阅读说明：本技术 一种抗菌无磷清洗剂及其制备方法和应用 (Antibacterial phosphorus-free cleaning agent and preparation method and application thereof ) 是由 左展 黄涛 张娟丽 于 2020-07-27 设计创作，主要内容包括：本发明提出了一种抗菌无磷清洗剂及其制备方法和应用,所述抗菌无磷清洗剂,每1L抗菌无磷清洗剂中含有：壬基酚聚氧乙烯醚TX-7 10-20ml,壬基酚聚氧乙烯醚TX-12 20-45ml,单乙醇胺10-17ml,硼酸2-4g,无水乙醇10-30ml,甲苯-4-磺酸钠1-2g,其余为去离子水。本发明适用于全自动生化分析仪,该抗菌无磷清洗剂外观为无色透明液体,化学性质稳定,在保证测试效果的同时降低了使用成本。(The invention provides an antibacterial phosphorus-free cleaning agent and a preparation method and application thereof, wherein each 1L of the antibacterial phosphorus-free cleaning agent comprises the following components: nonylphenol polyoxyethylene ether TX-710-20 ml, nonylphenol polyoxyethylene ether TX-1220-45 ml, monoethanolamine 10-17ml, boric acid 2-4g, absolute ethyl alcohol 10-30ml, toluene-4-sodium sulfonate 1-2g and the balance of deionized water. The antibacterial phosphorus-free cleaning agent is suitable for a full-automatic biochemical analyzer, is colorless and transparent liquid in appearance, is stable in chemical property, and reduces the use cost while ensuring the test effect.)

1. An antibacterial phosphorus-free cleaning agent, which is characterized in that each 1L of the antibacterial phosphorus-free cleaning agent contains: nonylphenol polyoxyethylene ether TX-710-20 ml, nonylphenol polyoxyethylene ether TX-1220-45 ml, monoethanolamine 10-17ml, boric acid 2-4g, absolute ethyl alcohol 10-30ml, toluene-4-sodium sulfonate 1-2g and the balance of deionized water.

2. The antibacterial phosphorus-free cleaning agent according to claim 1, wherein each 1L of antibacterial phosphorus-free cleaning agent comprises: nonylphenol polyoxyethylene ether TX-715ml, nonylphenol polyoxyethylene ether TX-1230 ml, monoethanolamine 14ml, boric acid 3g, absolute ethyl alcohol 20ml, toluene-4-sodium sulfonate 1.5g and the balance of deionized water.

3. A method for preparing the antibacterial phosphorus-free cleaning agent as defined in claim 1 or 2, which comprises the following steps:

(1) adding monoethanolamine into deionized water to obtain a solution A, dissolving boric acid into absolute ethyl alcohol, uniformly mixing to obtain a solution B, adding the solution B into the solution A, and uniformly stirring to obtain a premixed solution;

(2) adding toluene-4-sodium sulfonate into the premixed solution obtained in the step (1), and uniformly stirring to obtain a mixed solution;

(3) and (3) adding nonylphenol polyoxyethylene ether TX-7 and nonylphenol polyoxyethylene ether TX-12 into the mixed solution obtained in the step (2), adding deionized water to a constant volume of 1L, uniformly stirring, and filtering to obtain the antibacterial phosphorus-free cleaning agent.

4. The method according to claim 3, wherein the filtration in the step (2) is performed using a nitrocellulose filter having a pore size of 0.22 um.

5. The antibacterial phosphorus-free cleaning agent as claimed in claim 1 or 2, wherein the pH of the antibacterial phosphorus-free cleaning agent is 10-10.5.

6. The antibacterial phosphorus-free cleaning agent as claimed in claim 1 or 2, wherein the conductivity of the antibacterial phosphorus-free cleaning agent is 2-5 ms/cm.

7. The antibacterial phosphorus-free cleaning agent as claimed in claim 1 or 2, wherein the osmotic pressure of the antibacterial phosphorus-free cleaning agent is 600-800m0 sm/kg.

8. The use of the antibacterial phosphorus-free cleaning agent as defined in claim 1 or 2, which is used in a full-automatic biochemical analyzer.

Technical Field

The invention relates to the technical field of cleaning agents, in particular to an antibacterial phosphorus-free cleaning agent and a preparation method and application thereof.

Background

The full-automatic biochemical analyzer is an indispensable instrument and equipment for hospital clinical laboratory due to simple operation, reliable result and stable performance. The antibacterial phosphorus-free cleaning agent is also a necessity for daily operation of instruments, the consumption is high, and the antibacterial phosphorus-free cleaning agent in China mostly depends on import and is expensive, so that the medical cost is high. For example, Hitachi 7100, which is one of the most imported full-automatic biochemical analyzers, has the advantages of high detection speed, simple and convenient operation, good system repeatability, high accuracy and the like, the cleaning agent mainly adopts HITERGENT stock solution, HITERGENT stock solution is an imported cleaning agent, the price is higher, and although a small amount of domestic antibacterial phosphorus-free cleaning agent exists in the market at present, the actual effect is not ideal, and the requirements of customers cannot be met.

CN107881021A discloses a constant temperature bath cleaning solution and a preparation method thereof, wherein a bactericide of the constant temperature bath cleaning solution is Proclin 300, although Proclin 300 has low biological toxicity, the price is high, a surfactant is single TX-10, TX-10 only has single emulsifying capacity, and TX-10 is more in addition amount than TX-7, more viscous and not easy to weigh, triethanolamine basically crystallizes except in summer, is not easy to weigh, causes inconvenience for preparation of the cleaning solution, and is not beneficial to maintaining stability of cleaning solution performance.

Disclosure of Invention

The invention provides an antibacterial phosphorus-free cleaning agent, a preparation method and application thereof, which are suitable for a full-automatic biochemical analyzer.

The technical scheme of the invention is realized as follows: an antibacterial phosphorus-free cleaning agent, wherein each 1L of the antibacterial phosphorus-free cleaning agent contains: nonylphenol polyoxyethylene ether TX-710-20 ml, nonylphenol polyoxyethylene ether TX-1220-45 ml, monoethanolamine 10-17ml, boric acid 2-4g, absolute ethyl alcohol 10-30ml, toluene-4-sodium sulfonate 1-2g and the balance of deionized water.

Furthermore, each 1L of the antibacterial phosphorus-free cleaning agent contains: nonylphenol polyoxyethylene ether TX-715ml, nonylphenol polyoxyethylene ether TX-1230 ml, monoethanolamine 14ml, boric acid 3g, ethanol 20ml, toluene-4-sodium sulfonate 1.5g, and the balance of deionized water.

A preparation method of an antibacterial phosphorus-free cleaning agent comprises the following steps:

(1) adding monoethanolamine into deionized water to obtain a solution A, dissolving boric acid into absolute ethyl alcohol, uniformly mixing to obtain a solution B, adding the solution B into the solution A, and uniformly stirring to obtain a premixed solution;

(2) adding toluene-4-sodium sulfonate into the premixed solution obtained in the step (1), and uniformly stirring to obtain a mixed solution;

(3) and (3) adding nonylphenol polyoxyethylene ether TX-7 and nonylphenol polyoxyethylene ether TX-12 into the mixed solution obtained in the step (2), adding deionized water to a constant volume of 1L, uniformly stirring, and filtering to obtain the antibacterial phosphorus-free cleaning agent.

Further, in the step (2), a cellulose nitrate filter membrane with the pore size of 0.22um is adopted for filtration.

Further, the pH value of the antibacterial phosphorus-free cleaning agent is 10-10.5.

Further, the conductivity of the antibacterial phosphorus-free cleaning agent is 2-5 ms/cm.

Further, the osmotic pressure of the antibacterial non-phosphate cleaning agent is 600-800m0 sm/kg.

An application of antibacterial non-phosphorus detergent in full-automatic biochemical analyzer.

The invention has the beneficial effects that:

the antibacterial phosphorus-free cleaning agent is suitable for a full-automatic biochemical analyzer, is colorless and transparent liquid in appearance, has stable chemical properties, reduces the use cost while ensuring the test effect, saves the medical expenditure of people, is easy to popularize clinically, and has obvious social and economic benefits.

1. The invention adopts the toluene-4-sodium sulfonate as the solubilizer, reduces the viscosity and the turbidity of the cleaning agent, and has lower influence on the fluidity and the transparency of water after being added into the water bath.

2. The surfactant disclosed by the invention is a combination of TX-7 and TX-12, wherein TX-7 has excellent emulsification cleaning performance and stronger cleaning capability than other surfactants, but is diffused in water, and in addition, TX-12 is added to increase the water solubility of TX-7, and meanwhile, TX-12 is also a heavy-duty low-foaming lotion and can reduce the water hardness and increase the defoaming capability.

3. The monoethanolamine serving as the antirust agent can protect the sample sucking needle while washing the needle, and can provide an alkaline environment to wash off substances such as protein and the like on the sample sucking needle so as to prevent cross contamination; in addition, the monoethanolamine is liquid in most cases, the weighing is easy, and the monoethanolamine with the same concentration is dissolved in water, so that the PH value of the monoethanolamine is higher than that of triethanolamine, and the monoethanolamine is more favorable for corrosion prevention.

4. The preservative adopts boric acid to replace the common antibacterial agent sodium azide, has low toxicity and safe use, avoids the damage to reagent production personnel and environment, and can achieve the test effect equivalent to that of the antibacterial phosphorus-free cleaning agent in Hitachi original factories, but the cost is greatly reduced and the preparation method is simple.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive effort based on the embodiments of the present invention, are within the scope of the present invention.

An antibacterial phosphorus-free cleaning agent, wherein each 1L of the antibacterial phosphorus-free cleaning agent contains: nonylphenol polyoxyethylene ether TX-710-20 ml, nonylphenol polyoxyethylene ether TX-1220-45 ml, monoethanolamine 10-17ml, boric acid 2-4g, absolute ethyl alcohol 10-30ml, toluene-4-sodium sulfonate 1-2g and the balance of deionized water. The pH value of the antibacterial phosphorus-free cleaning agent is 10-10.5. The conductivity of the antibacterial phosphorus-free cleaning agent is 2-5 ms/cm. The osmotic pressure of the antibacterial non-phosphate cleaning agent is 600-.