阅读说明：本技术 一种基于数字照片批处理的微小液滴群流式尺寸统计方法 (Micro-droplet cluster flow type size statistical method based on digital photo batch processing ) 是由 张乐翔 于 2019-03-14 设计创作，主要内容包括：本发明公开了一种基于数字照片批处理的微小液滴群流式尺寸统计方法,包括流体通道,所述流体通道的形状为倒“T”形结构。本发明一种基于数字照片批处理的微小液滴群流式尺寸统计方法,通过为该统计方法配套设置有流体通道、注液泵及快速摄像机,注液泵是配套连接在流体通道上端且对流体通道内注入待测液滴的,且流体通道两侧下端配套设置有下游区域,下游区域的正前方在空间上配套设置有快速摄像机,对流过下游区域完成快速拍照工作,本发明的目的在于建立一套基于快速摄像机流式拍照的快速、精准、高通量,且能自动统计分析的微小液滴群尺寸、频率等水力学参数的统计方法,可见该发明,功能实用,适合广泛推广。(The invention discloses a micro-droplet cluster flow type size statistical method based on digital photo batch processing. The invention relates to a small liquid drop group flow type size statistical method based on digital photo batch processing, which is characterized in that a fluid channel, a liquid injection pump and a quick camera are matched and arranged for the statistical method, the liquid injection pump is matched and connected to the upper end of the fluid channel and injects liquid drops to be detected into the fluid channel, the lower ends of the two sides of the fluid channel are matched and provided with downstream areas, the quick camera is matched and arranged in space right in front of the downstream areas, and quick photographing work is completed on the downstream areas.)

1. A flow-type size statistic device for micro-droplet group based on digital photo batch processing, comprising a fluid channel (1), characterized in that: the shape of fluid passage (1) is for falling "T" shape structure, the width of fluid passage (1) is 0.5cm, the both ends of fluid passage (1) are crossed mouthful turn-on connection and are had low reaches region (4), the upper end matching nature of fluid passage (1) is connected with the liquid pump, the inside of fluid passage (1) is leading-in through the liquid pump has liquid droplet (2), the cross section of liquid droplet (2) is L-L cross-section (3), two the dead ahead of low reaches region (4) matches in the space and is provided with quick camera.

2. The apparatus of claim 1, wherein the apparatus comprises: the fluid channel (1) and the downstream area (4) are of an integrated conducting structure, and the fluid channel (1) and the downstream area (4) are of transparent structures.

3. The method for counting the flow size of the micro-droplet group based on the digital photo batch processing according to claim 1 comprises the following steps:

the first step is as follows: and opening the liquid injection pump, introducing the micro-droplets (2) into the fluid channel (1), dispersing the micro-droplets one by the external phase, and then sequentially flowing through the photographed downstream area (4).

The second step is that: the photographs were taken with a fast camera focus, and several thousand photographs contained dynamic information about the flow of the population of droplets (2).

The third step: and (3) importing the MATLAB by using an imread function picture by picture, identifying the channel structure of the downstream region (4), and intercepting the channel region to be analyzed.

The fourth step: background color removal, where the background color may be averaged over hundreds of photos, or taking photos when only the external phase was originally on, in order to remove ambient light and other interference.

The fifth step: then, the gray picture is converted, and the gas phase and the liquid phase are digitally distinguished by gray values: "1" represents the droplet (2) and "0" represents the outer phase.

And a sixth step: the length of each liquid column on each picture is obtained by counting pixel points and comparing the pixel points with the width of a known channel in the picture, and the frequency of the liquid drops (2) can be obtained by the serial number of the pictures because the pictures are taken intermittently at equal time.

The seventh step: in order to sequence gas and liquid in an actual time sequence, an L-L section (3) which is 30 pixels away from the downstream of the intersection of the T-shaped fluid channel (1) is selected for judgment, and the specific situation is shown in figure three.

Eighth step: when the L-L section (3) is occupied by a drop (2) in the ith photograph and by an external phase in the (i + 1) th photograph, a new drop (2) is formed, recording its length and frequency; when the section L-L (3) of the i-th and i + 1-th photographs is the droplet (2), it indicates that a droplet (2) is being formed, and no recording is performed at this time.

4. The method for counting the flow size of the micro liquid drop clusters based on the digital photo batch processing as claimed in claim 1, wherein the lengths of the liquid drops and the oil columns are specifically calculated as follows:

the width w of the fluid channel (1) is 0.5cm and consists of p0 pixels, when the liquid drop (2) and the external phase between the liquid drop are drawn into line segments, MATLAB reads how many pixels each line segment consists of, for example pi, the length of the line segment ni is equal to wpi/p0, and i is 1, 2, 3 and 4. . . (wherein, the line segment with the numerical value of "0" is abstracted by the oil column, and the line segment with the numerical value of "1" is abstracted by the liquid drop).

5. The method for counting the flow size of micro liquid drop clusters based on digital photo batch processing as claimed in claim 1, wherein the frequency of sequentially recording and calculating the liquid drops and oil columns is specifically calculated as follows:

from the above, the lengths of the droplet (2) and the oil column are obtained for each frame of picture, but the same droplet (2) appears in several frames of pictures, and information on the droplets (2) flowing through is arranged and recorded in sequence.

The section (3) of the upstream fixed position L-L is selected for judgment, and four conditions are summarized:

when the i and i +1 frames of pictures are taken, the L-L section (3) is '1', namely a liquid drop (2), and represents that one liquid drop (2) passes through without recording;

(II) when the i-th and i + 1-th frames of pictures are taken, the L-L section (3) is 0, namely an outer phase, which represents that an outer phase slug passes through without recording;

(III) when the L-L section (3) is sequentially '1' and '0' on the i-th and i + 1-th pictures, which represent that a liquid drop (2) just passes through is recorded, the pictures shot by the rapid camera are shot at equal time intervals, for example 2000 pictures are shot in one second, the liquid drop (2) flows through the L-L section (3) for n frames, the frequency of the liquid drop (2) is n/2000Hz, and the time of each liquid drop (2) passing through the L-L section (3) is known;

and (IV) when the L-L section (3) is sequentially '0' and '1' on the i-th and i + 1-th pictures, the picture just passes through one outer phase slug, and the information of the outer phase slug is recorded.

By this rule, a sequential arrangement is achieved, obtaining the frequency parameters of the droplets (2).

Technical Field

The invention relates to a micro-droplet cluster flow type size statistical method based on digital photo batch processing, in particular to a flow type size statistical method for micro-droplet clusters, and belongs to the technical field of statistics.

Background

Microfluidic technologies based on micro-droplet populations have been applied to a variety of fields such as high-throughput drug screening, efficient and controllable synthesis of functional materials, molecular biology based on single molecules and single cell levels, cell biology and synthetic biology research, clinical examination, proteome research, and the like.

The detection of the size of the micro liquid drop mainly depends on photographic analysis, or photographic statistics after the liquid drop is statically placed and distributed, but the analysis is difficult when the liquid drop is distributed in multiple layers; or taking a picture recording under the streaming condition, and then making statistics from hundreds of photos, the process is complicated.

Disclosure of Invention

The invention aims to overcome the defects in the prior art and provides a micro liquid drop group flow type size statistical method based on digital photo batch processing, which is simple in structure and reasonable in design.

In order to achieve the purpose, the invention adopts the technical scheme that:

according to the technical scheme provided by the invention: the utility model provides a little liquid drop crowd STREAMING size statistics device based on digital photo is batch processing, includes fluid passage, fluid passage's shape is for "T" shape structure, fluid passage's width is 0.5cm, fluid passage's both ends mouth turn-on connection that intersects has the low reaches region, fluid passage's upper end matching nature is connected with the liquid pump, fluid passage's inside is leading-in to have the liquid drop through the liquid pump, the cross section of liquid drop is L-L cross-section, two the regional dead ahead of low reaches matches in the space and is provided with the quick camera.

As a further improvement of the invention, an integral conducting structure is arranged between the fluid channel and the downstream area, and the fluid channel and the downstream area are both transparent structures.

As a further improvement of the invention, the operation steps of the micro-droplet flow-type size statistical method based on the digital photo batch processing are as follows:

the first step is as follows: and opening the liquid injection pump, introducing the micro-droplets into the fluid channel, dispersing the micro-droplets by the external phase one by one, and then sequentially flowing through the photographed downstream area.

The second step is that: pictures were taken with a fast camera focusing, thousands of pictures containing dynamic information of the flow of the population of droplets.

The third step: and (3) importing the pictures into MATLAB one by using an imread function, identifying the channel structure of the downstream region, and intercepting the channel region to be analyzed.

The fourth step: background color removal, where the background color may be averaged over hundreds of photos, or taking photos when only the external phase was originally on, in order to remove ambient light and other interference.

The fifth step: then, the gray picture is converted, and the gas phase and the liquid phase are digitally distinguished by gray values: "1" represents a droplet and "0" represents the outer phase.

And a sixth step: the length of each liquid column on each picture is obtained by counting pixel points and comparing the pixel points with the width of a known channel in the picture, and the frequency of liquid drops can be obtained by the serial number of the picture, because the pictures are taken at equal time intervals.

The seventh step: in order to sequence gas and liquid in an actual time sequence, an L-L section which is 30 pixels away from the downstream of the T-shaped fluid channel junction is selected for judgment, and the specific situation is shown in the attached diagram III.

Eighth step: when the L-L section is occupied by a droplet in the ith photograph and by an outer phase in the (i + 1) th photograph, a droplet is newly formed, and its length and frequency are recorded; when the section L-L of the i-th and i + 1-th photo is a droplet, it indicates that a droplet is being formed, and no recording is performed at this time.

As a further improvement of the invention, the method for counting the flow size of the micro-droplet group based on digital photo batch processing specifically calculates the lengths of the droplets and the oil column as follows:

the width w of the fluid channel is 0.5cm and is composed of p0 pixels, when the droplets and the outer phase between the droplets are drawn into line segments, MATLAB reads how many pixels each line segment is composed of, for example pi, the length of the line segment ni is equal to wpi/p0, and i is 1, 2, 3 and 4. . . .

As a further improvement of the invention, the method for counting the flow size of the micro-droplet group based on digital photo batch processing comprises the following specific calculation steps of sequentially recording and calculating the frequency of the droplets and the oil column:

from the above, the lengths of the droplets and the oil column on each frame of the picture are obtained, but the same droplets will appear in several frames of the picture, and information on the droplets flowing through is arranged and recorded in sequence.

And (3) selecting an upstream fixed position L-L section for judgment, and summarizing four conditions:

when the i-th and i + 1-th frames of pictures are taken, the L-L section is '1', namely a liquid drop, which represents that a liquid drop passes through and is not recorded;

when the i and i +1 frames of pictures are photographed, the L-L section is 0, namely the outer phase, which represents that an outer phase slug passes through and is not recorded;

when the L-L section is sequentially 1 and 0 on the i-th and i + 1-th pictures, which represent that a liquid drop just passes through, the information of the liquid drop is recorded, the pictures shot by the quick camera are taken at equal time intervals, for example 2000 pictures are taken within one second, the liquid drop flows through the L-L section for a total of n frames, the frequency of the liquid drop is n/2000Hz, and the time of each liquid drop passing through the L-L section is known;

when the L-L section is "0" and "1" in the i and i +1 th pictures in turn, it represents that just passing through an outer phase slug, and records the information of the outer phase slug.

By this rule, a sequential arrangement is achieved, obtaining the frequency parameters of the droplets.

Compared with the prior art, the invention has the following advantages:

1) the invention relates to a micro liquid drop group flow type size statistical method based on digital photo batch processing, wherein a fluid channel, a liquid injection pump and a quick camera are matched with the statistical method, the liquid injection pump is matched and connected to the upper end of the fluid channel and injects liquid drops to be detected into the fluid channel, downstream areas are matched and arranged at the lower ends of the two sides of the fluid channel, the quick camera is matched and arranged on the space right in front of the downstream areas, and quick photographing work is completed on the liquid drops flowing through the downstream areas.

2) The invention relates to a micro-droplet cluster flow type size statistical method based on digital photo batch processing, which is characterized in that an Imread function is used for introducing MATLAB into photos taken by a quick camera one by one, a channel structure is identified, a channel region is intercepted and is to be analyzed, the micro-droplet cluster flow type size statistical method is mainly used for processing a large number of photo sequences stored by the quick camera in batch by means of MATLAB programming software, and aims to establish a set of statistical method for quickly, accurately and high-flux based quick and high-flux flow type photographing of the quick camera and capable of automatically counting and analyzing hydraulic parameters such as size, frequency and the like of micro-droplet clusters, and through the invention, the length and frequency information of some liquid droplets and oil columns in a period can be automatically counted from thousands of photos and can be stored in order, so that an analysis scheme for quickly and automatically counting the sizes of the micro-droplet clusters in batch is realized, therefore, the invention has practical, is suitable for being widely popularized.

Drawings

FIG. 1 is a schematic structural diagram of the steps of the flow measurement statistical method for the size of micro-droplet clusters.

FIG. 2 is a schematic diagram of the structure of the process for processing and obtaining the length of the gas-liquid column in a single photo.

FIG. 3 is a schematic diagram of the structure of the index recorded by the liquid drop and external phase sequencing of the present invention.

In the figure: 1. a fluid channel; 2. a droplet; 3. L-L cross section; 4. a downstream region.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.

As shown in fig. 1-3, a small droplet group flow type size statistical apparatus based on digital photo batch processing includes a fluid channel 1, the fluid channel 1 is in an inverted T-shaped structure, the width of the fluid channel 1 is 0.5cm, two end intersection ports of the fluid channel 1 are connected with downstream regions 4 in a conduction manner, the upper end of the fluid channel 1 is connected with a liquid injection pump in a matching manner, droplets 2 are introduced into the fluid channel 1 through the liquid injection pump, the cross section of the droplets 2 is an L-L cross section 3, a fast camera is spatially matched and arranged right in front of the two downstream regions 4, the statistical method is provided with the fluid channel 1, the liquid injection pump and the fast camera in a matching manner, the liquid injection pump is connected to the upper end of the fluid channel 1 and injects the droplets 2 to be measured into the fluid channel 1, and the lower ends of two sides of the fluid channel 1 are provided with the downstream regions 4 in a matching manner, a fast camera is arranged right in front of the downstream area 4 in a matched manner in space, and fast photographing is completed on the liquid drops 2 flowing through the downstream area 4.

As shown in fig. 2, the fluid channel 1 and the downstream area 4 are of an integral conduction structure, the fluid channel 1 and the downstream area 4 are both of transparent structures, conduction between the fluid channel 1 and the downstream area 4 enables the liquid droplet 2 to be measured injected into the fluid channel 1 to smoothly flow into the downstream area 4, and the fluid channel 1 and the downstream area 4 are of transparent structures, so that a clear state picture of the liquid droplet 2 flowing through the fluid channel 1 and the downstream area 4 can be conveniently taken by a fast camera.

Referring to fig. 1-3, a method for counting the flow size of a micro-droplet group based on digital photo batch processing comprises the following steps:

the first step is as follows: the liquid injection pump is turned on, the micro-droplets 2 are guided into the fluid channel 1, and after being dispersed one by the external phase, the micro-droplets sequentially flow through the photographed downstream area 4.

The second step is that: pictures were taken with a fast camera focus, several thousand pictures containing dynamic information of the flow of the population of droplets 2.

The third step: and (3) importing the images into MATLAB one by using an imread function, identifying the channel structure of the downstream region 4, and intercepting the channel region to be analyzed.

The fourth step: background color removal, where the background color may be averaged over hundreds of photos, or taking photos when only the external phase was originally on, in order to remove ambient light and other interference.

The fifth step: then, the gray picture is converted, and the gas phase and the liquid phase are digitally distinguished by gray values: "1" represents droplet 2 and "0" represents the outer phase.

And a sixth step: the length of each liquid column on each picture is obtained by counting pixel points and comparing the pixel points with the width of a known channel in the picture, and the frequency of the liquid drops 2 can be obtained by the serial number of the picture, because the picture is obtained by taking pictures at equal time intervals.

The seventh step: in order to sequence gas and liquid in an actual time sequence, an L-L section 3 which is 30 pixels away from the downstream of the intersection of the T-shaped fluid channel 1 is selected for judgment, and the specific situation is shown in figure three.

Eighth step: when the L-L section 3 is occupied by the drop 2 in the ith picture and by the outer phase in the (i + 1) th picture, a new drop 2 is formed, its length and frequency being recorded; when the section 3L-L is the droplet 2 in the i-th and i + 1-th photographs, it indicates that a droplet 2 is being formed, and no recording is performed at this time.

Referring to fig. 2-3, in a method for counting the flow size of a micro-droplet group based on digital photo batch processing, the lengths of the droplets and the oil column are specifically calculated as follows:

first, the width w of the fluid channel 1 is 0.5cm, and is composed of p0 pixels, when the droplet 2 and the outer phase therebetween are drawn into line segments, the MATLAB reads how many pixels each line segment is composed of, for example, pi, the length of the line segment ni is equal to wpi/p0, i is 1, 2, 3, 4. . . The line segment with the numerical value of "0" is abstracted by the oil column, and the line segment with the numerical value of "1" is abstracted by the liquid drop.

Referring to fig. 2-3, a flow-type size statistical method of micro-droplets based on digital photo batch processing, which records and calculates the frequency of droplets and oil columns in sequence as follows:

from the above, the lengths of the droplet 2 and the oil column are obtained for each frame of the picture, but the same droplet 2 appears in several frames of the picture, and information on the droplets 2 flowing through is arranged and recorded in sequence.

The upstream fixed position L-L section 3 was selected for determination, and four cases were summarized:

firstly, when the i-th and i + 1-th frames of pictures are taken, the L-L section 3 is '1', namely a liquid drop 2, which represents that one liquid drop 2 passes through and is not recorded;

secondly, when the i and i +1 frames of pictures are photographed, the L-L section 3 is '0', namely an external phase, which represents that an external phase slug passes through without recording;

thirdly, when the L-L section 3 is sequentially '1' and '0' on the i-th and i + 1-th frame pictures, which represent that a liquid drop 2 just passes through, the information of the liquid drop 2 is recorded, the pictures shot by the fast camera are taken intermittently at equal time, for example, 2000 pictures are taken within one second, the liquid drop 2 flows through the L-L section 3 for n frames, the frequency of the liquid drop 2 is n/2000Hz, and the time of each liquid drop 2 passing through the L-L section 3 is known;

and fourthly, when the L-L section 3 is sequentially '0' and '1' on the i-th and i + 1-th pictures, the picture represents that an external phase slug just passes through, and the information of the external phase slug is recorded.

By this rule, a sequential arrangement is achieved, obtaining the frequency parameters of the droplets 2.

The invention relates to a micro-droplet flow type size statistical method based on digital photo batch processing, which comprises the following specific calculation methods:

1. droplet and oil column length:

knowing that the width w of the flow channel 1 in fig. 2b is 0.5cm and consists of p0 pixels, when converting each picture into fig. 2d, MATLAB can read how many pixels each line on the graph consists of, e.g., pi, and the length of the line ni is equal to wpi/p0, i is 1, 2, 3, 4. . .

The line segment with the numerical value of "0" is abstracted by the oil column, and the line segment with the numerical value of "1" is abstracted by the liquid drop.

2. The frequency of the droplets 2 and the oil column was recorded and calculated in order:

from the above, we have obtained the length of the droplet 2 and the oil column on each frame of picture, but the same droplet 2 will appear in several frames of pictures, and the researcher only wants to order and record the information of the droplets 2 flowing through.

We selected upstream fixed position L-L section 3 for the determination, and four cases are summarized in table 1:

(I) when the i and i +1 th frames of pictures are taken, the L-L section 3 is '1', namely a liquid drop 2, which represents that one liquid drop 2 passes through, and no record is made;

(II) when the i and i +1 frames of pictures are taken, the L-L section 3 is '0', namely an external phase, which represents that an external phase slug is passing through, and no record is made;

(III) when the L-L section 3 is sequentially '1' and '0' on the i-th and i + 1-th pictures, which represent that a liquid drop 2 just passes through, we record the information of the liquid drop 2, because the pictures taken by the fast camera are taken intermittently at equal time, for example 2000 pictures taken in one second, the liquid drop 2 flows through the L-L section 3 for n frames, the frequency of the liquid drop 2 is n/2000Hz, and the time of each liquid drop 2 passing through the L-L section 3 is known;

(IV) when the L-L section 3 is "0" and "1" in the i-th and i + 1-th pictures in turn, it represents that just passing through an outer phase slug, we record the information of this outer phase slug.

By this rule we achieve a sequential arrangement and obtain the frequency parameters of the droplets 2.

In summary, according to the invention, the length and frequency information of the liquid drop 2 and the oil column which are frequently counted from thousands of photos can be automatically stored.

The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.