阅读说明：本技术 药用沙棘果汁生产方法 (Production method of medicinal sea-buckthorn fruit juice ) 是由 田志杰 李彭帅 于海泉 于 2020-06-12 设计创作，主要内容包括：本公开涉及医药领域,尤其涉及一种药用沙棘果汁生产方法,包括以下步骤：(1)压榨沙棘果,分取沙棘原汁和果渣；(2)分离所述沙棘原汁,分取果泥、果油和清果汁；(3)将在步骤(1)中分取的所述果渣与在步骤(2)中分取的所述果油、果泥混合,形成果渣类混合物；用溶剂提取所述果渣类混合物中的黄酮类有效成分,并过滤提取液；然后除去滤液中的溶剂,获得含有黄酮类物质的浓缩液；(4)将在步骤(3)中获得的浓缩液与在步骤(2)中获得的清果汁依次进行充分混合、静置和精细分离,然后取分离出的沙棘汁清液进行浓缩,获得富含黄酮类物质的药用沙棘果汁。采用本公开方法制备的药用沙棘果汁载药量高且稳定性良好。(The invention relates to the field of medicines, in particular to a production method of medicinal sea-buckthorn juice, which comprises the following steps: (1) squeezing fructus Hippophae, and separating fructus Hippophae juice and fruit residue; (2) separating the raw juice of the sea buckthorn, and separating fruit paste, fruit oil and clear fruit juice; (3) mixing the pomace separated in step (1) with the fruit oil and fruit paste separated in step (2) to form a pomace mixture; extracting effective components of flavonoids in the pomace mixture with a solvent, and filtering the extract; then removing the solvent in the filtrate to obtain a concentrated solution containing flavonoids; (4) and (3) fully mixing the concentrated solution obtained in the step (3) and the clear juice obtained in the step (2) in sequence, standing, finely separating, and concentrating the separated clear sea-buckthorn juice to obtain the medicinal sea-buckthorn juice rich in flavonoids. The medicinal seabuckthorn fruit juice prepared by the method has high drug loading and good stability.)

1. A production method of medicinal sea-buckthorn juice is characterized by comprising the following steps:

(1) squeezing fructus Hippophae, and separating fructus Hippophae juice and fruit residue;

(2) separating the raw juice of the sea buckthorn, and separating fruit paste, fruit oil and clear fruit juice;

(3) mixing the pomace separated in step (1) with the fruit oil and fruit paste separated in step (2) to form a pomace mixture; extracting effective components of flavonoids in the pomace mixture with a solvent, and filtering the extract; then removing the solvent in the filtrate to obtain a concentrated solution containing flavonoids;

(4) and (3) fully mixing the concentrated solution obtained in the step (3) and the clear fruit juice obtained in the step (2) in sequence, standing, finely separating, and concentrating the separated clear liquid of the seabuckthorn fruit juice to obtain the seabuckthorn fruit juice rich in flavonoids.

2. The method according to claim 1, wherein in step (2), the separation is performed 1 time while separating all the fruit puree and all the fruit oil.

3. The method as claimed in claim 1, wherein in step (2), the separation is performed 2 times, primarily to separate the fruit puree and primarily to separate the fruit oil.

4. The method as claimed in claim 3, wherein, in the step (2), after the separation of the fruit paste and a part of the fruit oil and before the separation of the remaining fruit oil, the raw hippophae rhamnoides juice is left to stand at a temperature of 2-10 ℃ for more than 12 hours.

5. The method according to any one of claims 1 to 3, wherein in step (3), the number of solvent extractions is 2 or more, and the concentration of the solvent used in each time is the same or different.

6. A method according to any one of claims 1 to 3, wherein, in step (3), the concentrate has a relative density p of 1.00 to 1.30 g/ml.

7. The method according to any one of claims 1 to 3, wherein in step (4), the standing time is 1 to 14 hours.

8. A method according to any one of claims 1 to 3, wherein in step (4), the concentration is carried out until the relative density p of the liquid at 70-80 ℃ is 1.18-1.31 g/ml.

9. Use of the medicinal hippophae rhamnoides juice obtained by the method of any one of claims 1 to 8 for preparing a hippophae rhamnoides syrup.

10. The method of claim 9, wherein the medicinal hippophae rhamnoides juice is obtained entirely from hippophae rhamnoides fruit, and the concentration of the flavonoid component in the medicinal hippophae rhamnoides juice is 0.8-1.4 mg/ml.

Technical Field

The disclosure relates to the field of medicines, in particular to a production method of medicinal sea-buckthorn juice.

Background

Hippophae rhamnoides belongs to Elaeagnaceae, and is a plant with homology of medicine and food. The mature fruit of fructus Hippophae is fructus Hippophae. Seabuckthorn fruit is high in nutritive value, contains various vitamins, organic acids, amino acids, saccharides, trace elements and proteins, is particularly rich in vitamin C, is called as 'third fruit of human beings' and 'king of vitamin C', and is widely applied to the aspects of development and research of health care products, foods and the like.

The seabuckthorn fruit (the name of seabuckthorn dry fruit collected in Chinese pharmacopoeia) is a traditional medicinal material of Mongolia and Tibetan, has a long history as a traditional national medicinal material such as Mongolian and Tibetan medicines and has pharmacological effects of eliminating phlegm, benefiting lung, nourishing stomach, tonifying spleen, promoting blood circulation and removing blood stasis. The medicinal fructus Hippophae juice has effects of protecting liver, enhancing immunity, promoting growth and development, resisting and preventing cancer, resisting oxidation and resisting aging. The fructus Hippophae syrup has the advantages of No. approved for medicine, and has the main functions of relieving cough, eliminating phlegm, promoting digestion, removing food stagnation, dispelling blood stasis, and relieving cough, excessive phlegm, chronic bronchitis, chest distress, dyspepsia, and angina pectoris.

The prior sea-buckthorn fruit processing technology mainly aims at obtaining sea-buckthorn fruit juice beverage, and the obtained sea-buckthorn fruit juice beverage contains obviously low amount of medicinal effective components and can not meet the pharmaceutical requirements. The method for preparing the medicinal seabuckthorn fruit juice which contains enough medicinal effective components and has good stability by using seabuckthorn fruits as pharmaceutical raw materials has no better method at present.

At present, the method for preparing the raw juice of the sea buckthorn in domestic enterprises has the following three methods: (1) and (5) carrying out enzymolysis at normal temperature to prepare juice. Cleaning and crushing picked sea-buckthorn fruits, heating pulp to 65 ℃ by using a tubular heat exchanger, and maintaining for 15 minutes; then cooling to 45 ℃, adding pectin lyase, preserving heat, and carrying out enzymolysis for 2-3 hours; then cooling to normal temperature and squeezing to obtain juice. (2) And (5) heating to prepare juice. Cleaning fructus Hippophae, crushing, heating to 85 deg.C, and maintaining for 10-15 min; squeezing to obtain juice. (3) And (5) cooling to prepare juice. The fruit is frozen in a cold storage after being cleaned, and juice is extracted by direct squeezing or pulping after being unfrozen. In order to further obtain the sea-buckthorn clear juice, amylase, pectinase and pear juice enzyme are usually added to clarify the sea-buckthorn juice raw pulp, and the obtained clear juice is subjected to reduced pressure concentration to obtain the concentrated clear juice with the sugar degree of about 60%.

Generally, the current processing method cannot efficiently obtain medicinal effective components from the sea buckthorn berries; and in order to improve the stability of medicinal hippophae rhamnoides juice, stabilizers are generally added, which are not generally medicinal effective components and may have adverse effects on organisms after entering the medicine along with the hippophae rhamnoides juice.

Disclosure of Invention

The invention provides a production method of medicinal seabuckthorn fruit juice, which solves the problem that the medicinal value of seabuckthorn fruit juice prepared by the prior art is not high.

The production method of the medicinal seabuckthorn fruit juice provided by the disclosure comprises the following steps:

(1) squeezing fructus Hippophae, and separating fructus Hippophae juice and fruit residue;

(2) separating the raw juice of the sea buckthorn, and separating fruit paste, fruit oil and clear fruit juice;

(3) mixing the pomace separated in step (1) with the fruit oil and fruit paste separated in step (2) to form a pomace mixture; extracting effective components of flavonoids in the pomace mixture with a solvent, and filtering the extract; then removing the solvent in the filtrate to obtain a concentrated solution containing flavonoids;

(4) and (3) fully mixing the concentrated solution obtained in the step (3) and the clear juice obtained in the step (2) in sequence, standing, finely separating, and concentrating the separated clear liquid of the seabuckthorn juice to obtain the medicinal seabuckthorn juice rich in flavonoids.

In an embodiment of the disclosed method, in step (1), the sea buckthorn berries may be selected from frozen sea buckthorn berries or fresh sea buckthorn berries. The frozen sea buckthorn fruits are preferably squeezed, so that the finally obtained sea buckthorn juice not only contains high-content flavonoid active ingredients, but also has high vitamin C storage rate and is more beneficial to human health.

In an embodiment of the disclosed method, in step (1), the freezing temperature of the seabuckthorn fruits is-5 ℃ or less.

In an embodiment of the disclosed method, in step (1), the seabuckthorn fruit is pressed in the presence of purified water, for example in a mass ratio of seabuckthorn fruit to purified water of 2: 1.

In embodiments of the disclosed method, in step (2), the separation operation may be performed in any manner used in the art for achieving this purpose, for example, a centrifugal separation method may be employed.

In the embodiment of the disclosed method, in the step (2), the centrifugation may be performed 1 or more times, provided that the fruit paste and the fruit oil in the raw juice of hippophae rhamnoides can be sufficiently separated.

In one embodiment of the disclosed method, in step (2), the separation operation is performed 1 time, while separating out all the fruit puree and all the fruit oil.

In another embodiment of the disclosed process, in step (2), the separation is performed 2 times, first to separate mainly the puree and then mainly the fruit oil. The step of separating the fruit paste mainly means that the fruit paste formed by separating pulp substances is mainly separated for the first time, but part of fruit oil is additionally separated, because the raw juice of the sea buckthorn in the disclosure is suspension, part of the fruit oil can be wrapped in the fruit paste; the fruit oil is mainly separated in the second separation, but the residual fruit paste which is not completely separated in the first separation is additionally separated. Therefore, the fruit paste and the fruit pulp are sufficiently separated from the clear juice by performing the separation operation 2 times in steps, so that the stability of the target product in the subsequent pharmaceutical application is improved.

In a further embodiment of the disclosed method, in step (2), after separating the fruit paste and a portion of the fruit oil and before separating the remaining fruit oil, the raw hippophae rhamnoides juice is left to stand at a temperature of 2 ℃ to 10 ℃ for more than 12 hours to allow the fruit oil to be sufficiently layered in the raw hippophae rhamnoides juice. Standing at 2-10 deg.C to prevent vitamin C in fructus Hippophae natural juice from loss.

In a still further embodiment of the disclosed method, in step (2), the clear juice obtained after separation of the remaining fruit oil is stored at a temperature of 2 ℃ to 10 ℃ for later use. The vitamin C in the clear fruit juice can be kept or reduced as much as possible to be not lost when the clear fruit juice is stored at the temperature of 2-10 ℃.

In an embodiment of the disclosed method, in step (2), the 1-time simultaneous separation of all the puree and all the oil may be performed using any device capable of achieving this purpose in the art, such as a butterfly centrifuge (model: A213; manufacturer: Nanjing Nakawa oassay machines, Inc.) or a horizontal centrifuge (model: LW 355; manufacturer: Nanjing Nakawa oassay machines, Inc.); the separation of the fruit oil can be carried out using any device known in the art capable of achieving this purpose, for example, a disk centrifuge.

In an embodiment of the disclosed method, in step (3), the solvent may be selected from ethanol, so as to enable efficient extraction of flavonoid active ingredients from the pomace mixture without affecting the pharmaceutical performance of the flavonoid active ingredients and causing adverse effects on subsequent pharmacy, and to facilitate removal from the extract.

In a further embodiment of the disclosed method, in step (3), the volume concentration of ethanol used is 60 v% to 95 v%, such as 70 v% or 95 v%.

In a further embodiment of the disclosed method, in step (3), the mass of ethanol used is 1-4 times, such as 2-3 times, such as 2 times the mass of the pomace mixture, in order to sufficiently extract the flavonoid active ingredients in the pomace mixture.

In embodiments of the disclosed process, in step (3), the extraction operation may be, for example, reflux extraction, which is carried out in a manner of conventional operation used in the art for this purpose.

In a further embodiment of the disclosed method, in step (3), the number of solvent extractions is 2 or more, and the concentration of the solvent used each time is the same or different.

In still further embodiments of the disclosed methods, in step (3), the extraction with ethanol is performed 2 or more times. In the case of 2 extractions with ethanol, the concentration of ethanol used may be different, for example, a first time with higher concentration of ethanol and a second time with lower concentration of ethanol, thereby facilitating more thorough alcohol extraction. For example, ethanol is used in a concentration of 90 v% to 95 v% for the first time and 60 v% to 70 v% for the second time. In an embodiment of the disclosed method, the extraction time per time is between 1.5h and 3h, e.g. 1.5 h.

In an embodiment of the disclosed method, in step (3), the extracts of each time are combined, and then the solvent, such as ethanol, therein may be removed, for example, by concentration under reduced pressure, until the relative density ρ of the concentrated solution at 70-80 ℃ is 1.00-1.30g/ml, such as 1.00-1.10g/ml, and the solvent, such as ethanol, is considered to be substantially completely recovered and removed.

In embodiments of the disclosed method, in step (4), the mixing can be carried out using conventional equipment used in the art for this purpose, provided that the concentrate is well mixed with the clarified juice, e.g., using a blender.

In the method of the present disclosure, in step (3), the concentration of ethanol contained in the solvent extract, for example, the ethanol extract, is reduced by the concentration process, resulting in precipitation of a substance that is easily soluble in ethanol and hardly soluble in water. The concentration of the precipitate in the concentrate is high, and therefore, a dilution treatment is required to facilitate its separation. For example, water may be optionally added to achieve a dilution effect, then mixed with clear juice and concentrated, but this adds an additional process step.

In the method of the present disclosure, in the step (4), the concentrated solution obtained in the step (3) is directly mixed with the clear juice obtained in the step (2), so that the concentrated solution is diluted, the operation steps are reduced, energy is saved, and the process is better.

In the method disclosed by the invention, in the step (4), the concentrated solution containing the flavonoid substances extracted from the pomace mixture obtained in the step (3) is mixed with the clear fruit juice containing the flavonoid substances with the content not meeting the pharmaceutical requirements obtained in the step (2), so that the content of the flavonoid substances in the mixed solution is obviously improved, namely, the flavonoid substances serving as target substances are obtained from the seabuckthorn fruits as much as possible; the mixed liquid does not contain any substances that tend to cause instability of the mixed liquid, such as fruit oil and fruit puree, i.e., the mixed liquid has good stability.

In a further embodiment of step (4) of the disclosed method, the concentrated solution is mixed with the clear juice preserved under refrigeration, so that the mixed solution is rich in flavonoids, good in stability and high in vitamin C preservation rate.

In the embodiment of the disclosed method, in the step (4), after the concentrated solution and the clear juice are sufficiently mixed, the mixture is allowed to stand so that the precipitate generated by mixing the two is sufficiently precipitated. The standing temperature can be room temperature (18-26 ℃), and the standing time is 1-14 h.

In the embodiment of the disclosed method, in step (4), after standing, fine separation is performed, and the clear juice liquid is further separated, in other words, substances such as precipitation-like substances, foamy substances, and the like, which may cause instability of the mixed solution in subsequent uses (e.g., pharmaceutical uses) are further removed.

In an embodiment of the disclosed method, in step (4), the fine separation may be carried out in any manner, provided that the above-mentioned object is achieved. For example, mechanical separation, membrane separation or chemical separation, or a combination thereof, such as a combination of mechanical separation and membrane separation, or a combination of mechanical separation and chemical separation, may be employed, but is not limited thereto.

In an embodiment of the disclosed method, in step (4), the concentration may be performed in a manner of concentration under reduced pressure.

In the embodiment of the method of the present disclosure, in the step (4), the device used for concentration may be, for example, a concentration tank, but is not limited thereto.

In an embodiment of step (4) of the disclosed method, the concentration is performed until the relative density ρ of the liquid at 70-80 ℃ is 1.18-1.31g/ml, so as to obtain a quantitative medicinal hippophae rhamnoides juice, to improve the medicinal efficacy of the medicinal hippophae rhamnoides juice.

The disclosure also provides the use of the medicinal seabuckthorn juice obtained by the above method for preparing seabuckthorn syrup. In the application, the medicinal seabuckthorn fruit juice is completely obtained from seabuckthorn fruits, and the concentration of flavonoid components contained in the medicinal seabuckthorn fruit juice is 0.8-1.4 mg/ml.

The method for producing the medicinal seabuckthorn fruit juice can obtain the following effects:

1) the method disclosed by the invention takes the seabuckthorn fruits as raw materials, and designs specific operation steps, step sequences and the like, so that the full utilization of the seabuckthorn fruits is realized, medicinal effective components are fully obtained from the seabuckthorn raw juice and pomace, and the drug-loading rate of the finally prepared medicinal seabuckthorn fruit juice is remarkably increased.

2) The method disclosed by the invention is designed to realize the sufficient separation of the fruit oil, the fruit residues and the fruit paste by utilizing a specific separation mode and/or a specific combined separation mode in proper steps, so that the stability of the high drug-loading capacity sea-buckthorn juice is greatly improved.

3) In the implementation process of the method, non-medicinal components are not introduced into the medicinal seabuckthorn juice basically, so that the residue of the non-medicinal components in products prepared from the seabuckthorn juice, such as medicaments (such as seabuckthorn syrup), is reduced, and the side effect on organisms when the seabuckthorn juice prepared by the method is used for medical application can be further reduced.

4) The method also keeps the preservation rate of the vitamin C in the medicinal seabuckthorn fruit juice to the maximum extent, and further improves the comprehensive medicinal value of the medicinal seabuckthorn fruit juice.

Drawings

The accompanying drawings illustrate one exemplary embodiment of a method according to the present disclosure for further understanding of the disclosure. The accompanying drawings are incorporated in and constitute a part of this specification.

FIG. 1 is a flow diagram of one embodiment of a method of producing medicinal seabuckthorn juice according to the present disclosure.

Detailed Description

The present disclosure will be described in further detail with reference to the drawings and embodiments. It is to be understood that the specific embodiments described herein are for purposes of illustration only and are not to be construed as limitations of the present disclosure. It should be further noted that, for the convenience of description, only the portions relevant to the present disclosure are shown in the drawings. The present disclosure will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.