阅读说明：本技术 一种脐带标本的保存方法 (Preservation method of umbilical cord specimen ) 是由 魏伟 嵐山芮 许超 于 2020-06-29 设计创作，主要内容包括：本发明公开了一种脐带标本的保存方法。本发明首次提出了硝酸甘油在脐带标本保存中的应用。本发明的脐带标本保存方法可以实现在常温下保存,大大降低了对库存、运输、交接条件的要求,可节约成本；同时能有效抑制细菌污染,维持脐带中间充质干细胞的生物活性,可为临床和基础研究提供高质量的生物资源。(The invention discloses a preservation method of an umbilical cord specimen. The invention provides the application of nitroglycerin in the preservation of an umbilical cord specimen for the first time. The umbilical cord specimen preservation method can realize preservation at normal temperature, greatly reduces the requirements on the conditions of inventory, transportation and handover, and can save the cost; meanwhile, the method can effectively inhibit bacterial pollution, maintain the biological activity of the mesenchymal stem cells in the umbilical cord, and provide high-quality biological resources for clinical and basic research.)

1. Application of nitroglycerin in preservation of umbilical cord specimens.

2. A preservation method of an umbilical cord specimen, which is characterized by comprising the following steps: after the fetus is delivered, the umbilical cord is collected, disinfected by alcohol, cleaned, put into a container filled with a mixed solution of nitroglycerin and normal saline, sealed, and stored at the temperature of 2-15 ℃ until the fetus reaches a specified destination.

3. The method for preserving an umbilical cord specimen according to claim 2, wherein:

the ratio of nitroglycerin to normal saline is (volume ratio) 0.2-2: 100.

4. the method for preserving an umbilical cord specimen according to claim 2, wherein:

the ratio of nitroglycerin to normal saline is 1: 100.

5. the method for preserving an umbilical cord specimen according to claim 2, wherein:

the normal saline is 0.9% sodium chloride aqueous solution.

6. The method for preserving an umbilical cord specimen according to claim 2, wherein:

the alcohol disinfection time is 0.5-2 minutes.

7. The method for preserving an umbilical cord specimen according to claim 2, wherein:

the alcohol disinfection time is 1 minute.

8. The method for preserving an umbilical cord specimen according to claim 2, wherein:

the reagent used for cleaning is normal saline.

9. The method for preserving an umbilical cord specimen according to claim 2, wherein:

the storage time is less than 24 hours.

10. The method for preserving an umbilical cord specimen according to claim 2, wherein:

the time for collecting the umbilical cord is within 30 seconds after the delivery of the fetus.

Technical Field

The invention belongs to the technical field of biological specimen collection and preservation, and particularly relates to a preservation method of an umbilical cord specimen.

Background

The umbilical cord is a delivery waste tissue, the mesenchymal stem cells in the umbilical cord are rich, traumatic puncture is not needed, the expansion capacity of the umbilical cord is higher than that of the mesenchymal stem cells from bone marrow, and the umbilical cord becomes one of the important sources of the recent research on the mesenchymal stem cells. However, in the work of the daily umbilical cord stem cell bank, because the labor way and the collection method of medical staff are improper, the umbilical cord specimen is easily polluted by bacteria, and the waste of precious resources is caused.

In order to avoid pollution, penicillin and streptomycin double-antibody are required to be added into the collection liquid. However, the double antibody is sensitive to temperature, needs to be stored at-20 ℃ in a freezing way, needs to be placed in a cold storage layer for short-term storage, is inconvenient to operate, and is easy to degrade components, so that the bacteriostatic effect is influenced. Therefore, there is a need to develop a simple and effective method for collecting and preserving umbilical cord specimen.

Nitroglycerin (C)₃H₅N₃O₉Glyceryl trinitrate) is colorless or yellow oily transparent liquid with the chemical formula and the structural formulaIs mainly used for treating and preventing angina pectoris, is used as a vasodilator, and can be used for treating congestive heart failure.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention mainly aims to provide the application of nitroglycerin in the preservation of the umbilical cord specimen.

Another object of the present invention is to provide a method for preserving an umbilical cord specimen.

The purpose of the invention is realized by the following technical scheme:

application of nitroglycerin in preservation of umbilical cord specimens.

A method for preserving umbilical cord specimen includes collecting umbilical cord after delivery of fetus, disinfecting with alcohol, washing, putting it in a container containing the mixture of nitroglycerin and physiological saline, sealing, and preserving at 2-15 deg.C until the umbilical cord specimen is transported to a specified destination.

The time for collecting the umbilical cord is preferably within 30 seconds after delivery of the fetus.

The preferred ratio of nitroglycerin to normal saline is 0.2-2: 100, respectively; more preferably 1: 100.

the normal saline is medical sodium chloride injection, namely 0.9% sodium chloride aqueous solution.

The alcohol disinfection time is preferably 0.5-2 minutes; more preferably 1 minute.

The reagent for washing is preferably physiological saline.

The storage time is preferably less than 24 hours; more preferably less than 19 hours.

Compared with the prior art, the invention has the following advantages and effects:

the invention provides the application of nitroglycerin in the preservation of an umbilical cord specimen for the first time. The umbilical cord specimen preservation method can realize preservation at normal temperature, greatly reduces the requirements on the conditions of inventory, transportation and handover, and can save the cost; meanwhile, the method can effectively inhibit bacterial pollution, maintain the biological activity of the mesenchymal stem cells in the umbilical cord, and provide high-quality biological resources for clinical and basic research.

Drawings

FIG. 1 is a schematic diagram showing the state of cells at day 2 of culture after passaging.

FIG. 2 is a diagram showing the results of flow cytometry.

Detailed Description

The present invention will be described in further detail with reference to examples and drawings, but the present invention is not limited thereto. The experimental methods used in the present invention are all conventional methods unless otherwise specified, and the raw materials and auxiliary agents used therein are all commercially available raw materials and auxiliary agents available from conventional markets, etc., unless otherwise specified.