阅读说明：本技术 尿中酮体检测用试验片 (Test piece for detecting ketone body in urine ) 是由 石田晶子 大畑和夫 于 2019-02-21 设计创作，主要内容包括：在尿中酮体测定中,抑制起因于具有巯基的药剂的假阳性。通过向尿中酮体检测用试验片添加氧化剂,抑制起因于具有巯基的药剂的假阳性。(In the urine ketone body assay, false positives due to agents having thiol groups are inhibited. False positives due to agents having thiol groups are suppressed by adding an oxidizing agent to a test piece for detecting ketone bodies in urine.)

1. A test piece for detecting a ketone body in urine, which contains an oxidizing agent.

2. The test piece for detecting a ketone body in urine according to claim 1, wherein the oxidizing agent is an oxidizing agent for suppressing a false positive.

3. The test piece for detecting a ketone body in urine as claimed in claim 1 or 2, wherein the oxidizing agent is potassium iodate.

4. A method for suppressing false positive, characterized in that an oxidizing agent is added to a test piece for detecting a ketone body in urine.

5. The method of false positive inhibition of claim 4, wherein the oxidizing agent is potassium iodate.

[ technical field ] A method for producing a semiconductor device

The present invention relates to the inhibition of non-specific reactions in the measurement of ketone bodies in urine.

[ background of the invention ]

The detection of various components in urine is used for diagnosis of various diseases, particularly kidney diseases, urinary tract diseases, and diabetes. The collection of urine specimen imposes little burden on the subject, and urine examination is an examination that needs to be performed not only for the examinees in hospitals but also for the health of the examinees.

Urine test paper used for testing a urine specimen generally has a shape in which a plurality of test pieces capable of detecting various components in urine by color reaction are attached to a support made of synthetic resin such as PET. The urine test paper is dipped in a urine specimen collected from a paper cup or the like, and then the specimen is lifted up, and the presence or absence of each component is determined qualitatively (positive or negative) by visually confirming the color development of the specimen portion. In the test using the fully automatic analyzer, a urine specimen was dropped onto a urine test paper to optically detect the color development of the test piece, and the color development was applied to a calibration curve to calculate the concentrations of the respective components.

Examples of items that can be detected with urine test paper include glucose, proteins, occult blood, and ketone bodies. In addition, creatinine can be measured, and the daily urine protein mass can be calculated from the urine protein concentration at any time.

Ketone bodies are a generic term for acetone, acetoacetic acid, and β -hydroxytyrosic acid, and are not usually detectable in the urine of healthy people. However, it is known that in diabetes, hyperthyroidism, and the like, ketone bodies in urine become positive.

The detection principle of ketone body in urine utilizes the reaction of sodium nitroprusside and acetone or acetoacetic acid to make them be purple. However, sodium nitroprusside reacts similarly to a compound having a mercapto group to give a purple color. That is, when a subject takes a drug containing a compound having a mercapto group, the drug is excreted into urine and reacts with sodium nitroprusside, and the result of examination of ketone bodies in urine becomes false positive.

As such agents having a mercapto group, which cause a ketone body in urine to be a false positive, captopril (antihypertensive agent) or bucindoramin (anti-rheumatoid agent) is known. In particular, proteinuria is a side effect of busilamine, and therefore, frequent urine qualitative tests in busilamine-treated patients have caused a problem that ketone bodies in urine become false positives.

[ SUMMARY OF THE INVENTION ]

[ problem to be solved by the invention ]

The problem to be solved by the present invention is to suppress false positive caused by a drug having a mercapto group in the measurement of ketone bodies in urine.

[ MEANS FOR SOLVING PROBLEMS ] to solve the problems

The present inventors have made intensive studies to solve the above problems, and have completed the following inventions.

The present invention provides the following test piece for detecting ketone bodies in urine and a method for suppressing false positive.

(1) A test piece for detecting a ketone body in urine, which contains an oxidizing agent.

(2) The test piece for detecting a ketone body in urine according to (1), wherein the oxidizing agent is an oxidizing agent for suppressing false positive.

(3) The test piece for detecting a ketone body in urine according to (1) or (2), wherein the oxidizing agent is potassium iodate.

(4) A method for suppressing false positive, characterized by adding an oxidizing agent to a test piece for detecting a ketone body in urine.

(5) The method of inhibiting false positive according to (4), wherein the oxidizing agent is potassium iodate.

[ Effect of the invention ]

The test piece for detecting a ketone body in urine of the present invention contains an oxidizing agent, and therefore, even when a test subject in urine test takes a drug having a mercapto group, the test result can be suppressed from being false positive.

[ detailed description ] embodiments

The test piece for detecting a ketone body in urine of the present invention is prepared as a test piece containing a reagent for detecting a ketone body, as described in detail below. Further, the test piece of another test object item may be provided on the support and may be configured as a urine test paper for multi-item test, or may be configured as a test paper for detecting only a single item of ketone body in urine.

The test piece for detecting a ketone body in urine of the present invention contains a reagent composition containing sodium nitroprusside and an oxidizing agent. In the test piece for detecting a ketone body in urine of the present invention, the oxidizing agent sodium nitroprusside reacts with the compound having a mercapto group, whereby non-specific color development can be suppressed to make the test piece false positive. The oxidizing agent is not particularly limited, and an alkali metal salt of iodic acid or potassium ferricyanide may be used. Among these oxidizing agents, potassium iodate is preferred.

The test piece for detecting a ketone body in urine of the present invention is produced by preparing a solution containing sodium nitroprusside and an oxidizing agent, impregnating the solution in an absorbent carrier, and then drying the impregnated solution. The concentration of sodium nitroprusside in the solution is preferably 0.5% to 1.5%. The concentration of the oxidizing agent in the solution is preferably 0.25% to 0.75%.

As the reagent composition to be loaded on the test piece, in addition to sodium nitroprusside and the oxidizing agent, a solubilizing agent, a sensitizer, a wetting agent, a buffer, or the like can be used.

As the absorbent carrier impregnated with the reagent composition, filter paper is widely used, and cotton, nonwoven fabric, glass fiber and the like can be used, and the reagent composition can further contain an organic polymer such as gelatin, synthetic resin and the like.

The thus-produced test piece for detecting a ketone body in urine may be attached to a support, or may be attached to a support together with a test piece for detecting another detection target item. As the support, for example, a support made of a plastic sheet of polyvinyl chloride, polyethylene terephthalate, polypropylene, polystyrene, polyester, or the like can be used.

The test paper thus obtained was dipped in a liquid sample containing urine and immediately lifted. Further, the color development after a certain period of time was visually compared with a previously prepared standard color chart, and a qualitative or semi-quantitative test of the sample was performed. Alternatively, a quantitative test may be performed in which reflectance or the like is optically measured using a measuring device and the reflectance or the like is applied to a calibration curve to determine the concentration.