阅读说明：本技术 一种智能啮齿类动物心脏灌流装置 (Intelligent rodent heart perfusion device ) 是由 魏盛 耿希文 李自发 张�浩 胡明会 于 2020-06-19 设计创作，主要内容包括：一种智能啮齿类动物心脏灌流装置,涉及动物实验装置技术领域,包括灌流室、解剖台、以及智能灌流器,智能灌流器内设有储液仓、转运仓、控制仓,所述的解剖台上设有用以固定实验动物的动物固定器,转运仓内设有抽液泵B,所述的抽液泵B的液体输入端与储液仓内的液体连通,液体输出端通过液体输送软管连接有心脏固定容器,心脏固定容器内还设有电磁血流传感器,控制仓内设有控制器,电磁血流传感器通过信号线与控制器连接,控制器通过电线与抽液泵B连接,控制器还电性连接有电源,智能灌流器与解剖台配合使用,解剖台设置于灌流室内。本发明操作简单,使用方便,可实现多通道灌流的智能化操作,降低了实验操作者的劳动强度,提高了灌流效率,保证了灌流质量。(The utility model provides an intelligence rodent heart perfusion device, relates to animal experiment device technical field, including perfusion chamber, dissection table and intelligent perfusion ware, be equipped with stock solution storehouse, transport storehouse, control storehouse in the intelligence perfusion ware, dissection table on be equipped with the animal fixer for fixed laboratory animal, be equipped with drawing liquid pump B in the transport storehouse, the liquid input of drawing liquid pump B and the liquid intercommunication in the stock solution storehouse, liquid output end has heart fixing container through liquid delivery hose connection, still is equipped with electromagnetic blood flow sensor in the heart fixing container, is equipped with the controller in the control storehouse, electromagnetic blood flow sensor passes through the signal line and is connected with the controller, the controller passes through the electric wire and is connected with drawing liquid pump B, controller still electric connection has the power, intelligent perfusion ware uses with the cooperation of dissection table, the dissection table sets up in the perfusion chamber. The invention has simple operation and convenient use, can realize the intelligent operation of multi-channel perfusion, reduces the labor intensity of experiment operators, improves the perfusion efficiency and ensures the perfusion quality.)

1. An intelligent rodent heart perfusion device characterized by: the intelligent perfusion device comprises a perfusion chamber, an dissecting table and an intelligent perfusion device, wherein the intelligent perfusion device comprises a shell, a liquid storage bin, a transfer bin and a control bin are arranged in the shell, an animal fixer for fixing experimental animals is arranged on the dissecting table, a liquid pump B is arranged in the transfer bin, a liquid input end of the liquid pump B is communicated with liquid in the liquid storage bin, a liquid output end of the liquid pump B is connected with a heart fixing container through a liquid conveying hose, the end part of the liquid conveying hose is connected with a perfusion needle, a sharp prick end of the perfusion needle penetrates through the outer wall of the heart fixing container and extends into the heart fixing container, an electromagnetic blood flow sensor is further arranged in the heart fixing container, a controller is arranged in the control bin, the electromagnetic blood flow sensor is connected with the controller through a signal line, and the controller is connected with the liquid pump B through an electric wire, the controller still electric connection have the power, intelligent perfusion ware and dissection table cooperation use, the dissection table set up in the perfusion room.

2. The intelligent rodent heart perfusion device of claim 1, wherein: a washing bottle for containing cleaning liquid, a buffer bottle for containing buffer liquid and a fixing bottle for containing fixing liquid are arranged in the liquid storage bin; the transfer bin is internally provided with 1 liquid pump A and a plurality of liquid pumps B, the bottoms of the flushing bottle, the buffer bottle and the fixed bottle are all provided with liquid outlets, the washing bottle is provided with 1 liquid outlet and is connected with the liquid input end of the liquid pump A through a liquid conveying pipe, the liquid output end of the liquid pump A is connected with a three-way joint, one end of the three-way joint is connected with the liquid pump A, the other two ends are respectively communicated with the buffer bottle and the fixed bottle through liquid conveying pipes which run through the top ends of the buffer bottle and the fixed bottle, the bottom of buffer bottle, fixed bottle be equipped with a plurality of liquid outlets, the liquid outlet of buffer bottle or fixed bottle be connected with the liquid input of drawing liquid pump B of difference through the liquid conveying pipe respectively, arbitrary the liquid outlet in all be equipped with the solenoid valve, the controller respectively with solenoid valve, drawing liquid pump A electric connection.

3. The intelligent rodent heart perfusion device of claim 2, wherein: the control cabin still be equipped with the touch-sensitive screen, the display opening of touch-sensitive screen in the preceding terminal surface of the casing at control cabin place still be equipped with switch button before on the terminal surface, the controller pass through the electric wire with the touch-sensitive screen and be connected with the signal line.

4. The intelligent rodent heart perfusion device of claim 3, wherein: the touch screen is also internally provided with an experiment control unit, the experiment control unit subdivides the operation modes according to the types, strains, ages in days and weights of experimental animals to classify the operation modes, the operation modes are set to be specific perfusion time and perfusion speed, the electromagnetic valve, the liquid pump A and the liquid pump B are controlled by the controller to be specifically implemented, and the acquisition modes of the perfusion time and the perfusion speed are as follows: selecting perfusion time and perfusion speed which can obtain optimal perfusion effect as perfusion operation modes of any small animal according to the result of multiple perfusion experiments of the small animal subdivided into the same subclass and data of relevant perfusion time and perfusion speed, wherein the experiment control unit displays a control interface on a touch screen, the control interface comprises a first interface and a second interface, the first interface is provided with type selection, strain selection, age-per-day input, weight input, a cleaning button and a perfusion button, the perfusion button is touched to enter the second interface, the second interface is provided with a channel selection button, and a start button, a pause button and a stop button are arranged above the channel selection button; the channel selection button is used for selecting perfusion operation of animal hearts in different heart fixing containers, and the controller controls different liquid pumps B and electromagnetic valves matched with the liquid pumps B to perfuse.

5. The intelligent rodent heart perfusion device of any one of claims 1-4, wherein: the heart fixing container is of a shell-like structure, the shell-like structure comprises a shell cover A and a shell cover B which are hinged with each other, inner cavities matched with the placing shapes of animal hearts are arranged in the shell cover A and the shell cover B, buckling pieces A which are matched with each other are respectively arranged at the edges of the shell cover A and the shell cover B and are connected in a buckling mode through the buckling pieces A, and overflow ports are also arranged at the edges of the shell cover A and the shell cover B; the puncture needle of the electromagnetic blood flow sensor is positioned in the inner mold cavity, the puncture needle, the perfusion needle and the inner mold cavity are matched with the animal heart placed in the inner mold cavity, when the animal heart is placed in the inner mold cavity and the heart fixing container is closed, the puncture needle of the electromagnetic blood flow sensor punctures the pericardium, and the perfusion needle punctures the left ventricle; the heart fixing containers are multiple, and inner mold cavities with different shapes and sizes are arranged to meet the fixing requirements of different animal hearts.

6. The intelligent rodent heart perfusion device of any one of claims 1-4, wherein: the animal fixer is arranged in the middle of the dissecting table and comprises a groove matched with the animal body and a chest clamp, one end of the chest clamp is hinged with the top of the groove wall on one side of the groove, the other end of the chest clamp is connected with the top of the groove wall on the other side of the groove through a fastener B in a buckling mode, and an operation opening is formed in the middle of the chest clamp.

7. The intelligent rodent heart perfusion device of claim 6, wherein: the table-board of the dissection table at the periphery of the groove is also provided with a drainage groove, the drainage groove surrounds the groove, the groove bottom of the drainage groove gradually inclines downwards to one end and extends, and a drainage tube is arranged at the lowest part of the groove bottom and is connected with a waste liquid collecting container through a connecting pipe.

8. The intelligent rodent heart perfusion device of claim 7, wherein: the top of perfusion chamber be equipped with transparent cage, be equipped with the light at the lower surface of cage, be equipped with the blow vent on the lateral wall of the both sides of perfusion chamber respectively, the outer wall of the one end of perfusion chamber on still be equipped with the gas vent, install the air discharge fan in gas vent department, the gas vent be located the outdoor one end of perfusion chamber and be connected with the formaldehyde purification ware through the breather pipe, the atmosphere is discharged to the gas after the purification.

9. The intelligent rodent heart perfusion device of claim 7, wherein: the connecting pipe is connected with a waste liquid collecting container through a waste liquid conveying hose, and the waste liquid collecting container is arranged outside the perfusion chamber; and a liquid leakage hole is formed at the bottom of the groove and is connected with the connecting pipe through a connecting pipe.

10. The intelligent rodent heart perfusion device of any one of claims 1-4 and 7-9, wherein: the controller is a control chip or a control circuit board or a PLC controller.

Technical Field

The invention relates to the technical field of animal experiment devices, in particular to an intelligent rodent heart perfusion device.

Background

In animal experiments, heart perfusion material drawing is an essential step, and perfusion results are closely related to the state of tissues. The heart perfusion is to expose the animal heart, insert the perfusion needle into the left ventricle, fix the perfusion needle, and perfuse the normal saline, pbs and paraformaldehyde sequentially. At present, no special animal heart perfusion device exists in the market, most experimenters adopt a flat-mouth needle head and a hose which are assembled by self, and the needle head is inserted into the heart and then clamps and closes the cardiac apex and the perfusion needle by using vascular clamps.

The traditional self-assembled perfusion device has the following defects:

1. the perfusion device and the surgical instrument are scattered, the assembly procedure is complex, and the preparation needs a long time before the operation;

2. must rely on the vascular pincers to fix, to the littleer small-size animal of heart, like mouse, young rat etc. usually need many times to try, can press from both sides tightly fixedly, not only the time-wasting, and the animal state also receives the influence, accurately pierces the left ventricle, pierces the depth and also hardly guarantees, and the perfusion success rate is lower.

3. The vascular clamp needs to clamp the apex of the heart and the perfusion needle, and the heart is easily damaged by slight struggle or shake of the animal, so that perfusion failure is caused.

4. The perfusion needs to use paraformaldehyde irritability great, and traditional flowing device does not have effective exhaust and recovery unit, and is very big to the human body injury.

5. The conventional perfusion apparatus lacks a corresponding dissecting table 20, and animal fixation and dissecting operations are complicated.

6. At present, a heart mould without a perfusion device is lack of a mould integrating fixation and automatic penetration, so that the experimental efficiency is low and the success rate of 100 percent cannot be guaranteed.

7. The traditional perfusion device judges the perfusion state only by observing the tissue color through naked eyes, cannot accurately master the state of a perfusion animal, is difficult to ensure the accuracy of an experimental result, and wastes buffer solution and fixing solution.

8. The traditional perfusion device still cannot effectively perform multi-channel experiments by using a peristaltic pump, the operation flow is complex, and the experiment efficiency is low.

Disclosure of Invention

To solve the problems of the prior art, the present invention provides an intelligent rodent heart perfusion device which systematically improves the disadvantages of the prior art.

In order to achieve the purpose, the technical scheme of the invention is as follows:

the utility model provides an intelligence rodent heart perfusion device, includes perfusion chamber, dissection table and intelligent perfusion ware, intelligent perfusion ware include the casing, the casing in be equipped with stock solution storehouse, transport storehouse, control bin, the dissection table on be equipped with the animal fixer that is used for fixed laboratory animal, the transport storehouse in be equipped with drawing liquid pump B, the liquid input of drawing liquid pump B and the liquid intercommunication in the stock solution storehouse, liquid output end has the heart fixed container through liquid delivery hose connection, liquid delivery hose's end connection have the perfusion needle, the point thorn end of perfusion needle pierce through the outer wall of heart fixed container and extend to the heart fixed container inside, the heart fixed container in still be equipped with electromagnetic blood flow sensor, the control bin in be equipped with the controller, electromagnetic blood flow sensor pass through the signal line and be connected with the controller, the controller is connected with the liquid pump B through an electric wire, the controller is also electrically connected with a power supply, the intelligent perfusion device is matched with an anatomical table for use, and the anatomical table is arranged in the perfusion chamber.

Preferably, a washing bottle for containing cleaning liquid, a buffer bottle for containing buffer liquid and a fixing bottle for containing fixing liquid are arranged in the liquid storage bin; the transfer bin is internally provided with 1 liquid pump A and a plurality of liquid pumps B, the bottoms of the flushing bottle, the buffer bottle and the fixed bottle are all provided with liquid outlets, the washing bottle is provided with 1 liquid outlet and is connected with the liquid input end of the liquid pump A through a liquid conveying pipe, the liquid output end of the liquid pump A is connected with a three-way joint, one end of the three-way joint is connected with the liquid pump A, the other two ends are respectively communicated with the buffer bottle and the fixed bottle through liquid conveying pipes which run through the top ends of the buffer bottle and the fixed bottle, the bottom of buffer bottle, fixed bottle be equipped with a plurality of liquid outlets, the liquid outlet of buffer bottle or fixed bottle be connected with the liquid input of drawing liquid pump B of difference through the liquid conveying pipe respectively, arbitrary the liquid outlet in all be equipped with the solenoid valve, the controller respectively with solenoid valve, drawing liquid pump A electric connection.

Preferably, the control cabin is further provided with a touch screen, a display opening of the touch screen is arranged on the front end face of the shell where the control cabin is located, a power switch button is further arranged on the front end face, and the controller is connected with the touch screen through an electric wire and a signal wire.

Preferably, the touch screen is further provided with an experiment control unit, the experiment control unit classifies operation modes by subdividing according to different types, strains, ages in days and weights of experimental animals, the operation modes are set to specific perfusion time and perfusion speed, and the controller controls the electromagnetic valve, the liquid drawing pump a and the liquid drawing pump B to implement the operation modes, and the acquisition modes of the perfusion time and the perfusion speed are as follows: selecting perfusion time and perfusion speed which can obtain optimal perfusion effect as perfusion operation modes of any small animal according to the result of multiple perfusion experiments of the small animal subdivided into the same subclass and data of relevant perfusion time and perfusion speed, wherein the experiment control unit displays a control interface on a touch screen, the control interface comprises a first interface and a second interface, the first interface is provided with type selection, strain selection, age-per-day input, weight input, a cleaning button and a perfusion button, the perfusion button is touched to enter the second interface, the second interface is provided with a channel selection button, and a start button, a pause button and a stop button are arranged above the channel selection button; the channel selection button is used for selecting perfusion operation of animal hearts in different heart fixing containers, and the controller controls different liquid pumps B and electromagnetic valves matched with the liquid pumps B to perfuse.

Preferably, the heart fixing container is of a shell-like structure, the shell-like structure comprises a shell cover A and a shell cover B which are hinged with each other, inner cavities matched with the placing shapes of the hearts of the animals are arranged in the shell cover A and the shell cover B, clamping pieces A matched with each other are respectively arranged at the edges of the shell cover A and the shell cover B and are connected in a clamping mode through the clamping pieces A, and overflow ports are also arranged at the edges of the shell cover A and the shell cover B; the puncture needle of the electromagnetic blood flow sensor is positioned in the inner mold cavity, the puncture needle, the perfusion needle and the inner mold cavity are matched with the animal heart placed in the inner mold cavity, when the animal heart is placed in the inner mold cavity and the heart fixing container is closed, the puncture needle of the electromagnetic blood flow sensor punctures the pericardium, and the perfusion needle punctures the left ventricle; the heart fixing containers are multiple, and inner mold cavities with different shapes and sizes are arranged to meet the fixing requirements of different animal hearts.

Preferably, the animal fixer set up in the middle part of dissection table, including the recess that matches with the animal size and thorax press from both sides, the thorax press from both sides one end and the cell wall top of one side of recess articulated, the other end passes through fastener B and is connected with the cell wall top buckle of recess opposite side, is equipped with the operation mouth in the middle part of thorax press from both sides.

Preferably, the table-board of the dissection table at the periphery place of recess on still be equipped with the drainage groove, the drainage groove surround in the recess, and the tank bottom of drainage groove is to the gradual downward sloping extension of one end to be equipped with the drainage tube in the lowest department of tank bottom, the drainage tube be connected with waste liquid collection container through the connecting pipe.

Preferably, the top of perfusion chamber be equipped with transparent cage, be equipped with the light at the lower surface of cage, be equipped with the blow vent on the lateral wall of the both sides of perfusion chamber respectively, the outer wall of the one end of perfusion chamber on still be equipped with the gas vent, install the air discharge fan in gas vent department, the gas vent be located the outdoor one end of perfusion chamber and be connected with the formaldehyde purification ware through the breather pipe, the atmosphere is discharged to the gas after purifying.

Preferably, the connecting pipe is connected with a waste liquid collecting container through a waste liquid conveying hose, and the waste liquid collecting container is arranged outside the perfusion chamber; and a liquid leakage hole is formed at the bottom of the groove and is connected with the connecting pipe through a connecting pipe.

Preferably, the controller is a control chip or a control circuit board or a PLC controller.

The intelligent rodent heart perfusion device has the following beneficial effects:

1. the perfusion device is simple to operate and convenient to use, and perfusion can be easily performed at the optimal perfusion time and perfusion speed through the interface button on the touch screen, so that the labor intensity of an experiment operator is greatly improved, the perfusion efficiency is improved, and the experiment quality is ensured;

2. the invention realizes the unified control of the multi-channel perfusion operation and can perform the perfusion operation on a plurality of animal hearts at the same time;

3. the heart fixing container is provided with the inner cavity adaptive to the animal heart, so that the puncture needle and the perfusion needle of the electromagnetic blood flow sensor can be respectively punctured into corresponding parts of the heart while the heart fixing container is closed, the difficulty of heart fixing operation is greatly reduced, and the experimental efficiency and the success rate are improved;

4. by arranging the drainage groove, the drainage tube, the leakage hole, the connecting tube and the waste liquid collecting container, residual liquid of blood, buffer solution, flushing fluid and stationary liquid in the experimental process can be smoothly collected into the waste liquid collecting container, so that the cleanness and the sanitation of the dissecting table are facilitated;

5. the invention can quickly discharge the air containing formaldehyde in the perfusion chamber by arranging the exhaust fan, the vent pipe and the formaldehyde purifier, and the air is discharged into the atmosphere after being purified, thereby ensuring the environmental protection and protecting the health of experiment operators.

6. The optimal perfusion time and the optimal perfusion speed related by the invention are obtained based on a large number of perfusion experiments of animals of the same subclass, and the optimal perfusion time and the optimal perfusion speed are arranged in the perfusion process of the intelligent perfusion device through the controller, so that perfusion can be carried out in an optimal perfusion mode under the simplest operation.

Drawings

FIG. 1: the invention discloses a schematic view of a top-down structure of an intelligent perfusion device;

FIG. 2: the invention discloses a schematic side view structure diagram of an intelligent perfusion device;

FIG. 3: the invention discloses a front view structure schematic diagram of an intelligent perfusion device;

FIG. 4: the invention discloses a top view of the matching relationship between a perfusion chamber and an dissecting table;

FIG. 5: the invention discloses a structural schematic diagram of a heart fixing container;

FIG. 6: a first interface schematic of the present invention;

FIG. 7: a second interface schematic of the invention;

1: housing, 1-1: a liquid storage bin, 1-2: a transfer bin, 1-3: control cabin, 2: touch screen, 3: power switch button, 4: rinse bottle, 5: buffer bottle, 6: fixed bottle, 7: liquid pump a, 8: liquid pump B, 9: liquid delivery pipe, 10: three-way connection, 11: heart fixation vessel, 11-1: case cover A, 11-2: case cover B, 11-3: inner cavity, 11-4: electromagnetic blood flow sensor, 11-5: perfusion needle, 11-6: an overflow port, 11-7: a hinged shaft, 11-8: fastener a, 12: liquid outlet, 13: controller, 14: signal line, 15: electric wire, 16: delivery pipe, 17: cage, 18: vent, 19: illumination lamp, 20: dissecting table, 20-1: groove, 20-2: chest clip, 20-3: drainage groove, 20-4: fastener B, 20-5: drainage tube, 20-6: surgical operation port, 21: connecting pipe, 22: waste liquid conveying hose, 23: waste liquid collection container, 24: breather pipe, 25: formaldehyde purifier, 26: an exhaust fan.

Detailed Description

In the following, embodiments of the present invention are described in detail in a stepwise manner, which is merely a preferred embodiment of the present invention and is not intended to limit the scope of the present invention, and any modifications, equivalents, improvements, etc. made within the spirit and principle of the present invention should be included in the scope of the present invention.