阅读说明：本技术 一种疟疾检测装置 (Malaria detection device ) 是由 金小峰 李建平 白雪 陈韶敏 丁梦如 何立栋 温建明 万嫩 宋振忠 蔡俊杰 于 2020-07-15 设计创作，主要内容包括：本发明属于疟疾检测领域,具体涉及一种疟疾检测装置。本发明解决了现有疟疾诊断质量不足,费时费力,且不适用于大规模样品检测的难题。该疟疾检测装置由包容装置、保温装置、控制器、流速测量装置、流体流动装置、检测装置、废液收集装置、连接管构成。本发明基于生物阻抗谱原理,通过检测血液样本中单个细胞的阻抗特性来判断是否感染疟疾及其感染程度。该装置结构简单,检测方便,易于微型化,移动化,适用于大批量样品的疟疾检测,可实现疟疾的快速高效检测。(The invention belongs to the field of malaria detection, and particularly relates to a malaria detection device. The invention solves the problems of insufficient quality, time and labor waste and inapplicability to large-scale sample detection of the existing malaria diagnosis. The malaria detection device comprises a containing device, a heat preservation device, a controller, a flow velocity measuring device, a fluid flowing device, a detection device, a waste liquid collecting device and a connecting pipe. The invention is based on the principle of bioimpedance spectroscopy, and judges whether malaria is infected and the infection degree of the malaria by detecting impedance characteristics of single cells in a blood sample. The device simple structure detects the convenience, easily miniaturation, and the removal is applicable to the malaria detection of big batch sample, can realize the high efficiency detection of malaria.)

1. A malaria detection device is characterized by comprising a containing device, a heat preservation device, a controller, a flow velocity measuring device, a fluid flowing device, a detection device, a waste liquid collecting device and a connecting pipe, wherein the containing device is provided with a blood injection port, a display device and a positioning column; the blood injection port, the heat preservation device, the fluid flowing device, the detection device and the waste liquid collecting device on the containing device are sequentially connected by connecting pipes; the flow rate measuring device is arranged on the connecting pipe and is used for measuring the flow rate of the fluid; the containing device is hollow and can contain parts and components, and can be carried movably; the fluid flow device functions to move fluid, such as a pump; the heat preservation device keeps the substance in the device at a certain temperature, such as a thermostat; the detection device is used for detecting the electrical impedance characteristic of single cells in blood, and the width of a channel is 20-30 um, such as a microfluidic chip; the controller is used for realizing signal sending, receiving and processing, the controller stores a calibrated single-cell electrical impedance parameter database in blood infected with malaria, and during data processing, the controller provides whether the blood sample is infected with malaria and the infection degree based on the matching of the detected measured blood cell electrical impedance characteristic signal and the stored database; the display device is used for receiving and displaying the image-text signal; the positioning column is used for installing and positioning the waste liquid collecting device on the containing device, and the waste liquid collecting device can be moved out of the containing device when the waste liquid is treated.

Technical Field

The invention relates to the technical field of malaria detection, in particular to a malaria detection device.

Background

Malaria is an ancient disease occurring along with human origin, and global malaria report 2019 issued by world health organization madly abused in tropical and subtropical regions to date indicates that about 2.28 hundred million cases of global malaria in 2018 are estimated, and 40.5 million cases of death occur.

Early and accurate diagnosis of malaria is crucial for effective disease management and malaria monitoring. High quality malaria diagnosis is critical in all cases because misdiagnosis can cause a significant amount of morbidity and mortality. At present, microscopy is still the gold standard for diagnosing malaria, and although microscopy is still the backbone for making parasitological diagnoses in most large medical clinics and hospitals, the quality of microscopic diagnosis is often insufficient, requiring the examiner to have professional knowledge and experience, and being time-consuming, labor-consuming and not suitable for the detection of large-scale samples, which negatively affects health results and optimal utilization of resources.

Rapid diagnosis of malaria can greatly improve the quality of treatment of malaria infection, especially in remote areas where there is a limit to obtaining good quality microscopic service. Patent CN207012179U proposes a malaria detector, which provides a method for rapidly diagnosing whether malaria is infected by malaria through raman spectroscopy analysis by irradiating human blood through skin with laser light of a specific wavelength, wherein hemozoin generated in human body by malaria parasites in blood generates raman scattering due to absorption of laser light; patent CN110832319A proposes a method for detecting possible malaria infection in a patient using digital light microscopy. The bioimpedance spectrum has the characteristics of rapidness/high efficiency, strong biological resolution and no radiation damage, and the bioimpedance spectrum has no related application in the malaria detection direction at present.

Disclosure of Invention

The invention aims to provide a malaria detection device based on bioimpedance spectroscopy, and solves the problems in the prior art. The invention realizes the portability and the miniaturization of structural design, has strong biological resolution and can realize quick and efficient detection.

The above object of the present invention is achieved by the following technical solutions:

a malaria detection device comprises a containing device, a heat preservation device, a controller, a flow velocity measurement device, a fluid flow device, a detection device, a waste liquid collection device and a connecting pipe, wherein the containing device is provided with a blood injection port, a display device and a positioning column; the blood injection port, the heat preservation device, the fluid flowing device, the detection device and the waste liquid collecting device on the containing device are sequentially connected by connecting pipes; the flow rate measuring device is arranged on the connecting pipe and is used for measuring the flow rate of the fluid; the containing device is hollow and can contain parts and components, and can be carried movably; the fluid flow device functions to move fluid, such as a pump; the heat preservation device keeps the substance in the device at a certain temperature, such as a thermostat; the detection device is used for detecting the electrical impedance characteristic of single cells in blood, and the width of a channel is 20-30 um, such as a microfluidic chip; the controller is used for realizing signal sending, receiving and processing, the controller stores a calibrated single-cell electrical impedance parameter database in blood infected with malaria, and during data processing, the controller provides whether the blood sample is infected with malaria and the infection degree based on the matching of the detected measured blood cell electrical impedance characteristic signal and the stored database; the display device is used for receiving and displaying the image-text signal; the positioning column is used for installing and positioning the waste liquid collecting device on the containing device, and the waste liquid collecting device can be moved out of the containing device when the waste liquid is treated.

The main advantages of the invention are: malaria is detected based on the bioimpedance spectrum, the biological resolution is strong, and rapid and efficient detection can be realized; meanwhile, the device has the advantages of simple structure, convenient detection, miniaturization and movability, and is suitable for malaria detection of large-batch samples.

Drawings

Fig. 1 is a schematic structural view of the present invention.

In the figure:

1. a containment device; 2. a heat preservation device; 3. a fluid flow device; 4. a flow rate measuring device; 5. a controller; 6. a detection device;

7. a waste liquid collection device; 8. a connecting pipe I; 9. a connecting pipe II; 10. a connecting pipe III; 11. a connecting pipe IV; 1-1, a blood injection port;

1-2. a display device; 1-3, positioning column.

Detailed Description

The details of the present invention and its embodiments are further described below with reference to the accompanying drawings.

Referring to fig. 1, a malaria detection device comprises a containing device (1), a heat preservation device (2), a controller (5), a flow rate measurement device (3), a fluid flow device (4), a detection device (6), a waste liquid collection device (7), a connecting pipe I (8), a connecting pipe II (9), a connecting pipe III (10), a connecting pipe IV (11), and a connecting pipe V (12), wherein the containing device (1) is provided with a blood injection port (1-1), a display device (1-2), and a positioning column (1-3), and the controller (5), the heat preservation device (2), the fluid flow device (3), and the detection device (6) are fixed in the containing device (1); the blood injection port (1-1), the heat preservation device (2), the fluid flowing device (3), the detection device (6) and the waste liquid collection device (7) on the containing device (1) are sequentially connected by a connecting pipe I (8), a connecting pipe II (9), a connecting pipe III (10) and a connecting pipe IV (11); the flow velocity measuring device (4) is arranged on the connecting pipe III (10) and is used for measuring the flow velocity of the fluid; the containing device (1) is hollow and can contain parts and components, and can be carried movably; the fluid flow means (3) functions to cause fluid to flow, such as a pump; the heat preservation device (2) keeps the substance in the device at a certain temperature, such as a thermostat; the detection device (6) is used for detecting the electrical impedance characteristic of single cells in blood, and the width of a channel is 20-30 um, such as a microfluidic chip; the controller (5) is used for realizing signal sending, receiving and processing, the controller (5) stores a calibrated single-cell electrical impedance parameter database in blood infected with malaria, and when the data are processed, the controller (5) matches the detected measured blood cell electrical impedance characteristic signal with the stored database to provide whether the blood sample is infected with malaria and the infection degree; the display device (1-2) is used for receiving and displaying the image-text signals; the positioning columns (1-3) are used for installing and positioning the waste liquid collecting device (7) on the containing device (1), and the waste liquid collecting device (7) can be moved out of the containing device (1) when waste liquid is treated.

Referring to fig. 1, the specific working process of the present invention is as follows:

the method comprises the following steps: the collected blood is injected into the heat preservation device (2) through the blood injection port (1-1), and the temperature of the heat preservation device (2) is kept at 37.5 ℃, so that the aim of maintaining the activity of cells is fulfilled;

step two: the controller (5) sends an electric signal to the fluid flowing device (3), and the fluid flowing device (3) conveys the blood in the heat preservation device (2) to the detection device (6);

step three: when the blood flow rate in the connecting pipe III (10) reaches the blood flow rate in the human body, the controller (5) sends an electric signal to the detection device (6) to detect the electrical impedance characteristics of the blood single cell;

step IV: the detection device (6) feeds the measured electric signal back to the controller (5);

step five: the controller (5) compares and fits the received electric signals with a prestored single-cell electrical impedance database in the blood sample infected with malaria, judges whether the blood sample is infected by malaria or not and the infection degree, sends the result to the display device (1-2), and the waste liquid collecting device (7) collects the blood detected by the detection device (6).

The invention relates to a malaria detection apparatus, which utilizes a biological impedance spectrum to judge whether malaria is infected and the infection degree of the malaria by detecting the impedance characteristics of single cells in a blood sample. The device simple structure detects the convenience, easily miniaturation, and the removal is applicable to the malaria detection of big batch sample, can realize the high efficiency detection of malaria.