阅读说明：本技术 一种小蓟总黄酮的提取方法 (Extraction method of herba cepbalanoplosis segeti total flavonoids ) 是由 毛垣元 张宁慧 田俊 李永新 彭梓洋 王颂恩 刘曼 杨坤龙 于 2020-07-29 设计创作，主要内容包括：本发明提供了一种小蓟总黄酮的提取方法,包括以下步骤：将小蓟烘干粉碎,水提醇沉法制得小蓟总黄酮浓缩液的步骤、冷冻干燥成粉的步骤；本发明以小蓟为原料,采用水提醇沉的方法,旋转蒸发,冷冻机冻干处理后得到小蓟提取物粉末,内含大量总黄酮,是非常强的自由基消除剂以及单线态氧消除剂,可抑制脂质的过氧化作用。其制作方法不仅相比于目前常用的植物总黄酮提取方法有较高的得率,而且其工艺相对简单。并且在此基础上,研究了小蓟总黄酮的抗氧化活性,为天然解酒护肝饮料的研究与开发利用提供了理论依据。(The invention provides a method for extracting total flavonoids of herba cepbalanoplosis segeti, which comprises the following steps: drying and crushing herba cepbalanoplosis segeti, extracting with water and precipitating with ethanol to obtain herba cepbalanoplosis segeti total flavone concentrated solution, and freeze-drying to obtain powder; the invention takes herba cepbalanoplosis segeti as raw material, adopts the method of water extraction and alcohol precipitation, rotary evaporation and freeze drying treatment by a refrigerator to obtain herba cepbalanoplosis segeti extract powder which contains a large amount of general flavone, is a very strong free radical scavenger and a singlet oxygen scavenger and can inhibit the peroxidation of lipid. Compared with the prior common plant total flavone extraction method, the preparation method has higher yield and relatively simple process. On the basis, the antioxidant activity of the total flavonoids of herba cephalanoploris is researched, and a theoretical basis is provided for research, development and utilization of natural anti-alcoholism and liver-protection beverages.)

1. A method for extracting total flavonoids from herba cepbalanoplosis segeti is characterized by comprising the following steps:

s1: oven drying herba Cephalanoploris to constant weight, pulverizing, and sieving to obtain herba Cephalanoploris powder;

s2: leaching the pulverized herba Cephalanoploris by ultrasonic-assisted extraction method to obtain herba Cephalanoploris total flavone crude extract solution;

s3: carrying out rotary evaporation on the herba cepbalanoplosis segeti solution until the volume is 1/3, thus obtaining a herba cepbalanoplosis segeti crude extraction concentrated solution;

s4: and (5) adding ethanol into the concentrated solution obtained in the step (S3) by crude extraction of the herba cepbalanoplosis segeti total flavonoids, precipitating, washing, drying, ventilating until the ethanol is volatilized, and freeze-drying to obtain the herba cepbalanoplosis segeti total flavonoids.

2. The method for extracting herba cepbalanoplosis segeti according to claim 1, wherein the step S1 specifically comprises: selecting fresh herba Cephalanoploris without mildew and damage, oven drying, pulverizing, and sieving with at least 40 mesh sieve to obtain herba Cephalanoploris powder.

3. The method for extracting herba cepbalanoplosis segeti according to claim 1, wherein the step S2 specifically comprises: taking 1 part of herba Cirsii powder, adding at least 20 parts of water, and leaching at 50 deg.C under ultrasonic power of 150W for at least 52 min to obtain herba Cirsii total flavone crude extract solution.

4. The method for extracting herba cepbalanoplosis segeti according to claim 1, wherein the step S3 specifically comprises: and (3) putting the solution into a rotary evaporator for vacuum concentration to obtain a concentrated solution of crude extraction of the herba cephalanoploris total flavonoids, wherein the temperature during rotary evaporation is 45 ℃.

5. The method for extracting herba cepbalanoplosis segeti according to claim 1, wherein the step S4 specifically comprises: precipitating the concentrated solution with 60% ethanol, standing for 24 hr, centrifuging at 4000r/min for 10min, washing and drying the precipitate, volatilizing ethanol in ventilated place, freezing at-20 deg.C, and lyophilizing in a lyophilizer for at least 24 hr to obtain herba Cephalanoploris total flavonoids.

Technical Field

The invention relates to food processing, in particular to a method for extracting total flavonoids of herba cepbalanoplosis segeti.

Background

Common cephalanoplos are distributed in most areas of China, and generally live in wastelands, grasslands, hillside forests, roadside places, shrubbery centers, fields, forest edges and creeks. In the full-bloom stage of 5-6 months, the whole grass is cut and dried in the sun or fresh, and the whole grass is rich in total flavonoids. The total flavone mainly contains flavonoids with high polarity, and has activating effect on alcohol dehydrogenase. Research results show that the herba Cephalanoploris ethanol extract can improve the activity of alcohol dehydrogenase and has the effect of relieving alcoholism. At present, the existing total flavone extraction method mostly adopts a hot water extraction method, the method is low in cost and simple to operate, but only a small amount of total flavone can be extracted, and the method is long in time consumption and needs to be improved urgently.

Disclosure of Invention

The invention aims to provide a novel extraction method of herba cepbalanoplosis segeti total flavonoids.

In order to achieve the above purpose, the technical scheme of the invention is as follows:

s1: oven drying herba Cephalanoploris to constant weight, pulverizing, and sieving to obtain herba Cephalanoploris powder;

s2: leaching the pulverized herba Cephalanoploris by ultrasonic-assisted extraction method to obtain herba Cephalanoploris total flavone crude extract solution;

s3: carrying out rotary evaporation on the herba cepbalanoplosis segeti solution until the volume is 1/3, thus obtaining a herba cepbalanoplosis segeti crude extraction concentrated solution;

s4: and (5) adding ethanol into the concentrated solution obtained in the step (S3) by crude extraction of the herba cepbalanoplosis segeti total flavonoids, precipitating, washing, drying, ventilating until the ethanol is volatilized, and freeze-drying to obtain the herba cepbalanoplosis segeti total flavonoids.

Compared with the prior art, the invention has the beneficial effects that:

the invention takes common cephalanoplos herb as raw material, adopts a method of ultrasonic-assisted water extraction and alcohol precipitation, and obtains common cephalanoplos herb total flavone with the functions of removing free radicals and inhibiting lipid oxidation after vacuum concentration, centrifugation and freeze drying; compared with the conventional common plant total flavone extraction method, the preparation method has the advantages of higher yield, simple operation, shorter time consumption and purer obtained total flavone. Provides a theoretical basis for the research, development and utilization of the natural beverage for dispelling the effects of alcohol and protecting the liver.

Drawings

FIG. 1 is a bar graph showing the effect of different feed liquid ratios on the extraction rate of total flavonoids from Cirsium setosum;

FIG. 2 is a bar graph of the effect of different extraction times on the extraction rate of total flavonoids from Cirsium setosum;

FIG. 3 is a bar graph of the effect of different extraction temperatures on the extraction rate of total flavonoids from Cirsium setosum;

FIG. 4 is a bar graph of the effect of different ultrasound powers on the extraction rate of total flavonoids from Cirsium setosum;

FIG. 5 is a bar graph of the effect of different ethanol concentrations on the extraction of total flavonoids from Cirsium setosum;

FIG. 6 is a response surface graph of the effect of the interaction of the feed-liquid ratio and time on the extraction rate of total flavonoids;

FIG. 7 is a graph of response of the interaction of ethanol concentration and time on the total flavone extraction rate;

FIG. 8 is a response surface graph showing the effect of the interaction of the feed-liquid ratio and the ethanol concentration on the total flavone extraction rate.

The specific implementation mode is as follows:

the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.