阅读说明：本技术 一种增强免疫力的灵芝提取物 (Ganoderma lucidum extract for enhancing immunity ) 是由 赵伟 彭邦宏 于 2020-05-29 设计创作，主要内容包括：本发明公开了一种增强免疫力的灵芝提取物的提取方法,包括如下步骤：向灵芝孢子粉中加入水搅拌均匀,加入复合酶制剂,40-50℃酶解10-16h,得到酶解料；向酶解料加入菊糖、透明质酸混合均匀,加入无水乙醇至乙醇浓度为40-45wt％,80-90℃回流加热1-2h,过滤,得到第一滤液和第一滤渣；向第一滤渣中加入乙醇溶液,98-105℃回流加热20-40min,过滤,得到第二滤液；合并第一滤液、第二滤液,真空浓缩,冷冻干燥,得到增强免疫力的灵芝提取物。本发明提取效率高,营养价值大大提高,而且机体吸收利用率高,可有效调节免疫,抗氧化、抗衰老,增强基体免疫功能的作用明显提升。(The invention discloses an extraction method of a ganoderma lucidum extract for enhancing immunity, which comprises the following steps: adding water into Ganoderma spore powder, stirring, adding complex enzyme preparation, and performing enzymolysis at 40-50 deg.C for 10-16 hr to obtain enzymolysis material; adding inulin and hyaluronic acid into the enzymolysis material, mixing, adding anhydrous ethanol until ethanol concentration is 40-45 wt%, reflux-heating at 80-90 deg.C for 1-2 hr, and filtering to obtain first filtrate and first residue; adding ethanol solution into the first filter residue, heating under reflux at 98-105 deg.C for 20-40min, and filtering to obtain second filtrate; mixing the first filtrate and the second filtrate, vacuum concentrating, and freeze drying to obtain Ganoderma extract with immunity enhancing effect. The invention has high extraction efficiency, greatly improved nutritive value and high absorption and utilization rate of organism, can effectively regulate immunity, resist oxidation and aging and obviously improve the function of enhancing the immunologic function of a matrix.)

1. The extraction method of the ganoderma lucidum extract for enhancing the immunity is characterized by comprising the following steps:

s1, adding water into the ganoderma lucidum spore powder, stirring uniformly, adding the complex enzyme preparation, and performing enzymolysis for 10-16h at 40-50 ℃ to obtain an enzymolysis material;

s2, adding inulin and hyaluronic acid into the enzymolysis material, mixing uniformly, adding absolute ethyl alcohol until the concentration of the ethyl alcohol is 40-45 wt%, refluxing and heating at 80-90 ℃ for 1-2h, and filtering to obtain a first filtrate and a first filter residue; adding ethanol solution into the first filter residue, heating under reflux at 98-105 deg.C for 20-40min, and filtering to obtain second filtrate;

s3, combining the first filtrate and the second filtrate, vacuum concentrating, and freeze drying to obtain the ganoderma lucidum extract for enhancing immunity.

2. The extraction method of ganoderma lucidum extract for enhancing immunity as claimed in claim 1, wherein in S1, the mass ratio of ganoderma lucidum spore powder, water and complex enzyme preparation is 10-15: 40-60: 0.1-0.2.

3. The method for extracting ganoderma lucidum extract for enhancing immunity as claimed in claim 1, wherein in S1, the complex enzyme preparation comprises: papain, trypsin, subtilisin and lipase.

4. The method for extracting ganoderma lucidum extract for enhancing immunity as claimed in claim 3, wherein the mass ratio of papain, trypsin, subtilisin and lipase is 1-2: 1-2: 1-2: 2-4.

5. The method for extracting ganoderma lucidum extract for enhancing immunity according to claim 1, wherein in S2, the mass ratio of enzymolysis material, inulin and hyaluronic acid is 50-70: 0.5-1.5: 0.2-0.6.

6. The method for extracting ganoderma lucidum extract for enhancing immunity according to claim 1, wherein in the step of S2, 50-60 wt% ethanol solution is added to the first filter residue.

7. An immunity-enhancing ganoderma lucidum extract, which is prepared by the extraction method of the immunity-enhancing ganoderma lucidum extract as claimed in any one of claims 1 to 6.

Technical Field

The invention relates to the technical field of ganoderma lucidum extracts, and particularly relates to a ganoderma lucidum extract for enhancing immunity and an extraction method thereof.

Background

The ganoderma lucidum belongs to a class of fungal organisms distributed in the nature, is one of traditional rare Chinese medicinal materials, and can be used as both medicine and food. Both ancient and modern pharmacological and clinical studies prove that the ganoderma lucidum indeed has the effects of preventing and treating diseases and prolonging life, and has wide pharmacological effects, such as anti-tumor effect, immunoregulation effect, anti-radiation and anti-chemotherapy effect, calming effect, cardiotonic and anti-myocardial ischemia effect, blood fat regulation effect, blood sugar reduction effect, asthma relief effect, liver protection effect, anti-hypoxia effect, anti-aging effect and the like.

The active ingredients of ganoderma lucidum, which is a natural medicine, mainly exist in ganoderma lucidum sporocarp and ganoderma lucidum spore, and are fat-soluble volatile ingredients. The ganoderma lucidum contains various polysaccharides, polypeptides, amino acids, triterpenes, nucleosides and the like, and has wide market prospect. In the prior art, the extraction method for the functional components of the ganoderma lucidum is easy to cause reduction of biological activity, and in addition, the technical defects of more complicated process and higher cost exist, so the solution is urgently needed.

Disclosure of Invention

Based on the technical problems in the background art, the invention provides the lucid ganoderma extract for enhancing the immunity and the extraction method thereof, the extraction efficiency is high, the protein, the lucid ganoderma triterpene and the lucid ganoderma polysaccharide are improved by 2-4 times compared with the common method, the nutritive value is greatly improved, the absorption and utilization rate of the organism is high, the functions of effectively regulating the immunity, resisting oxidation and aging and enhancing the immune function of a matrix are obviously improved.

The invention provides an extraction method of a ganoderma lucidum extract for enhancing immunity, which comprises the following steps:

s1, adding water into the ganoderma lucidum spore powder, stirring uniformly, adding the complex enzyme preparation, and performing enzymolysis for 10-16h at 40-50 ℃ to obtain an enzymolysis material;

s2, adding inulin and hyaluronic acid into the enzymolysis material, mixing uniformly, adding absolute ethyl alcohol until the concentration of the ethyl alcohol is 40-45 wt%, refluxing and heating at 80-90 ℃ for 1-2h, and filtering to obtain a first filtrate and a first filter residue; adding ethanol solution into the first filter residue, heating under reflux at 98-105 deg.C for 20-40min, and filtering to obtain second filtrate;

s3, combining the first filtrate and the second filtrate, vacuum concentrating, and freeze drying to obtain the ganoderma lucidum extract for enhancing immunity.

Preferably, in S1, the mass ratio of the ganoderma lucidum spore powder, the water and the compound enzyme preparation is 10-15: 40-60: 0.1-0.2.

Preferably, in S1, the complex enzyme preparation comprises: papain, trypsin, subtilisin and lipase.

Preferably, the mass ratio of the papain, the trypsin, the subtilisin and the lipase is 1-2: 1-2: 1-2: 2-4.

Preferably, in S2, the mass ratio of enzymolysis material, inulin and hyaluronic acid is 50-70: 0.5-1.5: 0.2-0.6.

Preferably, in S2, 50-60 wt% ethanol solution is added to the first residue.

A Ganoderma extract for enhancing immunity is prepared by the above method.

The technical effects of the invention are as follows:

according to the invention, the ganoderma lucidum raw material is subjected to enzymolysis by the complex enzyme preparation, so that the release of active substances in ganoderma lucidum sporocarp can be promoted, the ganoderma lucidum extract is easy to separate out, the sufficiency of subsequent extraction can be effectively improved, and simultaneously, the mixing of non-functional components is avoided; the addition of the inulin can protect the enzymolysis products and ensure that the functional components are retained to the maximum extent, and the hyaluronic acid is used as the acidic mucopolysaccharide, so that the invasion of microorganisms can be prevented, the inulin can be fully dispersed on the surfaces of the enzymolysis products, the obtained products can be directly taken without further deep processing, the inulin does not participate in human metabolism and is excreted in urine in the original form after entering the human body, and the hyaluronic acid can promote the better absorption of the nutrients of the ganoderma lucidum, thereby achieving the ideal effect of promoting the nutrient absorption.

In the ganoderma lucidum, ganoderma lucidum polysaccharide is a non-specific immunopotentiator, which not only can enhance the cellular immunity, but also can enhance the humoral immunity, promote the phagocytic capacity of macrophages and directly produce the anti-tumor effect; the ganoderma lucidum triterpene is a primary metabolite and a secondary metabolite of ganoderma lucidum mycelia, and is a main functional component of ganoderma lucidum which plays roles of resisting inflammation, easing pain, calming, resisting aging, poisoning tumor cells, resisting anoxia and the like.

Through detection: in the ganoderma lucidum extract, the protein, the ganoderma lucidum triterpene and the ganoderma lucidum polysaccharide are improved by 2-4 times compared with the common method, the nutritive value is greatly improved, the ganoderma lucidum extract is convenient for human body to absorb and utilize, and the functions of effectively regulating immunity, resisting oxidation and aging and enhancing the immune function of a matrix are obviously improved.

Detailed Description

The technical solution of the present invention will be described in detail below with reference to specific examples.