阅读说明：本技术 人工结香沉香、沉香精油富集液、沉香香皂及其生产方法 (Artificial aquilaria wood, aquilaria wood essential oil enrichment liquid, aquilaria wood soap and production method thereof ) 是由 黎妹 于 2020-06-05 设计创作，主要内容包括：本发明涉及公开了一种人工结香沉香、沉香精油富集液、沉香香皂及其生产方法。本发明采用化学刺激-接菌法获得沉香人工结香部位,通过超临界CO<Sub>2</Sub>技术和分子蒸馏技术得到富集倍半萜类和色酮类化合物有效成分的沉香精油富集液,加入银耳提取液、皂基(如分馏椰子油、蓖麻油、橄榄油、氢氧化钠)、品质调理剂、pH调理剂,得到一种具有清洁滋润、杀菌消炎、镇静安神功能的沉香香皂。本发明制备过程不产生难以降解的废料、废液,对环境无影响,产品呈弱酸性,对皮肤安全无刺激,持久留香,相比传统沉香对真菌、白色念珠菌、大肠杆菌、金黄色葡萄球菌具有更好的杀灭效果。(The invention relates to an artificial agilawood, agilawood essential oil enrichment liquid, agilawood soap and a production method thereof. The invention adopts a chemical stimulation-inoculation method to obtain the artificial agilawood position, and supercritical CO is adopted to carry out 2 The method comprises obtaining concentrated solution of lignum Aquilariae Resinatum essential oil rich in sesquiterpene and chromone compounds, adding Tremella extractive solution, soap base (such as fractionated coconut oil, oleum ricini, oleum Olivarum, and sodium hydroxide), quality conditioner, and pH conditioner to obtain lignum Aquilariae Resinatum soap with cleaning, moistening, antibacterial, antiinflammatory, tranquilizing, and tranquilizing effects. The preparation process of the invention does not produce waste materials and waste liquids which are difficult to degrade, has no influence on the environment, the product is faintly acid, is safe and non-irritant to skin, and can keep fragrance for a long time, compared with the traditional agilawood, the invention is true to trueThe bacteria, candida albicans, escherichia coli and staphylococcus aureus have better killing effect.)

1. The production method of the artificial edgeworthia chrysantha is characterized by comprising the following steps:

(1) selecting 5-10 years old eaglewood trees with the diameter larger than 10 CM;

(2) drilling holes on the same side of a trunk, drilling a small hole which is inclined downwards at intervals of 40-60CM from top to bottom in the upwind direction by using a drilling tool, wherein the hole diameter is 1-3CM, and the drilling depth is 1/4-1/3 of the diameter of the aquilaria sinensis tree;

3) 100-300g of 1-3% formic acid bacterial liquid is injected into the drilled hole, the drilled hole is sealed by a plastic film, and the time for collecting the agilawood blocks is more than 6 months, wherein the formic acid bacterial liquid contains one or more than two of staphylococcus aureus, multi-resistant staphylococcus aureus, candida albicans or escherichia coli.

2. The method for producing artificial edgeworthia chrysantha according to claim 1, characterized in that: the preparation method of the formic acid bacterial liquid comprises the following steps: respectively preparing bacterial suspensions of staphylococcus aureus, multi-resistant staphylococcus aureus, candida albicans or escherichia coli with the density of 5x10⁶CFU/ml; one or more than two bacterial suspensions are taken, 1-4 parts of physiological saline is added to form a mixed solution in a five-fold amount, and finally, formic acid is added to prepare formic acid bacterial solution with the mass percentage of formic acid of 1-3%.

3. The method for producing artificial edgeworthia chrysantha according to claim 1, characterized in that: the culture medium of the staphylococcus aureus is tryptone soy broth culture medium TSB (TSB culture medium) 37Culturing at 24 hr, diluting with physiological saline to 5 × 10 deg.C⁶Bacterial suspension at CFU/ml density; the culture medium of multi-resistant Staphylococcus aureus is tryptone soy broth TSB culture medium, and is cultured at 37 deg.C for 24 hr, diluted with normal saline to 5 × 10⁶Bacterial suspension at CFU/ml density; the Candida albicans culture medium is potato glucose agar PDA culture medium, and is cultured at 25 deg.C for 24 hr, diluted with normal saline, and made into 5 × 10⁶Bacterial suspension at CFU/ml density; the Escherichia coli culture medium is conventional LB culture medium, cultured at 37 deg.C for 24 hr, diluted with normal saline to 5 × 10⁶Bacterial suspension at CFU/ml density.

4. The artificial aquilaria wood obtained by the production method of claim 1, 2 or 3.

5. The production method of the agilawood essential oil enrichment solution is characterized by comprising the following steps of:

(1) extracting the wood blocks of the artificial lignum Aquilariae Resinatum of claim 4 by pulverizing into 40-80 mesh granules, and extracting with supercritical CO₂The equipment is used for extracting lignum Aquilariae Resinatum granules at 40-65 deg.C under 20-35MPa and CO₂The flow rate is 100-300L/h, the extraction time is set to be 90-240 minutes, and the agilawood extract is obtained;

(2) purifying and enriching the agilawood extract obtained in the step (1) by using molecular distillation equipment, setting the temperature of a water bath at 75-80 ℃, the vacuum degree of a vacuum system at 0.1-100Pa, the first-stage purification temperature at 80-100 ℃, the first-stage film scraping speed at 150-ion of 250r/min, the first-stage collection temperature at 10-50 ℃, the second-stage purification temperature at 100-ion of 130 ℃, the second-stage film scraping speed at 150-ion of 250r/min and the second-stage collection temperature at 10-50 ℃ to obtain the second-stage light component agilawood essential oil enriched liquid.

6. The aquilaria essential oil enrichment solution prepared by the production method of claim 5.

7. An agilawood soap is characterized in that: the formula comprises the following components in percentage by weight:

8. the agilawood soap of claim 7, wherein: the tremella extract is prepared by the following steps:

s1, crushing the dried tremella, and sieving the crushed tremella with a 60-100-mesh sieve for later use;

s2, pre-soaking and weighing tremella powder into a container, and adding 30-60 times of deionized water to soak for 4-6 hours to obtain a tremella solution;

s3, ultrasonic extraction, namely placing the container filled with the soaked white fungus solution into ultrasonic extraction equipment, setting the parameters of the ultrasonic equipment to be 300-500W, and extracting for 20-60 minutes to obtain the white fungus extraction solution;

s4, centrifuging to transfer the S3 white fungus extraction solution into a centrifuge, setting the speed as 2000-4000r/min and the time as 3-15 minutes, and obtaining the supernatant which is the white fungus extraction solution.

9. The agilawood soap of claim 7, wherein: the soap base is prepared from the following components in parts by weight of 1-4: 3:2 coconut oil, castor oil, olive oil and sodium hydroxide are prepared by saponification reaction; the quality conditioner is one or more than two of EDTA, vitamin E and glycerol. The pH conditioning agent is one or more than two of vitamin C, citric acid and L-lactic acid, and the addition amount of the pH conditioning agent is to adjust the pH value range of the soap base to 5-6.5.

10. The method for producing the agilawood soap according to claim 7, 8 or 9, characterized by comprising the steps of:

(1) kneading and weighing the soap base, the agilawood essential oil enrichment liquid, the tremella extract and the quality conditioner according to a ratio, adding the weighed materials into a conventional soap making kneader, kneading and stirring uniformly for 15-30 minutes at the stirring temperature of 30-40 ℃;

(2) grinding, namely adding the kneaded and uniformly stirred material into a grinder, wherein the thickness of the ground soap flakes is 0.2-0.4 mm;

(3) putting the ground soap flakes into a vacuum plodder by a vacuum layering machine, and performing vacuum layering, wherein the temperature of a plodder muzzle is 40 ℃, and the temperature of cooling water introduced into the plodder is 3-10 ℃;

(4) printing, namely putting the pressed strip soap body into a printer for printing, and controlling the temperature of a mould to be between-10 and-25 ℃ to obtain the artificial agilawood scented soap.

Technical Field

The invention relates to the field of plant planting, deep processing and daily chemicals, in particular to an artificial aquilaria wood, an aquilaria wood essential oil enrichment liquid, an aquilaria wood soap and a production method thereof.

Background

Aquilaria sinensis refers to a piece of lignum Aquilariae Resinatum which is a special resin produced by self-repairing of Aquilaria sinensis (lour.) Spreng of Thymelaeaceae after natural or artificial injury. Chenxiang can be used as a medicine for promoting qi circulation to relieve pain, warming middle energizer to arrest vomiting, and absorbing qi to relieve dyspnea. It is commonly used for chest and abdomen distention, stuffy pain, stomach cold, vomiting, hiccup, deficiency of the kidney, dyspnea with rapid and urgent respiration. The lignum Aquilariae Resinatum can be used as spice to enhance fragrance by burning, smearing or fumigating, and has effects of tranquilizing mind, relieving pressure, resisting bacteria and diminishing inflammation.

The natural generation of the agilawood needs decades or even decades, and the time is very long. Meanwhile, the agilawood is deficient in natural resources, and the wild aquilaria sinensis is almost eliminated. Therefore, the government encourages the farmers to plant agilawood trees and obtains agilawood by the artificial agilawood formation technology. At present, no unified agilawood producing technical standard exists in China, and agilawood quality evaluation indexes obtained by each farmer are different.

Perfumed soap is a washing product made up by using oil, alkali and adjuvant, and can be used for cleaning and maintaining skin, hair and clothes. With the improvement of living standard of people, more functions of the soap are developed, and besides the basic cleaning function, the soap with other functions such as sterilization, whitening, moistening, mosquito repelling and the like is also popular with people. People in the new era advocate 'nature, safety, green and efficiency', enrich the functionality of the soap, and pay attention to the effectiveness and safety of soap products.

In recent years, the agilawood soap prepared from the artificial agilawood is easy to crack and paste, has short fragrance retention time, dry and rough skin after use, skin allergy after use and the like, and is related to the quality safety and the subsequent production process of the artificial agilawood.

Disclosure of Invention

In order to solve the existing problems, the invention aims to provide the artificial aquilaria sinensis and the production method thereof, and the prepared artificial aquilaria sinensis has stronger sterilization capability.

The invention also aims to provide the agilawood essential oil enrichment solution and a production method thereof.

The invention also aims to provide the artificial heartleaf eaglewood soap and the production method thereof, wherein the artificial heartleaf eaglewood soap has the functions of cleaning and moistening, sterilizing and diminishing inflammation, and tranquilizing and allaying excitement, and is not easy to crack and paste.

The purpose of the invention is realized as follows: the production method of the artificial edgeworthia chrysantha is characterized by comprising the following steps:

(1) selecting 5-10 years old eaglewood trees with the diameter larger than 10 CM;

(2) drilling holes on the same side of a trunk, drilling a small hole which is inclined downwards at intervals of 40-60CM from top to bottom in the upwind direction by using a drilling tool, wherein the hole diameter is 1-3CM, and the drilling depth is 1/4-1/3 of the diameter of the aquilaria sinensis tree;

3) 100-300g of 1-3% formic acid bacterial liquid is injected into the drilled hole, the drilled hole is sealed by a plastic film, and the time for collecting the agilawood blocks is more than 6 months, wherein the formic acid bacterial liquid contains one or more than two of staphylococcus aureus, multi-resistant staphylococcus aureus, candida albicans or escherichia coli.

Preferably, the preparation method of the formic acid bacteria liquid comprises the following steps: respectively preparing bacterial suspensions of staphylococcus aureus, multi-resistant staphylococcus aureus, candida albicans or escherichia coli with the density of 5x10⁶CFU/ml; one or more than two bacterial suspensions are taken, 1-4 parts of physiological saline is added to form a mixed solution in a five-fold amount, and finally, formic acid is added to prepare formic acid bacterial solution with the mass percentage of formic acid of 1-3%.

The culture medium of Staphylococcus aureus is tryptone soy broth TSB culture medium, and is cultured at 37 deg.C for 24 hr, diluted with normal saline, and formulated into 5X10⁶Bacterial suspension at CFU/ml density; the culture medium of multi-resistant Staphylococcus aureus is tryptone soy broth TSB culture medium, and is cultured at 37 deg.C for 24 hr, diluted with normal saline to 5 × 10⁶Bacterial suspension at CFU/ml density; the Candida albicans culture medium is potato glucose agar PDA culture medium, and is cultured at 25 deg.C for 24 hr, diluted with normal saline, and made into 5 × 10⁶Bacterial suspension at CFU/ml density; the Escherichia coli culture medium is conventional LB culture medium, cultured at 37 deg.C for 24 hr, diluted with normal saline to 5 × 10⁶Bacterial suspension at CFU/ml density.

The artificial lignum Aquilariae Resinatum obtained by the above production method.

The production method of the agilawood essential oil enrichment solution is characterized by comprising the following steps of:

(1) extracting by pulverizing the above artificial lignum Aquilariae Resinatum into 40-80 mesh granules, and supercritical CO₂Granule of equipment pair eaglewoodExtracting the granules at 40-65 deg.C under 20-35MPa with CO₂The flow rate is 100-300L/h, the extraction time is set to be 90-240 minutes, and the agilawood extract is obtained;

(2) purifying and enriching the agilawood extract obtained in the step (1) by using molecular distillation equipment, setting the temperature of a water bath at 75-80 ℃, the vacuum degree of a vacuum system at 0.1-100Pa, the first-stage purification temperature at 80-100 ℃, the first-stage film scraping speed at 150-ion of 250r/min, the first-stage collection temperature at 10-50 ℃, the second-stage purification temperature at 100-ion of 130 ℃, the second-stage film scraping speed at 150-ion of 250r/min and the second-stage collection temperature at 10-50 ℃ to obtain the second-stage light component agilawood essential oil enriched liquid.

The agilawood essential oil enrichment solution prepared by the preparation method.

An agilawood soap is characterized in that: the formula comprises the following components in percentage by weight:

the tremella extract is prepared by the following steps:

s1, crushing the dried tremella, and sieving the crushed tremella with a 60-100-mesh sieve for later use;

s2, pre-soaking and weighing tremella powder into a container, and adding 30-60 times of deionized water to soak for 4-6 hours to obtain a tremella solution;

s3, ultrasonic extraction, namely placing the container filled with the soaked white fungus solution into ultrasonic extraction equipment, setting the parameters of the ultrasonic equipment to be 300-500W, and extracting for 20-60 minutes to obtain the white fungus extraction solution;

s4, centrifuging to transfer the S3 white fungus extraction solution into a centrifuge, setting the speed as 2000-4000r/min and the time as 3-15 minutes, and obtaining the supernatant which is the white fungus extraction solution.

The soap base is prepared from the following components in parts by weight of 1-4: 3:2 coconut oil, castor oil, olive oil and sodium hydroxide are prepared by saponification reaction; the quality conditioner is one or more than two of EDTA, vitamin E and glycerol. The pH conditioning agent is one or more than two of vitamin C, citric acid and L-lactic acid, and the addition amount of the pH conditioning agent is to adjust the pH value range of the soap base to 5-6.5.

The production method of the agilawood soap is characterized by comprising the following steps:

(1) kneading and weighing the soap base, the agilawood essential oil enrichment liquid, the tremella extract and the quality conditioner according to a ratio, adding the weighed materials into a conventional soap making kneader, kneading and stirring uniformly for 15-30 minutes at the stirring temperature of 30-40 ℃;

(2) grinding, namely adding the kneaded and uniformly stirred material into a grinder, wherein the thickness of the ground soap flakes is 0.2-0.4 mm;

(3) putting the ground soap flakes into a vacuum plodder by a vacuum layering machine, and performing vacuum layering, wherein the temperature of a plodder muzzle is 40 ℃, and the temperature of cooling water introduced into the plodder is 3-10 ℃;

(4) printing, namely putting the pressed strip soap body into a printer for printing, and controlling the temperature of a mould to be between-10 and-25 ℃ to obtain the artificial agilawood scented soap.

The invention adopts a chemical stimulation-inoculation method to obtain the artificial agilawood position, and supercritical CO is adopted to carry out₂The method comprises obtaining concentrated solution of lignum Aquilariae Resinatum essential oil rich in sesquiterpene and chromone compounds, adding Tremella extractive solution, soap base (such as fractionated coconut oil, oleum ricini, oleum Olivarum, and sodium hydroxide), quality conditioner, and pH conditioner to obtain lignum Aquilariae Resinatum soap with cleaning, moistening, antibacterial, antiinflammatory, tranquilizing, and tranquilizing effects. The preparation process of the invention does not produce waste materials and waste liquids which are difficult to degrade, has no influence on the environment, the product is faintly acid, is safe and non-irritant to skin, and can keep fragrance for a long time, and compared with the traditional agilawood, the invention has better killing effect on fungi, candida albicans, escherichia coli and staphylococcus aureus.

Detailed Description

The invention relates to a production method of artificial agilawood, which comprises the following steps:

(1) selecting 5-10 years old eaglewood trees with the diameter larger than 10 CM;

(2) drilling holes on the same side of a trunk, drilling a small hole which is inclined downwards at intervals of 40-60CM from top to bottom in the upwind direction by using a drilling tool, wherein the hole diameter is 1-3CM, and the drilling depth is 1/4-1/3 of the diameter of the aquilaria sinensis tree;

3) 100-300g of formic acid bacterial liquid diluted by normal saline and 1-3 percent is injected into the drilled hole, the drilled hole is sealed by a plastic film, and the time for collecting the lignum aquilariae resinatum wood block is more than 6 months, wherein the formic acid bacterial liquid contains one or more than two of staphylococcus aureus, multi-resistant staphylococcus aureus, candida albicans or escherichia coli.

The time for collecting the lignum Aquilariae Resinatum blocks is more than 6 months, preferably more than 10 months, and generally 10-12 months in view of production efficiency, at this time, the lignum Aquilariae Resinatum blocks have good quality.

Preferably, the preparation method of the formic acid bacteria liquid comprises the following steps: preferably, the preparation method of the formic acid bacteria liquid comprises the following steps: respectively preparing bacterial suspensions of staphylococcus aureus, multi-resistant staphylococcus aureus, candida albicans or escherichia coli with the density of 5x10⁶CFU/ml; one or more than two bacterial suspensions are taken, 1-4 parts of physiological saline is added to form a mixed solution in a five-fold amount, and finally, formic acid is added to prepare formic acid bacterial solution with the mass percentage of formic acid of 1-3%.

Wherein the culture medium of Staphylococcus aureus is tryptone soy broth TSB culture medium, and is prepared by culturing at 37 deg.C for 24 hr, diluting with normal saline to 5 × 10⁶Bacterial suspension at CFU/ml density; the culture medium of multi-resistant Staphylococcus aureus is tryptone soy broth TSB culture medium, and is cultured at 37 deg.C for 24 hr, diluted with normal saline to 5 × 10⁶Bacterial suspension at CFU/ml density; the Candida albicans culture medium is potato glucose agar PDA culture medium, and is cultured at 25 deg.C for 24 hr, diluted with normal saline, and made into 5 × 10⁶Bacterial suspension at CFU/ml density; the Escherichia coli culture medium is conventional LB culture medium, cultured at 37 deg.C for 24 hr, diluted with normal saline to 5 × 10⁶Bacterial suspension at CFU/ml density.

The artificial lignum Aquilariae Resinatum obtained by the above production method.

A production method of agilawood essential oil enrichment liquid comprises the following steps:

(1) extracting by pulverizing the above artificial lignum Aquilariae Resinatum into granules of 40-80 mesh, and supercritical CO₂The equipment is used for extracting lignum Aquilariae Resinatum granules at 40-65 deg.C under 20-35MPa and CO₂The flow rate is 100-300L/h, the extraction time is set to be 90-240 minutes, and the agilawood extract is obtained;

(2) purifying and enriching the agilawood extract obtained in the step (1) by using molecular distillation equipment, setting the temperature of a water bath at 75-80 ℃, the vacuum degree of a vacuum system at 0.1-100Pa, the first-stage purification temperature at 80-100 ℃, the first-stage film scraping speed at 150-ion of 250r/min, the first-stage collection temperature at 10-50 ℃, the second-stage purification temperature at 100-ion of 130 ℃, the second-stage film scraping speed at 150-ion of 250r/min and the second-stage collection temperature at 10-50 ℃ to obtain the second-stage light component agilawood essential oil enriched liquid.

The agilawood essential oil enrichment solution prepared by the preparation method.

An agilawood soap is characterized in that: the formula comprises the following components in percentage by weight:

preferably, the tremella extract is prepared by the following steps:

s1, crushing the dried tremella, and sieving the crushed tremella with a 60-100-mesh sieve for later use;

s2, pre-soaking and weighing tremella powder into a container, and adding 30-60 times of deionized water to soak for 4-6 hours to obtain a tremella solution;

s3, ultrasonic extraction, namely placing the container filled with the soaked white fungus solution into ultrasonic extraction equipment, setting the parameters of the ultrasonic equipment to be 300-500W, and extracting for 20-60 minutes to obtain the white fungus extraction solution;

s4, centrifuging to transfer the S3 white fungus extraction solution into a centrifuge, setting the speed as 2000-4000r/min and the time as 3-15 minutes, and obtaining the supernatant which is the white fungus extraction solution.

Preferably, the soap base is prepared from the following components in a weight ratio of 1-4: 3:2 coconut oil, castor oil, olive oil and sodium hydroxide are prepared by saponification reaction. Preferably, the soap base is prepared by the following steps:

s1, respectively weighing the fractionated coconut oil, the castor oil and the olive oil according to the proportion, adding the materials into a heat-resistant container, heating the materials to 30-40 ℃ in a water bath heating mode, and uniformly stirring the materials;

s2, weighing deionized water 2.5-3.5 times (most preferably 3 times) the amount of sodium hydroxide, adding into an alkali-resistant glass container, slowly adding the calculated required sodium hydroxide with rapid stirring, and cooling to 35-40 ℃ after the sodium hydroxide is completely dissolved to obtain sodium hydroxide alkali liquor for later use;

the mass (m) of sodium hydroxide is calculated as follows:

the proportion of the fractionated coconut oil, the castor oil and the olive oil is A to B to C; the saponification values of fractionated coconut oil, castor oil and olive oil are 0.190, 0.129 and 0.134 respectively; the total mass of the fractionated coconut oil, the castor oil and the olive oil is M after mixing.

Sodium hydroxide mass (M) { a/(a + B + C) × 0.190+ B/(a + B + C) × 0.129+ C/(a + B + C) × 0.134} × M

S3, slowly adding the cooled alkali liquor of S2 into an S1 container with stirring, stirring for 30-40 minutes, completely saponifying the alkali liquor and the mixed oil of S1, and adding a pH conditioner to adjust the pH value to 5-6.5 to obtain the genuine soap.

Preferably, the quality conditioner is one or more than two selected from EDTA, vitamin E and glycerol; it is preferred. The pH conditioner is one or more selected from vitamin C, citric acid and L-lactic acid, and is prepared by adding appropriate amount of soap base and adjusting pH value of the soap base to 5-6.5.

The production method of the agilawood soap is characterized by comprising the following steps:

(1) kneading and weighing the soap base, the agilawood essential oil enrichment liquid, the tremella extract and the quality conditioner according to a ratio, adding the weighed materials into a conventional soap making kneader, kneading and stirring uniformly for 15-30 minutes at the stirring temperature of 30-40 ℃;

(2) grinding, namely adding the kneaded and uniformly stirred material into a grinder, wherein the thickness of the ground soap flakes is 0.2-0.4 mm;

(3) putting the ground soap flakes into a vacuum plodder by a vacuum layering machine, and performing vacuum layering, wherein the temperature of a plodder muzzle is 40 ℃, and the temperature of cooling water introduced into the plodder is 3-10 ℃;

(4) printing, namely putting the pressed strip soap body into a printer for printing, and controlling the temperature of a mould to be between-10 and-25 ℃ to obtain the artificial agilawood scented soap.

According to the invention, the artificial aquilaria wood with corresponding sterilization capability is obtained by artificially inoculating specific strains, and then the sterilization effective components are enriched by supercritical and molecular distillation, so that the agilawood essential oil enriched liquid with further enhanced sterilization capability is obtained. Meanwhile, the tremella is used as a quality modifier of the agilawood soap, so that the problems that the agilawood soap is easy to crack and the skin is dry and rough are solved.