阅读说明：本技术 一种腺苷一磷酸在促进动物采食和/或改善肉质中的应用 (Application of adenosine monophosphate in promoting animal feeding and/or improving meat quality ) 是由 谭成全 李佳颖 印遇龙 邓*** 邓铭 杨芸瑜 于 2020-08-11 设计创作，主要内容包括：本发明属于食品及饲料技术领域,具体涉及一种腺苷一磷酸在促进动物采食和/或改善肉质中的应用。本发明研究发现腺苷一磷酸可通过诱食缩短小鼠采食间隔时间,增加小鼠对可口食物的偏好性提高采食量,另一方面,腺苷一磷酸可显著增加小鼠下丘脑的促采食神经元AgRP蛋白水平,而对抑采食神经元POMC蛋白水平无明显影响,进而调控机体采食行为；同时通过试验发现腺苷一磷酸能够改善小鼠体成分和显著提高小鼠能量代谢,增加肌肉含量,降低脂肪含量,降低血清中的支链氨基酸含量,增加肌肉中风味氨基酸的含量,说明腺苷一磷酸能够增加机体肌肉含量改善肉品质。本发明为促进采食及改善肉质方面提供了一种新的方式。(The invention belongs to the technical field of food and feed, and particularly relates to application of adenosine monophosphate to promotion of animal feeding and/or improvement of meat quality. The research of the invention finds that adenosine monophosphate can shorten the interval time of mouse feeding by food calling and increase the preference of the mouse to delicious food to improve the feeding amount, on the other hand, adenosine monophosphate can obviously increase the level of the feeding promoting neuron AgRP protein of the hypothalamus of the mouse, but has no obvious influence on the level of the feeding inhibiting neuron POMC protein, thereby regulating and controlling the feeding behavior of the organism; meanwhile, experiments show that adenosine monophosphate can improve the body components of mice, remarkably improve the energy metabolism of the mice, increase the muscle content, reduce the fat content, reduce the branched chain amino acid content in serum and increase the content of flavor amino acid in muscles, and therefore the adenosine monophosphate can increase the muscle content of organisms and improve the meat quality. The invention provides a new way for promoting food intake and improving meat quality.)

1. Use of adenosine monophosphate for promoting animal feeding and/or improving meat quality.

2. Use of adenosine monophosphate according to claim 1 for promoting feed intake and/or meat quality improvement in animals, characterized in that:

the adenosine monophosphate promotes the animals to eat the feed so as to increase the cumulative feed intake of the animals.

3. An application of adenosine monophosphate in shortening the interval time of animal feeding and improving the feed intake of mice at night and throughout the day.

4. Use of adenosine monophosphate for increasing energy metabolism in an animal.

5. An application of adenosine monophosphate in promoting expression of hypothalamic appetizing neuron AgRP protein.

6. Use of adenosine monophosphate for improving body composition, increasing muscle content and reducing fat content in an animal.

7. An application of adenosine monophosphate in reducing the content of branched chain amino acids in animal serum is provided.

8. An application of adenosine monophosphate in increasing the content of flavor amino acids in animal muscle and improving meat quality is provided.

9. A product for increasing feed intake and/or improving meat quality, characterized by comprising adenosine monophosphate.

10. A feed for increasing feed intake and/or improving meat quality, characterized by comprising adenosine monophosphate, wherein adenosine monophosphate is added in an amount of 1g per kg of feed.

Technical Field

The invention belongs to the technical field of food and feed, and particularly relates to application of adenosine monophosphate to promotion of animal feeding and/or improvement of meat quality.

Background

Feeding is the basis for animals to obtain nutrition for maintaining life activities and promoting growth and development. In animal husbandry, factors such as feed, environment, management and diseases all affect the feed intake of animals. Neurons secreted by hypothalamic appetite regulating neurons, such as AgRP, POMC and the like, can regulate and control the feeding behavior of animals.

Guangdong and Guangxi are in tropical rain forest regions, and high temperature and high humidity are the most typical weather and climate characteristics in summer, so that the feed intake of live pigs in the later fattening period is seriously reduced, and the growth and development of the live pigs are influenced. Poor appetite of live pigs for a long time before slaughtering can not only reduce the weight of the pigs on the market, but also can cause PSE meat due to serious high-temperature stress, reduce the pork quality, reduce the meat tenderness, have poor taste, and can not be accepted by consumers or even cause serious economic loss.

The main flavor amino acids such as aspartic acid and glutamic acid in pork have direct relation with the flavor of the pork, which is one of important prerequisite substances for forming delicate flavor substances, determines the pork flavor and is a prerequisite for guaranteeing the pork quality.

Adenosine Monophosphate (AMP) has the effects of improving insulin resistance and impaired glucose metabolism, however, studies on the aspects of adjusting the ingestion of mammals and improving meat quality by adenosine monophosphate have not been reported in detail.

Disclosure of Invention

In order to overcome the defects and shortcomings of the prior art, the invention aims to provide the application of adenosine monophosphate in promoting animal feeding and/or improving meat quality so as to achieve the purposes of increasing the feeding intake of an organism and improving the meat quality.

The above purpose of the invention is realized by the following technical scheme:

use of adenosine monophosphate for promoting animal feeding and/or improving meat quality;

the adenosine monophosphate promotes the animals to eat, namely increases the cumulative feed intake of the animals;

the adenosine monophosphate is applied to shortening the interval time of animal feeding and improving the feed intake of mice at night and all day;

the use of said adenosine monophosphate for increasing energy metabolism in an animal;

the application of adenosine monophosphate in promoting expression of hypothalamic appetizing neuron AgRP protein;

the use of adenosine monophosphate for improving body composition, increasing muscle content and reducing fat content of an animal;

the application of the adenosine monophosphate in reducing the content of branched chain amino acids in animal serum;

the adenosine monophosphate is applied to increasing the content of flavor amino acid in animal muscle and improving meat quality;

a product for increasing feed intake and/or improving meat quality comprising adenosine monophosphate;

preferably, the product includes food, medicine, health products, etc.;

a feed for increasing feed intake and/or improving meat quality comprises adenosine monophosphate, wherein the addition amount of adenosine monophosphate in each kilogram of feed is 1 g;

through animal feeding experiments, the inventor finds that the appetite of a mouse can be obviously improved by adding adenosine monophosphate into a mouse feed, the feeding interval time of the mouse is shortened, the protein expression level of the feeding promoting neuron AgRP of the hypothalamus of the mouse is improved, and the accumulated feeding amount of the mouse is increased.

The inventor takes C57BL/6J mice at 4 weeks as the research object, and takes adenosine monophosphate mice with the dosage of 0.1% for the mice to eat freely, so as to explore the influence of adenosine monophosphate on the eating index and the amino acid content of the mice. The test shows that: adenosine monophosphate can shorten the interval time of ingestion of mice, obviously improve the protein expression level of the mice hypothalamus feeding promoting neuron AgRP, and has no obvious influence on the protein expression quantity of the feeding inhibiting neuron POMC. Meanwhile, adenosine monophosphate can improve body components of mice, increase energy metabolism, reduce the content of serum branched chain amino acid, increase the content of flavor amino acid in muscles, and further increase the content of muscle and reduce the content of fat in the mice.

Compared with the prior art, the invention has the following beneficial effects:

experiments show that the adenosine monophosphate can obviously improve the appetite of organisms and improve the meat quality of the organisms, provides a new application of the adenosine monophosphate, provides a new mode for increasing the feed intake of the organisms and improving the meat quality, is one of precursors of important energy substances in organisms, and has good popularization and application prospects.

Drawings

FIG. 1 is a graph of the effect of adenosine monophosphate on feed intake and body weight in mice; where AMP represents "adenosine monophosphate", a: cumulative feed intake (Cumulative feed intake) detection result, B: weight (Body weight) test results.

FIG. 2 is a graph of the effect of adenosine monophosphate on the meal pattern of mice; wherein Day represents "Day", Night represents "Night", Total represents "whole Day", a: meal intake (Meal size) test result, B: number of ingested meals (Number of foods) test result, C: food intake (Food intake) test results, D: feeding duration (Mean duration) test result, E: and (4) detecting the result of the feeding interval (Mean average bout length).

FIG. 3 is a graph of the effect of adenosine monophosphate on mouse energy metabolism; wherein, A: minimum energy expenditure (MinimumEE), B: maximum energy expenditure (Maximum EE), C: average Energy Expenditure (EE), D: carbon dioxide release amount (VCO2), E: oxygen consumption (VO2), F: respiratory entropy (RQ), where the upper right hand graph of CDEF is EE, VCO2, VO2, and RQ circadian line graphs, respectively.

FIG. 4 is a graph showing the effect of adenosine monophosphate on the expression level of appetite-related protein in mouse hypothalamus; wherein AgRP stands for "acanthosis-associated protein", POMC for "pro-opiomelanocortin", β -actin for "actin", a: immunoblotting of AgRP, B: expression statistics of AgRP, C: immunoblotting of POMC, D: statistics of POMC expression.

FIG. 5 is a graph of the metabolic kinetics of adenosine monophosphate and adenosine; wherein, Adenosine stands for "Adenosine", a: content of AMP in serum at different times after gavage, B: the content of adenosine in serum at different times after gastric lavage.

FIG. 6 is a graph of the effect of adenosine monophosphate on body composition in mice; wherein Body mass represents "Body composition content", lean mass represents "lean meat content", and fat mass represents "fat content".

FIG. 7 is a graph of the effect of adenosine monophosphate on the amino acid content in mouse serum and muscle; wherein Amino acids represent "Amino acids", Serum represents "Serum", and tibials antigen muscle represents "Tibialis anterior", a: content of amino acids in serum, B: the content of amino acids in the tibialis anterior.

Detailed Description

The present invention will be described in further detail with reference to examples and drawings, but the present invention is not limited thereto.

Unless otherwise indicated, reagents and materials used in the following examples are commercially available.