阅读说明：本技术 效应因子RxLR129113表达的效应蛋白在促进植物生长方面的应用 (Application of effector protein expressed by effector factor RxLR129113 in promoting plant growth ) 是由 张修国 朱春原 李京 高克祥 李壮 于 2020-05-20 设计创作，主要内容包括：本发明涉及植物培育技术领域,具体公开了效应因子RxLR129113表达的效应蛋白在促进植物生长方面的应用。本发明通过对效应因子RxLR129113表达的效应蛋白的深入研究,将植物种子浸泡到含有效应蛋白的溶液中,欣喜地发现了其在促进植物种子萌发和幼芽生长方面具有显著的积极作用,并发现将含有效应蛋白的溶液加入至植物育苗基质中,能激发植物根系的生长与发育,进而促进幼苗的发育壮大。(The invention relates to the technical field of plant cultivation, and particularly discloses application of an effector protein expressed by an effector factor RxLR129113 in promoting plant growth. According to the invention, through deep research on effector protein expressed by an effector factor RxLR129113, plant seeds are soaked in the solution containing the effector protein, so that the obvious positive effect on promoting the germination of the plant seeds and the growth of sprouts is discovered at a happy place, and the growth and development of plant root systems can be stimulated by adding the solution containing the effector protein into a plant seedling raising substrate, thereby promoting the growth and development of seedlings.)

1. Application of effector protein expressed by effector factor RxLR129113 in promoting plant growth.

2. The application according to claim 1, wherein the application is any one of the following:

(1) promoting the germination of plant seeds;

(2) promoting the growth of plant buds;

(3) promoting the growth of plant roots;

(4) promoting the growth of plant seedlings.

3. The use according to claim 2, wherein the plant seeds are soaked with the solution containing the effector protein to promote seed germination and shoot growth;

or applying the solution containing the effector protein to a plant seedling raising substrate to promote the growth of plant roots and the development of seedlings.

4. Use according to any one of claims 1 to 3, wherein the plant is a dicotyledonous plant.

5. Use according to claim 4, wherein the plant is a plant of the order Violales.

6. Use according to claim 5, wherein the plant is a plant of the Cucurbitaceae family.

7. Use according to claim 6, wherein the plant is of the genus Cucumis.

8. The use of any one of claims 1 to 3, wherein the effector protein is induced to express by a genetically engineered bacterium containing an effector factor RxLR 129113.

9. A method of promoting germination or shoot growth of plant seeds, comprising soaking the plant seeds with a solution containing an effector protein expressed by effector factor RxLR 129113.

10. A method for promoting the growth of plant roots or the development of seedlings is characterized in that effector protein expressed by an effector factor RxLR129113 is added into a seedling substrate.

Technical Field

The invention relates to the technical field of plant cultivation, in particular to application of effector protein expressed by an effector factor RxLR129113 in promoting plant growth.

Background

The long-term dependence on chemical fertilizers has also raised some problems in promoting plant growth and increasing yield. Such as: (1) the nitrogen and phosphorus contents in water are increased, so that the eutrophication of rivers, lakes and internal seas causes excessive propagation of algae, deterioration of water quality and mass death of fishes and other aquatic organisms; (2) the soil can be acidified by simply applying chemical fertilizers in an excessive way for a long time. The ammonium ion content of organic and inorganic complexes in the soil solution and on the soil micelle is increased, the soil structure is damaged, the soil is hardened, and the agricultural production cost and the crop yield are directly influenced; (3) toxic components in food, feed and drinking water are increased, and the nitrogen compound content in well water and river water in fertilizer areas is increased and even exceeds the drinking water standard; (4) the soil microflora is destroyed. The excessive application of chemical fertilizers, especially nitrogen fertilizers, has killing and inhibiting effects on microorganisms, a large number of microorganisms die after long-term application, soil microbial flora changes, a plurality of beneficial microorganisms change from dominant species to secondary species, and crops are easy to suffer from various diseases.

The root system is an important absorption, synthesis, fixation and support organ of the plant, plays an important role in the growth and development process of the crop, and the robust root system can provide sufficient nutrients and water for the growth of the plant, thereby being the basis of high yield of the plant. At present, root research is internationally taken as a basic research topic with great potential for further improving crop productivity.

Disclosure of Invention

In order to solve the problems in the prior art, the invention aims to provide a new application of an effector protein expressed by an effector factor RxLR129113 in promoting the growth and development of plants.

Another purpose of the invention is to provide a method for promoting the germination of plant seeds or the growth of buds and the growth of plant roots or the development of seedlings.

It should be noted that the effector factor RxLR129113 described in the present invention has been described in chinese patent application publication No. CN110878315A (publication No. 03/13/2020), and the nucleotide sequence and the amino acid sequence of the expressed effector protein are not described in detail in the present invention.

The technical scheme provided by the invention is as follows:

in a first aspect, the present invention provides the use of an effector protein expressed by the effector factor RxLR129113 for promoting plant growth.

The application is the application of any one of the following aspects:

(1) promoting the germination of plant seeds;

(2) promoting the growth of plant buds;

(3) promoting the growth of plant roots;

(4) promoting the growth of plant seedlings.

More specifically, plant seeds are soaked by using a solution containing the effector protein, so that the germination of the seeds and the growth of sprouts are promoted; or by applying the solution containing the effector protein to a plant seedling raising substrate, the growth of plant root systems and the development of seedlings are promoted.

Alternatively, the plant of the present invention is a dicotyledonous plant, preferably a plant of the order violales, more preferably a plant of the family Cucurbitaceae (Cucurbitaceae), and even more preferably a plant of the genus Cucumis (Cucumis).

Experiments show that the effector protein expressed by the effector factor RxLR129113 has obvious positive effects on promoting seed germination, sprout growth, root growth, seedling development and the like of cucumber (Cucumis sativus). Furthermore, it is also assumed that plants of the genus Cucumis, which have a close relationship with Cucumis sativus, such as Cucumis bisexualis, Cucumis hystrix Chakrusei, Cucumis melo (Cucumis melo L.), and varieties thereof, such as Cucumis melo (Cucumis melo L.var. chinensis Pangal) can be promoted in terms of growth and development by the above effector proteins.

Furthermore, the effector protein can construct a genetic engineering bacterium containing an effector factor RxLR129113 by a genetic recombination means, and the effector factor is induced and expressed to obtain the effector protein.

In a second aspect, the present invention provides a method of promoting germination or shoot growth of a plant seed by soaking the plant seed with a solution containing an effector protein expressed by the effector factor RxLR 129113.

And the method for promoting the growth of plant root systems or the development of seedlings is characterized in that effector protein expressed by an effector RxLR129113 is added into a seedling raising matrix, and the plant seedlings are placed in the matrix for cultivation, so that the growth of the root systems of the plant seedlings is promoted, and the development of the seedling is more robust.

The raw materials or reagents involved in the invention are all common commercial products, and the operations involved are all routine operations in the field unless otherwise specified.

The above-described preferred conditions may be combined with each other to obtain a specific embodiment, in accordance with common knowledge in the art.

The invention has the beneficial effects that:

according to the invention, through deep research on effector protein expressed by effector factor RxLR129113, plant seeds are soaked in the solution containing the effector protein, positive effects of the effector protein on promoting germination of the plant seeds and growth of sprouts are discovered with pleasure, and the solution containing the effector protein is added into a seedling raising substrate, so that growth of plant root systems can be stimulated, and further growth and development of seedlings are promoted.

The invention is based on the prior invention and carries out the expanded research on the function of an effector RxLR 129113. Not only broadens the application range of the effect factor, but also provides a new inspiration and solution for plant cultivation technology.

Drawings

FIG. 1 is a map of pET28a vector in example 1 of the present invention;

FIG. 2 shows the result of PCR amplification of the RxLR129113 gene in example 1 of the present invention; wherein, M: DNA Marker, 1-4: PCR amplification band of RxLR129113 gene;

FIG. 3 is an SDS-PAGE gel analysis of the test expression of RxLR129113 in example 1 of the present invention; wherein, M: proteinmarker; 1-2: IPTG induces RxLR129113 protein expression;

FIG. 4 shows the effect of RxLR129113 protein solution on the shoot growth of cucumber seeds in example 1.

FIG. 5 shows the effect of the growth medium added with RxLR129113 protein solution on the growth vigor of 2-leaf stage cucumber seedlings in example 1 of the present invention.

FIG. 6 shows the effect of the seedling substrate added with RxLR129113 protein solution on the growth of cucumber seedlings in 4-5 leaf stage in example 1 of the present invention.

Detailed Description

Preferred embodiments of the present invention will be described in detail with reference to the following examples. It is to be understood that the following examples are given for illustrative purposes only and are not intended to limit the scope of the present invention. Various modifications and alterations of this invention will become apparent to those skilled in the art without departing from the spirit and scope of this invention.

The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.

Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.