阅读说明：本技术 一种基于原花青素低聚体的面部激素依赖性皮炎治疗液及其制备方法 (Procyanidine oligomer-based facial hormone-dependent dermatitis treatment liquid and preparation method thereof ) 是由 曹原 眭春 王启名 眭新 曹珊 周兰 王铁军 于 2020-08-17 设计创作，主要内容包括：本发明公开了一种基于原花青素低聚体的面部激素依赖性皮炎治疗液及其制备方法。面部激素依赖性皮炎治疗液主要由保湿剂、粘稠剂、去离子水、生物多肽、pH调节剂、原花青素复合液、维生素E混配制得；其中生物多肽为十三肽与谷胱甘肽的混合物,原花青素复合液由原花青素复合液主要由原花青素低聚体、海藻糖、壳聚糖、芦荟提取液、薄荷油经反应制得。原花青素低聚体是一种天然化学物质,具有抗炎、抗敏、消除体内自由基、抗氧化等特性,而壳聚糖具有优异的抗菌功能；将原花青素低聚体与壳聚糖结合,再加入薄荷油可降低灼烧和疼痛感；最终制得面部激素依赖性皮炎治疗液；该治疗液使用方法简单,无毒副作用,治疗效果优异。(The invention discloses a facial hormone-dependent dermatitis treatment liquid based on procyanidine oligomers and a preparation method thereof. The facial hormone-dependent dermatitis treatment liquid is mainly prepared by mixing a humectant, a viscous agent, deionized water, biological polypeptide, a pH regulator, a procyanidine composite liquid and vitamin E; the biological polypeptide is a mixture of tridecapeptide and glutathione, and the procyanidin complex liquid is prepared by reacting procyanidin oligomer, trehalose, chitosan, aloe extract and peppermint oil. Procyanidin oligomer is a natural chemical substance, has the characteristics of resisting inflammation, resisting allergy, eliminating in-vivo free radicals, resisting oxidation and the like, and chitosan has an excellent antibacterial function; combining procyanidin oligomer with chitosan, and adding oleum Menthae Dementholatum to reduce burning and pain; finally preparing the facial hormone-dependent dermatitis treatment liquid; the therapeutic liquid has the advantages of simple application method, no adverse side effects, and good therapeutic effect.)

1. A facial hormone-dependent dermatitis therapeutic solution based on procyanidin oligomers is characterized in that; the raw material components comprise 1.0-5.0% of humectant, 0.1-0.3% of viscous agent, 13-17% of biological polypeptide, 0.05-0.35% of pH regulator, 1.5-2.5% of procyanidine complex liquid, 0.06-0.12% of vitamin E and the balance of deionized water.

2. The proanthocyanidin oligomer-based therapeutic solution for facial hormone-dependent dermatitis as claimed in claim 1, wherein: the procyanidin compound liquid is prepared from procyanidin oligomer, trehalose, chitosan, aloe extract and oleum Menthae Dementholatum by reacting.

3. The proanthocyanidin oligomer-based therapeutic solution for facial hormone-dependent dermatitis as claimed in claim 1, wherein: the biological polypeptide is a mixture of tridecapeptide and glutathione.

4. The proanthocyanidin oligomer-based therapeutic solution for facial hormone-dependent dermatitis as claimed in claim 1, wherein: the humectant is any one of propylene glycol, polyethylene glycol, glycerol and hyaluronic acid, and preferably, the humectant is a mixed solution of hyaluronic acid, glycerol and polyethylene glycol.

5. The proanthocyanidin oligomer-based therapeutic solution for facial hormone-dependent dermatitis as claimed in claim 1, wherein: the thickening agent is any one or more of carbomer, xanthan gum, hydroxyethyl cellulose, methyl cellulose, hydroxypropyl methyl cellulose, propylene glycol alginate and acrylate, and preferably the thickening agent is carbomer.

6. The proanthocyanidin oligomer-based therapeutic solution for facial hormone-dependent dermatitis as claimed in claim 1, wherein: the pH regulator is any one of citric acid, potassium hydroxide, lactic acid and triethanolamine, and preferably the pH regulator is triethanolamine.

7. The proanthocyanidin oligomer-based therapeutic solution for facial hormone-dependent dermatitis as claimed in claim 1, wherein: preferably, the raw material components are as follows:

8. a preparation method of facial hormone-dependent dermatitis treatment liquid based on procyanidine oligomers is characterized by comprising the following steps: comprises the following steps of (a) carrying out,

(1) dissolving procyanidine oligomer in ethanol solution, and adding lipase to obtain solution A; dissolving chitosan in an ethanol solution, dropwise adding a sodium hydroxide solution, adding a hydrogen peroxide solution, stirring, reacting, heating, dropwise adding the solution A, standing for reacting after dropwise adding is finished, separating to obtain a solid phase, washing with absolute ethanol, adding trehalose and deionized water, and stirring at a high speed to obtain a solution B; heating the aloe extract, adding the solution B, dispersing at high speed, cooling to room temperature, dropwise adding peppermint oil, and stirring to obtain procyanidin composite liquid;

(2) mixing deionized water, thickening agent and humectant, stirring, and homogenizing in homogenizer; adding biological polypeptide, procyanidin complex solution, and vitamin E, stirring at high speed, adding pH regulator dropwise, stirring, and naturally cooling to room temperature; sterilizing to obtain the finished product of the facial hormone-dependent dermatitis treatment liquid based on the procyanidine oligomer.

9. The method of preparing a proanthocyanidin oligomer-based therapeutic solution for facial hormone-dependent dermatitis as claimed in claim 8, wherein: comprises the following steps of (a) carrying out,

(1) dissolving procyanidin oligomer in ethanol solution, adding lipase and trehalose, and stirring for 8-10min to obtain solution A; dissolving chitosan in ethanol solution, dropwise adding sodium hydroxide solution until pH is 8.0-10.0, adding hydrogen peroxide solution, stirring, reacting for 10-20min, heating to 42-47 deg.C, dropwise adding solution A, and standing for 40-60 min; adding catalase, stirring, separating to obtain a solid phase, washing with absolute ethanol, adding trehalose and deionized water, and stirring at a high speed of 800 r/min; obtaining a solution B; heating Aloe extractive solution to 55-60 deg.C, adding solution B, dispersing at high speed, cooling to room temperature, adding oleum Menthae Dementholatum dropwise, stirring for 8-10min to obtain procyanidin complex solution;

(2) mixing deionized water, viscous agent and humectant, stirring at 300-500r/min for 5-6min, and homogenizing in homogenizer for 8-15 min; adding biological polypeptide, procyanidin complex solution, and vitamin E, stirring at high speed of 500r/min and 65-70 deg.C for 5-6min, dropwise adding pH regulator, stirring for 20-30min, and naturally cooling to room temperature; sterilizing to obtain the finished product of the facial hormone-dependent dermatitis treatment liquid based on the procyanidine oligomer.

Technical Field

The invention relates to the technical field of cosmetics, in particular to a facial hormone-dependent dermatitis treatment liquid based on procyanidine oligomers and a preparation method thereof.

Background

The facial hormone-dependent dermatitis is a common dermatological disease, and clinical symptoms are mostly manifested by thinning of stratum corneum, atrophy, flushing, telangiectasia, acne, rosacea and the like, and are accompanied by a little of thin crumbs, pigmentation, dryness, itching and burning touch. The causes of facial hormone-dependent dermatitis are roughly the following: 1. the medicine is improperly selected, and the patient can select the medicine containing the hormone by himself without the guidance of a doctor; 2. the medicine is not properly taken, the administration time is too long, and the skin barrier function can be damaged by long-term use of hormone medicines, so that the hormone medicines generate dependence on the medicines, and malignant circulation is caused; 3. in order to meet the expectation of beauty of patients, a beauty institution uses a large amount of hormone medicines, and can show the effects of whitening and beautifying in a short time; but the long-term use of the medicine can form dependence to cause the disease. The treatment period of the facial hormone-dependent dermatitis is long, the disease condition is easy to repeat, the facial beauty is affected, and the physical and psychological health of a patient is harmed.

The traditional Chinese medicine considers that hormone medicines are externally used for a long time and accumulated on skin, and can help yang to transform heat, heat injure blood collaterals, consume body fluid and injure yin; the external application of indigo naturalis ointment, cortex Phellodendri ointment and radix Angelicae sinensis ointment is mostly adopted in treatment, the effect is not obvious, and the repeated application is easy. The western medicine for treating the facial hormone-dependent dermatitis mostly adopts medicines such as calcineurin inhibitors, non-steroidal medicines, heparin sodium ointment and the like for local treatment, and the treatment method has certain treatment effect, but can bring sleepiness, and can bring burning pain, dryness and desquamation discomfort when being externally used. Therefore, it is of value to study a pharmaceutical preparation which is effective in treating facial hormone-dependent dermatitis, safe and harmless, and has little side effects.

At present, the procyanidin-containing external treatment liquid for treating the facial hormone-dependent dermatitis is less, but the invention provides the external treatment liquid for the facial hormone-dependent dermatitis, which takes procyanidin oligomers as main raw materials, and the external treatment liquid has the advantages of simple use method, excellent treatment effect and no toxic or side effect; has been successfully applied to clinical treatment by various hospitals and has achieved good treatment effect.

Disclosure of Invention

The invention aims to provide a facial hormone-dependent dermatitis treatment liquid based on procyanidine oligomers and a preparation method thereof, so as to solve the problems in the background technology.

In order to solve the technical problems, the invention provides the following technical scheme: a facial hormone-dependent dermatitis therapeutic liquid based on procyanidin oligomer comprises humectant 1.0-5.0%, viscous agent 0.1-0.3%, biological polypeptide 13-17%, pH regulator 0.05-0.35%, procyanidin complex liquid 1.5-2.5%, vitamin E0.06-0.12%, and deionized water in balance.

Further, the procyanidin compound liquid is mainly prepared by reacting procyanidin oligomer, trehalose, chitosan, aloe extract and peppermint oil, wherein the addition amount of the peppermint oil is 0.01% of the total amount.

Further, the biological polypeptide is a mixture of tridecapeptide and glutathione; the mass ratio of the tridecapeptide to the glutathione is 2: 1; according to the scheme, the tridecapeptide and the glutathione are added to promote the healing of the acne and repair the skin barrier; the skin care product can provide amino acids and other nutrients to the skin during treatment, and promote the skin to quickly recover to a healthy state.

Further, the humectant is any one of propylene glycol, polyethylene glycol, glycerin and hyaluronic acid, preferably, the humectant is a mixed solution of hyaluronic acid, glycerin and polyethylene glycol.

Further, the thickening agent is any one or more of carbomer, xanthan gum, hydroxyethyl cellulose, methyl cellulose, hydroxypropyl methyl cellulose, propylene glycol alginate and acrylate, and preferably the thickening agent is carbomer.

Further, the pH adjusting agent is any one of citric acid, potassium hydroxide, lactic acid, and triethanolamine, and preferably, the pH adjusting agent is triethanolamine.

Further, the facial hormone-dependent dermatitis treatment liquid based on the procyanidine oligomer comprises the following raw material components:

a method for preparing facial hormone-dependent dermatitis therapeutic solution based on procyanidin oligomer comprises: comprises the following steps of (a) carrying out,

(1) dissolving procyanidine oligomer in ethanol solution, and adding lipase to obtain solution A; dissolving chitosan in an ethanol solution, dropwise adding a sodium hydroxide solution, adding a hydrogen peroxide solution, wherein the mass ratio of the hydrogen peroxide solution to lipase is 5:1, stirring, reacting, heating, dropwise adding the solution A, standing for reacting after dropwise adding, separating to obtain a solid phase, washing with absolute ethanol, adding trehalose and deionized water, and stirring at a high speed to obtain a solution B; heating the aloe extract, adding the solution B, dispersing at high speed, cooling to room temperature, dropwise adding peppermint oil, and stirring to obtain procyanidin composite liquid;

(2) mixing deionized water, thickening agent and humectant, stirring, and homogenizing in homogenizer; adding biological polypeptide, procyanidin complex solution, and vitamin E, stirring at high speed, adding pH regulator dropwise, stirring, and naturally cooling to room temperature; sterilizing to obtain the finished product of the facial hormone-dependent dermatitis treatment liquid based on the procyanidine oligomer.

Further, a preparation method of the facial hormone-dependent dermatitis treatment liquid based on procyanidine oligomers comprises the following steps;

(1) dissolving procyanidin oligomer in ethanol solution, adding lipase and trehalose, wherein the addition amount of trehalose is 50% of that of procyanidin oligomer, adding trehalose in two times, and adding 1/10 trehalose. Stirring for 8-10min to obtain solution A; dissolving chitosan in ethanol solution, dropwise adding sodium hydroxide solution until pH is 8.0-10.0, adding hydrogen peroxide solution, stirring, reacting for 10-20min, heating to 42-47 deg.C, dropwise adding solution A, and standing for 40-60 min; adding catalase, stirring, separating to obtain a solid phase, washing with absolute ethanol, adding the residual 9/10 trehalose and deionized water, and stirring at a high speed of 800 r/min; obtaining a solution B; heating Aloe extractive solution to 55-60 deg.C, adding solution B, dispersing at high speed, cooling to room temperature, adding oleum Menthae Dementholatum dropwise, stirring for 8-10min to obtain procyanidin complex solution;

the procyanidin oligomer is a natural substance extracted from plants, has excellent inoxidizability, in-vivo free radical scavenging, anti-inflammatory, antibacterial and other effects, but has very active property and is unstable under the conditions of illumination, temperature, metal ions and the like due to the fact that the procyanidin oligomer contains a plurality of phenolic hydroxyl groups, so that the application efficiency of the procyanidin oligomer is reduced; the chitosan is also a natural substance, has natural antibacterial and bacteriostatic effects, has an inhibiting effect on gram-positive bacteria, gram-negative bacteria, candida albicans and other strains, can capture heavy metal ions such as lead, mercury and arsenic to form a chelate with the heavy metal ions, and avoids the pollution of the heavy metal to the skin; but the bacteriostatic effect is not obvious when the chitosan is used alone. The technical scheme effectively combines the procyanidine oligomer and the chitosan, improves respective defects and enables the two substances to exert the maximum effect. Under the action of a catalyst, procyanidin oligomer and chitosan are subjected to polymerization reaction and are connected by covalent bonds to form a polymer. The catalyst is hydrogen peroxide and lipase with the mass ratio of 5:1, the combination of the hydrogen peroxide and the lipase has higher catalytic efficiency than the single use of the hydrogen peroxide, and the polymerization of procyanidine oligomer and chitosan is promoted more quickly and thoroughly; when lipase is added, a small amount of trehalose is used in a matching manner, and the trehalose serving as a natural preservative can be used as a stable medium of the lipase, so that the activity and stability of the lipase can be improved, and the catalytic effect is ensured. After the polymerization reaction is finished, the hydrogen peroxide is remained in the reaction liquid; because the hydrogen peroxide has oxidability, the skin can be burnt when the skin is wiped on a wound, and the skin can be burnt by serious people; therefore, according to the technical scheme, catalase is added after the polymerization reaction is finished, and the catalase is decomposed to avoid skin burning sensation and other injuries brought by the catalase. The polymer formed by procyanidin oligomer and chitosan has the characteristics of two compounds, has excellent oxidation resistance, free radical removal, anti-inflammation, anti-allergy, antibacterial and bacteriostatic effects, and can be used for treating and preventing symptoms such as cuticle thinning, atrophy, flushing, telangiectasia, acne and the like when being applied to an affected part of hormone-dependent dermatitis. Trehalose is a natural saccharide and has a magical protective effect on organisms; after procyanidine oligomer and chitosan form a polymer, adding trehalose, wherein the trehalose structure has an oxygen six-membered ring, and hydroxyl methoxyl groups on the oxygen six-membered ring can perform saccharification reaction with anthocyanin in procyanidine to form a stable complex; thereby further improving the stability of the polymer and ensuring the treatment effect of the treatment solution.

Natural substances extracted from fresh stems and leaves of Mentha arvensis can be used for skin or mucosa to produce cooling feeling to relieve discomfort and pain. The scheme adds a trace amount of peppermint oil, and can reduce burning and pain of sensitive skin in the treatment process while not influencing the treatment effect.

The vitamin E, the carbomer, the glycerol and the polyethylene glycol have good mixing effect with the skin, and a layer of wet repairing film can be formed on the surface of the skin; the skin care product can isolate foreign substances from invading the skin, lock skin moisture, prevent evaporation, and improve the phenomena of dry skin and desquamation of patients.

(2) Mixing deionized water, viscous agent and humectant, stirring at 300-500r/min for 5-6min, and homogenizing in homogenizer for 8-15 min; adding biological polypeptide, procyanidin complex solution, and vitamin E, stirring at high speed of 500r/min and 65-70 deg.C for 5-6min, dropwise adding pH regulator, stirring for 20-30min, and naturally cooling to room temperature; sterilizing to obtain the finished product of the facial hormone-dependent dermatitis treatment liquid based on the procyanidine oligomer.

Compared with the prior art, the invention has the following beneficial effects: the core component procyanidin mixed solution of the treatment solution is obtained by reacting procyanidin oligomer, trehalose, chitosan, aloe extract and peppermint oil, has excellent functions of eliminating free radicals, resisting inflammation, allergy, bacteria and bacteria, and can relieve edema, protect capillary vessels, inhibit the activity of inflammatory factors and improve allergic symptoms; adding trace amount of oleum Menthae Dementholatum to relieve burning and pain during application; furthermore, biological polypeptide and vitamin E further repair the skin to form a moisturizing and water-locking layer. Raw materials selected from procyanidine oligomer, trehalose, chitosan, aloe extract and peppermint oil in the treatment liquid belong to natural pollution-free substances, and no preservative component is added, so that the product is high in safety and simple in use method; there have been many successful clinical treatment cases.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.