阅读说明：本技术 含有解肽假交替单胞菌的培养物或其提取物的用于皮肤美白的组合物 (Composition for skin whitening comprising culture of pseudoalteromonas peptaiytica or extract thereof ) 是由 明吉仙 高在濚 金容震 于 2018-09-14 设计创作，主要内容包括：本发明公开了一种用于皮肤美白的组合物,其包含作为有效成分的以下物质：解肽假交替单胞菌菌株,其裂解物,其培养物,或所述菌株、其裂解物或其培养物的提取物。此外,本发明还公开了一种具有美白功能的、保藏编号为KCCM12050P的解肽假交替单胞菌SNC 130菌株。(The invention discloses a composition for skin whitening, which comprises the following substances as effective ingredients: a peptide-degrading pseudoalteromonas strain, a lysate thereof, a culture thereof, or an extract of said strain, a lysate thereof, or a culture thereof. In addition, the invention also discloses a peptide-decomposing pseudoalteromonas SNC130 strain with a whitening function and a preservation number of KCCM 12050P.)

1. A composition for skin whitening comprising as active ingredients: a peptide-degrading pseudoalteromonas strain, a lysate thereof, a culture thereof, or an extract of said strain, a lysate thereof, or a culture thereof.

2. The composition for skin whitening according to claim 1, characterized in that the strain is pseudoalteromonas depsipeptide SNC130 with the accession number KCCM 12050P.

3. The composition for skin lightening according to claim 2, wherein the strain has a sequence comprising seq id No.: 1, and a 16S rDNA having the base sequence shown in 1.

4. The composition for skin whitening according to claim 1, characterized in that the culture is obtained by culturing in a medium comprising one or more substances selected from starch, yeast extract, peptone and sea salt.

5. The composition for skin whitening according to claim 1, characterized in that the extract is an ethyl acetate fraction.

6. The composition for skin whitening according to claim 1, characterized in that the content of the pseudoalteromonas depsipeptide strain, its lysate, its culture, or the extract of the strain, its lysate or its culture, is comprised between 0.001% and 30% by weight of the total weight of the composition.

7. The composition for skin lightening according to claim 1, wherein the composition inhibits melanin production or inhibits tyrosinase activity.

8. Composition for skin lightening according to claim 1, characterized in that it is a cosmetic composition.

9. A peptide-dissolving pseudoalteromonas SNC130 strain, wherein the deposit number of the peptide-dissolving pseudoalteromonas SNC130 is KCCM 12050P.

10. The strain of claim 9, wherein the strain has a skin whitening effect.

Technical Field

The invention discloses a composition for skin whitening, which comprises the following substances as effective ingredients: a peptide-degrading pseudoalteromonas strain, a lysate thereof, a culture thereof, or an extract of said strain, a lysate thereof, or a culture thereof.

Background

Human skin color depends on carotene, melanin content and hemoglobin, with melanin playing the most important role. Melanin is not only a pigment related to skin color, but also a pigment related to hair and pupil color, and plays a protective role in human skin. However, if melanin is excessively produced in the skin due to excessive exposure to external environments such as ultraviolet rays, air pollution, pressure, etc., a phenomenon of pigmentation is caused, thereby causing skin blackening, or spots, freckles, etc. to be generated. Ultraviolet rays are a main factor causing excessive production of melanin by promoting the activity of melanocytes, which are cells that produce melanin, promoting the secretion of melanin biosynthesis stimulating hormones, promoting the oxidation of melanin, promoting the activity of tyrosinase, and the like. The most characteristic of this melanin production mechanism is that it involves an enzyme called tyrosinase, and if the tyrosinase activity is inhibited to prevent melanin production, it is expected to produce a whitening effect.

On the other hand, a strain of Pseudoalteromonas (Pseudoalteromonas) is one of marine microorganisms, has a property of being able to form a biofilm, but the effect of skin whitening thereof has not been disclosed, and among them, a depsipeptide Pseudoalteromonas (Pseudoalteromonas pepticolytica) strain was discovered as a new species by japanese researchers since 2000, and a use scheme of the strain has not been disclosed.

[ Prior art documents ]

[ patent document ]

Korean patent laid-open No. 10-0812922.

Disclosure of Invention

Technical problem

In one aspect, the present invention aims to provide a novel use of a peptide-degrading pseudoalteromonas (pseudoalteromonas peptidolytica) strain.

In another aspect, the present invention aims to provide a pseudoalteromonas depsipeptide SNC130 strain having excellent skin whitening activity.

Technical scheme

In one aspect, the disclosed technology provides a composition for skin whitening comprising as active ingredients: a strain of Pseudomonas peptostrytica (Pseudomonas peptidolytica), a lysate thereof, a culture thereof, or an extract of said strain, a lysate thereof, or a culture thereof.

In an exemplary embodiment, the strain may be pseudoalteromonas depsipeptide SNC130 with accession number KCCM 12050P.

In one exemplary embodiment, the strain may have a sequence comprising SEQ ID No.: 1, and a 16S rDNA having the base sequence shown in 1.

In an exemplary embodiment, the culture may be obtained by culturing in a medium comprising one or more substances selected from the group consisting of starch, yeast extract, peptone, and sea salt.

In an exemplary embodiment, the extract may be an ethyl acetate fraction.

In an exemplary embodiment, the active ingredient may be present in an amount of 0.001 to 30 wt% based on the total weight of the composition.

In an exemplary embodiment, the composition can inhibit melanin production or inhibit tyrosinase activity.

In one exemplary embodiment, the composition may be a cosmetic composition.

In another aspect, the disclosed technology provides a Pseudoalteromonas peptidolyticus (Pseudoalteromonas peptidolyticum) SNC130 strain having skin whitening function with the accession number of KCCM 12050P.

In an exemplary embodiment, the strain may be a strain isolated from an alga.

Advantageous effects

In one aspect, the disclosed technology has the effect of providing a skin whitening raw material that is an environmentally friendly material.

In another aspect, the disclosed technology has the effect of providing the use of a Pseudoalteromonas peptioides (pseudomonas peptidolytica) strain for skin whitening. The strain has the effects of inhibiting melanin generation in melanin-producing cells and inhibiting the activity of enzyme for producing melanin.

In another aspect, the disclosed technology has the effect of providing a Pseudoalteromonas peptidolyticus (SNC 130) strain having excellent skin whitening activity.

Drawings

FIG. 1 shows the 16s rDNA sequence (SEQ ID NO: 1) of the peptaibolytic Pseudoalteromonas (Pseudomonas peptidolytica) SNC130 strain with the accession number KCCM12050P according to the present invention.

FIG. 2 shows the skin cell safety of a depsipeptide pseudoalteromonas strain according to one embodiment of the present invention.

FIG. 3 shows the effect of depsipeptide pseudoalteromonas strains on inhibiting melanin production according to one embodiment of the present invention.

FIG. 4 shows the effect of depsipeptide pseudoalteromonas strains on inhibiting melanogenesis enzyme activity according to one embodiment of the present invention.

Detailed Description

Hereinafter, the present invention will be described in detail.

In one aspect, the disclosed technology provides a composition for skin whitening comprising as active ingredients: a strain of the genus Pseudomonas peptaiized (Pseudomonas), a lysate thereof, a culture thereof, or an extract of said strain, a lysate thereof, or a culture thereof.

In another aspect, the presently disclosed technology provides a method of enhancing skin lightening, the method comprising: the method comprises the step of administering a pseudoalteromonas strain, a lysate thereof, a culture thereof, or an extract of the strain, the lysate thereof, or the culture thereof to a subject in need thereof at a dose effective to enhance skin whitening.

In another aspect, the disclosed technology provides a pseudoalteromonas strain, a lysate thereof, a culture thereof, or an extract of the strain, the lysate thereof, or the culture thereof for enhancing skin whitening.

In another aspect, the presently disclosed technology provides a non-therapeutic use of a strain of pseudoalteromonas, a lysate thereof, a culture thereof, or an extract of the strain, the lysate thereof, or the culture thereof, for enhancing skin whitening.

In another aspect, the disclosed technology provides a use of a pseudoalteromonas strain, a lysate thereof, a culture thereof, or an extract of the strain, the lysate thereof, or the culture thereof, in the preparation of a composition for enhancing skin whitening.

In another aspect, the disclosed technology provides a Pseudoalteromonas peptidolyticus (Pseudoalteromonas peptidolytica) SNC130 strain, the accession number of the Pseudoalteromonas peptidolyticus SNC130 being KCCM 12050P.

In an exemplary embodiment, the pseudoalteromonas strain, its lysate, its culture, or an extract of the strain, its lysate, or its culture may be applied, dosed, or spread to a subject in the form of a composition, for example, in the form of a skin external preparation or a cosmetic composition.

In an exemplary embodiment, the pseudoalteromonas strain, lysate thereof, culture thereof, or extract of the strain, lysate thereof, or culture thereof may be administered to the skin of a subject.

In an exemplary embodiment, the Pseudoalteromonas strain may be a peptide-decomposing Pseudoalteromonas (Pseudoalteromonas peptidolytica) strain.

In an exemplary embodiment, the strain may preferably be pseudoalteromonas depsipeptide SNC130 deposited under accession number KCCM 12050P.

In one exemplary embodiment, the strain may have a sequence comprising SEQ ID No.: 1, and a 16S rDNA having the base sequence shown in 1.

In one exemplary embodiment, the pseudoalteromonas peptidolyticus (pseudoalteromonas peptidolytica) SNC130 strain may have a skin whitening function.

In the present specification, "active ingredient" means an ingredient capable of exhibiting a desired activity by itself, or may exhibit a desired activity together with a carrier not having an activity of itself.

Unlike petroleum, water, and the like, microbial resources are advantageous in that they can be used as sustainable resources because they can be regenerated.

In an exemplary embodiment, the strain used may be prepared by: the strain is cultured and centrifuged to separate a culture solution, and then washed with sterilized physiological saline, dispersed in a solvent such as sterile oil, and then freeze-dried to prepare a lyophilized powder.

The lysate of the strain may refer to a product obtained by lysing the strain itself using chemical or physical force.

The culture of the strain may be a substance containing part or all of the substances contained in the medium in which the strain is cultured, regardless of the form of the culture, and for example, may refer to a substance containing metabolites or secretions that are culture products of the strain, or a lysate thereof, and the strain itself may also be contained in the culture.

In an exemplary embodiment, the culture may be obtained by culturing in a medium comprising one or more substances selected from the group consisting of starch, yeast extract, peptone, and sea salt.

The extract may refer to a product obtained by extracting, separating or fractionating the strain itself, a lysate of the strain, a culture of the strain, or a mixture thereof, regardless of the extraction method, the extraction solvent, the extracted components or the form of the extract, and is used in a broad concept including all substances that can be obtained by other processes or treatments after the extraction.

In an exemplary embodiment, the extract may preferably be an ethyl acetate fraction of a peptide-dissolving pseudoalteromonas culture.

In an exemplary embodiment, the pseudoalteromonas strain, its lysate, its culture, or an extract of the strain, its lysate, or its culture may be present in an amount of 0.001 wt% to 30 wt% based on the total weight of the composition. In another aspect, the pseudoalteromonas strain, lysate thereof, culture thereof, or extract of the strain, lysate thereof, or culture thereof may be present in an amount of 0.001 wt% or more, 0.01 wt% or more, 0.1 wt% or more, 0.5 wt% or more, 1 wt% or more, 1.5 wt% or more, or 2 wt% or more, and 30 wt% or less, 25 wt% or less, 20 wt% or less, 15 wt% or less, 10 wt% or less, or 5 wt% or less, based on the total weight of the composition.

The strain of the genus pseudoalteromonas, the lysate thereof, the culture thereof, or the extract thereof, or the composition for skin whitening containing the same according to the present invention has the effect of preventing, improving and/or treating symptoms or diseases caused by excessive melanin production, because it can effectively inhibit melanin production.

In an exemplary embodiment, the symptom or disease caused by melanin overproduction may be selected from dark spots (liver spots), freckles, age spots, Epidermal Melanocytic lesions (epiretinal melanocytesis), light brown pigmented spots (Cafe's aut lenticulae), Becker's Nevus, Nevus Spilus (Nevus Spilus), lentigo (Lentigines), Dermal Melanocytic lesions (dermatatic Lentigines), Mongolian lentigo (Lentigines), Nevus taifanensis (Mongolian spot), Nevus taifanensis (Nevus of Ota), Acquired bilateral Nevus taifanensis (Acquired bipolar Lentigines of Ota-lichens), Lentigines israya (Nevus of Nevus), blue (blueness), Melanocytic Lentigines (melanotic Lentigines), junction Lentigines (Junctional Nevus), Compound Nevus (Congenital Nevus), Nevus Congenital Nevus (Compound Nevus), Nevus Congenital Nevus (Nevus Congenital Nevus) Dysplastic nevus (dyspeptic nevus), Melanoma (Melanoma), Lentigo maligna Melanoma (Lentigo maligna Melanoma), Superficial spreading Melanoma (superfacial spreading Melanoma), acrolentigo lentiginous Melanoma (acrellising melanosome), Nodular Melanoma (Nodular Melanoma), pigmented basal cell carcinoma (pimentisal cell carcinoma), dermal fibroma (dermatibromas), dermoid cyst (dermoid cysts), keloid (keloid), and keratoacanthoma (keratocanthamas).

In an exemplary embodiment, the pseudoalteromonas strain, its lysate, its culture, or an extract of the strain, its lysate, or its culture, or a composition containing the same can prevent, ameliorate, and/or treat skin pigmentation.

In an exemplary embodiment, the pseudoalteromonas strain, the lysate thereof, the culture thereof, or the extract of the strain, the lysate thereof, or the culture thereof, or the composition containing the same may prevent, improve and/or treat one or more skin pigmentation selected from the group consisting of spots, freckles, lentigo, nevi, melanoma, drug-induced pigmentation, inflammation-induced pigmentation, and dermatitis-induced pigmentation.

According to an exemplary embodiment, the composition may be a skin external preparation composition.

In an exemplary embodiment, the composition for external preparation for skin may further comprise a preservative, a stabilizer, a wettable powder or an emulsifier, a pharmaceutical adjuvant for adjusting osmotic pressure such as a salt and/or a buffer, and other therapeutically useful substances in addition to the effective ingredient according to the present invention, and may be formulated into various parenteral dosage forms according to conventional methods

In one exemplary embodiment, the parenteral dosage form may be a transdermal dosage form. For example, the composition may be in the form of injection, ointment, lotion, gel, cream, spray, suspension, emulsion, patch, etc., but is not limited thereto.

In one exemplary embodiment, the skin external preparation composition may be a topical application agent having a pharmaceutical use for a melanin production-related disease.

According to an exemplary embodiment, the composition may be a cosmetic composition.

In one exemplary embodiment, the cosmetic composition may further include functional additives and ingredients generally included in cosmetic compositions, in addition to the active ingredient according to the present invention. The functional additive may include one or more of water-soluble vitamins, oil-soluble vitamins, polypeptides, polysaccharides, sphingolipids, and seaweed extracts. In addition, the cosmetic composition may further contain ingredients such as oil and fat ingredients, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, ethanol, blood circulation enhancers, refrigerants, antiperspirants, purified water, and the like.

The formulation of the cosmetic composition is not particularly limited and may be appropriately selected according to the purpose. For example, any one or more formulations such as, but not limited to, skin lotion, skin softening water, skin toning water, astringent water, milky lotion, moisturizing lotion, massage cream, nourishing cream, moisturizing cream, hand cream, foundation, essence, nourishing essence, mask, soap, cleansing foam, cleansing milk, cleansing cream, body lotion, and body wash may be prepared.

In an exemplary embodiment, when the dosage form according to the present invention is paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc or zinc oxide, etc. may be used as a carrier ingredient.

In an exemplary embodiment, when the formulation according to the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier ingredient, and particularly when the formulation is a spray, a propellant such as chlorofluorocarbon, propane/butane or dimethyl ether may be further included.

In an exemplary embodiment, when the dosage form according to the present invention is a solution or emulsion, a vehicle, solvent or opacifier may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butylene glycol oil, glycerol fatty acid ester, polyethylene glycol or fatty acid ester of sorbitan.

In an exemplary embodiment, when the dosage form according to the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, or the like, may be used as a carrier ingredient.

In an exemplary embodiment, when the formulation according to the present invention is a detergent containing a surfactant, sodium fatty alcohol sulfate, sodium fatty alcohol ether sulfate, sulfosuccinic acid monoester, sodium isethionate, imidazolidine derivative, sodium methyltaurate, sodium sarcosinate, sodium fatty acid amide ether sulfate, alkylamide betaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolenic acid derivative, or ethoxylated glycerol fatty acid ester may be used as a carrier component.