阅读说明：本技术 血浆维生素d水平与食管鳞癌发生的关系的研究方法 (Research method for relation between plasma vitamin D level and esophageal squamous cell carcinogenesis ) 是由 纪爱芳 于 2020-07-21 设计创作，主要内容包括：本发明适用于食管鳞癌研究的技术领域,提供了血浆维生素D水平与食管鳞癌发生的关系的研究方法,首先通过收集不同地区的患者、健康和健康的移民人群作为研究组,然后采用食物频率调查问卷法对所有研究对象进行膳食调查,评估维生素D摄入水平,接着进行统计学处理,最后比较各组维生素D水平,进行血浆维生素D水平与食管鳞癌发生的关系的研究判断,本发明操作简单,可以为血浆维生素D水平与食管鳞癌发生的关系提供有效的理论依据。(The invention is applicable to the technical field of esophageal squamous cell carcinoma research, and provides a method for researching the relationship between the plasma vitamin D level and the occurrence of esophageal squamous cell carcinoma.)

1. The method for researching the relation between the level of the blood plasma vitamin D and the occurrence of esophageal squamous cell carcinoma is characterized in that: the method comprises the following steps:

s1, collecting a preset number of esophageal squamous carcinoma patients as a research group A1; collecting a preset number of patients without esophageal, cardiac and gastric mucosal carcinogenesis from a as a research group A2; collecting a preset number of patients without esophageal, cardiac and gastric mucosal carcinogenesis of the B place as a research group B1; collecting a predetermined number of esophageal squamous carcinoma patients of B as study group B2; collecting a preset number of patients with esophageal cancer, cardia cancer and gastric mucosa cancer in the place a and b ethnic descendents as a research group C;

s2, performing diet survey on all the study subjects by adopting a food frequency questionnaire method, and evaluating the vitamin D intake level;

s3, detecting the level of the blood plasma vitamin D of 5 groups of study groups of people by adopting an ELISA method;

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s4, statistical treatment: processing data by using SPSSl9.0 statistical software; the measurement data is expressed by x +/-s, the mean between 5 groups is compared by variance analysis, and the comparison between every two groups is carried out by a Bonfereni method; wherein differences are considered statistically significant when P < 0.05;

and S5, comparing the vitamin D levels of all groups, and researching and judging the relation between the plasma vitamin D level and the occurrence of esophageal squamous cell carcinoma.

2. The method of claim 1 for studying the relationship between plasma vitamin D levels and esophageal squamous carcinogenesis, comprising: in step S2, the intake of vitamin D is derived from a dietary and nutritional supplement.

3. The method of claim 1 for studying the relationship between plasma vitamin D levels and esophageal squamous carcinogenesis, comprising: in step S3, the ELISA method is used to detect plasma vitamin D levels in 5 study groups of population, specifically:

sample treatment: mixing sodium citrate or ethylenediamine tetraacetic acid as anticoagulant for 10min, centrifuging at 2000r/min for 15min, collecting supernatant, and freezing at-20 deg.C;

the method comprises the following operation steps: according to the instruction of a human vitamin D enzyme-linked immunoassay kit;

calculating vitamin D levels: and calculating the concentration of the sample according to the concentration of the standard substance and the corresponding absorbance value.

Technical Field

The invention belongs to the technical field of esophageal squamous carcinoma research, and particularly relates to a method for researching the relationship between the level of plasma vitamin D and the occurrence of esophageal squamous carcinoma.

Background

Vitamin D deficiency is closely related to various chronic metabolic diseases including tumors, autoimmune diseases, diabetes, cardiovascular diseases and the like; several studies have also shown that vitamin D has a significant impact on the prognosis of patients with tumors. 2J, and 2J. Henan Linzhou (Yuan Lin county) is one of the world regions with high incidence of esophageal cancer, J, and partial residents in the northern region of J move to Shanxi Changzhi area before 113 years, so that a more concentrated natural immigration village is formed at present, more than 98% of the residents are descendants of Linzhou, the living environment and the eating habits of the Henan Linzhou are greatly changed from those in the Linzhou area, and are similar to the local situation of Shanxi Changzhi. In the research, ELISA method is used for detecting the vitamin D level in the blood plasma of 5 groups of patients suffering from the esophageal squamous carcinoma of Henan Linzhou, healthy people suffering from Shanxi Changzhi Long-Zhi, patients suffering from the esophageal squamous carcinoma of Shanxi and healthy people suffering from Changzhi immigration, aiming at discussing the relationship between the vitamin D level in the blood plasma and the occurrence of the esophageal squamous carcinoma.

Disclosure of Invention

The invention provides a method for researching the relation between the level of plasma vitamin D and the occurrence of esophageal squamous cell carcinoma, and aims to provide more theoretical bases for researching the occurrence factors of esophageal squamous cell carcinoma.

The invention is realized by the method for researching the relation between the level of the blood plasma vitamin D and the occurrence of esophageal squamous cell carcinoma, which comprises the following steps:

s1, collecting a preset number of esophageal squamous carcinoma patients as a research group A1; collecting a preset number of patients without esophageal, cardiac and gastric mucosal carcinogenesis from a as a research group A2; collecting a preset number of patients without esophageal, cardiac and gastric mucosal carcinogenesis of the B place as a research group B1; collecting a predetermined number of esophageal squamous carcinoma patients of B as study group B2; collecting a preset number of patients with esophageal cancer, cardia cancer and gastric mucosa cancer in the place b as a research group C;

s2, performing diet survey on all the study subjects by adopting a food frequency questionnaire method, and evaluating the vitamin D intake level;

s3, detecting the level of the blood plasma vitamin D of 5 groups of study groups of people by adopting an ELISA method;

s4, statistical treatment: processing data by using SPSSl9.0 statistical software; measure data to

Representing that the mean number between 5 groups is compared by variance analysis, and the comparison between every two groups is carried out by a Bonfereni method; wherein, P<0.05 the difference was considered statistically significant;

and S5, comparing the vitamin D levels of all groups, and researching and judging the relation between the plasma vitamin D level and the occurrence of esophageal squamous cell carcinoma.

Preferably, in step S2, the intake of vitamin D is derived from a dietary and nutritional supplement.

Preferably, in step S3, the ELISA method is used to detect the plasma vitamin D level of 5 study groups of human population, specifically:

sample treatment: mixing sodium citrate or ethylenediamine tetraacetic acid as anticoagulant for 10min, centrifuging at 2000r/min for 15min, collecting supernatant, and freezing at-20 deg.C;

the method comprises the following operation steps: according to the instruction of a human vitamin D enzyme-linked immunoassay kit;

calculating vitamin D levels: and calculating the concentration of the sample according to the concentration of the standard substance and the corresponding absorbance value.

Compared with the prior art, the invention has the beneficial effects that: according to the method for researching the relation between the plasma vitamin D level and the esophageal squamous cell carcinoma, firstly, patients in different areas and healthy immigration people are collected to serve as research groups, then dietary investigation is conducted on all research objects by adopting a food frequency questionnaire method, the vitamin D intake level is evaluated, then statistical treatment is conducted, finally, the vitamin D levels of all groups are compared, and research and judgment on the relation between the plasma vitamin D level and the esophageal squamous cell carcinoma are conducted.

Drawings

FIG. 1 is a schematic flow chart of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

Referring to fig. 1, the present invention provides a technical solution: the method for researching the relation between the level of the blood plasma vitamin D and the occurrence of esophageal squamous cell carcinoma comprises the following steps:

s1, dividing the research objects into 5 groups:

group A1: patients with esophageal squamous carcinoma in Henan Linzhou: 104 patients with esophageal squamous carcinoma who are subjected to gastroscopy and pathologically confirmed in the affiliated and peaceful hospital central hospital of the long medical school from 6 months to 5 months in 2012, 34 patients and 70 patients in women, 5 l-70 years old are collected;

group A2: henan Linzhou healthy population: the collection of patients with esophageal cancer, cardia cancer and gastric mucosa cancer was performed by gastroscopy in the same period in the subsidiary department of the medical institute of Long treatment and the central hospital of the peace Hospital, and 104 patients with esophageal cancer, cardia cancer and gastric mucosa cancer were confirmed, 56 patients were confirmed, 48 patients were confirmed, and the age was 48-72 years old.

Group B1: shanxi Changzhi health people: gastroscopy is carried out in the affiliated and peaceful hospital central hospital of the long treatment medical college in 2013, 9 months to 2014, and 104 patients without esophagus, cardia and gastric mucosa canceration, 60 men, 44 women and the age of 43-78 years are verified.

Group B2: shanxi Changzhi treatment of patients with esophageal squamous carcinoma: collecting 108 patients with esophageal squamous carcinoma, 48 patients with male and 60 patients with female, and the age of 45-75 years, which are pathologically confirmed, in the same period of gastroscopy in the subsidiary department of the Long treatment medical school and the central hospital of the peace Hospital.

Group C: healthy forest immigration population in the Changzhi area: gastroscopy is carried out in affiliated and peaceful hospital central hospitals of the institute of medical science of Long treatment in 2011, 6 months to 2013, 9 months, and 80 cases, 26 cases, 54 cases and the age of 44-77 years of morbid Linzhou migrant descendants without esophagus, cardia and gastric mucosa canceration are confirmed.

As shown in table 1 below:

s2 diet survey was performed on all subjects using the food frequency questionnaire to assess vitamin D intake levels. The intake of vitamin D is derived from dietary and nutritional supplements. Questionnaires display: the above 5 groups of people have substantially consistent vitamin D, and have no supplement of vitamin D-containing medicine.

S3, and detecting the level of the blood plasma vitamin D of the population of the 5 groups of research groups by adopting an ELISA method.

Sample treatment: mixing sodium citrate or ethylenediamine tetraacetic acid as anticoagulant for 10min, centrifuging at 2000r/min for 15min, collecting supernatant, and freezing at-20 deg.C;

the method comprises the following operation steps: according to the instruction of a human vitamin D enzyme-linked immunoassay kit;

calculating vitamin D levels: and calculating the concentration of the sample according to the concentration of the standard substance and the corresponding absorbance value.

S4, statistical treatment: processing data by using SPSSl9.0 statistical software; measure data toRepresenting that the mean number between 5 groups is compared by variance analysis, and the comparison between every two groups is carried out by a Bonfereni method; wherein, P<0.05 the difference was considered statistically significant.

As shown in table 2 below: comparison of vitamin D levels in each group (μ g/L,)

in the parentheses, the numbers are exemplified.

And S5, comparing the vitamin D levels of all groups, and researching and judging the relation between the plasma vitamin D level and the occurrence of esophageal squamous cell carcinoma.

In this example, the results are as follows:

the differences in total plasma vitamin D levels and plasma vitamin D levels of different gender and age were statistically significant (P was < 0.05). Two-by-two comparisons of plasma vitamin D levels were made with the bonffeeroni method for group 5:

and (3) overall level comparison: the difference between the linzhou healthy group and the linzhou esophageal cancer group has statistical significance (P < 0.05); the difference was statistically significant (P <0.05) in the linzhou esophageal cancer group compared to the linzhou immigration health group. Compared with the Linzhou healthy group, the difference of the long-treatment esophageal cancer group has statistical significance (P < 0.05); the difference between the long-treatment esophageal cancer group and the Linzhou immigration group has statistical significance (P < 0.05).

The female population is as follows: the difference between the linzhou healthy group and the linzhou esophageal cancer group has statistical significance (P < 0.05); the difference between the Linzhou esophageal cancer group and the Linzhou immigration group is statistically significant (P < 0.05).

People older than 60 years: the difference between the linzhou healthy group and the linzhou esophageal cancer group has statistical significance (P < 0.05); the difference between the esophagus cancer group and immigration group in Linzhou is statistically significant (P < 0.05); compared with the Linzhou healthy group, the difference of the long-treatment esophageal cancer group has statistical significance (P < 0.05); the difference between the long-treatment esophageal cancer group and the Linzhou immigration group has statistical significance (P < 0.05).

The occurrence of esophageal cancer is closely related to a variety of environmental, nutritional and genetic factors and their interactions. To date, factors that have been found to be significantly associated with the risk of esophageal carcinogenesis include age, family history, dietary factors, etc. H1. By effectively interfering with relevant factors such as diet and living habits, the purpose of reducing the risk of esophageal cancer can be achieved. In the occurrence and development of esophageal cancer, nutrient element and trace element deficiency, nitrite, viruses, some toxins and the like are considered to be important pathogenic factors. Previous research finds that the nutrient deficiency is a common phenomenon in high-incidence areas of esophageal cancer, and the vitamin D deficiency phenomenon is more obvious in the high-incidence areas of esophageal cancer. Recent evidence from population studies has shown that vitamin D deficiency is closely associated with a variety of chronic metabolic diseases including tumors, autoimmune diseases, diabetes, cardiovascular diseases, and the like. As early as 1937, Peller and Stephenson found that the incidence of tumors was lower in people exposed to sunlight for longer periods of time. In 1941, Aperly demonstrated a negative correlation between latitude height and tumor mortality in human habitats. Garland and colleagues found that people living at high latitudes have an increased incidence of breast, colorectal, ovarian cancer due to vitamin D deficiency in their bodies; schwartz and Hulka found that vitamin D deficiency was associated with the development of pancreatic cancer.

Following studies with lung and rectal cancer patients, it was found that the overall survival rate and life span of patients with low vitamin D levels in vivo is significantly lower than that of patients with normal and higher levels of vitamin D. Therefore, whether vitamin D deficiency is related to the occurrence of esophageal cancer and the specific mechanism is a question worthy of discussion.

The population of Linzhou immigration in Shanxi Changzhi area has the genetic background of the Linzhou population in the area with the highest incidence rate of esophageal cancer in the world, and has the living environment and the eating habit similar to those of local residents in the Changzhi area. The results of this study show that: differences exist in plasma vitamin D levels between the linzhou healthy group and the linzhou esophageal cancer group, the linzhou esophageal cancer group and the linzhou immigration group, the long-term esophageal cancer group and the linzhou healthy group, and the long-term esophageal cancer group and the linzhou immigration healthy group; the level of vitamin D in the plasma of the linzhou esophageal cancer group is significantly lower than that of the linzhou healthy group and the linzhou immigration healthy group, and the level of vitamin D in the plasma of long-term esophageal cancer patients is significantly lower than that of the linzhou immigration healthy group, which indicates that the phenomenon of vitamin D deficiency exists in esophageal squamous carcinoma patients. Meanwhile, the results of the stratified analysis of male, female and age groups show that the level of vitamin D in the plasma of the Linzhou healthy group and the Linzhou esophageal cancer group is also obviously different. Since the living environment and dietary habits of the 3 groups of the long treatment healthy group, the long treatment esophageal cancer group and the state immigration healthy group were similar, we considered that differences in the level of vitamin D in plasma due to different dietary vitamin D intakes could be excluded. We found earlier that there was a riboflavin deficiency in patients with esophageal squamous carcinoma. If the variation of the riboflavin transporter gene C20orf54 causes that riboflavin cannot effectively enter cells to play a normal role, the research result of the single nucleotide polymorphism (cSNP) site in the coding region of the C20orf54 gene shows that the sites rs3746802, rs3746803 and rs3746804 are obviously related to the genetic susceptibility of esophageal squamous cell carcinoma. This reveals to some extent the effect of environmental and genetic factor interactions on esophageal carcinogenesis. Vitamin D is biologically active 1, 25(OH) in vivo₂D₃The forms bind to vitamin D receptors and perform biological functions. Comprehensive prophase and current researchThe result shows that although the living environment and the eating habits of people moving people in Linzhou are similar to those of people for long-term treatment of esophageal cancer, the genetic background is different, and if the gene of a vitamin D receptor is mutated, the absorption of the body to vitamin D is influenced, so that the level of vitamin D in the body is reduced. Whether differences in the cSNP of vitamin D receptors are responsible for differences in vitamin D levels in vivo remains to be investigated further

According to the method for researching the relation between the plasma vitamin D level and the esophageal squamous cell carcinoma, firstly, patients in different areas and healthy immigration people are collected to serve as research groups, then dietary investigation is conducted on all research objects by adopting a food frequency questionnaire method, the vitamin D intake level is evaluated, then statistical treatment is conducted, finally, the vitamin D levels of all groups are compared, and research and judgment on the relation between the plasma vitamin D level and the esophageal squamous cell carcinoma are conducted.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.