阅读说明：本技术 一种包含香草硫缩病醚和乙蒜素的农药组合物及其用途 (Pesticide composition containing Vanilthioketal and ethylicin and application thereof ) 是由 王少龙 文香玲 邓秀丽 薛照先 张善学 赵华锋 王玲玲 于 2020-07-22 设计创作，主要内容包括：本发明涉及一种包含香草硫缩病醚和乙蒜素的农药组合物及其用途,所述农药组合物中主要活性成分为香草硫缩病醚和乙蒜素,两者的质量比为1:0.01-100。本发明的农药组合物中,香草硫缩病醚和乙蒜素在防治植物病毒病的过程中表现出协同增效的作用,能够有效降低生产成本和使用成本,达到农药减量增效的目的。(The invention relates to a pesticide composition containing Vanilothiopsis ether and ethylicin and application thereof, wherein the main active ingredients in the pesticide composition are Vanilothiopsis ether and ethylicin in a mass ratio of 1: 0.01-100. In the pesticide composition, the vanilla thioketal and the ethylicin show synergistic effect in the process of preventing and treating plant virus diseases, so that the production cost and the use cost can be effectively reduced, and the purposes of reducing and enhancing the pesticide amount are achieved.)

1. A pesticidal composition comprising a vanillyl thioketal and ethylicin, said vanillyl thioketal being a compound having the following formula (I):

wherein the mass ratio of the vanilla thioketal to the ethylicin is 1: 0.01-100.

2. The pesticidal composition according to claim 1, characterized in that the mass ratio of vanillyl thioketal to ethylicin is from 1:0.05 to 40, preferably the mass ratio of vanillyl thioketal to ethylicin is from 1:0.1 to 20.

3. The pesticide composition as set forth in claim 1, wherein the sum of the weights of the Vanillythide and the ethylicin accounts for 1-80% of the pesticide composition, preferably the sum of the weights of the Vanillythide and the ethylicin accounts for 20-50% of the pesticide composition, and preferably the sum of the weights of the Vanillythide and the ethylicin accounts for 22-42% of the pesticide composition.

4. The pesticidal composition according to claim 4, characterized in that the pesticidal composition is a formulation in the form of a soluble solution, a wettable powder, a water dispersible granule, an aqueous emulsion, a microemulsion, a suspension agent or an emulsifiable concentrate.

5. Use of the pesticidal composition according to any one of claims 1 to 4 for controlling a plant disease which is a plant bacterial disease, a plant fungal disease or a plant viral disease.

6. The use according to claim 5, characterized in that the plant viral disease is one or more of rice viral disease, tomato viral disease, cucumber mosaic viral disease, watermelon mosaic viral disease, pepper mosaic viral disease, citrus yellow vein viral disease, maize rough dwarf disease, apple defoliation, grape leaf roll disease, rice stripe virus disease, rice yellow dwarf virus disease, rice black-streaked dwarf virus disease, tomato yellow leaf curl virus disease, tomato spotted wilt virus disease, tomato chlorosis virus disease, citrus tristeza virus disease, citrus split skin virus disease, citrus leaf shatter virus disease, citrus chlorosis dwarf virus disease.

7. The method for controlling plant virus diseases by applying the pesticide composition as claimed in any one of claims 1 to 4, characterized in that the pesticide composition is sprayed at the initial stage of the occurrence of plant diseases at a rate of 100-4000 times for 2-3 times.

Technical Field

The invention belongs to the field of agricultural control medicaments, and particularly relates to a pesticide composition containing Vanilthioketal and ethylicin and application thereof.

Background

Plant virus diseases are diseases caused by plant virus parasitism, are second to fungal diseases in plant diseases, and are called plant cancers. In recent years, with the development of protected agriculture, the international agricultural product trade is continuously expanded, the occurrence range of crop virus diseases mainly transmitted by vector insects is continuously expanded, and the yield and the quality of crops are severely restricted. The plant virus has a large quantity and a wide range of damage, and the crop generally lacks of germplasm resources for resisting virus diseases, so that the difficulty in preventing and controlling the virus diseases is high. At present, chemical agents are still the main means for preventing and treating plant virus diseases in production, mainly comprise natural inhibitors and chemical synthesis inhibitors, the prevention and treatment effects of the chemical synthesis inhibitors on the plant virus diseases are not ideal, the environmental impact is large, the varieties of the natural inhibitors are few, and the requirements of modern agriculture on large scale and multiple varieties cannot be met, so that novel, efficient and environment-friendly virus disease prevention and treatment agents are urgently needed.

Vanillin is also known as vanillin, and is an important natural product with biological safety, simple structure and good biological activity. Vanillin has wide application in light industry and pharmaceutical chemical industry, can be added into food, toothpaste, soap and tobacco as main raw material for fixing fragrance, and can also be used for preparing common medicines for treating hypertension, heart disease and dermatosis, but the vanillin is applied in agricultural field for less research. In the prior art, patent CN106467478A discloses a dithioacetal-containing vanillin derivative, a preparation method and an application thereof, wherein the compound can inhibit various plant viruses such as tobacco mosaic virus, cucumber mosaic virus, potato virus Y, southern rice black-streaked dwarf virus, rice stripe virus and the like.

In 2018, Mengxinggang and the like (Salmin, Hodgy, Songbaoan, fluorescent two-photon confocal and UPLC-HRMS methods explore the internal absorption and conduction characteristics of Vanillythion in tobacco plants) report that a vanillin derivative, Vanillythion, has better upward and downward internal absorption and conduction characteristics in tobacco plants, and provide a certain reference for applying the Vanillythion to field tests.

Ethylicin is an ethyl homolog of allicin, is a high-efficiency pollution-free broad-spectrum bionic bactericide, has good prevention and control effects on various diseases, and has a plant growth regulating effect. Ethylicin reacts with the substance containing-SH group in the thallus molecule through the (S-S ═ O) group in the molecular structure, thereby inhibiting the normal metabolism of the thallus and achieving the effect of preventing and treating diseases. The inventor finds that the combination of the vanilla thioketal and the ethylicin has synergistic effect on preventing and treating plant virus diseases.

At present, no relevant report is found at home and abroad about the compound product of the vanilla thioketal and the ethylicin.

Disclosure of Invention

In order to solve the above technical problems, the present invention provides a pesticide composition comprising vanillyl thioketal and ethylicin, wherein the vanillyl thioketal is a compound having the following formula (I):

in the pesticide composition, the mass ratio of the Vanilthioketal to the ethylicin is 1: 0.01-100.

Preferably, in the pesticide composition, the mass ratio of the vanilla thioketal to the ethylicin is 1:0.05-40, and the mass ratio of the vanilla thioketal to the ethylicin is 1: 0.1-20.

Preferably, in the pesticide composition, the sum of the mass of the Vanilthioketal and the ethylicin accounts for 1 to 80 percent of the mass of the pesticide composition, preferably, the sum of the mass of the Vanilthioketal and the ethylicin accounts for 20 to 50 percent of the mass of the pesticide composition, and preferably, the sum of the mass of the Vanilthioketal and the ethylicin accounts for 22 to 42 percent of the mass of the pesticide composition.

Preferably, in the pesticide composition, the pesticide composition contains a pesticide carrier or a pesticide adjuvant, the pesticide carrier or the pesticide adjuvant accounts for 20-99% of the pesticide composition by mass, preferably, the pesticide carrier or the pesticide adjuvant accounts for 50-80% of the pesticide composition by mass, and preferably, the pesticide carrier or the pesticide adjuvant accounts for 58-78% of the pesticide composition by mass.

Preferably, in the pesticide composition, the pesticide composition is a preparation, and the preparation is soluble agent, wettable powder, water dispersible granules, emulsion in water, microemulsion, suspending agent or missible oil.

The invention also provides application of the pesticide composition in preventing and treating plant diseases, wherein the plant diseases are plant bacterial diseases, plant fungal diseases or plant virus diseases.

Preferably, in the above use, the plant disease is a plant bacterial disease or a plant viral disease.

Preferably, in the above use, the plant virus disease is one or more of mosaic virus disease, yellowing virus disease, malformation virus disease or necrosis virus disease which harm vegetables, fruit trees and field crops;

preferably, in the above uses, the virus disease is one or more of rice virus disease, tomato virus disease, cucumber mosaic virus disease, watermelon mosaic virus disease, pepper mosaic virus disease, citrus yellow vein virus disease, maize rough dwarf disease, apple defoliation disease, grape leaf roll disease, rice stripe disease, rice yellow dwarf virus disease, rice black-streaked dwarf virus disease, tomato yellow leaf curl virus disease, tomato spotted wilt virus disease, tomato chlorosis virus disease, citrus tristeza virus disease, citrus split skin virus disease, citrus leaf shattering virus disease and citrus chlorosis dwarf virus disease.

Particularly preferably, in the above application, the plant virus disease is one or more of rice virus disease, tomato virus disease, cucumber mosaic virus disease, watermelon mosaic virus disease and pepper mosaic virus disease.

The invention also provides a method for preventing and treating plant virus diseases by applying the pesticide composition, which comprises the step of spraying the pesticide composition at the initial stage of plant disease occurrence, wherein the application time is 100-4000 times, and the application frequency is 2-3 times.

The invention has the advantages of

In the pesticide composition, the vanilla thioketal and the ethylicin show synergistic effect in the process of preventing and treating plant virus diseases, so that the production cost and the use cost can be effectively reduced, and the purposes of reducing and enhancing the pesticide amount are achieved.

Detailed Description

Preparation of the formulation

The following preparations are all provided by the agro-gaco ltd in the south China Hainan Zhengye, part of the preparations are prepared by processing the preparations through raw medicines purchased in the market, and part of the preparations are prepared by adjusting the concentration of the preparations purchased in the market. The following examples are further illustrative of the present invention, but the present invention is not limited to the proportions, type of formulation and use in the examples. In the following examples, the complex composition of Vanilthioketal and ethylicin is prepared into emulsifiable concentrate, and the preparation method is a conventional emulsifiable concentrate preparation method. In each preparation, the proportion of the Vanillythion and the ethylicin is calculated according to the mass ratio. The following formulations of Vanilthioketal and ethylicin were prepared as required.

Preparation 1: 2% Vanilla thioketal 40% ethylicin emulsifiable concentrate (42% fragrant and ethylicin emulsifiable concentrate for short)

Preparation 2: 2% Vanilla thioketal 20% ethylicin emulsifiable concentrate (22% fragrant and ethylicin emulsifiable concentrate for short)

Preparation 3: 5% Vanilla thioketal and 30% ethylicin emulsifiable concentrate (35% fragrant and ethylicin emulsifiable concentrate for short)

Preparation 4: 5% Vanilla thioketal 20% ethylicin emulsifiable concentrate (25% fragrant and ethylicin emulsifiable concentrate for short)

Preparation 5: 10% Vanilla thioketal and 20% ethylicin missible oil (30% fragrant and ethylicin missible oil for short)

Preparation 6: 20% Vanilla thioketal 2% ethylicin emulsifiable concentrate (22% fragrant and ethylicin emulsifiable concentrate for short)

(II) application test

(1) Investigation method

Disease index base number is investigated before medicament treatment, leaf number and disease grade of each grade of disease are investigated according to investigation requirements after medicament treatment, and disease index and prevention and treatment effect are calculated. The pesticide effect investigation is carried out each time, and the occurrence of phytotoxicity (such as burn, withering, fallen leaves, fallen flowers, fallen fruits, poor growth, leaf deformity and the like) in each pesticide application area is observed.

(2) Disease grading standard

Grade 0, no symptoms;

grade 1, the lesion area accounts for less than 5% of the whole leaf area;

grade 3, the lesion area accounts for 6 to 15 percent of the whole leaf area;

grade 5, the lesion area accounts for 16-30% of the whole leaf area;

grade 7, the lesion area accounts for 31-50% of the whole leaf area;

grade 9, the lesion area accounts for more than 51% of the whole leaf area.

(3) The control effect (control effect) calculation method comprises the following steps:

(4) synergy verification method

The expected effect of a particular combination of two ingredients can be calculated using the Colby formula:

x is the control effect of crop diseases with the component A1 and the dosage B1;

y is the control effect of the crop diseases using the A2 component with the dosage of B2;

b1 and B2 may be the same or different.

E₀The theoretical control effect of preventing and controlling crop diseases is obtained by compounding the A1 component with the dosage of B1 and the A2 component with the dosage of B2.

E₁The A1 component with the dosage of B1 and the A2 component with the dosage of B2 are compounded to have actual control effect on crop diseases.

If E is₁＞E₀The compound of the component A1 and the component A2 is proved to be synergistic in controlling crop diseases.