阅读说明：本技术 一种油井环保型微生物复合清防蜡剂 (Environment-friendly microbial composite paraffin removal and prevention agent for oil well ) 是由 王飞 何琴 于 2020-07-15 设计创作，主要内容包括：本发明提供一种油井环保型微生物复合清防蜡剂,包括嗜蜡混合菌液15～20份、菌种营养液15～60份、第一表面活性剂5～9份、第二表面活性剂5～9份、互溶剂2～4份；嗜蜡混合菌液为铜绿假单孢菌、枯草芽孢杆菌、土微球菌发酵液的混合液；菌种营养液的组分,包括红薯粉20～35份、液体石蜡1～5份、酒糟1～4份、琼脂1～3份、氯化钠1～2份、氯化铵1～2份、磷酸二氢钾2～7份、氯化钙1～2份、硫酸钠1～2份、水90～100份；第一表面活性剂为氟碳类表面活性剂FC-3B或油酸酰胺甜菜碱FDCH-1；第二表面活性剂为聚氧乙烯辛基苯酚醚-10或SP80；互溶剂为正辛醇或乙二醇单丁醚。该复合清防蜡剂有效解决了现有化学法和微生物法清防蜡的技术缺陷,具有经济环保、在稠油中溶蜡速度快、抗冲刷能力强、作用周期长等优点。(The invention provides an oil well environment-friendly microbial composite paraffin remover and inhibitor, which comprises 15-20 parts of a paraffin-philic mixed bacterial liquid, 15-60 parts of a strain nutrient solution, 5-9 parts of a first surfactant, 5-9 parts of a second surfactant and 2-4 parts of a mutual solvent; the wax-philic mixed bacterial liquid is a mixed liquid of pseudomonas aeruginosa, bacillus subtilis and micrococcus terreus fermentation liquid; the components of the strain nutrient solution comprise 20-35 parts of sweet potato powder, 1-5 parts of liquid paraffin, 1-4 parts of vinasse, 1-3 parts of agar, 1-2 parts of sodium chloride, 1-2 parts of ammonium chloride, 2-7 parts of monopotassium phosphate, 1-2 parts of calcium chloride, 1-2 parts of sodium sulfate and 90-100 parts of water; the first surfactant is a fluorocarbon surfactant FC-3B or oleamide betaine FDCH-1; the second surfactant is polyoxyethylene octyl phenol ether-10 or SP 80; the mutual solvent is n-octanol or ethylene glycol monobutyl ether. The composite wax removing and preventing agent effectively overcomes the technical defects of the existing chemical method and microbial method for removing and preventing wax, and has the advantages of economy, environmental protection, high wax dissolving speed in thick oil, strong anti-scouring capability, long acting period and the like.)

1. An oil well environment-friendly microbial composite paraffin remover and inhibitor is characterized by comprising 15-20 parts by mass of a paraffin-philic mixed bacterial liquid, 15-60 parts by mass of a strain nutrient solution, 5-9 parts by mass of a first surfactant, 5-9 parts by mass of a second surfactant and 2-4 parts by mass of an intersolunt;

the wax-philic mixed bacterial liquid is a mixed liquid of pseudomonas aeruginosa, bacillus subtilis and micrococcus terreus culture fermentation liquid in a volume ratio of 1:2: 1;

the strain nutrient solution comprises, by mass, 20-35 parts of sweet potato powder, 1-5 parts of liquid paraffin, 1-4 parts of vinasse, 1-3 parts of agar, 1-2 parts of sodium chloride, 1-2 parts of ammonium chloride, 2-7 parts of monopotassium phosphate, 1-2 parts of calcium chloride, 1-2 parts of sodium sulfate and 90-100 parts of water;

the first surfactant is one of a fluorocarbon surfactant FC-3B and oleamide betaine FDCH-1;

the second surfactant is one of polyoxyethylene octyl phenol ether-10 and SP 80;

the mutual solvent is one of n-octanol and ethylene glycol monobutyl ether.

2. The oil well environment-friendly type microbial composite paraffin remover and inhibitor as claimed in claim 1, wherein the amount of pseudomonas aeruginosa, bacillus subtilis and micrococcus terreus in the composite paraffin remover and inhibitor is 5-9 × 10⁸one/mL.

3. The use of the environmentally friendly microbial composite paraffin remover/inhibitor for oil wells according to claim 1 in oil field gathering and transportation operations.

Technical Field

The invention belongs to the technical field of oil field gathering and transportation, relates to the technical field of oil field gathering and transportation wax removal and prevention chemicals, and particularly relates to an environment-friendly microbial composite wax removal and prevention agent for an oil well. The invention solves the defects of high toxicity, non-ideal effect and the like of the existing paraffin removal and prevention agent through biological and chemical methods.

Background

Crude oil contains various amounts of paraffins, which are generally in solution under reservoir conditions. When crude oil rises from the bottom of a well and flows through an oil pipe, liquid paraffin in the crude oil is separated out along with the reduction of the temperature and the pressure of the pipeline and is adsorbed on the pipe wall of the oil pipe to cause wax deposition of the oil well, the harmfulness is high, the oil well can be directly blocked seriously, and the yield of the oil field is suddenly reduced or stopped. At present, the wax content of most of domestic oil fields is over 20 percent, for example, oil wells in the safety operation area of Changqing oil fields have the wax content of 25.46 percent, and the normal development and transportation of crude oil are influenced by the problems of wax precipitation and corrosion of the oil wells.

The most common paraffin removal method is to remove and prevent paraffin by a chemical method and dissolve paraffin by organic solvents such as benzene, xylene and the like, but the paraffin removal and prevention agents are flammable and explosive and have great harm to the environment and human bodies. The existing microbial paraffin remover and inhibitor in the market has the defects of weak anti-scouring capability, short action period, slow wax dissolving rate and the like.

Disclosure of Invention

Aiming at the technical defects of adopting a chemical method and a microbiological method to clear and prevent wax in the prior art, in particular to the use of a non-environment-friendly paraffin solvent, the invention provides an oil well environment-friendly microbial composite wax clearing and preventing agent. The invention achieves the purpose of removing and preventing wax by mixing the synergistic action of the wax-philic microorganism and the surfactant, and utilizing the metabolism of the microorganism in the stratum and the wetting and permeating action of the surfactant to quickly dissolve the solid paraffin on the pipe wall and the well rod and degrade the long-chain alkane in the crude oil. The composite wax removing and preventing agent is economical and environment-friendly, has high wax dissolving speed in thick oil, strong anti-scouring capability and long action period, and is an efficient and environment-friendly composite wax removing and preventing agent.

In order to achieve the above object, the present invention adopts the following technical solutions.

The environment-friendly microbial composite paraffin remover and inhibitor for the oil well comprises, by mass, 15-20 parts of a paraffin-philic mixed bacterial liquid, 15-60 parts of a strain nutrient solution, 5-9 parts of a first surfactant, 5-9 parts of a second surfactant and 2-4 parts of a mutual solvent.

The wax-philic mixed bacterial liquid is a mixed liquid of pseudomonas aeruginosa, bacillus subtilis and micrococcus terreus culture fermentation liquid in a volume ratio of 1:2: 1.

The strain nutrient solution comprises, by mass, 20-35 parts of sweet potato powder, 1-5 parts of liquid paraffin, 1-4 parts of vinasse, 1-3 parts of agar, 1-2 parts of sodium chloride, 1-2 parts of ammonium chloride, 2-7 parts of monopotassium phosphate, 1-2 parts of calcium chloride, 1-2 parts of sodium sulfate and 90-100 parts of water.

The first surfactant is one of fluorocarbon surfactant FC-3B and oleamide betaine FDCH-1.

The second surfactant is one of polyoxyethylene octyl phenol ether-10 and SP 80.

The mutual solvent is one of n-octanol and ethylene glycol monobutyl ether.

The paraffin dissolution rate of a single wax-philic strain is often limited because the mixed strain is divided into different fractions for processing crude oil components such as wax, colloid and asphaltene in the crude oil. In addition, the oil well operation environment is different from the crude oil stratum environment, and the tolerance and the suitability of a single wax-philic strain to different environments are also different. In order to improve the dissolving rate of the wax-philic strains to paraffin and improve the universality of microbial wax-removing and preventing products, the invention screens and compounds various wax-philic strains for use, and determines the wax-philic mixed bacterial liquid used by combining the wax-philic strains of Pseudomonas, Bacillus and Micrococcus after a plurality of attempts. Specifically, the Pseudomonas aeruginosa is selected from the cerophilic wax strains of the Pseudomonas aeruginosa, and is preserved in China general microbiological culture collection center in 2009 for 1 month and 19 days, wherein the preservation number is CGMCC 1.9054; bacillus subtilis (Bacillus subtilis) is selected from Bacillus cereus strain, and is preserved in China general microbiological culture collection center in 1972 in 3 months and 1 day, with the preservation number of CGMCC 1.821; micrococcus cerophilus is selected from Micrococcus terreus (Micrococcus terreus), and is preserved in China general microbiological culture Collection center in 2007 in 1 month and 12 days, with the preservation number of CGMCC 1.7054. Pseudomonas aeruginosa, bacillus subtilis and micrococcus terreus strains used in the invention are all purchased from China general microbiological culture collection center. The culture fermentation broth is preferably an expanded culture fermentation broth in a logarithmic growth phase, the compound volume ratio of the pseudomonas aeruginosa culture fermentation broth to the pseudomonas subtilis culture broth to the micrococcus terrae culture fermentation broth is 1:2:1, and the culture broth has a better characteristic on the degradation effect of paraffin.

The strain nutrient solution is a specially-prepared mixed liquid which is suitable for coexistence of pseudomonas aeruginosa, bacillus subtilis and micrococcus terreus and keeps excellent microbial performance. The first surfactant and the second surfactant selected by the invention are products developed and produced by Shaanxi Fengden petrochemical company Limited, and have good compatibility with wax-philic mixed bacteria liquid and strain nutrient solution. The mutual solvent is one of n-octanol and ethylene glycol monobutyl ether, and is a commercially available product.

Through determination, the amount of the pseudomonas aeruginosa, the bacillus subtilis and the micrococcus terreus in the composite paraffin remover and inhibitor is 5-9 × 10⁸one/mL.

In addition, the invention also provides application of the oil well environment-friendly microbial composite paraffin removal and prevention agent in oil field gathering and transportation operation. The composite wax cleaning and preventing agent has stronger adsorption force and anti-scouring capability, microorganisms adsorbed on the wall of an oil pipe cannot be obviously reduced due to the scouring effect, so that the action period of the wax cleaning and preventing agent is prolonged, and the wax dissolving rate reaches 0.044 g/min-0.049 g/min.

The field application proves that the composite paraffin removal and prevention agent of the invention has SO4 on the stratum^2-The ions have certain consumption effect, can reduce substrates required by sulfate reducing bacteria, reduce the activity of the sulfate reducing bacteria, not only can play a role in wax prevention and wax removal, but also can inhibit the growth and reproduction of the sulfate reducing bacteria and play a role in inhibiting corrosion of metal pipelines.

Compared with the prior art, the oil well environment-friendly microbial composite paraffin removal and prevention agent disclosed by the invention at least has the following beneficial effects or advantages.

1) The oil well environment-friendly microbial composite paraffin remover and inhibitor comprises, by mass, 15-20 parts of a paraffin-philic mixed bacterial liquid, 15-60 parts of a strain nutrient solution, 5-9 parts of a first surfactant, 5-9 parts of a second surfactant and 2-4 parts of a mutual solvent. Through multiple test trials of the inventor of the invention, the paraffin-philic mixed bacterial liquid is a mixed liquid of pseudomonas aeruginosa, bacillus subtilis and micrococcus terreus culture fermentation liquid in a volume ratio of 1:2:1, and has an excellent degradation effect on paraffin in crude oil.

2) The oil well environment-friendly microbial composite paraffin removal and prevention agent disclosed by the invention is environment-friendly, and has a high paraffin dissolution rate, and the bacterial concentration reaches 5-9 × 10⁸The wax cleaning agent has the advantages that the wax cleaning agent has a high adsorption capacity and a high anti-scouring capacity, microorganisms adsorbed on the wall of an oil pipe cannot be obviously reduced due to the scouring effect, the action period of the wax cleaning agent is prolonged, and the wax dissolving rate reaches 0.044-0.049 g/min.

3) The oil well environment-friendly microbial composite paraffin removal and prevention agent can consume SO4 in stratum^2-The ions not only can play a role in wax prevention and wax removal, but also play a role in corrosion inhibition on the metal pipeline.

Detailed Description

In order to facilitate understanding of the objects, technical solutions and effects of the present invention, the present invention will be further described in detail with reference to examples.