阅读说明：本技术 一种大熊猫尿液激素的校正方法 (Correction method of panda urine hormone ) 是由 黄河 侯蓉 张志和 胡贤彪 张东升 王成东 兰景超 黄祥明 于 2020-07-10 设计创作，主要内容包括：本发明公开了一种大熊猫尿液激素的校正方法,所述校正方法包括如下步骤：采集大熊猫尿液；测定大熊猫尿液中的原始激素浓度以及尿比重；按照校正结果＝原始激素浓度/Lg尿比重/500000对原始激素浓度进行校正,得到校正结果；本发明对大熊猫尿液测定的尿比重值直接采用函数转换后进行激素尿比重校正,消除了平均尿比重值存在的代表性不足的问题,且无需事先对特定大熊猫进行尿液采集和计算平均尿比重值,只需对具体尿液进行尿比重测定后,运用公式直接校正激素水平,该校正方法操作简单,校正快捷且准确性好。(The invention discloses a correction method of panda urine hormone, which comprises the following steps: collecting panda urine; measuring the original hormone concentration and the urine specific gravity of the panda urine; correcting the original hormone concentration according to the correction result, namely the original hormone concentration/Lg urine specific gravity/500000 to obtain a correction result; the invention directly adopts function conversion to the urine specific gravity value measured by the panda urine and then carries out hormone urine specific gravity correction, eliminates the problem of insufficient representativeness existing in the average urine specific gravity value, does not need to carry out urine collection and calculation on the average urine specific gravity value in advance, only needs to carry out urine specific gravity measurement on specific pandas and then directly corrects the hormone level by using a formula, and the correction method has simple operation, quick correction and good accuracy.)

1. A correction method of giant panda urine hormone is characterized by comprising the following steps:

(a) collecting panda urine;

(b) measuring the original hormone concentration and the urine specific gravity of the panda urine;

(c) the original hormone concentration was corrected by the correction result of original hormone concentration/Lg urine specific gravity/500000 to obtain a correction result.

2. Correction method according to claim 1, characterized in that the hormone is an estrogen and/or a progestin.

3. The calibration method according to claim 1, wherein the method for measuring the specific gravity of urine is a densitometer method or a dry chemistry strip method.

4. The calibration method according to claim 3, wherein the hydrometer method determining the specific gravity of urine includes:

the urine hydrometer is checked and corrected, then the urine hydrometer of the panda urine is measured, and after the measurement is finished, the urine hydrometer is cleaned.

5. The correction method according to claim 4, wherein the verifying the correction comprises:

deionized water is added into a sample groove of the uropycnometer, and the deionized water in the sample groove is sucked out when the reading of the uropycnometer is fixed to be 1.000.

6. The calibration method according to claim 4, wherein the cleaning solution used in the cleaning is deionized water, and the reading of the uropycnometer is fixed to 1.000 after the deionized water is added.

7. The calibration method according to claim 1, wherein said original hormone concentration is determined by enzyme-linked immunosorbent assay.

8. The calibration method according to claim 1, wherein the panda urine collection method comprises:

cleaning and drying the ground of the animal house;

putting the pandas into a animal house, collecting urine by using an injector once the pandas discharge urine, filling the urine into a freezing pipe, and immediately putting the freezing pipe into a refrigerator for freezing or refrigerating.

Technical Field

The invention relates to the technical field of correction of panda urine hormones, in particular to a correction method of panda urine hormones.

Background

The concentration of substances in urine is influenced by drinking water, urine volume, food and other factors, and in order to eliminate the influence, the method can be used for correcting by a 24-hour urine collecting method, a flow rate correcting method, a creatinine correcting method and a specific gravity correcting method, but the 24-hour urine collecting method and the flow rate correcting method are inconvenient to operate and are less adopted.

In the management of captive pandas, the levels of reproductive hormones such as estrogen, progestogen and the like in urine samples of corresponding individual pandas in different time periods are detected, and the changes of the relevant hormones such as estrogen, progestogen and the like in each time period are contrastively analyzed, so that whether the corresponding pandas estrus or not and the stage of estrus are judged, and further, specific feeding, reproduction management and other implementation schemes are formulated in the management. However, the concentration of hormones in the urine of pandas is influenced by the above factors, and in order to make the hormone levels in the urine of different concentrations comparatively analyzable, the hormone levels are corrected by creatinine in the actual hormone measurement, and the change of estrogen, progestogen and the like in the urine of the pandas at different time points is comparatively analyzed according to the results of the hormones corrected by the creatinine.

The creatinine correction result of the panda urine hormone plays a great role in panda management and is widely applied to a panda core population management unit; however, mechanisms such as Chinese panda breeding and exhibition and the like are provided with few pandas, usually 2-4 pandas, and corresponding detection departments are not arranged for measuring the creatinine of the panda urine; on the other hand, the chemical reagents required for creatinine determination include: picric acid, trinitrophenol, is a flammable and explosive agent that requires special approval from the local police department for purchase and transport; the other is sodium hydroxide which is strong alkali, and except for scientific research requirements, the chemical reagents are not purchased by institutions such as general panda breeding and exhibition; therefore, in the breeding season of pandas every year, a panda adult breeding research base sends hormone testers to the panda breeding and developing institution in China for hormone measurement. However, the reagent for measuring creatinine, which carries picric acid, is limited. Therefore, in the aspect of correction of the urine hormone creatinine of the pandas, domestic related panda feeding mechanisms can only resort to local colleges and universities or hospitals, as the colleges and the hospitals are usually far away from the feeding mechanisms, a large amount of time is spent on sample feeding, or as the colleges and the hospital staff cannot timely detect the urine creatinine content due to work arrangement, the correction result of the urine hormone cannot be timely obtained.

Currently, Jella Wauters (2018) reports correction of uropycnometry for urohormone levels in pandas, the correction formula is: and the hormone urine specific gravity correction result is original hormone concentration x [ (average urine specific gravity value-1.000)/(target sample urine specific gravity value-1.000) ], and the average urine specific gravity value refers to an average urine specific gravity value calculated after detecting not less than 30 urine samples collected from a specific panda individual. In the formula, before hormone urine specific gravity correction is carried out on a specific panda individual, the average urine specific gravity value of the individual in the past needs to be obtained. Because the urine concentration and the urine output of pandas are affected by the ingestion of pandas (for example, the ingestion of different types of bamboos in different seasons and different parts of the bamboos such as bamboo stems, bamboo leaves and bamboo shoots), the amount of exercise and physiological changes in estrus and rest periods, the drinking conditions and the like, the average value of the urine specific gravity calculated by collecting no less than 30 urine samples for detection in the report is not enough to represent the real average value of the urine specific gravity of a particular individual panda in each season and various physiological conditions. If a representative average urine specific gravity value needs to be obtained, the average urine specific gravity value needs to be acquired and measured for a specific panda in different seasons, different foods, different physiological conditions and the like, so that long-term urine acquisition and urine specific gravity detection can be performed on the panda. Meanwhile, in the management of pandas, the hormone level is often temporarily detected and corrected for a specific individual, and for pandas for which urine sample collection and average urine specific gravity value calculation have not been performed in advance, the hormone correction formula cannot be adopted.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides a correction method of panda urine hormone, which is simple to operate, quick to correct and good in accuracy.

In order to achieve the above purpose of the present invention, the following technical solutions are adopted:

the invention provides a correction method of panda urine hormone, which comprises the following steps:

(a) collecting panda urine;

(b) measuring the original hormone concentration and the urine specific gravity of the panda urine;

(c) the original hormone concentration was corrected by the correction result of original hormone concentration/Lg urine specific gravity/500000 to obtain a correction result.

The invention directly adopts function conversion to the urine specific gravity value measured by the panda urine and then carries out hormone urine specific gravity correction, thereby eliminating the problem of insufficient representativeness existing in the average urine specific gravity value, and the urine collection and the calculation of the average urine specific gravity value are not needed to be carried out on the specific panda in advance; specifically, the correlation r of the correction method and the creatinine correction method to the estrogen correction result is 0.968, and the correlation r to the progestogen correction result is 0.98.

The correction result of the present invention, original hormone concentration/Lg specific gravity of urine/500000, is specifically: corrected result-original hormone concentration divided by Lg urine specific gravity, divided by 500000; wherein the specific gravity of Lg is logarithmic of the specific gravity value of urine with 10 as a base.

Preferably, the hormone is an estrogen and/or a progestin.

The method for measuring the specific gravity of urine in the present invention is not limited strictly, and a conventional method for measuring the specific gravity of urine in the art may be selected according to actual needs.

More preferably, the hydrometer method determining the specific gravity of urine comprises:

the urine hydrometer is checked and corrected, then the urine hydrometer of the panda urine is measured, and after the measurement is finished, the urine hydrometer is cleaned.

The urine hydrometer is checked and corrected firstly, so that the accuracy of the urine hydrometer value measuring result can be guaranteed, and the accuracy of urine hormone correction is further guaranteed better.

Preferably, the verification correction comprises:

deionized water is added into a sample groove of the uropycnometer, and the deionized water in the sample groove is sucked out when the reading of the uropycnometer is fixed to be 1.000.

Preferably, the cleaning solution adopted by the cleaning is deionized water, and the reading of the uropycnometer is fixed to be 1.000 after the deionized water is added.

The method for determining the original hormone concentration in the panda urine is not strictly limited, and the conventional determination method of the hormone in the urine in the field can be adopted, for example, the content of the hormone in the panda urine can be determined by adopting an enzyme-linked immunosorbent assay; specifically, the method for detecting the original progestogen content in the urine of the panda disclosed in the specific embodiment of the chinese patent application with the patent application number CN201310092282.8 can be adopted; the method for detecting the original estrogen content in the urine of the panda can be adopted, which is disclosed in the specific embodiment of the Chinese patent application with the patent application number of 201310092471.5.

Preferably, the panda urine collection method comprises the following steps:

cleaning and drying the ground of the animal house;

putting the pandas into a animal house, collecting urine by using an injector once the pandas discharge urine, filling the urine into a freezing pipe, and immediately putting the freezing pipe into a refrigerator for freezing or refrigerating.

By adopting the method to collect the panda urine, the panda can be prevented from being polluted, and the urine can be exposed in the air for a long time to influence the content of each substance in the urine.

The invention has the beneficial effects that:

the invention directly adopts function conversion to the urine specific gravity value measured by the panda urine and then carries out hormone urine specific gravity correction, thereby eliminating the problem of insufficient representativeness existing in the average urine specific gravity value, and the urine collection and the calculation of the average urine specific gravity value are not needed to be carried out on the specific panda in advance; specifically, the correlation r of the correction method and the creatinine correction method to the estrogen correction result is 0.968, and the correlation r to the progestogen correction result is 0.98.

Drawings

In order to more clearly illustrate the detailed description of the invention or the technical solutions in the prior art, the drawings that are needed in the detailed description of the invention or the prior art will be briefly described below. Throughout the drawings, like elements or portions are generally identified by like reference numerals. In the drawings, elements or portions are not necessarily drawn to scale.

FIG. 1 is a correlation analysis of correction results of estrogen in panda urine by creatinine correction and urine specific gravity correction in the experimental example of the present invention;

FIG. 2 is a correlation analysis of the correction results of progestogen in panda urine by creatinine correction and urine specific gravity correction in the experimental example of the present invention.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.

It is to be noted that, unless otherwise specified, technical or scientific terms used herein shall have the ordinary meaning as understood by those skilled in the art to which the invention pertains.