阅读说明：本技术 用于提高msms置信度的dm-swath获取 (DM-SWATH acquisition for improving MSMS confidence ) 是由 Y·勒布朗 B·西尔 于 2019-01-08 设计创作，主要内容包括：在DM-SWATH中,多个CoV和前驱物离子质量范围被接收。处理器针对多个CoV中的每个CoV执行重复的一系列步骤。针对多个CoV中的每个CoV,CoV被施加到DMS设备以选择前驱物离子的组。质量过滤器被指示以选择组中的在前驱物离子质量范围内的前驱物离子,从而产生前驱物离子的子组。分裂设备被指示以对前驱物离子的子组进行分裂,从而产生产物离子的组。质量分析仪被指示以测量产物离子的组的强度和m/z,从而产生针对多个CoV中的每个CoV的产物离子谱。DM-SWATH还被用于验证已知化合物是否在样品中。(In DM-SWATH, multiple CoV and precursor ion mass ranges are received. The processor performs a repetitive series of steps for each of the plurality of covs. For each CoV of the plurality of covs, the CoV is applied to a DMS apparatus to select a set of precursor ions. The mass filter is instructed to select precursor ions in the set that are within a mass range of the precursor ions to produce a subset of the precursor ions. A fragmentation device is instructed to fragment a subset of the precursor ions to produce a set of product ions. The mass analyzer is instructed to measure the intensities and m/z of the set of product ions to produce a product ion spectrum for each of the plurality of covs. DM-SWATH was also used to verify whether a known compound was in the sample.)

1. A system for controlling a Differential Mobility Spectrometry (DMS) device and a tandem mass spectrometer to sequentially select different groups of precursor ions having different differential mobilities and mass filter, fragment, and mass analyze the resulting product ions of each group, the system comprising:

a DMS device configured to separate precursor ions based on a compensation voltage CoV;

a tandem mass spectrometer that receives the separated precursor ions from the DMS device and that includes a mass filter and fragmentation device for filtering and fragmenting precursor ions and a mass analyzer for mass analyzing the resulting product ions; and

a processor in communication with the DMS device and the tandem mass spectrometer, the processor:

(a) receiving a plurality of CoVs for the DMS device and a precursor ion mass range for the mass filter, an

(b) For each of the plurality of covs,

applying the each CoV to the DMS device to select a set of precursor ions,

instructing the mass filter to select precursor ions in the set that are within the mass range of precursor ions, thereby generating a subset of precursor ions,

instructing the fragmentation device to fragment the subset of precursor ions, thereby generating a set of product ions, an

Instructing the mass analyzer to measure the intensities and mass-to-charge ratios, m/z, of the set of product ions, thereby generating a product ion spectrum for each of the plurality of covs.

2. The system of claim 1, wherein the DMS device is further configured to separate precursor ions based on an RF separation voltage SV, the processor further receiving an SV for the DMS device in step (a), and the processor further applying the SV to the DMS device in step (b).

3. The system of claim 2, wherein the SV is greater than 3000V.

4. The system of claim 2, wherein the reduced electric field E/N is greater than 100 Td.

5. The system of claim 1, further comprising:

a sample separation device that separates one or more compounds from a sample over time, an

An ion source that ionizes the separated one or more compounds and transmits the ionized precursor ions as an ion beam to the DMS device,

wherein the processor:

instructing the DMS device to sample the ion beam over a series of time periods, an

For each period in the series of periods,

instructing the DMS device and tandem mass spectrometer to perform a precursor survey scan without differential mobility selection by applying a CoV of zero to the DMS device, instructing the mass filter to select precursor ions in the ion beam that are within the mass range of precursor ions thereby generating a filtered set of precursor ions, instructing the fragmentation device to transmit the non-fragmented filtered set of precursor ions to the mass analyzer, and instructing the mass analyzer to measure the intensities and m/z of the filtered set of precursor ions thereby generating a precursor ion spectrum, and

instructing the DMS device and tandem mass spectrometer to perform a sequential differential mobility dependent product ion scan on the ion beam by performing step (b), an

Verifying the presence of a compound of the one or more compounds in the sample by:

calculating a precursor ion extraction ion chromatogram, XIC, of precursor ions known to correspond to the compound from precursor ion spectra acquired over the series of time periods,

calculating one or more product ions XIC of one or more product ions known to correspond to the compound from the product ion spectra acquired for each CoV over the series of time periods, an

Verifying the presence of the compound if the retention time of the XIC peak of the precursor ion XIC matches the retention time of the XIC peak of the one or more product ions XIC.

6. The system of claim 5, wherein the DMS device is further configured to separate precursor ions based on an RF separation voltage SV, the processor further receives SV for the DMS device in step (a), the processor further applies the SV to the DMS device in step (b), and the processor instructs the DMS device and tandem mass spectrometer to perform a precursor survey scan without differential mobility selection by also applying SV of zero to the DMS device.

7. The system of claim 6, wherein the SV is greater than 3000V.

8. The system of claim 6, wherein the reduced electric field E/N is greater than 100 Td.

9. The system of claim 1, wherein the mass analyzer comprises a time-of-flight TOF mass analyzer.

10. The system of claim 5, wherein the one or more compounds comprise one or more peptides.

11. A method for controlling a differential mobility spectrometry DMS apparatus and a tandem mass spectrometer to sequentially select different groups of precursor ions having different differential mobilities and mass filter, fragment and mass analyze the resulting product ions of each group, the method comprising the steps of:

(a) receiving, using a processor, a plurality of compensation voltages CoV for a DMS device and a precursor ion mass range for a mass filter of a tandem mass spectrometer, wherein the DMS device is configured to separate precursor ions based on CoV and the tandem mass spectrometer receives the separated precursor ions from the DMS device, and the tandem mass spectrometer includes the mass filter and fragmentation device for filtering and fragmenting precursor ions and a mass analyzer for mass analyzing the resulting product ions, and

(b) for each of the plurality of covs,

applying the each CoV to the DMS device to select a set of precursor ions,

instructing the mass filter to select precursor ions in the set that are within the mass range of precursor ions, thereby generating a subset of precursor ions,

instructing the fragmentation device to fragment the subset of precursor ions, thereby generating a set of product ions, an

Instructing, using the processor, the mass analyzer to measure intensities and mass-to-charge ratios, m/z, of the set of product ions, thereby generating a product ion spectrum for each of the plurality of covs.

12. The method of claim 11, wherein the DMS device is further configured to separate precursor ions based on an RF separation voltage SV, in step (a) also receiving an SV for the DMS device, and in step (b) also applying the SV to the DMS device.

13. The method of claim 11, further comprising the steps of:

instructing, using the processor, the DMS device to sample an ion beam over a series of time periods, wherein a sample separation device separates one or more compounds from a sample over time, and an ion source ionizes the separated one or more compounds and transmits ionized precursor ions as the ion beam to the DMS device, and

for each period in the series of periods,

instructing, using the processor, the DMS apparatus and tandem mass spectrometer to perform a precursor survey scan without differential mobility selection by applying a CoV of zero to the DMS apparatus, instructing the mass filter to select precursor ions in the ion beam that are within the mass range of precursor ions to produce a filtered set of precursor ions, instructing the fragmentation apparatus to transmit the filtered set of precursor ions that are not fragmented to the mass analyzer, and instructing the mass analyzer to measure intensities and m/z of the filtered set of precursor ions to produce a precursor ion spectrum, and

instructing, using the processor, the DMS device and tandem mass spectrometer to perform a sequential differential mobility-dependent product ion scan on an ion beam by performing step (b), and verifying, using the processor, the presence of a compound of the one or more compounds in the sample by:

calculating a precursor ion extraction ion chromatogram, XIC, of precursor ions known to correspond to the compound from precursor ion spectra acquired over the series of time periods,

calculating one or more product ions XIC of one or more product ions known to correspond to the compound from the product ion spectra acquired for each CoV over the series of time periods, an

Verifying the presence of the compound if the retention time of the XIC peak of the precursor ion XIC matches the retention time of the XIC peak of the one or more product ions XIC.

14. The method of claim 11, wherein the DMS device is further configured to separate precursor ions based on an RF separation voltage SV, also receive an SV for the DMS device in step (a), also apply the SV to the DMS device in step (b), and instruct the DMS device and tandem mass spectrometer to perform a precursor investigation scan without differential mobility selection by also applying an SV of zero to the DMS device.

15. A computer program product, the computer program product comprising a non-transitory and tangible computer-readable storage medium whose contents include a program with instructions being executed on a processor so as to perform a method for controlling a Differential Mobility Spectrometry (DMS) device and a tandem mass spectrometer to sequentially select different groups of precursor ions having different differential mobilities and mass filter, fragment, and mass analyze the resulting product ions of each group, the method comprising the steps of:

providing a system, wherein the system comprises one or more distinct software modules, and wherein the distinct software modules comprise a data input module and a control module;

(a) receiving, using a data input module, a plurality of compensation voltages CoV for a DMS device and a precursor ion mass range for a mass filter of a tandem mass spectrometer, wherein the DMS device is configured to separate precursor ions based on CoV and the tandem mass spectrometer receives the separated precursor ions from the DMS device, and the tandem mass spectrometer includes the mass filter and fragmentation device for filtering and fragmenting precursor ions and a mass analyzer for mass analyzing the resulting product ions, and

(b) for each of the plurality of covs,

applying the each CoV to the DMS device to select a set of precursor ions,

instructing the mass filter to select precursor ions in the set that are within the mass range of precursor ions, thereby generating a subset of precursor ions,

instructing the fragmentation device to fragment the subset of precursor ions, thereby generating a set of product ions, an

Instructing, using the control module, the mass analyzer to measure intensities and mass-to-charge ratios, m/z, of the set of product ions, thereby generating a product ion spectrum for each of the plurality of covs.

Technical Field

Introduction to

The teachings herein relate to operating a Differential Mobility Spectrometry (DMS) device and a tandem mass spectrometer to identify compounds or peptides of similar mass and with slightly different retention time behaviors. More specifically, the teachings herein relate to systems and methods for sequentially acquiring product ions of a wide mass range of precursor ions as their differential mobility is sequentially increased. The differential mobility of the precursor ions is increased step by sequentially stepping the compensation voltage (CoV) of the DMS apparatus. In each step, the same single broad mass range of precursor ions is selected and fragmented by a tandem mass spectrometer, producing a product ion spectrum.

In conventional sequential window acquisition tandem mass spectrometry (swing-MS), each product ion spectrum is acquired sequentially as the precursor ion mass selection window increases sequentially across the precursor ion mass range. Because each product ion spectrum is acquired sequentially as the differential mobility increases sequentially in the systems and methods described herein, this novel acquisition may be referred to as a DM-SWATH acquisition.

The systems and methods disclosed herein may also be performed using additional and prior sample separation devices such as, but not limited to, Liquid Chromatography (LC) devices. The systems and methods disclosed herein are also performed in conjunction with a processor, controller, microcontroller, or computer system, such as the computer system of fig. 1.

Background

Biological characterization

In conventional biological characterization, the user's goal is to obtain as much sequence coverage, (sequence linked to product ions) only via Mass Spectrometry (MS) (e.g., time-of-flight mass spectrometry (TOF-MS)) with the identified peptides verified by tandem mass spectrometry or mass spectrometry/mass spectrometry MS/MS. For MS/MS authentication, users typically rely on Information Dependent Analysis (IDA) MS/MS data collection (i.e., automatic precursor selection for MS/MS with precursor selection of 1 amu) or Sequential Window (SWATH) analysis. The percentage of validated peptides in the MS/MS mode differed between the two approaches, with the lowest IDA (typically, 50% -65%) and the highest SWATH (typically, 75% -85%). The gain supplied by SWATH relies on non-random sampling of precursor ions that can be linked to MS/MS sequence information.

Conventional SWATH can separate product ions by filtering (e.g., in a first quadrupole (Q1)) precursor ions into a wide window, thereby ensuring that the individual charge states associated with a given peptide are individually split, and that retention times (e.g., of Liquid Chromatography (LC) separation) play a key role in providing additional separation. Thus, peptides of similar quality and with little difference in retained behavior (co-elution) may not be discernable. As a result, additional systems and methods are needed to identify peptides of similar quality and with little difference in retention time behavior.

Background on Mass Spectrometry techniques

Mass spectrometers are often used in conjunction with chromatography or other separation systems in order to identify and characterize the eluted known compounds of interest from a sample. In such coupled systems, the elution solvent is ionized and a series of mass spectra are obtained from the elution solvent at specified time intervals called retention times. These retention times range from, for example, 1 second to 100 minutes or more. A series of mass spectrometric formation chromatographs or extracted ion chromatographs (XICs).

The peaks found in XIC are used to identify or characterize known peptides or compounds in a sample. More specifically, the retention time of the peak and/or the area of the peak is used to identify or characterize (quantify) the known peptide or compound in the sample.

In conventional separation-coupled mass spectrometry systems, fragment or product ions of known compounds are selected for analysis. Tandem mass spectrometry or mass spectrometry/mass spectrometry (MS/MS) scans are then performed at each interval of separation for a mass range that includes the product ion. The intensities of the product ions found in each MS/MS scan are collected over time and analyzed, for example, as a set of spectra or XICs.

In general, tandem mass spectrometry or MS/MS is a well-known technique for analyzing compounds. Tandem mass spectrometry involves ionizing one or more compounds from a sample, selecting one or more precursor ions of the one or more compounds, fragmenting the one or more precursor ions into fragments or product ions, and mass analyzing the product ions.

Tandem mass spectrometry can provide both qualitative and quantitative information. The product ion spectrum may be used to identify molecules of interest. The intensity of one or more product ions can be used to quantify the amount of compound present in the sample.

A number of different types of experimental methods or workflows can be performed using tandem mass spectrometry. Three major classes of these workflows are targeted acquisition, Information Dependent Acquisition (IDA) or Data Dependent Acquisition (DDA), and Data Independent Acquisition (DIA).

In a targeted acquisition method, one or more transitions of precursor ions to product ions are predefined for a compound of interest. The one or more transitions are interrogated or monitored during each of a plurality of time periods or cycles when the sample is introduced into the tandem mass spectrometer. In other words, the mass spectrometer selects and fragments each transformed precursor ion and performs mass analysis only for the transformed product ions. As a result, an intensity (product ion intensity) is generated for each transition. Targeted acquisition methods include, but are not limited to, multi-reaction monitoring (MRM) and Selected Reaction Monitoring (SRM).

In the IDA method, a user may specify criteria for performing non-targeted mass analysis of product ions while a sample is introduced into a tandem mass spectrometer. For example, in the IDA method, a precursor ion or Mass Spectrometry (MS) survey scan is performed to generate a precursor ion peak list. The user may select criteria to filter the peak list to obtain a subset of precursor ions on the peak list. MS/MS is then performed on each precursor ion in the subset of precursor ions. A product ion spectrum is generated for each precursor ion. The MS/MS is repeatedly performed on precursor ions in the subset of precursor ions as the sample is introduced into the tandem mass spectrometer.

However, in proteomics and many other sample types, the complexity and dynamic range of the compounds is very large. This presents challenges to traditional targeted and IDA methods, requiring very high speed MS/MS acquisition to deeply interrogate the sample in order to both identify and quantify a wide range of analytes.

As a result, the third major DIA-like method of tandem mass spectrometry was developed. These DIA methods have been used to improve the reproducibility and comprehensiveness of data collection from complex samples. The DIA method may also be referred to as a non-specific fragmentation method. In the conventional DIA method, the action of the tandem mass spectrometer is not different in MS/MS scans based on data acquired in previous precursor or product ion scans. Alternatively, a precursor ion mass range is selected. The precursor ion mass selection window is then stepped across the precursor ion mass range. All precursor ions in the precursor ion mass selection window are fragmented and all product ions of all precursor ions in the precursor ion mass selection window are mass analyzed.

The precursor ion mass selection window for the scan mass range may be very narrow, so that the probability of multiple precursors within the window is small. This type of DIA method is called, for example, MS/MS^ALL. In MS/MS^ALLIn the method, a precursor ion mass selection window of about 1amu is scanned or stepped across the entire mass range. Product ion spectra were generated for each 1amu precursor mass window. The time it takes to analyze or scan the entire mass range once is referred to as a scan cycle. However, for certain instruments and experiments, it is impractical to scan a narrow precursor ion mass selection window across a wide precursor ion mass range during each cycle.

As a result, across the entire frontThe mass range of the drive is stepped through a larger precursor ion mass selection window or a selection window with a larger width. This type of DIA method is referred to as, for example, handover acquisition. In a SWATH acquisition, the width of the precursor ion mass selection window that steps across the precursor mass range in each cycle may be 5 to 25amu or even greater. Like MS/MS^ALLAs such, all precursor ions in each precursor ion mass selection window are fragmented and all product ions of all precursor ions in each mass selection window are mass analyzed.

U.S. patent No.8,809,770 describes how SWATH acquisition can be used to provide qualitative and quantitative information about the precursor ions of a compound of interest. In particular, product ions found by splitting the precursor ion mass selection window are compared to a database of known product ions for the compound of interest. In addition, the ion trace (trace) or XIC of the product ions found by splitting the precursor ion mass selection window is analyzed to provide quantitative and qualitative information.

Fig. 2 is an example plot 200 of a precursor ion mass-to-charge ratio (m/z) range divided into ten precursor ion mass selection windows for a Data Independent Acquisition (DIA) SWATH workflow. The m/z range shown in FIG. 2 is 200 m/z. Note that the terms "mass" and "m/z" are used interchangeably herein. Typically, mass spectrometry measurements are made in m/z and then converted to mass by multiplying by charge.

Each of the ten precursor ion mass selection or isolation windows spans or has a width of 20 m/z. Three of the ten precursor ion mass selection windows, windows 201, 202, and 210, are shown in fig. 2. The precursor ion mass selection windows 201, 202, and 210 are shown as non-overlapping windows of the same width. The precursor ion mass selection window may also overlap and/or may have a variable width.

Fig. 2 depicts an immutable and non-overlapping precursor ion mass selection window used in a single cycle of an exemplary SWATH acquisition. A tandem mass spectrometer that can perform the SWATH acquisition method can also be coupled to a sample introduction device that, for example, separates one or more compounds from a sample over time. The sample introduction device may introduce the sample to the tandem mass spectrometer using techniques including, but not limited to, injection, liquid chromatography, gas chromatography, or capillary electrophoresis. The separated one or more compounds are ionized by an ion source, thereby producing an ion beam of precursor ions of the one or more compounds that are selected and fragmented by the tandem mass spectrometer.

As a result, for each time step of sample introduction of a separate compound, each of the ten precursor ion mass selection windows is selected and then split, producing ten product ion spectra for the entire m/z range. In other words, each of the ten precursor ion mass selection windows is selected and then split during each of the plurality of cycles.

Fig. 3 is an exemplary diagram 300 that graphically depicts steps for obtaining a product ion trace or XIC from each precursor ion mass selection window during each cycle of the DIA workflow. For example, ten precursor ion mass selection windows, represented in fig. 3 by precursor ion mass selection windows 201, 202, and 210, are selected and split during each of a total of 1000 cycles.

During each cycle, a product ion spectrum is obtained for each precursor ion mass selection window. For example, product ion spectrum 311 is obtained by splitting precursor ion mass selection window 201 during cycle 1, product ion spectrum 312 is obtained by splitting precursor ion mass selection window 201 during cycle 2, and product ion spectrum 313 is obtained by splitting precursor ion mass selection window 201 during cycle 1000.

By plotting the intensity of the product ions in each product ion spectrum for each precursor ion mass selection window over time, an XIC may be calculated for each product ion generated from each precursor ion mass selection window. For example, plot 320 includes the XICs calculated for each product ion of the 1000 product ion spectra of precursor ion mass selection window 201. Note that XICs may be drawn in terms of time or cycles.

The XIC in plot 320 is plotted two-dimensionally in fig. 3. However, each XIC is actually three-dimensional, as different XICs are calculated for different m/z values.

Fig. 4 is an example diagram 400 showing, in three dimensions, product ions XIC obtained over time for a precursor ion mass selection window. In FIG. 4, the x-axis is time or cycle number, the y-axis is product ion intensity, and the z-axis is m/z. From this three-dimensional graph, more information is obtained. For example, XIC peaks 410 and 420 both have the same shape and occur at the same time or at the same retention time. However, XIC peaks 410 and 420 have different m/z values. This may mean that XIC peaks 410 and 420 are isotopic peaks, or represent different product ions from the same precursor ion. Similarly, XIC peaks 430 and 440 have the same m/z value, but occur at different times. This may mean that XIC peaks 430 and 440 are the same product ion, but they are from two different precursor ions.

Figures 2 to 4 show how mass and retention time can be used to characterize compounds such as peptides using SWATH. However, as noted above, additional systems and methods are needed to identify compounds or peptides that are of similar quality and have little difference in retention time behavior.

Background on Differential Mobility Spectrometry (DMS)

Fig. 5 is a schematic diagram 500 of an exemplary DMS device. The DMS device 500 includes two parallel flat plates, plate 510 and plate 520. A Radio Frequency (RF) voltage source 530 applies an RF Separation Voltage (SV) across the plates 510 and 520, and a Direct Current (DC) voltage source 540 applies a DC offset voltage (CoV) across the plates 510 and 520. The ions 550 enter the DMS device 500 at the opening 560 as a transport gas. The separation of the ions 550 in the DMS device 500 is based on the difference in their mobility rates at high electric fields versus low electric fields.

Unlike conventional ion mobility, the ions 550 are not separated in time as they traverse the device. Alternatively, ions 550 are separated by trajectory based on the difference in their mobility between the high-field portion and the low-field portion of the applied RF voltage source 530. A high field is applied between plate 510 and plate 520 for a short period of time and then a low field is applied in the opposite direction for a longer period of time. Any difference between the low field mobility and the high field mobility of the ions of the compound of interest will cause them to migrate towards one of the plates. The ions are rotated back towards the centerline of the device by applying a second voltage offset, referred to as the CoV of the DC voltage source 540, which is a compound-specific parameter that can be used to selectively filter out all other ions. The fast switching of CoV allows the user to monitor many different compounds simultaneously. Ions 570 selected by the combination of SV and CoV exit the DMS device 500 through the opening 580 to the rest of the mass spectrometer (not shown). For example, the DMS apparatus 500 is located between the ion source (not shown) and the rest of the mass spectrometer.

Generally, the DMS device 500 has two modes of operation. In the first mode, the DMS device 500 is turned on, SV and CoV voltages are applied, and ions are separated. This is, for example, the active mode.

In a second mode of operation, the DMS device 500 is turned off, SV is set to zero, and ions 550 are simply transported from aperture 560 to aperture 580. This is, for example, a disabled or transparent mode of the DMS device 500.

In the enabled mode, the DMS device 500 may acquire data for a single MRM transition in 25 milliseconds (ms), including, for example, an inter-scan dwell time of 20 ms. In the transparent mode, the delay through the DMS device 500 is negligible.

Background on DMS acquisition

In the targeted acquisition method, the IDA method and the DIA method, Differential Mobility Spectrometry (DMS) has been used to identify compounds or peptides of similar quality and with little difference in retention time behavior.

MRM is a targeted acquisition method. U.S. patent No.9,733,214 ("the' 214 patent") relates to a method of predicting DMS device parameters for a compound when conducting MRM experiments. In developing MRM experiments, a wide range of DMS device parameter values must be interrogated for each compound or analyte, thereby reducing the number of compounds or analytes that can be tested per injection. To address this problem, the' 214 patent employs a unique MRM-triggered MRM approach. MRM triggered MRM is described, for example, in U.S. patent No.8,026,479. In the' 214 patent, the original or primary MRM transition is set with a wide step size in the DMS device parameters, such as the CoV voltage step size, and the secondary MRM transition is triggered with a finer step size in the DMS parameters.

FIG. 6 is an exemplary diagram 600 illustrating the MRM triggered MRM method of stepping DMS device parameters as described in the' 214 patent. For example, in this method, the primary precursor ion-to-product ion transition 611 is interrogated over multiple cycles of a Liquid Chromatography (LC) experiment. For the primary transition 611, the DMS device is given CoV₀. The intensity of the product ion of primary transition 611 is monitored to determine if it exceeds threshold T. As shown in plot 600, in cycle 3, the intensity of the product ion of primary transition 611 exceeds threshold T.

As a result, a series of secondary MRM transitions are triggered. For each of these secondary MRM transitions, the CoV parameters of the DMS device are increased in small steps in order to determine the optimal CoV parameters of the DMS device for that transition. For example, for the secondary transition 621, the CoV parameter of the DMS device is set to CoV₁. Similarly, for the secondary transition 622, the CoV parameter of the DMS device is set to CoV₂. This stepping of the CoV parameter of the DMS device can continue as many times as the CoV parameter of the DMS device can be changed within one cycle of the LC separation.

Note that primary transition 611 and secondary transitions 621 and 622 all represent the same precursor ion to the same product ion transition. The only difference between these transitions is the CoV parameters of the DMS device.

U.S. patent application No.15/516,387 ("the' 387 application") is directed to improving both the IDA and DIA methods by performing a precursor ion survey scan in which DMS apparatus parameters vary over time. For example, at multiple CoV intervals, a mass analyzer is used to measure the m/z intensity of precursor ions transmitted by the DMS apparatus across an m/z range. The result is a snapshot of the distribution of precursor ions in the CoV space. Assuming that there is a relationship between the m/z of the precursor ions and their optimal CoV for DMS transmission, the workflow can be automatically optimized to include precursor ions from a subset of the m/z values present at discrete CoV values.

Fig. 7 is an exemplary diagram 700 illustrating a precursor ion survey scan in which DMS apparatus parameters vary over time, as described in the' 387 application. Over time, the CoV parameters of the DMS device are derived from the CoV₁Become CoV_n. At each CoV parameter value, the same broad precursor ion mass range is selected and analyzed. Note that at each CoV parameter value, a mass analysis is performed on the precursor ion mass range, so no precursor ions are fragmented. The result is a series of precursor ion mass spectra 710 to 71 n.

The' 387 application then collected precursor ion mass spectra 710 through 71n into a thermal map that depicts precursor ion intensity as a function of mass-to-charge ratio (m/z) and CoV. Heatmaps are used to determine m/z subranges and CoV values for IDA and DIA method analysis. Full tandem mass spectrometry was then performed on these m/z subranges using IDA and DIA method analysis.

Although the methods of the '214 patent and the' 387 application have used the discriminatory power of DMS for process improvement of targeted acquisition methods, IDA acquisition methods and DIA acquisition methods, additional systems and methods are needed to specifically and directly discriminate compounds or peptides of similar quality and with little difference in retention time behavior. In other words, the techniques described in the '214 patent and the' 387 application do not achieve broad coverage of CoV values for detecting peptides on the LC time scale.

Disclosure of Invention

Systems, methods, and computer program products are disclosed for controlling a Differential Mobility Spectrometry (DMS) device and a tandem mass spectrometer to sequentially select different groups of precursor ions having different differential mobilities and to mass filter, fragment, and mass analyze the resulting product ions of each group. All three embodiments include the following steps.

A processor receives a plurality of compensation voltages (CoV) for the DMS apparatus, and a precursor ion mass range for a mass filter of a tandem mass spectrometer is received. The DMS apparatus is configured to separate precursor ions based on the CoV. The tandem mass spectrometer receives the separated precursor ions from the DMS device, and includes a mass filter and fragmentation device for filtering and fragmenting the precursor ions and a mass analyzer for mass analyzing the resulting product ions.

The processor performs a repetitive series of steps for each of the plurality of covs. CoV is applied to the DMS apparatus to select a set of precursor ions. The mass filter is instructed to select precursor ions in the set that are within a mass range of the precursor ions to generate a subset of the precursor ions. A fragmentation device is instructed to fragment a subset of the precursor ions to produce a set of product ions. The mass analyzer is instructed to measure the intensities and m/z of the set of product ions to produce a product ion spectrum for each of the plurality of covs.

In addition, the steps of the three examples were used to verify whether a known compound was in the sample. The sample separation device separates one or more compounds from the sample over time. The ion source ionizes the separated one or more compounds and transmits the ionized precursor ions as an ion beam to the DMS apparatus.

The processor instructs the DMS device to sample the ion beam over a series of time periods. For each cycle in the series, the processor performs a number of steps. In a first step, the processor instructs the DMS apparatus and the tandem mass spectrometer to perform a precursor survey scan without differential mobility selection, thereby generating a precursor ion spectrum. In a second step, the processor instructs the DMS device and the tandem mass spectrometer to perform a sequential differential mobility-dependent product ion scan on the ion beam for each of the plurality of covs, as described above.

The processor verifies the presence of a compound in the one or more compounds in the sample using the precursor ion spectrum and the product ion spectrum measured over the series of time periods. Specifically, the processor calculates a precursor ion extraction ion chromatogram (XIC) of precursor ions known to correspond to the compound from precursor ion spectra acquired over the series of time periods. The processor calculates one or more product ions XIC of the one or more product ions known to correspond to the compound from the product ion spectra acquired for each CoV over the series of time periods. The processor verifies the presence of the compound if the retention time of the XIC peak of the precursor ion XIC matches the retention time of the XIC peak of one or more product ions XIC.

These and other features of the applicants' teachings are set forth herein.

Drawings

Those skilled in the art will appreciate that the drawings described below are for illustration purposes only. The drawings are not intended to limit the scope of the present teachings in any way.

FIG. 1 is a block diagram illustrating a computer system upon which embodiments of the present teachings may be implemented.

Fig. 2 is an example plot of a precursor ion mass-to-charge ratio (m/z) range divided into ten precursor ion mass selection windows for a Data Independent Acquisition (DIA) SWATH workflow.

Figure 3 is an exemplary diagram graphically depicting steps for obtaining a product ion trace or extracting an ion chromatogram (XIC) from each precursor ion mass selection window during each cycle of the DIA workflow.

Fig. 4 is an exemplary diagram showing three dimensions of product ion XICs obtained over time for a precursor ion mass selection window.

Fig. 5 is a schematic diagram of an exemplary Differential Mobility Spectrometry (DMS) device.

FIG. 6 is an exemplary diagram of a MRM triggered MRM method showing stepping DMS device parameters as described in the' 214 patent.

Fig. 7 is an exemplary diagram illustrating a precursor ion survey scan in which DMS apparatus parameters vary over time, as described in the' 387 application.

Fig. 8 is an exemplary flow chart showing the steps of a typical acquisition during each retention time period of a conventional swing LC-MS/MS experiment designed to validate the identified peptides.

Fig. 9 is an exemplary flowchart illustrating steps designed to validate exemplary acquisition during each retention time period of a Differential Mobility (DM) -swap LC-MS/MS experiment for an identified peptide, in accordance with various embodiments.

Figure 10 is a schematic diagram of a system for controlling a DMS apparatus and a tandem mass spectrometer to sequentially select different groups of precursor ions having different differential mobilities and mass filter, fragment, and mass analyze the resulting product ions of each group, in accordance with various embodiments.

Fig. 11 is an example diagram illustrating how the DMS apparatus of fig. 10 separates groups of precursor ions having different differential mobilities and how the tandem mass spectrometer of fig. 10 mass filters, splits, and mass analyzes the resulting product ions of each group, in accordance with various embodiments.

Figure 12 is a flow diagram illustrating a method for controlling a DMS apparatus and a tandem mass spectrometer to sequentially select different groups of precursor ions having different differential mobilities and mass filter, fragment, and mass analyze the resulting product ions of each group, in accordance with various embodiments.

Fig. 13 is a schematic diagram of a system including one or more different software modules that execute a method for controlling a DMS apparatus and a tandem mass spectrometer to sequentially select different groups of precursor ions having different differential mobilities and mass filter, fragment, and mass analyze the resulting product ions of each group, in accordance with various embodiments.

Before one or more embodiments of the present teachings are described in detail, those skilled in the art will understand that the present teachings are not limited in their application to the details of construction, the arrangement of components, and the arrangement of steps set forth in the following detailed description or illustrated in the drawings. Also, it is to be understood that the phraseology and terminology used herein is for the purpose of description and should not be regarded as limiting.

Detailed Description

Computer-implemented system

FIG. 1 is a block diagram that illustrates a computer system 100 upon which an embodiment of the present teachings may be implemented. Computer system 100 includes a bus 102 or other communication mechanism for communicating information, and a processor 104 coupled with bus 102 for processing information. Computer system 100 also includes a memory 106 coupled to bus 102 for storing instructions to be executed by processor 104, memory 106 may be a Random Access Memory (RAM) or other dynamic storage device. Memory 106 may also be used for storing temporary variables or other intermediate information during execution of instructions to be executed by processor 104. Computer system 100 further includes a Read Only Memory (ROM)108 or other static storage device coupled to bus 102 for storing static information and instructions for processor 104. A storage device 110, such as a magnetic disk or optical disk, is provided and coupled to bus 102 for storing information and instructions.

Computer system 100 may be coupled via bus 102 to a display 112, such as a Cathode Ray Tube (CRT) or Liquid Crystal Display (LCD), for displaying information to a computer user. An input device 114, including alphanumeric and other keys, is coupled to bus 102 for communicating information and command selections to processor 104. Another type of user input device is cursor control 116, such as a mouse, a trackball, or cursor direction keys for communicating direction information and command selections to processor 104 and for controlling cursor movement on display 112. The input device typically has two degrees of freedom in two axes, a first axis (i.e., x) and a second axis (i.e., y), that allows the device to specify positions in a plane.

Computer system 100 may perform the present teachings. Consistent with certain implementations of the present teachings, the results are provided by computer system 100 in response to processor 104 executing one or more sequences of one or more instructions contained in memory 106. Such instructions may be read into memory 106 from another computer-readable medium, such as storage device 110. Execution of the sequences of instructions contained in memory 106 causes processor 104 to perform the processes described herein. Alternatively, hardwired circuitry may be used in place of or in combination with software instructions to implement the teachings. Thus, implementations of the present teachings are not limited to any specific combination of hardware circuitry and software.

In various embodiments, computer system 100 may be connected across a network to one or more other computer systems, such as computer system 100, to form a networked system. The network may comprise a private network or a public network such as the internet. In a networked system, one or more computer systems may store data and supply the data to other computer systems. In a cloud computing scenario, one or more computer systems that store and provision data may be referred to as a server or a cloud. For example, one or more computer systems may include one or more network servers. For example, other computer systems that send and receive data to and from a server or cloud may be referred to as clients or cloud devices.

The term "computer-readable medium" as used herein refers to any medium that participates in providing instructions to processor 104 for execution. Such a medium may take many forms, including but not limited to, non-volatile media, and transmission media. Non-volatile media includes, for example, optical or magnetic disks, such as storage device 110. Volatile media includes dynamic memory, such as memory 106. Transmission media includes coaxial cables, copper wire and fiber optics, including the wires that comprise bus 102.

Common forms of computer-readable media or computer program products include, for example, a floppy disk, a flexible disk, hard disk, magnetic tape, or any other magnetic medium, a CD-ROM, Digital Video Disk (DVD), blu-ray disk, any other optical medium, thumb drives, memory cards, a RAM, a PROM, and EPROM, a flash EPROM, any other memory chip or cartridge, or any other tangible medium from which a computer can read.

Various forms of computer readable media may be involved in carrying one or more sequences of one or more instructions to processor 104 for execution. For example, the instructions may initially be carried on a magnetic disk of a remote computer. The remote computer can load the instructions into its dynamic memory and send the instructions over a telephone line using a modem. A modem local to computer system 100 can receive the data on the telephone line and use an infra-red transmitter to convert the data to an infra-red signal. An infrared detector coupled to bus 102 can receive the data carried in the infrared signal and place the data on bus 102. The bus 102 carries the data to the memory 106, and the processor 104 retrieves instructions from the memory 106 and executes the instructions. Alternatively, the instructions received by memory 106 may be stored on storage device 110 either before or after execution by processor 104.

According to various embodiments, instructions configured to be executed by a processor to perform a method are stored on a computer-readable medium. The computer readable medium may be a device that stores digital information. For example, the computer readable medium includes a compact disk read only memory (CD-ROM) for storing software as is known in the art. The computer readable medium is accessed by a processor adapted to execute instructions configured to be executed.

The following description of various implementations of the present teachings has been presented for purposes of illustration and description. The description is not intended to be exhaustive or to limit the present teachings to the precise form disclosed. Modifications and variations are possible in light of the above teachings or may be acquired from practice of the present teachings. Additionally, the described implementations include software, but the present teachings may be implemented as a combination of hardware and software or as hardware alone. The present teachings can be implemented with both object-oriented and non-object-oriented programming systems.

DM-SWATH system and method

As described above, in conventional biological characterization, the user's goal is to obtain as much sequence coverage, (sequences linked to fragment ions) only via Mass Spectrometry (MS) (e.g., time of flight mass spectrometry (TOF-MS)) with the identified peptides verified by tandem mass spectrometry or mass spectrometry/mass spectrometry MS/MS. Conventional sequential window acquisition tandem mass spectrometry (SWATH-MS) can separate product ions by filtering (e.g., in a first quadrupole (Q1)) precursor ions into a wide window, ensuring that the individual charge states associated with a given peptide are individually split, and that retention times (e.g., of Liquid Chromatography (LC) separation) play a key role in providing additional separation. Thus, peptides of similar quality and with little difference in retained behavior (co-elution) may not be discernable. As a result, additional systems and methods are needed to identify peptides of similar quality and with little difference in retention time behavior.

In various embodiments, the systems and methods involve operating a Differential Mobility Spectrometry (DMS) device and a tandem mass spectrometer to discriminate between compounds or peptides that are of similar mass and have slightly different retention time behaviors. More particularly, the systems and methods relate to sequentially acquiring product ions of precursor ions of a wide mass range as the differential mobility of the precursor ions sequentially increases.

In conventional SWATH-MS, each product ion spectrum is acquired sequentially as the precursor ion mass selection window sequentially increases across the precursor ion mass range. Because each product ion spectrum is acquired sequentially as the order of Differential Mobility (DM) increases in the systems and methods described herein, this novel acquisition may be referred to as a DM-SWATH acquisition.

In DM-SWATH acquisition, orthogonal separation of peptides is provided by adding a differential mobility dimension (DMs gas phase separation) of peptide ions prior to precursor selection. In this approach, the conventional SWATH dimension (single wide Q1 window, m/z 300- "1200") is relaxed, but the DMS dimension is added, which is orthogonal to both LC and MS. The DMS dimension is applied to capture MS/MS information acquired at a series of CoV values (5 to 20 with step size adjusted to instrument resolution) at elevated separation voltages (SV >3000V, representing a reduced electric field (E/N) of-100 townsend (td)). For example, MS information is acquired with a DMS device set to a transmission mode (SV ═ CoV ═ 0V). The overall duty cycle of the DMS dimension is faster than the SWATH dimension of conventional SWATH-MS, while providing orthogonality with the data (separation of equal-gravity peptides).

Fig. 8 is an exemplary flowchart 800 showing the steps of a typical acquisition during each retention time period of a conventional SWATH LC-MS/MS experiment designed to validate identified peptides.

In step 810, low Collision Energy (CE) time of flight (TOF) Mass Spectrometry (MS) analysis is performed. Essentially, precursor ion mass analysis is performed for a wide precursor ion mass range of 350 to 1200 m/z. A precursor ion mass spectrum is generated by mass analysis of the precursor ions. From all precursor ion mass spectra generated over multiple cycles of a Liquid Chromatography (LC) experiment, an extracted ion chromatogram (XIC) was calculated for each precursor ion found.

In step 820, TOF mass spectrometry/mass spectrometry (MS/MS) analysis is performed with a CE of 16eV for a narrow precursor mass range of 350 to 400 m/z. Essentially, precursor ions in a narrow 50m/z precursor mass selection window from 350 to 400m/z are selected and fragmented. The resulting product ions were then mass analyzed. A product ion mass spectrum is generated by mass analysis of the product ions.

In step 830, the 50m/z precursor mass selection window is moved or stepped substantially to a precursor ion mass range from 400 to 450 m/z. Also, precursor ions in the mass range of the precursor ions are selected and fragmented, and the product ions are mass analyzed and a product ion mass spectrum is generated. For the split in this step, the CE was increased to 18 eV.

In step 840, the 50m/z precursor mass selection window is again moved or stepped to a precursor ion mass range from 450 to 500 m/z. Likewise, precursor ions in the mass range of the precursor ions are again selected and fragmented, and the product ions are mass analyzed and a product ion mass spectrum is generated. For the cleavage in this step, the CE was increased to 21 eV.

The narrower 50m/z precursor mass selection window is stepped across the wide precursor mass range of 350 to 1200m/z and the process of adding CE continues for an additional 14 steps. The total number of steps was 17. The last two steps are shown as steps 850 and 860.

From each of the 17 steps of each cycle, a product ion spectrum was obtained. As a result, 17 product ion spectra were obtained for each cycle. XICs are calculated for each product ion found from all product ion mass spectra generated for each narrower precursor ion mass range over multiple cycles of the LC experiment.

For example, a peptide represented by a precursor ion is validated by finding its corresponding product ion. The corresponding product ion for the narrower 50m/z mass range of the precursor ion is found by matching the retention time of its XIC peak to that of the precursor ion.

Fig. 9 is an exemplary flowchart 900 illustrating steps of an exemplary acquisition during each retention time period of a DM-handover LC-MS/MS experiment designed to validate identified peptides, in accordance with various embodiments.

In step 910, low Collision Energy (CE) time of flight (TOF) Mass Spectrometry (MS) analysis is performed, as in conventional SWATH LC-MS/MS. The DMS device is turned off by setting the compensation voltage (CoV) and the RF Split Voltage (SV) to zero. Precursor ion mass analysis is performed for a wide precursor ion mass range of 350 to 1200 m/z. From the precursor ion mass analysis, a precursor ion mass spectrum is generated. XICs are calculated for each precursor ion found from all precursor ion mass spectra generated over multiple cycles of a Liquid Chromatography (LC) experiment.

In step 920, the DMS device is turned on using CoV of 5V and SV of 3000V (or E/N100 Td). For precursor ions selected by the DMS apparatus, TOF-MS/MS analysis is performed for the entire broad precursor ion mass range of 350 to 1200 m/z. Thus, precursor ions selected by the DMS apparatus and in a wide precursor ion mass range of 350 to 1200m/z are selected and fragmented. The resulting product ions were then mass analyzed. From this product ion mass analysis, a product ion mass spectrum is generated.

In step 930, the CoV of the DMS device is essentially stepped to a higher value of 6V. The SV of the DMS device is kept constant at 3000V (or E/N-100 Td). Also, for precursor ions selected by the DMS apparatus at the CoV, precursor ions are further selected and fragmented from a wide precursor ion mass range of 350 to 1200m/z, the resulting product ions are mass analyzed, and a product ion mass spectrum is generated.

In step 940, the CoV of the DMS device is stepped or increased again to a higher value of 7V. The SV of the DMS device is kept constant at 3000V (or E/N100 Td). In addition, also for precursor ions selected by the DMS apparatus at the CoV, precursor ions are further selected and fragmented from a broad precursor ion mass range of 350 to 1200m/z, the resulting product ions are mass analyzed, and a product ion mass spectrum is generated.

The process of stepping the CoV value of the DMS device continues for another 8 steps. The total number of steps is 11. The last two steps are shown as steps 950 and 960. The SV value of the DMS apparatus, the precursor ion mass selection window of the tandem mass spectrometer and the CE of the tandem mass spectrometer were all kept constant for all 11 steps.

From each of the 11 steps in each cycle, a product ion spectrum was obtained. As a result, 11 product ion spectra were obtained for each cycle. XICs are calculated for each product ion found from all product ion mass spectra generated for each CoV value of the DMS device over multiple cycles of the LC experiment.

In fig. 9, the CoV of the DMS device starts at a low value and is stepped up or increased to a higher value in each step. In an alternative embodiment, the CoV of the DMS device starts at a high value and is stepped or reduced to a lower value in each step. In a further alternative embodiment, the predetermined list CoV values of the DMS device may have different intensity levels. For example, the CoV value on the list may be increased and decreased multiple times. In this case, the CoV of the DMS device starts at an initial value and is stepped or moved to the next value in the list in each step. In other words, the CoV values on the list do not necessarily have to increase or decrease uniformly, but may vary randomly. Although the sequential variation of the CoV values can be varied randomly, the values used must be known and repeatable.

As in conventional SWATH LC-MS/MS, the peptide represented by the precursor ion is validated, for example, by finding its corresponding product ion. The corresponding product ion for the narrower 50m/z mass range of the precursor ion is found by matching the retention time of its XIC peak to that of the precursor ion.

DM-SWATH system

Fig. 10 is a schematic diagram of a system 1000 for controlling a DMS apparatus and a tandem mass spectrometer to sequentially select different groups of precursor ions having different differential mobilities and mass filter, fragment, and mass analyze the resulting product ions of each group, in accordance with various embodiments. System 1000 includes DMS device 1010, tandem mass spectrometer 1020, and processor 1030.

The DMS apparatus 1010 is configured to separate precursor ions based on a compensation voltage (CoV). An exemplary DMS device is SelexION, produced by SCIEX^TMAn apparatus.

Tandem mass spectrometer 1020 receives the separated precursor ions from DMS apparatus 1010. Tandem mass spectrometer 1020 includes a mass filter 1021 and a fragmentation device 1022 for filtering and fragmenting precursor ions and a mass analyzer 1023 for mass analyzing the resulting product ions. The mass filter 1021 is shown as a quadrupole. However, the mass filter 1021 may be any type of mass filter. The splitting device 1022 is shown as a quadrupole. However, the splitting apparatus 1022 may be any type of splitting apparatus. The mass analyzer 1023 is shown as a time-of-flight (TOF) mass analyzer. However, the mass analyzer 1023 can be any type of mass analyzer. The mass analyzer of the tandem mass spectrometer may include, but is not limited to, for example, a time-of-flight (TOF) device, a quadrupole, an ion trap, a linear ion trap, an orbitrap, a magnetic quad-sector mass analyzer, a hybrid quadrupole time-of-flight (Q-TOF) mass analyzer, or a fourier transform mass analyzer.

Processor 1030 communicates with DMS device 1010 and tandem mass spectrometer 1020. Processor 1030 may be, but is not limited to, the system of figure 1, a computer, a microprocessor, a microcontroller, or any device capable of sending control signals and data to and receiving control signals and data from DMS device 1010 and tandem mass spectrometer 1020 and other devices.

The processor 1030 receives a plurality of compensation voltages (CoV) for the DMS apparatus 1010 and a precursor ion mass range for the mass filter 1021. The plurality of CoV and precursor ion mass ranges may be received from a user through a user interface (not shown) or received from a memory (not shown). The plurality of CoV and precursor ion mass ranges may be defined as part of a standard acquisition method or as part of a custom experiment.

In a preferred embodiment, the plurality of covs is a plurality of incremental covs. In an alternative embodiment, the plurality of covs is a plurality of decreasing covs. In another alternative embodiment, the plurality of covs is a plurality of randomly varying covs.

Processor 1030 performs a plurality of steps for each of the plurality of covs. In a first step, the processor 1030 applies CoV to the DMS apparatus 1010 to select a set of precursor ions. For example, the processor 1030 applies CoV to the DMS device 1010 by controlling the CoV voltage source 1011.

Figure 11 is an example diagram 1100 illustrating how the DMS apparatus 1010 of figure 10 separates groups of precursor ions having different differential mobilities and how the tandem mass spectrometer 1020 of figure 10 mass filters, splits, and mass analyzes the resulting product ions of each group, in accordance with various embodiments.

Graphs 1111, 1112, and 111n illustrate how the DMS device 1010 of FIG. 10 targets multiple CoVs (CoVs)₁、CoV₂And CoV_n) Each of which selects a set of precursor ions. For example, for CoV₁Selection of precursor ions 1121 for CoV₂Selecting precursor ions 1122, and for CoV_nPrecursor ions 112n are selected. Note that the selection of precursor ions 1121, 1122, and 112n is not related to m/z. It is also noted that plots 1111, 1112, and 111n are for illustrative purposes only, and that the set of precursor ions selected by DMS apparatus 1010 of fig. 10 is not mass analyzed, and is not selected using a mass window.

Returning to fig. 10, processor 1030 instructs mass filter 1021 to select precursor ions in the set that are within the mass range of the precursor ions, thereby generating a subset of precursor ions. Plots 1131, 1132, and 113n of fig. 11 illustrate how the mass filter 1021 of fig. 10 selects precursor ions in the set that are within the mass range of the precursor ions. For example, the precursor ion mass selection window 1140 selects precursor ions in the set that are within the same precursor ion mass range. Also note that plots 1131, 1132, and 113n are for illustration purposes only, and no mass analysis of precursor ions in a group that are within the same precursor ion mass range is performed.

Returning to fig. 10, processor 1030 instructs fragmentation device 1022 to fragment the subset of precursor ions, thereby generating a set of product ions. Plots 1151, 1152, and 115n of fig. 11 illustrate the subset of precursor ions that are fragmented by the fragmentation device 1022 of fig. 10. Also note that plots 1151, 1152, and 115n are for illustration purposes only, and that the fragmented subset of precursor ions is not mass analyzed.

Returning to fig. 10, processor 1030 instructs mass analyzer 1023 to measure the intensities and m/z of the set of product ions, thereby generating a product ion spectrum for each of the plurality of covs. Plots 1161, 1162, and 116n of fig. 11 illustrate product ion spectra measured by mass analyzer 1023 of fig. 10 for each of a plurality of covs.

Returning to fig. 10, processor 1030 directs mass filter 1021, splitting device 1022, and mass analyzer 1023 by applying one or more voltages to these devices, e.g., using one or more voltage sources (not shown).

For example, DMS apparatus 1010 may also be configured to separate precursor ions based on RF SVs received by processor 1030. Processor 1030 applies the same SV to the DMS device for each of the plurality of covs. For example, the processor 1030 applies SV to the DMS device 1010 by controlling the SV voltage source 1012. In various embodiments, the received SV is greater than 3000V (or E/N is greater than 100 Td).

In various embodiments, the system 1000 of fig. 10 is also used to verify whether a known compound is in the sample. The system 1000 also includes a sample separation device 1040 and an ion source 1050. The sample separation device 1040 uses techniques including, but not limited to, liquid chromatography, gas chromatography, capillary electrophoresis, or ion mobility. Ion source 1050 uses techniques including, but not limited to, electrospray ionization (ESI) (e.g., nanospray) or matrix-assisted laser desorption/ionization (MALDI).

The sample separation device 1040 separates one or more compounds from a sample over time. The ion source 1050 ionizes the separated compound or compounds and transmits the ionized precursor ions as an ion beam to the DMS apparatus 1010.

The processor 1030 directs the DMS device 1010 to sample the ion beam over a series of time periods. For each cycle in the series, processor 1030 performs a number of steps. In a first step, processor 1030 instructs DMS apparatus 1010 and tandem mass spectrometer 1020 to perform a precursor survey scan without differential mobility selection. This is performed by the following steps: a CoV of zero is applied to the DMS apparatus 1010, the mass filter 1021 is instructed to select precursor ions in the ion beam that are within a mass range of the precursor ions to produce a filtered set of precursor ions, the fragmentation apparatus 1022 is instructed to transmit the non-fragmented filtered set of precursor ions to the mass analyzer 1023, and the mass analyzer 1023 is instructed to measure the intensities and m/z of the filtered set of precursor ions to produce a precursor ion spectrum.

In a second step, processor 1030 instructs DMS device 1010 and tandem mass spectrometer 1020 to perform a sequential differential mobility-dependent product ion scan on the ion beam for each of the plurality of covs as described above.

Processor 1030 validates the presence of a compound in the one or more compounds in the sample using the precursor ion spectrum and the product ion spectrum measured over the series of time periods. Specifically, processor 1030 calculates a precursor ion XIC of a precursor ion known to correspond to the compound from precursor ion spectra acquired over a series of time periods. Processor 1030 calculates one or more product ions XIC of one or more product ions known to correspond to the compound from the product ion spectra acquired for each CoV over a series of time periods. Processor 1030 verifies the presence of the compound if the retention time of the XIC peak of precursor ion XIC matches the retention time of the XIC peak of one or more product ions XIC.

As described above, DMS apparatus 1010 may also be configured to separate precursor ions based on SVs received by processor 1030, for example. As a result, processor 1030 instructs DMS apparatus 1010 and tandem mass spectrometer 1020 to also perform a precursor survey scan without differential mobility selection by applying an SV of zero to DMS apparatus 1010.

In various embodiments, the one or more compounds can be one or more peptides.

DM-SWATH method

Figure 12 is a flow diagram illustrating a method 1200 for controlling a DMS apparatus and a tandem mass spectrometer to sequentially select different groups of precursor ions having different differential mobilities and mass filter, fragment, and mass analyze the resulting product ions of each group, in accordance with various embodiments.

In step 1210, a plurality of covs for a DMS apparatus and a precursor ion mass range for a mass filter of a tandem mass spectrometer are received using a processor. The DMS apparatus is configured to separate precursor ions based on the CoV. The tandem mass spectrometer receives the separated precursor ions from the DMS apparatus and includes a mass filter and fragmentation apparatus for filtering and fragmenting the precursor ions and a mass analyzer for mass analyzing the resulting product ions.

In step 1220, a repeated series of steps is performed for each of the plurality of covs using the processor.

In step 1230, CoV is applied to the DMS apparatus to select a set of precursor ions.

In step 1240, the mass filter is instructed to select precursor ions in the set that are within the mass range of the precursor ions, thereby generating a subset of precursor ions.

In step 1250, the fragmentation device is instructed to fragment a subset of precursor ions, thereby generating a set of product ions.

In step 1260, the processor instructs the mass analyzer to measure the intensities and m/z of the set of product ions, thereby generating a product ion spectrum for each of the plurality of covs.

DM-SWATH computer program product

In various embodiments, a computer program product includes a tangible computer readable storage medium whose contents include a program with instructions being executed on a processor to perform a method for controlling a DMS device and a tandem mass spectrometer to sequentially select different groups of precursor ions having different differential mobilities and mass filter, fragment, and mass analyze the resulting product ions of each group. The method is performed by a system comprising one or more distinct software modules.

Fig. 13 is a schematic diagram of a system 1300 including one or more different software modules that execute a method for controlling a DMS device and a tandem mass spectrometer to sequentially select different groups of precursor ions having different differential mobilities and mass filter, fragment, and mass analyze the resulting product ions of each group, in accordance with various embodiments. The system 1300 includes an input data module 1310 and a control module 1320.

The input data module 1310 receives a plurality of covs for a DMS apparatus and precursor ion mass ranges for a mass filter of a tandem mass spectrometer. The DMS apparatus is configured to separate precursor ions based on the CoV. The tandem mass spectrometer receives the separated precursor ions from the DMS apparatus and includes a mass filter and fragmentation apparatus for filtering and fragmenting the precursor ions and a mass analyzer for mass analyzing the resulting product ions.

The control module 1320 performs a repeated series of steps for each of the plurality of covs. Each CoV is applied to a DMS apparatus to select a set of precursor ions. The mass filter is instructed to select precursor ions in the set that are within a mass range of the precursor ions to produce a subset of the precursor ions. A fragmentation device is instructed to fragment a subset of the precursor ions to produce a set of product ions. The mass analyzer is instructed using the control module to measure the intensities and mass-to-charge ratios (m/z) of the set of product ions to generate a product ion spectrum for each of the plurality of covs.

While the present teachings are described in conjunction with various embodiments, it is not intended that the present teachings be limited to such embodiments. On the contrary, the present teachings encompass various alternatives, modifications, and equivalents, as will be appreciated by those skilled in the art.

In addition, in describing various embodiments, the specification may have presented the method and/or process as a particular sequence of steps. However, to the extent that the method or process does not rely on the particular order of steps set forth herein, the method or process should not be limited to the particular sequence of steps described. Other sequences of steps may be possible, as will be appreciated by those of ordinary skill in the art. Therefore, the particular order of the steps set forth in the specification should not be construed as limitations on the claims. Additionally, the claims directed to the method and/or process should not be limited to the performance of their steps in the order written, and one skilled in the art can readily appreciate that the sequences may be varied and still remain within the spirit and scope of the various embodiments.