阅读说明：本技术 一种抗衰组合物及其制备方法和应用 (Anti-aging composition and preparation method and application thereof ) 是由 招敏聪 郭文姣 蔡慧珍 贺锐 杨悬 于 2020-07-13 设计创作，主要内容包括：本发明公开了一种抗衰组合物及其制备方法和应用。本发明的抗衰组合物包括如下质量份数的组分：松茸提取物5～20份、长裙竹荪提取物0.5～10份、桦褐孔菌提取物0.5～10份。通过松茸提取物、长裙竹荪提取物和桦褐孔菌提取物的协同作用,清除ABTS自由基的IC<Sub>50</Sub>值可以降低到0.52％,清除DPPH自由基的IC<Sub>50</Sub>值可以降低到0.46％可有效地清除DPPH自由基和ABTS自由基,有效地抵抗自由基的生成。而且,本发明的抗衰组合物且抑制酪氨酸酶的IC<Sub>50</Sub>值可以降低到2.45％,抑制弹性蛋白酶的IC<Sub>50</Sub>值可以降低到0.16％,可以很好的抑制酪氨酸酶并且促进胶原蛋白的生成,从而达到去皱、均匀肤色、美白、抗衰老的功效。(The invention discloses an anti-aging composition and a preparation method and application thereof. The anti-aging composition comprises the following components in parts by mass: 5-20 parts of tricholoma matsutake extract, 0.5-10 parts of dictyophora indusiata extract and 0.5-10 parts of inonotus obliquus extract. IC for eliminating ABTS free radical by synergistic action of matsutake extract, dictyophora indusiata extract and inonotus obliquus extract 50 The value can be reduced to 0.52%, and the IC for scavenging DPPH free radicals 50 The value can be reduced to 0.46%, which can effectively eliminate DPPH free radical and ABTS free radical, and effectively resist the generation of free radical. Furthermore, the anti-aging composition of the present invention inhibits tyrosinase IC 50 The value can be reduced to 2.45%, IC for inhibiting elastase 50 The value can be reduced to 0.16%, and the tyrosinase can be well inhibited and the collagen can be promotedThus achieving the effects of removing wrinkles, homogenizing skin color, whitening and resisting aging.)

1. The anti-aging composition is characterized by comprising the following components in parts by mass: 5-20 parts of tricholoma matsutake extract, 0.5-10 parts of dictyophora indusiata extract and 0.5-10 parts of inonotus obliquus extract.

2. The anti-aging composition as claimed in claim 1, which comprises the following components in parts by mass: 10-20 parts of tricholoma matsutake extract, 2-10 parts of dictyophora indusiata extract and 1-10 parts of inonotus obliquus extract.

3. The anti-aging composition as claimed in claim 2, which comprises the following components in parts by mass: 10 parts of tricholoma matsutake extract, 2 parts of dictyophora indusiata extract and 1 part of inonotus obliquus extract.

4. A method for preparing the antiaging composition as claimed in any one of claims 1 to 3, characterized by comprising the steps of:

s1, soaking matsutake, dictyophora indusiata and inonotus obliquus in water, extracting and separating under normal pressure to obtain supernatant, decoloring, and distilling under reduced pressure to obtain an extract;

s2, adding ethanol into the extract, mixing, standing, centrifuging, separating to obtain a precipitate, namely the anti-aging composition,

wherein the extraction temperature in S1 is 50-80 ℃, the extraction time is 2-5 h, and the reduced pressure distillation temperature is 45-75 ℃.

5. The preparation method of the anti-aging composition as claimed in claim 4, wherein the ratio of the water-soaked material to the water-soaked material in S1 is 1: 10-20.

6. The preparation method of the anti-aging composition as claimed in claim 4, wherein the mass ratio of the extract in S2 to ethanol is 1: 1-5.

7. Use of the anti-aging composition of any one of claims 1 to 3 in the preparation of an anti-aging skin care product.

8. An anti-aging skin care product, which is characterized by being prepared from the anti-aging composition as claimed in any one of claims 1 to 3 and acceptable auxiliary materials.

9. The anti-aging skin care product according to claim 8, wherein the anti-aging composition is contained in the anti-aging skin care product in an amount of 1 to 10% by mass.

10. The anti-aging skin care product according to claim 9, wherein the anti-aging composition is contained in the anti-aging skin care product in an amount of 5 to 10% by mass.

Technical Field

The invention relates to the technical field of cosmetics, and particularly relates to an anti-aging composition and a preparation method and application thereof.

Background

The skin is located on the body surface and is the most significant part of the body's aging process. The aged skin undergoes significant changes in both epidermal and dermal structure, slowing down epidermal cell renewal, weakening barrier function, decreasing keratinocyte viability, weakening repair capacity after epidermal injury, wherein weakening of barrier function results in skin dryness, desquamation, wrinkles, etc.; the alteration of the structure of the dermis is the main cause of skin aging, the thickness of the aged skin becomes thinner, and the ability to synthesize collagen and elastin is reduced. The number of melanocytes in aging skin is obviously reduced, the skin is easily injured when exposed to sunlight, lipofuscin is obviously increased, and senile plaques and other local pigment changes are shown; the aged skin sebaceous glands are atrophied in sweat glands, the secretion is reduced, the sweating reaction is reduced, the skin surface emulsion is insufficient, the hydration capability of the stratum corneum is weakened, and the skin is dry and cracked. With the increasing aging degree of society and the pursuit of people for youth, the share of anti-aging skin care products in the cosmetic market is gradually increased, and it is very necessary to provide a mild and effective anti-aging product to meet the requirements of consumers on the safety and efficacy of anti-aging cosmetics.

Fungi are known to be a large family of organisms, and the fruiting bodies of some wild and culturable mushrooms contain medicinal compounds for traditional medicine and cosmetics. During their evolution, fungi have evolved various properties to combat environmental stresses, and it is known that some fungal compounds may have the effect of modulating damage to organisms caused by the living and natural environment, thus enhancing the search for new natural bioactive compounds from fungal sources. The main component of the extract is polysaccharide which is an indispensable essential part in life metabolic activities and can be widely involved in various activities of human bodies, such as anti-aging, whitening and skin moisturizing. Polysaccharides extracted from animals and plants have the advantages of being natural, safe, non-irritant, high in skin affinity and the like, so that the application of the polysaccharides in cosmetics is widely concerned by people. At present, polysaccharide researches mainly comprise plant polysaccharide, animal polysaccharide, algae polysaccharide and the like, wherein the fungal polysaccharide researches are less. CN102895172A discloses a matsutake extract with the efficacy of resisting senility, preserving moisture and promoting penetration, a preparation method and application thereof, and discloses an extraction method of matsutake polysaccharide and application thereof in skin care products. However, the synergistic effect of the tricholoma matsutake and other fungus extracts is still to be further researched, and the anti-aging performance of the single tricholoma matsutake polysaccharide is also to be further improved.

Disclosure of Invention

The invention aims to overcome the defects and shortcomings of the anti-aging performance of the existing anti-aging product, and provides an anti-aging composition which can effectively remove free radicals, inhibit tyrosinase and promote the generation of collagen, thereby achieving the effects of skin color uniformity, whitening and anti-aging.

Another object of the present invention is to provide a method for preparing an anti-aging composition.

The invention further aims to provide application of the anti-aging composition in preparing anti-aging skin care products.

It is yet another object of the present invention to provide an anti-aging skin care product.

The above purpose of the invention is realized by the following technical scheme:

an anti-aging composition comprises the following components in parts by mass: 5-20 parts of tricholoma matsutake extract, 0.5-10 parts of dictyophora indusiata extract and 0.5-10 parts of inonotus obliquus extract.

The anti-aging composition can effectively remove DPPH free radicals and ABTS free radicals, effectively resist the generation of free radicals, inhibit tyrosinase, and promote the generation of collagen through the synergistic effect of the tricholoma matsutake extract, the dictyophora indusiata extract and the inonotus obliquus extract, so that the effects of uniform skin color, whitening and anti-aging are achieved.

Wherein:

the extract of the tricholoma matsutake contains 18 amino acids, 14 essential trace elements for human bodies, 49 active nutrient substances, 5 unsaturated fatty acids, 8 vitamins, 2 glycoproteins, abundant dietary fibers and a plurality of active enzymes, wherein the polysaccharide, the polypeptide, the steroid, the terpene, the grease, the plurality of enzymes and other substances have remarkable skin care activity, and the tricholoma matsutake polysaccharide and the polypeptide have more targeting property in the aspect of skin care, and have the effects of whitening, moisturizing, resisting aging and the like.

The tricholoma matsutake polysaccharide has good antioxidation, can remove superoxide anion free radicals, DPPH free radicals and hydroxyl free radicals through antioxidation, inhibit or block lipid peroxidation initiated by the free radicals, enhance the antioxidation activity of SOD, CAT and GSH-Px, inhibit MDA level from rising, reduce the peroxidation degree of tissue cells, enhance the immunologic function and delay aging. The tricholoma matsutake polysaccharide and the tricholoma matsutake triterpene have good tyrosinase inhibiting activity, wherein the polysaccharide TMSP-6II is reported to have better melanin generation inhibiting capability than arbutin. The tricholoma matsutake polysaccharide can well inhibit the problems of heavy pigment and uneven skin color caused by aging, the polysaccharide TMSP active ingredient can make the skin white and bright and convert the existing melanin into pale melanin, and meanwhile, the formation of the pale melanin can be further adjusted, so that the melanin is not formed any more, and the effects of whitening and anti-aging are achieved.

The dictyophora indusiata extract is rich in various active ingredients such as polysaccharide, protein, amino acid, vitamin, inorganic salt and the like, and has excellent effects of whitening, resisting oxidation, delaying senescence, inhibiting bacteria and the like. The dictyophora indusiata contains various amino acids, has good nutrition and moistening effects on skin, not only can obviously improve the immunity of an organism, but also can enable oxygenated hemoglobin in blood to release more oxygen to supply tissue cells, and has obvious improvement effect on melanin sediment in the skin.

Inonotus obliquus contains polysaccharide, inonotus obliquus alcohol, superoxide dismutase (SOD) and various triterpenoids. The superoxide dismutase (SOD) of the inonotus obliquus is 55 times of that of the ganoderma lucidum, 23 times of that of the agaricus blazei and 25 times of that of the hericium erinaceus, and the superoxide dismutase (SOD) is a key element for protecting cells against ROS. And the water-soluble polysaccharide such as 1, 3 beta glucan and 1, 6 beta glucan is as high as 55.6 mg/g.

The Inonotus obliquus extract can scavenge free radicals in vivo, protect cells, prolong division algebra of passage cells, prolong cell life, promote metabolism, effectively delay aging, improve and prevent allergic constitution, can be used as effective antioxidant and antiinflammatory, can act on differentiation (involucrin) and epidermal cohesion (integrin-B1, tight junction protein 1, and occludin) of cells, stimulate synthesis of collagen IV of basal layer and vascular wall structure, thereby strengthening skin barrier, enhancing epidermal cohesion, protecting and relieving skin, and can be used as effective antioxidant and antiinflammatory agent.

Preferably, the tricholoma matsutake extract is 10-20 parts, the dictyophora indusiata extract is 2-10 parts, and the inonotus obliquus extract is 1-10 parts.

More preferably, the composition comprises the following components in parts by mass: 10 parts of tricholoma matsutake extract, 2 parts of dictyophora indusiata extract and 1 part of inonotus obliquus extract.

The invention also provides a preparation method of the anti-aging composition, which comprises the following steps:

s1, soaking matsutake, dictyophora indusiata and inonotus obliquus in water, extracting and separating under normal pressure to obtain supernatant, decoloring, and distilling under reduced pressure to obtain an extract;

s2, adding ethanol into the extract, mixing, standing, centrifuging, separating to obtain a precipitate, namely the anti-aging composition,

wherein the extraction temperature in S1 is 50-80 ℃, the extraction time is 2-5 h, and the reduced pressure distillation temperature is 45-75 ℃.

Preferably, the ratio of the water to the soaking solution in S1 is 1: 10-20, for example, 1:10, 1:15 or 1:20, and more preferably 1: 10.

Preferably, the mass ratio of the extract to the ethanol in the S2 is 1: 1-5, for example, 1:1, 1:3 or 1: 5.

More preferably, the mass ratio of the extract to the ethanol in the S2 is 1: 3.

With the rise of the temperature of the water bath, the molecular heat movement is accelerated, the polysaccharide yield is increased, and when the temperature reaches more than 80 ℃, the polysaccharide content is increased slowly. Because the polysaccharide is an active substance, the structure of the polysaccharide can be damaged by overhigh temperature, the biological activity of the polysaccharide is influenced, and the subsequent experiment is adversely affected, so the extraction temperature is preferably 50-80 ℃.

Different feed-to-liquid ratios affect the dissolution of the active substance by the extraction solvent. The ratio of the feed liquid is low, and active substances cannot be fully dissolved, so that the extraction rate is influenced. If the ratio of the feed liquid is too high, the concentration of the active substance is low, and the subsequent purification and concentration steps are increased.

The application of the anti-aging composition in the preparation of anti-aging skin care products is also within the protection scope of the invention.

The anti-aging composition disclosed by the invention can effectively remove free radicals, inhibit tyrosinase and promote the generation of collagen, has excellent moisturizing, whitening and anti-aging effects, and can be widely applied to the preparation of anti-aging skin care products.

The invention also discloses an anti-aging skin care product which is prepared from the anti-aging composition and acceptable auxiliary materials.

Preferably, the anti-aging composition in the anti-aging skin care product is 1-10% by mass.

Preferably, the anti-aging composition in the anti-aging skin care product is 5-10% by mass.

Compared with the prior art, the invention has the beneficial effects that:

the invention provides an anti-aging composition, which is an IC for eliminating ABTS free radicals through the synergistic effect of tricholoma matsutake extract, dictyophora indusiata extract and inonotus obliquus extract₅₀The value can be reduced to 0.52%, and the IC for scavenging DPPH free radicals₅₀The value can be reduced to 0.46%, which can effectively eliminate DPPH free radical and ABTS free radical, and effectively resist the generation of free radical. Furthermore, the anti-aging composition of the present invention inhibits tyrosinase IC₅₀The value can be reduced to 2.45%, IC for inhibiting elastase₅₀The value can be reduced to 0.16%, tyrosinase can be well inhibited, and the generation of collagen can be promoted, so that the effects of skin color uniformity, whitening and aging resistance are achieved.

Drawings

FIG. 1 is a photograph showing the wrinkle removing effect before and after the sample is used by the volunteer

FIG. 2 is a photograph showing the skin whitening and skin tone evening effects before and after the sample is used by the volunteers

Detailed Description

The present invention will be further described with reference to specific embodiments, but the present invention is not limited to the examples in any way. The starting reagents employed in the examples of the present invention are, unless otherwise specified, those that are conventionally purchased.