阅读说明：本技术 一种适用于高尿酸血症或痛风患者的药食同源酵素及其制备方法 (Medicine-food homologous enzyme suitable for patients with hyperuricemia or gout and preparation method thereof ) 是由 唐美荣 于 2020-07-29 设计创作，主要内容包括：本发明提供了一种适用于高尿酸血症或痛风患者的药食同源酵素及其制备方法,本发明以乌鞘蛇、阿胶、桔梗、余甘子、葛根、布渣叶、荜茇、郁李仁等为原料制成酵素产品,所有原料均在卫健委公布的药食同源名单中列出,可日常食用,没有毒副作用,有效控制高尿酸血症或痛风患者血尿酸等指标水平,具有良好的保健效果。(The invention provides a medicinal and edible enzyme suitable for patients with hyperuricemia or gout and a preparation method thereof.)

1. A preparation method of a medicine-food homologous enzyme suitable for patients with hyperuricemia or gout is characterized by comprising the following specific steps in parts by weight:

(1) firstly, 100 parts of platycodon grandiflorum, 30-40 parts of emblic leafflower fruit, 20-25 parts of kudzu vine root, 10-15 parts of microcos paniculata, 8-10 parts of long pepper and 5-8 parts of bunge cherry seed are respectively crushed into powder and mixed to obtain mixed powder, and then the mixed powder is prepared by the following steps of: 3-4: 100, extracting to obtain an extracting solution, performing enzymolysis by using complex enzyme, and concentrating to obtain a concentrated solution;

(2) respectively crushing 70-80 parts of zaocys dhumnade and 5-8 parts of donkey-hide gelatin into powder, pouring the powder into 120-150 parts of water, grinding, performing protease enzymolysis, inoculating aspergillus oryzae, and performing primary fermentation to obtain an intermediate fermentation product;

(3) adding the concentrated solution obtained in the step (1) into the intermediate fermentation product, uniformly stirring, inoculating streptococcus thermophilus, performing secondary fermentation, inoculating aspergillus oryzae, performing tertiary fermentation, centrifuging to obtain a supernatant, and performing spray drying to obtain the medicinal and edible enzyme suitable for patients with hyperuricemia or gout;

wherein, the compound enzyme comprises the following components in parts by weight: 1 part of flavourzyme, 0.5-0.8 part of cellulase, 0.3-0.4 part of ligninase, 0.3-0.4 part of amylase, 0.2-0.3 part of arabinosidase and 0.1-0.2 part of xylosidase.

2. The preparation method according to claim 1, wherein in the step (1), the mixed solvent is prepared by adding ethanol and isopropyl acetate into water and uniformly oscillating with ultrasonic waves.

3. The preparation method according to claim 1, wherein in the step (1), the flash crushing extractor is used for crushing and extracting, the extraction voltage is 150-200V, and the extraction time is 5-8 minutes.

4. The preparation method of claim 1, wherein in the step (1), the amount of the complex enzyme is 0.003-0.005 times of the weight of the platycodon grandiflorum, and the enzymolysis process conditions are as follows: the pH value is 5-6, the temperature is 50-55 ℃, and the enzymolysis time is 3-5 hours.

5. The preparation method according to claim 1, wherein in the step (1), low-temperature freeze concentration is adopted, and the specific temperature conditions are as follows: -30 to-35 ℃.

6. The preparation method according to claim 1, wherein in the step (2), the protease is subjected to enzymolysis by using flavourzyme, and the specific conditions are as follows: the pH value is 5-6, the temperature is 45-50 ℃, and the enzymolysis time is 2-3 hours.

7. The preparation method according to claim 1, wherein in the step (2), the inoculation amount of Aspergillus oryzae is 4-8%, and the process conditions of the first fermentation are as follows: fermenting for 8-10 hours at 30-35 ℃.

8. The preparation method according to claim 1, wherein in the step (3), the inoculation amount of the streptococcus thermophilus is 2-3%, and the process conditions of the second fermentation are as follows: fermenting for 3-4 hours at 40-42 ℃.

9. The preparation method according to claim 1, wherein in the step (3), the inoculation amount of Aspergillus oryzae is 6-8%, and the process conditions of the third fermentation are as follows: fermenting for 6-8 days at 30-35 ℃.

10. A homologous plant-food enzyme suitable for patients with hyperuricemia or gout, obtained by the preparation method of any one of claims 1 to 9.

Technical Field

The invention relates to the technical field of enzyme preparation, and particularly relates to a medicine-food homologous enzyme suitable for patients with hyperuricemia or gout and a preparation method thereof.

Background

Hyperuricemia is a disease characterized by in vivo purine metabolism disorder, increased uric acid production and/or decreased uric acid excretion, so that uric acid is deposited in vivo, and in severe cases, pathophysiological changes can be caused, and gout, uric acid nephropathy and the like are caused. Since the uric acid level produced by endogenous purine metabolism is substantially constant, the uric acid level in vivo is mainly influenced by the external ingestion of purine-or nucleic acid-rich protein-rich food. The domestic diagnosis standard is that male blood uric acid more than 417 mu mol/L (7.0mg/ml) and female blood uric acid more than 357 mu mol/L (6.0mg/ml) are called hyperuricemia. In recent years, with the improvement of living standard of people and the change of dietary structure, the prevalence rate of hyperuricemia in China has a very obvious rising trend.

Gout is acute and chronic inflammation and tissue injury caused by deposition of monosodium urate on bone joints, kidneys, subcutaneous parts and the like, is directly related to hyperuricemia caused by purine metabolic disorder and/or reduction of uric acid excretion, and belongs to the category of metabolic rheumatism. It is clinically characterized by acute and chronic arthritis and soft tissue injury which are caused by hyperuricemia and urate deposition and recurrent attacks, and gouty nephropathy caused by uric acid renal calculus. The key biochemical basis for gout attack is hyperuricemia, and research shows that about 5-10% of hyperuricemia can develop into gout, the prevalence rate of gout in coastal and developed areas is higher than that in inland areas, and males are higher than females.

At present, the prevalence rate of hyperuricemia and gout is increasing year by year, the suffering age is also tending to be younger, and people should pay attention to the disease. However, the symptoms of hyperuricemia and gout arthritis pain do not have any radical cure method, and only can relieve the pain of acute diseases. In the prior art, the gout is mainly treated by western medicines, traditional Chinese medicines, acupuncture and moxibustion, traditional Chinese medicine decoction, Chinese patent medicines, gout tea and the like. Western medicines and gout patients mainly take orally analgesic medicines, anti-inflammatory medicines and excretion medicines such as antibiotics, indomethacin, colchicine and allopurinol, play a role in temporarily relieving pain, and can also have the side effect of easily destroying stomach and damaging liver and kidney. The traditional Chinese medicine for treating gout takes clearing heat and promoting diuresis, promoting blood circulation and removing obstruction in channels, and inducing diuresis and discharging acid as guiding principles, but has higher requirements and great difficulty in diagnosis and treatment and diagnosis medication. The prescriptions for treating gout by differentiation of symptoms are different and have low universality. Meanwhile, the traditional Chinese medicine decoction also has the defect of troublesome decoction in the taking process; although the Chinese patent medicine is convenient to use, the last effect is not determined strongly and is difficult to popularize because the medicine can not be taken according to symptoms. Acupuncture and moxibustion, which is a technologically superior acupuncture and moxibustion practitioner, can well treat gout without side effects and with good stability after treatment, but the technical requirements of acupuncture and moxibustion treatment are high and the treatment time is long.

Among various inducing factors of gout, diet factors account for the first, so that the dietary nutrition intervention is reasonable to relieve the clinical symptoms of patients with hyperuricemia and gout, ensure the nutrition of organisms and improve the life quality of the patients, and has great significance.

Disclosure of Invention

The invention aims to provide a medicine-food homologous enzyme suitable for patients with hyperuricemia or gout and a preparation method thereof, which can effectively control index levels of blood uric acid and the like of patients with hyperuricemia or gout and can be eaten daily.

In order to achieve the purpose, the invention provides a preparation method of a medicinal and edible enzyme suitable for patients with hyperuricemia or gout, which comprises the following specific steps in parts by weight:

(1) firstly, 100 parts of platycodon grandiflorum, 30-40 parts of emblic leafflower fruit, 20-25 parts of kudzu vine root, 10-15 parts of microcos paniculata, 8-10 parts of long pepper and 5-8 parts of bunge cherry seed are respectively crushed into powder and mixed to obtain mixed powder, and then the mixed powder is prepared by the following steps of: 3-4: 100, extracting to obtain an extracting solution, performing enzymolysis by using complex enzyme, and concentrating to obtain a concentrated solution;

(2) respectively crushing 70-80 parts of zaocys dhumnade and 5-8 parts of donkey-hide gelatin into powder, pouring the powder into 120-150 parts of water, grinding, performing protease enzymolysis, inoculating aspergillus oryzae, and performing primary fermentation to obtain an intermediate fermentation product;

(3) adding the concentrated solution obtained in the step (1) into the intermediate fermentation product, uniformly stirring, inoculating streptococcus thermophilus, performing secondary fermentation, inoculating aspergillus oryzae, performing tertiary fermentation, centrifuging to obtain a supernatant, and performing spray drying to obtain the medicinal and edible enzyme suitable for patients with hyperuricemia or gout;

wherein, the compound enzyme comprises the following components in parts by weight: 1 part of flavourzyme, 0.5-0.8 part of cellulase, 0.3-0.4 part of ligninase, 0.3-0.4 part of amylase, 0.2-0.3 part of arabinosidase and 0.1-0.2 part of xylosidase.

Preferably, the raw materials are respectively crushed to 100-200 meshes.

Preferably, in the step (1), the mixed solvent is obtained by adding ethanol and isopropyl acetate into water and uniformly oscillating with ultrasonic waves.

Preferably, in the step (1), a flash crushing extractor is adopted for crushing and extracting, the extraction voltage is 150-200V, and the extraction time is 5-8 minutes.

Preferably, in the step (1), the amount of the complex enzyme is 0.003-0.005 time of the weight of the platycodon grandiflorum, and the enzymolysis process conditions are as follows: the pH value is 5-6, the temperature is 50-55 ℃, and the enzymolysis time is 3-5 hours.

Preferably, in the step (1), low-temperature freezing concentration is adopted, and the specific temperature conditions are as follows: -30 to-35 ℃.

Preferably, in the step (2), protease enzymolysis is performed by adopting flavourzyme, and the specific conditions are as follows: the pH value is 5-6, the temperature is 45-50 ℃, and the enzymolysis time is 2-3 hours.

Preferably, in the step (2), the inoculation amount of aspergillus oryzae is 4-8%, and the process conditions of the first fermentation are as follows: fermenting for 8-10 hours at 30-35 ℃.

Preferably, in the step (3), the inoculation amount of the streptococcus thermophilus is 2-3%, and the process conditions of the second fermentation are as follows: fermenting for 3-4 hours at 40-42 ℃.

Preferably, in the step (3), the inoculation amount of aspergillus oryzae is 6-8%, and the process conditions of the third fermentation are as follows: fermenting for 6-8 days at 30-35 ℃.

Preferably, in the step (3), a closed circulation spray drying method is adopted for drying, and the specific process conditions are as follows: heating nitrogen for drying to 150-180 ℃, and setting the air outlet temperature to 100-120 ℃, wherein the pressure of the nitrogen is 0.6-0.8 MPa, and the flow is 50-60 m³/h。

The medicine-food homologous enzyme is suitable for patients with hyperuricemia or gout.

The invention has the following beneficial effects:

the enzyme product is prepared from the raw materials of zaocys dhumnade, donkey-hide gelatin, platycodon grandiflorum, emblic leafflower fruit, kudzu vine root, microcos paniculata, long pepper, bunge cherry seed and the like, all the raw materials are listed in a medicine-food homology list published by Weijian, and the enzyme product can be eaten daily without toxic and side effects, effectively controls index levels of hyperuricemia or gout patients such as blood uric acid and the like, and has a good health-care effect. The medicinal and edible ferment obtained by the invention contains alkaloid, flavone, small molecular peptide and the like, on one hand, the components have a certain uric acid reducing effect when being used independently, and on the other hand, the small molecular peptide can carry the alkaloid and the flavone to rapidly enter blood to inhibit purine synthesis, so that the uric acid reducing effect is further enhanced.

The Zaocys dhumnades and donkey-hide gelatin contain rich protein, are degraded into small molecular peptides or amino acids through enzymolysis and fermentation treatment, can be directly absorbed through intestinal capillaries, and can transport the whole body to take effect, and can control PRPP amidotransferase synthesized by purine, thereby inhibiting purine synthesis and purine metabolism. The small molecular peptide or amino acid can interfere the enzyme which is used for converting purine into uric acid, such as xanthine oxidase, and the like, thereby controlling the content of the uric acid in the body.

Radix platycodonis and bunge cherry seed contain rich flavone, emblic leafflower fruit, kudzuvine root and long pepper extract contain rich alkaloid, and microcos paniculata contains blocked alkaloid and flavone, wherein the alkaloid can be specifically combined with uric acid to form a compound, can inhibit purine synthesis and purine metabolism, reduce the content of uric acid in vivo and eliminate uric acid; the flavone is a strong antioxidant, can effectively remove oxygen free radicals in vivo, prevents nucleic acid from being oxidized, and plays a role in reducing purine content; can reduce blood sugar and triglyceride, reduce capillary fragility, increase permeability, relieve renal cell pressure, and allow more uric acid to permeate. Meanwhile, the renal protein tubule can be stabilized, and the kidney is favorable for metabolizing uric acid.

The method is characterized in that platycodon grandiflorum, emblic leafflower fruit, kudzu vine root, microcos paniculata, long pepper and bunge cherry seed are prepared into mixed powder, and then extracted by using a mixed solvent of ethanol, isopropyl acetate and water, wherein the polarity of water is the largest, the polarity of isopropyl acetate is the smallest, and the three raw materials are matched to facilitate the dissolution of effective components in the raw materials (the effective components have large polarity difference, namely quaternary ammonium alkaloids, flavonols and the like with larger polarity and fatty amine alkaloids, isoflavones and the like with smaller polarity, according to the principle of similarity and intermiscibility, the water with the largest polarity facilitates the dissolution of quaternary ammonium alkaloids, flavonols and the like with larger polarity, the isopropyl acetate with the smallest polarity facilitates the dissolution of fatty amine alkaloids, isoflavones and the like with smaller polarity, and the effective components with different polarities are promoted to be dissolved through solvent combination). The compound enzyme composed of flavourzyme, cellulase, ligninase, amylase, arabinosidase, xylosidase and the like is used for enzymolysis, so that protein, cellulose, lignin, starch, sugar and the like in the raw materials can be degraded to generate small molecular peptides, amino acids, oligosaccharides and the like, and the blood uric acid level can be reduced.

The reason why the raw materials are divided into two parts for respective treatment is that the zaocys dhumnade and the donkey-hide gelatin are both animal-derived raw materials, contain a large amount of protein, are difficult to directly extract, and cannot produce beneficial substances (small molecular peptides, amino acids and the like generated by degrading macromolecular proteins) for reducing the level of the hematuria. The Zaocys dhumnades and donkey-hide gelatin are hydrolyzed by protease to degrade a part of macromolecular protein, inoculated into Aspergillus oryzae, and further degraded by protease, amylase, cellulase and the like produced by the fermentation of Aspergillus oryzae. Then adding concentrated solution generated by enzymolysis and fermentation of platycodon grandiflorum and the like, inoculating streptococcus thermophilus for secondary fermentation to generate lactic acid, wherein a small amount of lactic acid is beneficial to absorbing and metabolizing exogenous nucleoside and purine base, and is beneficial to further degrading macromolecular protein in the fermentation process, so that the beneficial small molecular peptide or amino acid is generated. Finally, aspergillus oryzae is inoculated for the third fermentation, protease, amylase, cellulase and the like are produced by the fermentation of the aspergillus oryzae, more thorough degradation is carried out, and small molecular substances which are beneficial to reducing the level of hematuria are produced.

In addition to the objects, features and advantages described above, other objects, features and advantages of the present invention are also provided. The present invention will be described in further detail below.

Detailed Description

The following is a detailed description of embodiments of the invention, but the invention can be implemented in many different ways, as defined and covered by the claims.