阅读说明：本技术 一种油浆沉降剂 (Oil slurry settling agent ) 是由 林炯 于 2020-05-31 设计创作，主要内容包括：本发明公开了一种油浆沉降剂,其由嗜热脱氮芽孢杆菌菌株CGMCC-1228和假单胞菌CCTCC No:M 2012281共发酵获得的发酵提取液制备而成。本发明的油浆沉降剂由生物发酵制备得到,天然环保。本发明的油浆沉降剂沉降催化剂效果好,并且还能显著改善柴油的雾化性能,进一步改善油品的品质。(The invention discloses an oil slurry settling agent, which is prepared by fermentation extract obtained by co-fermentation of a bacillus denitrificans strain CGMCC-1228 and pseudomonas CCTCC No. M2012281. The oil slurry settling agent is prepared by biological fermentation, and is natural and environment-friendly. The oil slurry settling agent has good settling catalyst effect, and can also obviously improve the atomization performance of diesel oil and further improve the quality of oil products.)

1. An oil slurry settling agent is characterized in that:

it is prepared from fermentation extract obtained by co-fermenting Bacillus denitrificans strain CGMCC-1228 and pseudomonas CCTCC No. M2012281.

2. A process for the preparation of an oil slurry settling agent according to claim 1, characterized by the following:

(1) respectively activating strains:

respectively activating the strain CGMCC-1228 of the Geobacillus thermodenitrificans and the Pseudomonas CCTCC No. M2012281 in a glycerol cryopreservation tube;

(2) co-fermentation:

the strain CGMCC-1228 of the Geobacillus thermodenitrificans and the CCTCC No. M20122813 percent of the pseudomonas are co-fermented;

(3) preparing a fermentation extracting solution:

centrifuging the fermentation culture at 1200rpm, collecting supernatant, sterilizing with ultraviolet rays, filtering with 200 mesh filter to obtain slightly viscous fermentation extractive solution, and evaporating to concentrate to 1/2;

(4) adding tween80 in an amount of 0.5 part by weight of the fermentation extract to obtain an oil slurry sedimentation agent.

3. The method of claim 1, wherein:

respectively activating the strains in the step (1):

the strain CGMCC-1228 of Geobacillus thermodenitrificans in a glycerol cryopreservation tube is inoculated into a 100mL LB shake flask by using an inoculating and picking ring 3, and is subjected to shaking culture for 24 hours at 65 ℃ and 120 rpm.

4. The method of claim 3, wherein:

the step (2) is implemented by co-fermentation:

inoculating Bacillus denitrificans strain CGMCC-12283% and Pseudomonas CCTCC No. M20122813% in a liquid fermentation culture medium, and performing shake culture at 35 deg.C for 30 hr to obtain a fermentation culture with certain viscosity;

the formula of the liquid fermentation medium is as follows: according to weight percentage, 3 percent of glucose, 2 percent of maltose, 1 percent of peptone, 1 percent of NaCl and the balance of purified water, and the pH value is 7.0.

5. An oil slurry settling agent prepared by the method of claim 5.

6. Use of the slurry settling agent of claim 1 or 5 in the field of petroleum.

Technical Field

The invention relates to an oil slurry settling agent and a preparation method thereof, belongs to the field of petroleum, and particularly relates to an environment-friendly oil slurry settling agent prepared by a biotechnology.

Background

The catalytic slurry oil is a medium oil product from the bottom of a main fractionating tower of an FCC unit, and contains a large amount of fused ring aromatic hydrocarbon and a small amount of solid particles, wherein the most important part is silicon-aluminum catalyst powder, and impurities and corrosion products are also contained. The oil slurry is the lowest utilization value in FCC products, the catalytic oil slurry is generally sold as heavy fuel oil with lower sale price according to the current market condition, and the catalytic oil slurry can be used as asphalt raw material, coking raw material and boiler fuel of a thermal power plant, so that the adhesion of asphalt can be improved when the catalytic oil slurry is used as the asphalt raw material, but the method has lower utilization value, and the only beneficial value is used as the coking raw material and the boiler fuel, and the oil slurry contains catalyst powder, so the method has the following problems: 1. when the combustion is carried out, the nozzle of the combustor can be blocked, the ash drawing workload after the combustion is increased, and the normal operation and the heat efficiency of the heating furnace are influenced when the deposition is serious on the surface of the furnace tube. 2. The oil leakage phenomenon of the device can be caused when the pipe wall and the control valve of the device are seriously abraded, and the quality and the sale price of petroleum coke can be influenced. Therefore, the catalyst slurry should be used to remove the catalyst powder from the slurry as much as possible.

The oil slurry produced by catalytic cracking contains about catalyst particles. After the fuel oil blended by catalytic cracking slurry oil containing a large amount of catalyst solid particles is used, a large amount of catalyst solid powder is quickly deposited on a boiler furnace and a boiler tube, so that the heat transfer coefficient is greatly reduced, and in order to reach a preset temperature, the temperature of the furnace must be increased, so that not only is the oil consumption increased, but also the potential safety hazard of a boiler is increased. These solid particles of catalyst also have a negative effect on the erosion of pipes and equipment, further clogging the furnace mouth and causing damage. Once the diesel engine is worn, a cylinder may be pulled and stopped, and other destructive accidents may occur. In a more serious situation, due to abrasion, a ship sailing on the sea loses power and becomes a free floating island, and the ship cannot avoid danger when encountering storms, so that a serious accident of ship damage and death is possibly caused.

The sedimentation function of the oil slurry sedimentation agent is a physical and chemical process. Usually, the solid impurities and ash are gathered together by polar charges, the surface tension of the oil is overcome, and the solid impurities and the ash quickly sink to the bottom of an oil tank, so that the precipitation rate of the catalyst in the suspended oil slurry is accelerated. It has the following functions: (1) the electricity eliminating function: the electric double layer is destroyed and compressed, the counter-ionic phase is reduced and eliminated, and the thermal movement of the catalyst particles overcomes the electrostatic repulsion between the particles to cause coalescence. (2) Flocculation function: because the charge of the catalyst particles is eliminated, the particles are released from the active ingredients of the oil slurry, the interface affinity of the settling agent and the particles is stronger, and the flocculation of the particles is possible.

The invention aims to provide an environment-friendly oil slurry settling agent prepared by utilizing a biotechnology.

Disclosure of Invention

The invention aims to provide the environment-friendly oil slurry settling agent prepared by the biotechnology, the oil slurry settling agent has good settling effect, the preparation process is environment-friendly, and the spraying performance of diesel can be improved.

Microbial fermentation refers to a process of converting raw materials into products required by human beings through a specific metabolic pathway by using microorganisms under appropriate conditions. The level of microbial fermentation production depends mainly on the genetic characteristics of the strain itself and the culture conditions. The application range of fermentation engineering is medical industry, food industry, energy industry, chemical industry and agriculture: transforming plant genes; biological nitrogen fixation; engineering an insecticidal bacterium biopesticide; a microbial nutrient. Environmental protection and the like.

The invention group of the company applies the microorganisms to the production of the oil slurry settling agent for the first time.

Chinese patent 200410072759 discloses a strain of Geobacillus thermodenitrificans, which is characterized in that the preservation number of the strain in China general microbiological culture Collection center is CGMCC-1228. The strain can utilize crude oil as a unique carbon source, can improve the property of the crude oil, has the property of reducing the viscosity of the crude oil, improves the flow property of petroleum, and has the function of reducing the surface tension of substances, so the strain can be applied to the industry of preparing surfactants.

The research and development team of the company finds that the fermentation extract of the strain has the effect of an oil slurry settling agent, but the effect is not good enough. In further research, the thermophilic denitrified bacillus strain CGMCC-1228 and other strains are co-fermented, so that the effect of a fermentation extract of the thermophilic denitrified bacillus strain CGMCC-1228 serving as an oil slurry settling agent can be remarkably improved, the effect of improving the spraying performance of diesel oil is achieved, and the thermophilic denitrified bacillus strain CGMCC-1228 can be used as a diesel oil improver.

In the screening process of co-fermentation strains, a plurality of strains are found to have weak promotion effects, but the effects are not obvious, but one strain of pseudomonas has a very obvious sedimentation effect of promoting a fermentation extract of the pseudomonas to serve as an oil slurry sedimentation agent, and can bring a very obvious effect of improving the spraying performance of diesel oil. The strain of pseudomonas is disclosed by Chinese patent 201210398352 and is characterized in that: the preservation name is: pseudomonas stutzeri SC221-M, depository: china center for type culture Collection, collection address: wuhan university in Wuhan, China; the preservation date is as follows: day 7/11 2012, accession number: CCTCC No. M2012281.

In order to achieve the purpose, the invention adopts the following technical scheme:

an oil slurry settling agent is prepared by the following steps:

(1) respectively activating strains:

inoculating and selecting 3 rings of a strain CGMCC-1228 of the Geobacillus thermodenitrificans in a glycerol cryopreservation tube to a 100mLLB shake flask, and performing shake culture at 65 ℃ and 120rpm for 24 hours to obtain a seed solution;

inoculating and selecting 3 rings of pseudomonas CCTCC No. M2012281 in a glycerol freezing tube to a 100mL LB shaking bottle, and carrying out shaking culture for 24h at 32 ℃ and 100rpm to obtain a seed solution;

(2) co-fermentation:

inoculating the strain CGMCC-1228 seed solution of the Geobacillus thermodenitrificans obtained in the step (1) according to the inoculation amount of the Geobacillus thermodenitrificans strain CGMCC-12283%, inoculating the strain CCTCC No. M2012281 seed solution of the Geobacillus thermodenitrificans strain CCTCC No. M20122813% obtained in the step (1) into a liquid fermentation culture medium, and performing shaking culture at 35 ℃ for 30 hours to obtain a fermentation culture with certain viscosity;

the formula of the liquid fermentation medium is as follows: according to the weight percentage, 3 percent of glucose, 2 percent of maltose, 1 percent of peptone, 1 percent of NaCl and the balance of purified water, and the pH value is 7.0;

(3) preparing a fermentation extracting solution:

centrifuging the fermentation culture at 1200rpm for 20min, collecting supernatant, sterilizing with ultraviolet rays, filtering with 200 mesh filter to obtain slightly viscous fermentation extractive solution, and evaporating to concentrate to 1/2;

(4) adding tween80 in an amount of 0.5 parts by weight of the fermentation extract, and mixing to obtain the oil slurry settling agent.

Considering the convenience of subsequent purification, soluble sugar and necessary protein source (peptone) are used as energy sources as much as possible in the fermentation stage, so that the impurities in the fermentation supernatant can be reduced as little as possible, and the quality of the oil slurry settling agent is improved.

Compared with the prior art, the invention has the beneficial effects that:

(1) the oil slurry settling agent is prepared by biological fermentation, and is natural and environment-friendly.

(2) The oil slurry settling agent has good settling catalyst effect, and can also obviously improve the atomization performance of diesel oil and further improve the quality of oil products.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.