阅读说明：本技术 样本分析仪异常的报警方法、系统及存储介质 (Sample analyzer abnormity warning method, system and storage medium ) 是由 祁欢 郑文波 叶波 李朝阳 叶燚 于 2019-04-26 设计创作，主要内容包括：一种样本分析仪出现异常的报警方法、报警血小板检测异常和/或阻抗检测部件异常的系统及存储介质,样本分析仪出现异常的报警方法包括：将血液样本的第一部分与稀释液混合得到第一被测试样(S220)；将血液样本的第二部分与溶解试剂混合得到第二被测试样(S225)；检测第一被测试样的电阻抗信号(S230)；检测第二被测试样的至少两种光学信号(S235)；基于电阻抗信号得到第一血小板检测数据(S250)；基于至少两种光学信号得到第二血小板检测数据(S255)；基于第一血小板检测数据和第二血小板检测数据之间的差异得到评估结果(S270)；判断评估结果是否满足预设条件(S280)以报警第一血小板检测异常和/或电阻抗信号检测出现异常(S290)。(A method for alarming abnormality of a sample analyzer, a system for alarming abnormality of platelet detection and/or abnormality of an impedance detection part, and a storage medium, the method for alarming abnormality of a sample analyzer includes: mixing a first portion of the blood sample with a diluent to obtain a first sample to be tested (S220); mixing a second portion of the blood sample with a lysing reagent to obtain a second test sample (S225); detecting an electrical impedance signal of the first test sample (S230); detecting at least two optical signals of a second test sample (S235); obtaining first platelet detection data based on the electrical impedance signal (S250); obtaining second platelet detection data based on the at least two optical signals (S255); obtaining an evaluation result based on a difference between the first platelet measurement data and the second platelet measurement data (S270); it is judged whether the evaluation result satisfies a preset condition (S280) to alarm the first platelet detection abnormality and/or the electrical impedance signal detection abnormality (S290).)

A method for alarming abnormality in a sample analyzer, the method comprising:

providing a blood sample;

mixing a first part of the blood sample with a diluent to obtain a first sample to be tested for first platelet detection;

mixing a second portion of said blood sample with a lytic reagent to provide a second test sample for a second platelet test, wherein red blood cells in the second test sample are lysed;

detecting an electrical impedance signal of the first sample under test;

detecting at least two optical signals of the second test sample;

obtaining first platelet detection data for the blood sample based on the electrical impedance signal;

obtaining second platelet detection data for the blood sample based on the at least two optical signals;

obtaining an assessment based on a difference between the first platelet detection data and the second platelet detection data;

judging whether the evaluation result meets a preset condition or not; and

and when the judgment result is yes, alarming that the first platelet detection is abnormal and/or the electrical impedance signal detection step of the sample analyzer is abnormal.

The alarm method of claim 1, wherein said deriving second platelet detection data for the blood sample based on the at least two optical signals comprises:

generating a scatter plot of the second test sample based on the at least two optical signals;

differentiating between white blood cell regions and platelet regions in the scatter plot based on the at least two optical signals;

second platelet detection data is obtained for the blood sample based on the platelet region.

The alarm method according to claim 1, wherein a prompt message indicating that the cause of the first platelet detection abnormality is an abnormality in the electrical impedance signal detection step and/or an unreliable first platelet detection result is output.

The alarm method of claim 1, wherein the lytic reagent comprises a hemolytic agent for lysing red blood cells and a fluorescent dye for staining blood cells, and the at least two optical signals comprise a forward scattered light signal and a fluorescent signal.

The alarm method of claim 1, wherein the lytic reagent comprises a hemolytic agent for lysing red blood cells, wherein the at least two optical signals comprise a first scattered light signal and a second scattered light signal, wherein the first scattered light signal is a forward scattered light signal, and wherein the second scattered light signal is at least one of a medium angle scattered light signal and a side scattered light signal.

The alarm method of claim 4 or 5, wherein the step of obtaining second platelet detection data of the blood sample based on the platelet region comprises:

deriving a derived platelet volume histogram based at least on the forward scattered light signals of the population of particles present in the platelet region; or

Second platelet detection data for the blood sample is obtained based on the number of particles present in the platelet region.

The alarm method of claim 1, wherein the lytic reagent comprises a hemolytic agent for lysing red blood cells and a fluorescent dye for staining blood cells, and the at least two optical signals comprise a side scattered light signal and a fluorescent signal; second platelet detection data for the blood sample is obtained based on the number of particles present in the platelet region.

The alarm method of any one of claims 2-7, wherein the platelet region comprises a large platelet region, and wherein the large platelet region is used to obtain second platelet detection data for the blood sample.

The alarm method according to any one of claims 1 to 8, wherein the first platelet detection data is at least one characteristic parameter of first platelet volume distribution data, and the second platelet detection data is at least one characteristic parameter of second platelet volume distribution data;

preferably, the characteristic parameter is selected from one or more of platelet count, platelet volume histogram, average platelet volume and platelet volume distribution width; or

Preferably, the characteristic parameter is selected from one or more of platelet count, platelet volume histogram, mean platelet volume and platelet volume distribution width within a certain volume threshold range.

The alarm method according to claim 1, wherein the two optical signals comprise a scattered light signal and a fluorescent signal, and the method further comprises differentiating the white blood cells into subpopulations of white blood cells, or counting or identifying nucleated red blood cells or immature cells or basophils, based on the scattered light signal and the fluorescent signal;

alternatively, the two optical signals comprise a first scattered light signal and a second scattered light signal, the first scattered light signal is a forward scattered light signal, the second scattered light signal is at least one of a medium angle scattered light signal and a side scattered light signal, and the method further distinguishes the white blood cells into subpopulations of white blood cells or identifies basophils according to the first scattered light signal and the second scattered light signal.

The alarm method according to claim 1, wherein the step of judging whether the evaluation result satisfies a preset condition comprises:

comparing the degree of pattern difference between the first platelet measurement data and the second platelet measurement data;

judging whether the figure difference degree meets a preset condition or not; or

Acquiring numerical information of the first platelet detection data and the second platelet detection data;

calculating an evaluation value using the numerical information, the evaluation value reflecting a degree of difference between the first platelet detection data and the second platelet detection data;

and judging whether the evaluation value meets a preset condition or not.

The alarm method according to claim 1, further comprising the steps of:

if no alarm is abnormal, outputting the first platelet detection data;

and if the alarm is abnormal, outputting the second platelet detection data.

The alarm method according to claim 1, wherein the judgment result of the platelet detection evaluation values obtained from a plurality of consecutive blood samples is recorded and counted, and when the judgment results of the plurality of consecutive blood samples are all yes, the alarm electrical impedance signal detection is abnormal.

A non-transitory computer-readable storage medium having a computer program stored thereon, wherein: the computer program realizes the steps of the alarm method according to any one of claims 1-13 when executed by a processor.

A blood analysis system, comprising:

a sample processing device comprising at least one mixing chamber for mixing a first portion of a blood sample with a diluent to produce a first test sample for a first platelet test; mixing a second portion of said blood sample with a lytic reagent to provide a second test sample for a second platelet test, wherein red blood cells in the second test sample are lysed;

a sample detection device comprising an electrical impedance detection component and an optical detection component, the electrical impedance detection component comprising a micro-well and an electrical impedance detector, the electrical impedance detector being configured to detect an electrical impedance signal of the first sample under test passing through the micro-well, the optical detection component comprising an optical flow chamber, a light source, and an optical detector, the optical flow chamber being in communication with the mixing chamber, the light source being configured to direct a light beam at the optical flow chamber, the optical detector being configured to detect at least two optical signals of the second sample under test passing through the optical flow chamber;

the data analysis module comprises a signal acquisition module, a classification counting module and an alarm module;

the signal acquisition module acquires the electrical impedance signal of the first tested sample, and the signal acquisition module acquires the at least two optical signals of the second tested sample;

the classification counting module obtains first platelet detection data of the blood sample based on the electrical impedance signal; the classification counting module generates a scatter diagram of the second test sample based on the at least two optical signals, distinguishes between a leukocyte region and a platelet region in the scatter diagram based on the at least two optical signals, and obtains second platelet detection data of the blood sample based on the platelet region;

the alarm module obtains an evaluation result based on a difference between the first platelet detection data and the second platelet detection data; judging whether the evaluation result meets a preset condition or not; and when the judgment result is yes, alarming that the first platelet detection is abnormal and/or the electrical impedance detection component is abnormal.

The blood analysis system of claim 15, wherein the classification count module comprises:

generating a scatter plot of the second test sample based on the at least two optical signals;

differentiating between white blood cell regions and platelet regions in the scatter plot based on the at least two optical signals;

second platelet detection data is obtained for the blood sample based on the platelet region.

A blood analysis system according to claim 15, wherein the alarm module includes a prompt to output a message that the cause of the first platelet detection abnormality is an abnormality in the electrical impedance detection component and/or an unreliable first platelet detection result.

A blood analysis system according to claim 15, wherein the lysing reagent comprises a lysing agent for lysing red blood cells and a fluorescent dye for staining blood cells, the at least two optical signals comprise forward scattered light signals and fluorescent signals, and the optical detection means comprises at least one scattered light detector and at least one fluorescent detector.

The blood analysis system of claim 15, wherein the lysing reagent comprises a lysing agent for lysing red blood cells, the at least two optical signals comprise a first scattered light signal and a second scattered light signal, the first scattered light signal is a forward scattered light signal, the second scattered light signal is at least one of a medium angle scattered light signal and a side scattered light signal, and the optical detection component comprises at least two scattered light detectors.

A blood analysis system according to claim 18 or 19, wherein the classification count module derives a derived platelet volume histogram based at least on the forward scattered light signals of the population of particles present in the platelet region; or

The classification counting module obtains second platelet detection data of the blood sample based on the number of particles present in the platelet region.

The blood analysis system of claim 15, wherein the lysing reagent comprises a lysing agent for lysing red blood cells and a fluorescent dye for staining blood cells, and the at least two optical signals comprise a side scattered light signal and a fluorescent signal; the classification module obtains second platelet detection data for the blood sample based on the number of particles present in the platelet region.

A blood analysis system according to claims 16-21, wherein the platelet region comprises a large platelet region and the second platelet measurement data comprises second large platelet data, the second platelet measurement data of the blood sample being obtained using the large platelet region.

A blood analysis system according to claims 15-22, wherein the first platelet detection data is at least one characteristic parameter of first platelet volume distribution data and the second platelet detection data is the at least one characteristic parameter of second platelet volume distribution data;

preferably, the characteristic parameter is selected from one or more of platelet count, platelet volume histogram, average platelet volume and platelet volume distribution width; or

Preferably, the characteristic parameter is selected from one or more of platelet count, platelet volume histogram, mean platelet volume and platelet volume distribution width within a certain volume threshold range.

The blood analysis system of claim 15, wherein the two optical signals comprise scattered light signals and fluorescence signals, and the differential counting module further distinguishes leukocytes into subpopulations of leukocytes, counts leukocytes, or identifies nucleated erythrocytes or immature cells or basophils based on the scattered light signals and fluorescence signals;

or the two optical signals comprise a first scattered light signal and a second scattered light signal, the first scattered light signal is a forward scattered light signal, the second scattered light signal is at least one of a medium-angle scattered light signal and a side scattered light signal, and the classification counting module is further used for distinguishing the white blood cells into the sub-groups of the white blood cells or identifying basophils according to the first scattered light signal and the second scattered light signal.

The blood analysis system of claim 15, wherein the alarm module comprises:

comparing the degree of pattern difference between the first platelet measurement data and the second platelet measurement data;

judging whether the figure difference degree meets a preset condition or not; or

Acquiring numerical information of the first platelet detection data and the second platelet detection data;

calculating an evaluation value using the numerical information, the evaluation value reflecting a degree of difference between the first platelet detection data and the second platelet detection data;

and judging whether the evaluation value meets a preset condition or not.

The blood analysis system of claim 15, further comprising a user interface:

if no alarm is abnormal, outputting the first platelet detection data;

and if the alarm is abnormal, outputting the second platelet detection data.