阅读说明：本技术 一种高纯度低聚木糖制备工艺 (Preparation process of high-purity xylo-oligosaccharide ) 是由 宋建民 王德海 宛荣生 张琴 王颂 黄祥君 于 2020-06-15 设计创作，主要内容包括：本发明公开了一种高纯度低聚木糖制备工艺,本发明具体包括以下步骤：S1、原料的选取和清洗,S2、木聚糖原料的提取,S3、生产原料的酶解处理,S4、菌种酶解处理,S5、除杂纯化,S6、高纯度低聚木糖的制备,本发明涉及食品加工技术领域。该高纯度低聚木糖制备工艺,可实现在对原料酶解前先对原料进行彻底的除杂处理,来提高低聚木糖的纯度,很好的达到了通过设置双螺旋搅拌机构,来加快酶解反应速度的目的,实现了通过对生产原料进行充分清洗,来对原料进行充分除杂,同时很好的达到了通过将生产原料中的聚木糖先提取出来再酶解生成低聚木糖,来提高低聚木糖制备纯度的目的,大大降低了低聚木糖中的杂质含量。(The invention discloses a preparation process of high-purity xylo-oligosaccharide, which specifically comprises the following steps: s1, selecting and cleaning raw materials, S2, extracting xylan raw materials, S3, performing enzymolysis treatment on production raw materials, S4, performing enzymolysis treatment on strains, S5, removing impurities and purifying, S6, and preparing high-purity xylo-oligosaccharide, and relates to the technical field of food processing. This high-purity xylo-oligosaccharide preparation technology, can realize carrying out thorough edulcoration processing to the raw materials earlier before the enzymolysis of raw materials, improve xylo-oligosaccharide's purity, fine reaching through setting up double helix rabbling mechanism, accelerate the purpose of enzymolysis reaction speed, realized through fully rinsing production raw materials, come to carry out abundant edulcoration to the raw materials, simultaneously fine reaching through with the raw materials for production poly xylose draw out earlier again enzymolysis generation xylo-oligosaccharide, improve xylo-oligosaccharide preparation purity's purpose, greatly reduced the impurity content in the xylo-oligosaccharide.)

1. A preparation process of high-purity xylo-oligosaccharide is characterized by comprising the following steps: the method specifically comprises the following steps:

s1, selecting and cleaning raw materials: firstly, weighing production raw materials to be processed in corresponding weight proportion by a weighing device, then pouring the production raw materials into a cleaning pool, injecting clear water, washing for 2-3 times, and then rinsing for 1-2 times, thereby obtaining cleaned production raw materials;

s2, extracting xylan raw materials: adding the production raw material cleaned in the step S1 into a cooking device, then injecting clear water, heating at low temperature for 10-20min after boiling at high temperature, then filtering the filter residue through a filtering device, transferring the filter residue into an extraction tank, then adding 10-15mL of alkaline extracting solution into the extraction tank, stirring for 10-20min at the rotation speed of 200 plus materials and 300r/min through a stirring device, and then filtering the filter residue through the filtering device to obtain the xylan raw material;

s3, enzymolysis treatment of production raw materials: transferring the xylan raw material prepared in the step S2 to a reaction tank, then sequentially adding 5-10mL of xylanase and 3-7mL of glucanase, starting a double-helix stirring device in the reaction tank, and stirring for 1-2h at the rotation speed of 500 plus 600r/min and the temperature of 46-70 ℃ for stirring reaction, thereby completing the enzymolysis treatment of the raw material;

s4, performing strain enzymolysis: inoculating enzymolysis strains into the production raw materials subjected to enzymolysis in the step S3 for fermentation, then sealing the fermentation tank for fermentation for 2-3 days, opening a cover, and heating to sterilize enzyme to obtain xylo-oligosaccharide enzymolysis liquid;

s5, impurity removal and purification: sequentially decoloring the xylo-oligosaccharide enzymolysis liquid obtained in the step S4 by using activated carbon and removing impurities and purifying by using ion exchange resin, and then concentrating the purified xylo-oligosaccharide sugar liquid by using concentration equipment until the concentration is 50-80%;

s6, preparation of high-purity xylo-oligosaccharide: and (4) transferring the xylo-oligosaccharide sugar solution concentrated in the step (S5) to drying equipment, carrying out vacuum drying at the temperature of 75-90 ℃, obtaining high-purity xylo-oligosaccharide crystals after drying, crushing, grinding and screening the high-purity xylo-oligosaccharide crystals to obtain high-purity xylo-oligosaccharide powder, and finally packaging by packaging equipment to obtain the high-purity xylo-oligosaccharide finished product.

2. The process for preparing high-purity xylo-oligosaccharide according to claim 1, wherein the process comprises the following steps: the production raw materials in the step S1 are corn cob, bagasse, cottonseed hull, oat and birch mixture.

3. The process for preparing high-purity xylo-oligosaccharide according to claim 1, wherein the process comprises the following steps: the alkaline extracting solution in the step S2 is 0.3-0.6mol/L sodium hydroxide solution.

4. The process for preparing high-purity xylo-oligosaccharide according to claim 1, wherein the process comprises the following steps: and in the step S3, spiral stirring mechanisms are arranged at the top and the bottom of the reaction tank, and the stirring assemblies are driven by two servo motors to rotate, so that the reaction liquid in the reaction tank is fully mixed and reacted.

5. The process for preparing high-purity xylo-oligosaccharide according to claim 1, wherein the process comprises the following steps: the enzyme activity ratio in the step S3 is 3-8: 1.

6. The process for preparing high-purity xylo-oligosaccharide according to claim 1, wherein the process comprises the following steps: the zymolytic strain in the step S4 is one or a combination of streptomycete, aspergillus, penicillium, trichoderma or chaetomium.

Technical Field

The invention relates to the technical field of food processing, in particular to a preparation process of high-purity xylo-oligosaccharide.

Background

Xylo-oligosaccharide is also called xylo-oligosaccharide, it is functional polymeric sugar that is formed by 2-7 xylose molecules through beta-1, 4 glycosidic bond, its bifidus factor function is 10-20 times of other polymeric sugar, xylo-oligosaccharide has unique advantage compared with soybean oligosaccharide, fructo-oligosaccharide, isomaltose oligosaccharide, etc. that people usually use, it can promote the multiplication activity of intestinal bifidobacteria selectively, xylo-oligosaccharide is difficult to be decomposed by human digestive enzyme, digestion experiment carried on with saliva, gastric juice, pancreatic juice and intestinal enzyme liquid shows: the xylo-oligosaccharide can not decompose almost all digestive juice, the energy value of the xylo-oligosaccharide is almost zero, the xylo-oligosaccharide does not affect the blood sugar concentration, does not increase the insulin level in the blood sugar, and does not form fat deposition, so the xylo-oligosaccharide can play a role in low-energy food, and meets the requirements of people who love sweetmeats and worry about diabetes and obesity to the maximum extent, so that the xylo-oligosaccharide can be eaten by diabetes patients, obesity patients and hypoglycemia patients at ease, has good acid and heat stability and is difficult to ferment, compared with other oligosaccharides, the xylo-oligosaccharide has the outstanding characteristic of good stability, basically does not decompose even when being heated to 100 ℃ under an acid condition (pH is 2.5-7), and certain functional oligosaccharides are easy to decompose under the acid condition, so the proliferation activity of bifidobacterium is reduced.

At present, xylo-oligosaccharide's preparation method, it is most directly to adopt enzymolysis method to prepare, however, such preparation method impurity content is higher, the purity is lower, and production efficiency is lower, can not realize carrying out thorough edulcoration processing to the raw materials earlier before the enzymolysis of raw materials, improve xylo-oligosaccharide's purity, can't reach through setting up double helix rabbling mechanism, accelerate enzymolysis reaction speed's purpose, can not realize through fully rinsing production raw materials, come to carry out abundant edulcoration to the raw materials, can't reach simultaneously through extracting out firstly xylo-oligosaccharide in the production raw materials and then enzymolysis production xylo-oligosaccharide, improve xylo-oligosaccharide preparation purity's purpose, thereby the xylo-oligosaccharide preparation work that gives producers has brought very big inconvenience.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a preparation process of high-purity xylo-oligosaccharide, which solves the problems that the existing preparation method has high impurity content, low purity and low production efficiency, and simultaneously, the aim of improving the preparation purity of the xylo-oligosaccharide can not be achieved by extracting the xylo-oligosaccharide in the production raw materials firstly and then carrying out enzymolysis to generate the xylo-oligosaccharide.

In order to achieve the purpose, the invention is realized by the following technical scheme: a preparation process of high-purity xylo-oligosaccharide specifically comprises the following steps:

s1, selecting and cleaning raw materials: firstly, weighing production raw materials to be processed in corresponding weight proportion by a weighing device, then pouring the production raw materials into a cleaning pool, injecting clear water, washing for 2-3 times, and then rinsing for 1-2 times, thereby obtaining cleaned production raw materials;

s2, extracting xylan raw materials: adding the production raw material cleaned in the step S1 into a cooking device, then injecting clear water, heating at low temperature for 10-20min after boiling at high temperature, then filtering the filter residue through a filtering device, transferring the filter residue into an extraction tank, then adding 10-15mL of alkaline extracting solution into the extraction tank, stirring for 10-20min at the rotation speed of 200-300r/min through a stirring device, and then filtering the filter residue through the filtering device to obtain the xylan raw material;

s3, enzymolysis treatment of production raw materials: transferring the xylan raw material prepared in the step S2 to a reaction tank, then sequentially adding 5-10mL of xylanase and 3-7mL of glucanase, starting a double-helix stirring device in the reaction tank, and stirring for 1-2h at the rotation speed of 500 plus 600r/min and the temperature of 46-70 ℃ for stirring reaction, thereby completing the enzymolysis treatment of the raw material;

s4, performing strain enzymolysis: inoculating enzymolysis strains into the production raw materials subjected to enzymolysis in the step S3 for fermentation, then sealing the fermentation tank for fermentation for 2-3 days, opening a cover, and heating to sterilize enzyme to obtain xylo-oligosaccharide enzymolysis liquid;

s5, impurity removal and purification: sequentially decoloring the xylo-oligosaccharide enzymolysis liquid obtained in the step S4 by using activated carbon and removing impurities and purifying by using ion exchange resin, and then concentrating the purified xylo-oligosaccharide sugar liquid by using concentration equipment until the concentration is 50-80%;

s6, preparation of high-purity xylo-oligosaccharide: and (4) transferring the xylo-oligosaccharide sugar solution concentrated in the step (S5) to drying equipment, carrying out vacuum drying at the temperature of 75-90 ℃, obtaining high-purity xylo-oligosaccharide crystals after drying, crushing, grinding and screening the high-purity xylo-oligosaccharide crystals to obtain high-purity xylo-oligosaccharide powder, and finally packaging by packaging equipment to obtain the high-purity xylo-oligosaccharide finished product.

Preferably, the raw material for production in step S1 is a mixture of corncob, bagasse, cottonseed hull, oat, and birch.

Preferably, the alkaline extract in step S2 is 0.3-0.6mol/L sodium hydroxide solution.

Preferably, in the step S3, spiral stirring mechanisms are installed at the top and the bottom of the reaction tank, and the stirring assemblies are driven by two servo motors to rotate, so that the reaction solution in the reaction tank is fully mixed and reacted.

Preferably, the enzyme activity ratio in the step S3 is 3-8: 1.

Preferably, the zymolytic species in step S4 is one or more of streptomyces, aspergillus, penicillium, trichoderma, or chaetomium.

The invention provides a preparation process of high-purity xylo-oligosaccharide. Compared with the prior art, the method has the following beneficial effects: the preparation process of the high-purity xylo-oligosaccharide specifically comprises the following steps: s1, selecting and cleaning raw materials: firstly, weighing production raw materials to be processed in corresponding weight ratio by a weighing device, then pouring the production raw materials into a cleaning pool, injecting clear water, washing for 2-3 times, then washing for 1-2 times, thus obtaining cleaned production raw materials, and S2, extracting xylan raw materials: adding the production raw material cleaned in the step S1 into a cooking device, then injecting clear water, heating at a low temperature for 10-20min after boiling at a high temperature, then filtering the filter residue through a filtering device, transferring the filter residue into an extraction tank, then adding 10-15mL of alkaline extracting solution into the extraction tank, stirring for 10-20min at a rotation speed of 200 plus 300r/min through a stirring device, and then filtering the filter residue through the filtering device, thus obtaining the xylan raw material, S3, carrying out enzymolysis treatment on the production raw material: transferring the xylan raw material prepared in the step S2 to a reaction tank, then sequentially adding 5-10mL of xylanase and 3-7mL of glucanase, starting a double-helix stirring device in the reaction tank, stirring for 1-2h at the rotation speed of 500 plus 600r/min and the temperature of 46-70 ℃ for stirring reaction, thereby completing the enzymolysis treatment of the raw material, S4, strain enzymolysis treatment: inoculating enzymolysis strains into the production raw materials subjected to enzymolysis in the step S3 for fermentation, then opening a cover to heat up the sterilization enzyme after fermenting for 2-3 days in a sealed fermentation tank, thereby obtaining xylo-oligosaccharide enzymolysis liquid, S5, removing impurities and purifying: and (3) sequentially carrying out activated carbon decoloration and ion exchange resin impurity removal purification on the xylo-oligosaccharide enzymolysis liquid obtained in the step (S4), concentrating the purified xylo-oligosaccharide sugar liquid to 50-80% by using concentration equipment, and preparing high-purity xylo-oligosaccharide by using S6: transferring the xylo-oligosaccharide sugar solution concentrated in the step S5 into drying equipment, carrying out vacuum drying at the temperature of 75-90 ℃, obtaining high-purity xylo-oligosaccharide crystals after drying, then crushing, grinding and screening the high-purity xylo-oligosaccharide crystals to obtain high-purity xylo-oligosaccharide powder, finally packaging by packaging equipment to obtain high-purity xylo-oligosaccharide finished products, and realizing thorough impurity removal treatment on raw materials before enzymolysis of the raw materials to improve the purity of the xylo-oligosaccharide, well achieving the purpose of accelerating the enzymolysis reaction speed by arranging a double-helix stirring mechanism, fully cleaning the production raw materials to fully remove impurities from the raw materials, and simultaneously well achieving the purpose of generating xylo-oligosaccharide by extracting the xylo-oligosaccharide in the production raw materials and then carrying out enzymolysis, the method has the advantages of improving the purity of the xylo-oligosaccharide preparation, greatly reducing the impurity content in the xylo-oligosaccharide, and well improving the purity and the production efficiency of the xylo-oligosaccharide, thereby greatly facilitating the preparation work of the xylo-oligosaccharide by production personnel.

Drawings

FIG. 1 is a flow chart of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1, the embodiment of the present invention provides three technical solutions: a preparation process of high-purity xylo-oligosaccharide specifically comprises the following embodiments: