全同胞单交种与亲本C杂交,得目的杂交种。本发明通过全同胞亲本杂交产生姊妹单交种作为母本,并用亲本做父本配制杂交种,以大幅提高制种产量,并降低了制种成本。(The invention provides a seed production method of a corn plant holomorphic single cross hybrid, which comprises the following steps: s1, screening a parent A, a parent B and a parent C; s2, hybridizing the parent A and the parent B to obtain a single cross variety F1; s3, planting the single cross hybrid F1 in a field with high banded sclerotial blight and stem rot, continuously planting the single cross hybrid at a planting density of 5000-6000 plants/mu, and inoculating banded sclerotial blight pathogenic bacteria at a 13-15 leaf stage to obtain a single plant with banded sclerotial blight tolerance; s4, carrying out continuous multi-generation selfing selection on the harvested single plants, and measuring the combining ability to obtain a homoeocellular inbred line D and an inbred line E; s5, hybridizing the full-sib inbred line D and the inbred line E to obtain a full-sib single hybrid; and S6, hybridizing the homoblast single hybrid with the parent C to obtain the target hybrid. In the invention, sister single hybrid seeds generated by hybridization of the full-sib parents are used as female parents, and the parents are used as male parents to prepare hybrid seeds, so that the seed production yield is greatly improved, and the seed production cost is reduced.)

1. A seed production method of a corn plant holomorphic single cross hybrid is characterized by comprising the following steps:

s1, screening a parent A, a parent B and a parent C;

s2, hybridizing the parent A and the parent B to obtain a single cross variety F1;

s3, planting the single cross hybrid F1 in a field with high banded sclerotial blight and stem rot, continuously planting the single cross hybrid at a planting density of 5000-6000 plants/mu, and inoculating banded sclerotial blight pathogenic bacteria at a 13-15 leaf stage to obtain a single plant with banded sclerotial blight tolerance;

s4, carrying out continuous multi-generation selfing selection on the harvested single plants, and measuring the combining ability to obtain a homoeocellular inbred line D and an inbred line E;

s5, hybridizing the full-sib inbred line D and the inbred line E to obtain a full-sib single hybrid;

and S6, hybridizing the homoblast single hybrid with the parent C to obtain the target hybrid.

2. The method for producing a fully sib hybrid maize plant according to claim 1, wherein in step S3, the single hybrid F1 is planted in the natural environment of the south.

3. The method for producing a homoblast inbred maize plant of claim 2, wherein in step S3, the soil moisture in said field is 60-80% RH.

4. The method for producing a fully homozygote maize plant seed of claim 3, wherein the harvested individual is selfed continuously for at least 7 generations in step S4.

5. The method for producing a inbred of a homoblast maize plant of claim 4, wherein in step S4, said steps of selecting the harvested inbred plants for successive multiple selfings, and determining the combining ability to obtain homoblast inbred line D and inbred line E comprise:

in the third generation selfing seed planting and the fourth generation selfing seed planting, hybridizing all ears to determine the combining ability, and screening a selfing line D with strong disease resistance and high combining ability;

in the fifth generation selfing seed planting, incomplete double-row hybridization is carried out on all ear rows to determine combining ability, and a selfing line E with heterosis is screened to obtain a full-sib selfing line D and a selfing line E.

6. The method for producing a inbred of a homoblast maize plant of claim 4, wherein in step S4, said steps of selecting the harvested inbred plants for successive multiple selfings, and determining the combining ability to obtain homoblast inbred line D and inbred line E comprise:

in the third generation inbred seed planting, the fourth generation inbred seed planting and the fifth generation inbred seed planting, all ears are hybridized to determine the combining ability, and an inbred line D with strong disease resistance and high combining ability is screened;

in the sixth generation of inbred planting, incomplete double row crosses were made to all ears to determine combining ability, and inbred line E with hybrid vigor was selected, which was obtained from inbred line D and inbred line E.

Technical Field

The invention relates to the technical field of crop planting, in particular to a seed production method of a corn plant holomorphic single cross hybrid.

Background

Corn is a world important crop, second only to wheat in its overall yield, and second in place. The application of corn hybrid in production is one of the important means for improving the corn yield. The quality of corn seeds is the key to influence the quality of commercial corn. In the production and breeding process of hybrid seeds, the practical problems that the uniformity, purity, bud rate and bud potential of the seeds do not reach the standard always exist. The method mainly has the key technical links of hybrid seed production, such as inadequate management and the like, so that the seed production technology of the corn directly influences the quality of the corn seeds in the hybrid seed production process of a breeding unit, provides high-quality corn seeds for corn production, improves the seed production yield, reduces the seed production cost, and is imperative.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides a method for producing seeds of a full-sib single-cross hybrid of a corn plant, which aims to solve the technical problem of low yield of the seeds produced by the traditional seed production method in the related technology.

The invention provides a seed production method of a corn plant holomorphic single cross hybrid, which comprises the following steps:

s1, screening a parent A, a parent B and a parent C;

s2, hybridizing the parent A and the parent B to obtain a single cross variety F1;

s3, planting the single cross hybrid F1 in a field with high banded sclerotial blight and stem rot, continuously planting the single cross hybrid at a planting density of 5000-6000 plants/mu, and inoculating banded sclerotial blight pathogenic bacteria at a 13-15 leaf stage to obtain a single plant with banded sclerotial blight tolerance;

s4, carrying out continuous multi-generation selfing selection on the harvested single plants, and measuring the combining ability to obtain a homoeocellular inbred line D and an inbred line E;

s5, hybridizing the full-sib inbred line D and the inbred line E to obtain a full-sib single hybrid;

and S6, hybridizing the homoblast single hybrid with the parent C to obtain the target hybrid.

Optionally, in the step S3, the planting environment of the single cross hybrid F1 is planting in a natural environment in the south.

Optionally, in the step S3, the moisture content of the soil in the field is 60-80% RH.

Optionally, in said step S4, the harvested individuals are selfed continuously for at least 7 generations.

Optionally, in step S4, the steps of selecting harvested single plants by continuous multi-generation selfing, and determining combining ability to obtain inbred lines D and E of the same cell include:

in the third generation selfing seed planting and the fourth generation selfing seed planting, hybridizing all ears to determine the combining ability, and screening a selfing line D with strong disease resistance and high combining ability;

in the fifth generation selfing seed planting, incomplete double-row hybridization is carried out on all ear rows to determine combining ability, and a selfing line E with heterosis is screened to obtain a full-sib selfing line D and a selfing line E.

Optionally, in step S4, the steps of selecting harvested single plants by continuous multi-generation selfing, and determining combining ability to obtain inbred lines D and E of the same cell include:

in the third generation inbred seed planting, the fourth generation inbred seed planting and the fifth generation inbred seed planting, all ears are hybridized to determine the combining ability, and an inbred line D with strong disease resistance and high combining ability is screened;

in the sixth generation of inbred planting, incomplete double row crosses were made to all ears to determine combining ability, and inbred line E with hybrid vigor was selected, which was obtained from inbred line D and inbred line E.

Compared with the prior art, the invention has the following beneficial effects:

in the technology of the invention, the homoblast single hybrid is generated by hybridizing the homoblast inbred line parents as the female parent, and the inbred line parents are used as the male parent to prepare the hybrid, so that the seed production yield is greatly improved, and the seed production cost is reduced.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clearly apparent, the technical solutions of the present invention are further described below with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

The invention provides a seed production method of a corn plant holomorphic single cross hybrid, which comprises the following steps:

s1, screening a parent A, a parent B and a parent C;

s2, hybridizing the parent A and the parent B to obtain a single cross variety F1;

s3, planting the single cross hybrid F1 in a field with high banded sclerotial blight and stem rot, continuously planting the single cross hybrid at a planting density of 5000-6000 plants/mu, and inoculating banded sclerotial blight pathogenic bacteria at a 13-15 leaf stage to obtain a single plant with banded sclerotial blight tolerance;

s4, carrying out continuous multi-generation selfing selection on the harvested single plants, and measuring the combining ability to obtain a homoeocellular inbred line D and an inbred line E;

s5, hybridizing the full-sib inbred line D and the inbred line E to obtain a full-sib single hybrid;

and S6, hybridizing the homoblast single hybrid with the parent C to obtain the target hybrid.

Optionally, in the step S3, the planting environment of the single cross hybrid F1 is planting in a natural environment in the south.

Optionally, in the step S3, the moisture content of the soil in the field is 60-80% RH.

Optionally, in said step S4, the harvested individuals are selfed continuously for at least 7 generations.

Optionally, in step S4, the steps of selecting harvested single plants by continuous multi-generation selfing, and determining combining ability to obtain inbred lines D and E of the same cell include:

in the third generation selfing seed planting and the fourth generation selfing seed planting, hybridizing all ears to determine the combining ability, and screening a selfing line D with strong disease resistance and high combining ability;

in the fifth generation selfing seed planting, incomplete double-row hybridization is carried out on all ear rows to determine combining ability, and a selfing line E with heterosis is screened to obtain a full-sib selfing line D and a selfing line E.

Optionally, in step S4, the steps of selecting harvested single plants by continuous multi-generation selfing, and determining combining ability to obtain inbred lines D and E of the same cell include:

in the third generation inbred seed planting, the fourth generation inbred seed planting and the fifth generation inbred seed planting, all ears are hybridized to determine the combining ability, and an inbred line D with strong disease resistance and high combining ability is screened;

in the sixth generation of inbred planting, incomplete double row crosses were made to all ears to determine combining ability, and inbred line E with hybrid vigor was selected, which was obtained from inbred line D and inbred line E.

In order to more clearly illustrate the seed production effect of the seed production method of the maize plant homoblast single cross, the following example groups are selected for detailed explanation. It should be understood that the following example sets are only for illustrating the effect of the seed production method of the present invention and do not limit the seed production method of the present invention.