阅读说明：本技术 拟南芥ant基因在提高鸡冠花耐旱性和种子产量中的应用 (Application of arabidopsis ANT gene in improvement of cockscomb drought tolerance and seed yield ) 是由 孟来生 曹小迎 宛雯 于 2019-03-05 设计创作，主要内容包括：拟南芥ANT基因在提高鸡冠花耐旱性和种子产量中的应用,所述拟南芥ANT基因的核苷酸序列如SEQ ID No.1所示。本发明将拟南芥ANT基因应用于培育耐逆性鸡冠花良种及种子产量高的品种,通过在转基因鸡冠花中表达上述拟南芥ANT基因提高了鸡冠花的耐干旱性和种子的长度、宽度及重量,在鸡冠花育种技术领域具有较好的应用前景。(The application of the arabidopsis ANT gene in improving the drought tolerance and the seed yield of cockscomb is disclosed, and the nucleotide sequence of the arabidopsis ANT gene is shown as SEQ ID No. 1. According to the invention, the arabidopsis ANT gene is applied to cultivation of stress-tolerant cockscomb fine breeds and breeds with high seed yield, and the drought tolerance of cockscomb and the length, width and weight of seeds are improved by expressing the arabidopsis ANT gene in transgenic cockscomb, so that the arabidopsis ANT gene has a good application prospect in the technical field of cockscomb breeding.)

1. Application of Arabidopsis ANT gene in improving drought tolerance of cockscomb.

2. Application of Arabidopsis ANT gene in increasing the yield of cockscomb seeds.

3. Application of Arabidopsis ANT gene in preparing transgenic cockscomb is provided.

4. Application of Arabidopsis ANT gene in improving cockscomb germplasm resources.

5. A method for improving the quality of cockscomb, which comprises expressing the Arabidopsis ANT gene described in claim 1 in transgenic cockscomb.

Technical Field

The invention relates to the fields of molecular biology and genetic engineering, in particular to application of an arabidopsis ANT gene in improving the drought tolerance and the seed yield of cockscomb.

Background

Cockscomb is one of the most common ornamental plants in the world. The flower has golden, red, orange, yellow and pink colors. Cockscomb seed is a potential food product with a protein content of over 73%. The cockscomb is not only a common flower which is beautiful and lovely in summer and autumn, but also can be made into good medicines and delicacies, and has good body building effect. Cockscomb is used as medicine by flower and seed. The seed has effects of diminishing inflammation, astringing, improving eyesight, lowering blood pressure, and strengthening body, and can be used for treating hematochezia, red and white dysentery, metrorrhagia, stranguria with turbid urine, and eye diseases. When the cockscomb is subjected to drought stress in the cultivation process, a series of physiological changes such as light and efficiency reduction which are not beneficial to the normal growth of the cockscomb can be caused, and the symptoms such as growth inhibition, aging promotion and the like are shown. Improving the seed size and drought tolerance of the cockscomb has important significance for enhancing the stress tolerance of the cockscomb and increasing the seed yield.

Disclosure of Invention

As one aspect of the present invention, there is provided an application of an Arabidopsis ANT gene for improving drought tolerance of a cockscomb.

The invention also provides application of the arabidopsis ANT gene in increasing the yield of cockscomb seeds.

The invention also provides application of the arabidopsis ANT gene in preparation of transgenic cockscomb.

The invention also provides the application of the arabidopsis ANT gene in improving cockscomb germplasm resources.

The invention also provides a method for improving the quality of cockscomb, which expresses the arabidopsis ANT gene in transgenic cockscomb.

The invention has the beneficial effects that: the invention can greatly improve the drought resistance and seed yield of cockscomb by expressing the arabidopsis ANT gene in the transgenic cockscomb, can be used for cultivating stress-tolerant cockscomb improved varieties and varieties with high seed yield, and has better application prospect in cultivating cockscomb improved varieties.

Drawings

FIG. 1 shows the composition of the T-DNA region of pCB308R and the insertion positions of 35S and ANT;

FIG. 2 is a comparison of wild type and transgenic cockscomb plant morphology under normal conditions and drought stress;

FIG. 3 is a graph comparing the seed traits of wild type and transgenic cockscomb under normal conditions and drought stress.

The specific implementation mode is as follows:

the project embodiments provided by the present invention are illustrative of the present invention, but not limiting the scope of the present invention. The technical means in the practice of the invention are, unless otherwise indicated, techniques commonly employed in the research field, and the starting materials used are commercially available. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art.