阅读说明：本技术 核酸提取用提取盒的活塞 (Piston of extraction box for nucleic acid extraction ) 是由 赵荣植 李孝根 朴海准 李善永 林冠勋 金芢爱 金宰永 朴婋林 金彤宪 于 2018-12-20 设计创作，主要内容包括：本发明提供一种核酸提取用提取盒的活塞,本发明包括：上部主体,呈圆筒状,具有中空；下部主体,与上述上部主体的一端相结合,具有以按规定角度隔开的方式配置的两个接口；控制棒模块,通过与上述上部主体的另一端相结合来密封上述另一端,沿着上述中空上下移动；以及旋转调节模块,通过与上述下部主体的轴相结合来向上述下部主体传递驱动力。(The present invention provides a piston of an extraction cartridge for nucleic acid extraction, comprising: an upper body having a hollow cylindrical shape; a lower body coupled to one end of the upper body and having two ports arranged to be spaced apart from each other at a predetermined angle; a control rod module which is combined with the other end of the upper main body to seal the other end and moves up and down along the hollow space; and a rotation adjusting module which transmits a driving force to the lower body by being coupled to a shaft of the lower body.)

1. A plunger of an extraction cassette for nucleic acid extraction, which extracts nucleic acid by sequentially sucking and mixing a sample and a plurality of reagents arranged in the extraction cassette, comprising:

an upper body having a hollow cylindrical shape;

a lower body coupled to one end of the upper body and having two ports arranged to be spaced apart from each other at a predetermined angle;

a control rod module which is combined with the other end of the upper main body to seal the other end and moves up and down along the hollow space; and

and a rotation adjusting module coupled to the shaft of the lower body to transmit a driving force to the lower body.

2. The plunger of the nucleic acid isolation cassette according to claim 1, wherein the lower body includes:

the body is disc-shaped;

a shaft formed to protrude outward from the center of the body; and

the liquid port and the filter port are arranged to be spaced apart from the center by the same distance.

3. The plunger of an extraction cassette for nucleic acid isolation according to claim 2, wherein the filter interface comprises:

a glass fiber filter having a plurality of particle sizes; and

and a support structure for fixing the filter.

4. The plunger of the cartridge for nucleic acid isolation according to claim 3, wherein the support structure is made of a porous plastic material having a predetermined particle size so that the filter is prevented from being detached and a predetermined pressure is maintained when the liquid is discharged.

5. The plunger of the nucleic acid isolation cassette according to claim 2, wherein a coupling structure that engages with the main body of the lower body is formed at one end of the upper body, and the coupling structure has a first hole connected to the liquid port and a second hole connected to the filter port.

6. The piston of the nucleic acid isolation cassette according to claim 5, wherein the second hole is formed so as to have a diameter smaller than that of the filter port, so that the support structure and the filter can be prevented from coming off.

7. The piston of the nucleic acid isolation cassette according to claim 2, wherein a groove recessed toward the center is formed in an outer periphery of the main body.

8. The piston of the nucleic acid extraction cartridge according to claim 2, wherein the rotation regulating means comprises:

a coupling groove formed at a center portion of one surface of the rotation adjusting module in such a manner as to be engaged with the shaft; and

and a driving groove formed on the other surface of the rotation regulating module so as to engage with a driving shaft of the nucleic acid isolation apparatus.

9. The piston of the nucleic acid extraction cartridge according to claim 1, wherein the control rod module comprises:

a coupling part coupled to a control rod of the nucleic acid extracting apparatus; and

and a contact portion surrounding an outer periphery of the coupling portion and moving to contact an inner wall of the upper body.

10. The plunger of the nucleic acid isolation cassette according to claim 1, wherein the two ports are disposed at an angle of 18 ° to 36 ° apart from each other.

Technical Field

The present invention relates to a plunger of an extraction cassette for nucleic acid extraction, and more particularly, to a plunger suitable for an extraction cassette that can separate and purify nucleic acid from a sample for the purpose of amplifying the nucleic acid.

Background

With the development of modern life engineering techniques, the causes of diseases can be explained at the genetic level. Accordingly, there is an increasing demand for the manipulation and biochemical analysis of biological samples for the cure or prevention of human diseases.

Meanwhile, in addition to diagnosis of diseases, a technique for extracting and analyzing nucleic acids from a sample containing biological samples or cells is required in various fields of new drug development, preliminary examination of whether or not viruses or bacteria are infected, forensic medicine, and the like.

In the conventional nucleic acid extracting apparatus, a separate apparatus is required for each pretreatment step (concentration and purification), and it takes a long time to move to another apparatus after one pretreatment step is finished.

In the united states issued patent US 6374684 (first patent document), a plurality of branched flow paths 34, 38, 42, 44 are provided in the piston head, and mixing can be performed in the piston interior space by rotating the piston head and directly sucking in the reagent present in the chamber. However, the extraction cartridge disclosed in the first patent document has a problem that the production and use efficiency of the device can be reduced because the structure of the piston is complicated.

Therefore, in order to solve such problems, a piston of an extraction cassette for nucleic acid extraction having a simpler structure is being studied.

Disclosure of Invention

(problem of the technology)

The purpose of the present invention is to provide a plunger for an extraction cassette for nucleic acid extraction, which has a simpler structure and is effective.

(means for solving the problems)

In order to achieve the above object, a plunger of an extraction cassette for nucleic acid extraction according to an embodiment of the present invention includes: an upper body having a hollow cylindrical shape; a lower body coupled to one end of the upper body and having two ports arranged to be spaced apart from each other at a predetermined angle; a control rod module which is combined with the other end of the upper main body to seal the other end and moves up and down along the hollow space; and a rotation adjusting module which transmits a driving force to the lower body by being coupled to a shaft of the lower body.

According to an embodiment of the present invention, the lower body includes: the body is disc-shaped; a shaft formed to protrude outward from the center of the body; and a liquid port and a filter port which are arranged to be spaced apart from the center by the same distance.

According to an embodiment of the present invention, the filter interface includes: a glass fiber filter having a plurality of particle sizes; and a support structure for fixing the filter.

According to an embodiment of the present invention, the support structure is made of a porous plastic material having a predetermined particle size, so that the filter is prevented from being detached and a predetermined pressure is maintained when the liquid is discharged.

According to an embodiment of the present invention, a coupling structure engaged with the body of the lower body is formed at one end of the upper body, and the coupling structure has a first hole connected to the liquid port and a second hole connected to the filter port.

According to an embodiment of the present invention, the second hole is formed to have a diameter smaller than that of the filter interface so as to prevent the support structure and the filter from being detached from each other.

According to an embodiment of the present invention, a groove recessed toward the center direction is formed on an outer periphery of the body.

According to an embodiment of the present invention, the rotation adjusting module includes: a coupling groove formed at a center portion of one surface of the rotation adjusting module in such a manner as to be engaged with the shaft; and a driving groove formed on the other surface of the rotation regulating module so as to engage with a driving shaft of the nucleic acid extracting apparatus.

According to an embodiment of the present invention, the control rod module includes: a coupling part coupled to a control rod of the nucleic acid extracting apparatus; and a close contact portion surrounding an outer periphery of the coupling portion and moving to be closely contacted to an inner wall of the upper body.

According to an embodiment of the present invention, the two interfaces are configured to be spaced apart from each other by 18 ° to 36 °.

(effect of the invention)

According to the plunger of the nucleic acid extraction cartridge of an embodiment of the present invention, the upper body for mixing the sample and the reagent and the lower body for sucking and discharging the reagent are separated from each other, thereby simplifying the structure of the plunger. The manufacturing and assembling processes of the piston can be effectively performed.

Further, the reagent suction step and the reagent discharge step can be sequentially performed by the rotation of the piston by providing the liquid port and the filter port disposed at a predetermined angle from each other in the piston lower main body. This can improve the efficiency of the nucleic acid extraction step.

Drawings

FIG. 1 is a bottom perspective view of a plunger of an extraction cassette for nucleic acid extraction according to one embodiment of the present invention;

FIG. 2 is a top view of the lower piston body of one embodiment of the present invention;

FIG. 3 is a perspective view of an extraction cassette for a nucleic acid extraction apparatus according to one embodiment of the present invention;

FIG. 4 is a bottom perspective view of an extraction cartridge for use with the present invention;

fig. 5 is an exploded view of the extraction cartridge shown in fig. 2.

Detailed Description

Hereinafter, the present invention will be described in more detail with reference to the accompanying drawings. In this specification, the same or similar reference numerals are given to the same or similar structures even in embodiments different from each other, and the description is replaced with that of the first time. As used in this specification, the singular forms "a", "an", and "the" include plural referents unless the context clearly dictates otherwise. The suffixes "module" and "section" of the constituent elements used in the following description are given or mixed for convenience of writing the present specification, and do not have meanings or functions different from each other.

FIG. 1 is a perspective view of the bottom of a piston of an extraction cassette for nucleic acid isolation according to an embodiment of the present invention, and FIG. 2 is a plan view of a lower body of the piston according to an embodiment of the present invention.

The plunger shown in FIG. 1 is attached to the extraction cassette shown in FIGS. 3 to 5, and the sample and the plurality of reagents disposed in the chamber of the extraction cassette are sequentially aspirated and mixed to extract nucleic acids.

As shown, the piston 300 may include an upper body 310, a lower body 320, a rotation adjustment module 330, a control rod module, and the like.

The upper body 310 is cylindrical and may include a hollow.

A control rod module that can move up and down along the hollow is disposed in the hollow of the upper body 310.

The control rod module may include: a coupling portion 301 coupled to a control rod of the nucleic acid extracting apparatus; and a contact portion 302 surrounding the outer periphery of the coupling portion 301 and moving to contact the inner wall of the upper body 310.

A coupling structure engaged with the lower body 320 may be formed at one end of the upper body 310, and a first hole connected to a fluid port of the lower body 320 and a second hole connected to a filter port of the lower body 320 may be formed. The second hole may be formed in a smaller diameter than the filter placing space 323 of the filter interface so as to prevent the support structure and the filter from being detached.

The lower body 320 may be combined with one end of the upper body 310. More specifically, the lower body 320 is fixed to a coupling structure formed at one end of the upper body 310 in an engaged manner.

The lower body 320 may include: the body is disc-shaped; a shaft 324 formed to protrude outward from the center of the body; and a liquid port 321 and a filter port 322 which are arranged to be spaced apart from the center of the body by the same distance. The body may be formed such that the central portion 320a protrudes more than the peripheral portion 320 b. The central portion 320a may be fixed by being inserted into a groove formed on the coupling structure of the upper body.

The liquid port 321 is used when the sample and the reagent are sucked, mixed, and discharged from the inside of the piston, and the filter port 322 is used when the nucleic acid trapping filter is cleaned or the nucleic acid is separated in the nucleic acid trapping filter.

Further, a groove depressed in the center direction may be formed on the outer circumference of the lower main body 320. The grooves can remove vacuum that may occur when liquid moves inside the extraction cartridge.

The liquid port 321 and the filter port 322 are arranged at a predetermined angle on the same circumference. For example, the filter interface 322 and the liquid interface 321 may be arranged so as to be spaced apart from each other by 18 ° to 36 °. More specifically, the two interfaces can be configured in such a manner as to form an interval of 22.5 °. In the case of using a stepping motor that performs one rotation by being divided into 16 times, the positions of the liquid interface 321 and the filter interface 322 can be changed by driving once.

The filter interface 322 of the lower body 320 may include a filter placing space 323 in which a filter and a support structure may be disposed. The filter for trapping nucleic acid may use a glass fiber filter having various particle sizes, and the support structure functions to fix the filter for trapping nucleic acid.

The support structure is made of a porous plastic material having a predetermined particle size so as to prevent the filter from being detached and maintain a predetermined pressure when the liquid is discharged.

The rotation adjusting module 330 is connected to a driving part of the device to perform a function of a medium for rotating the piston 300 by a predetermined angle.

The rotation adjustment module 330 may include: a coupling groove formed at a center portion of one surface of the rotation adjusting module 330 to be engaged with the shaft 324; and a driving groove formed on the other surface of the rotation regulating block 330 so as to engage with a driving shaft of the nucleic acid extracting apparatus.

The rotation adjustment module 330 positions the filter interface and the fluid interface at the appropriate reagent chamber interface locations so that the various chemical reactions required in the nucleic acid extraction step can be performed inside one extraction cartridge by engagement with the piston 300.

The liquid port is spaced apart from the filter port by a predetermined angle, and the rotation regulating module 330 rotates a plurality of the ports to positions suitable for respective steps when extracting nucleic acids.

After inserting a filter necessary for nucleic acid trapping to the filter interface, the lower body 320 is combined with the upper body 310, and then, the setting of the extraction cartridge is completed by combining with the first and second bodies 100 and 200.

Hereinafter, the structure of the extraction cartridge in which the piston as described above is installed will be specifically described with reference to fig. 3 to 5.

Fig. 3 is a perspective view of an extraction cassette used in the present invention, fig. 4 is a bottom perspective view of the extraction cassette used in the present invention, and fig. 5 is an exploded view of the extraction cassette shown in fig. 3.

Referring to fig. 3 to 5, the nucleic acid extraction cartridge may generally include a first body 100, a second body 200, a piston 300, a nucleic acid amplification module 400, and the like.

The first body 100 may be used for storing a plurality of reagents.

As shown, a plurality of chambers 110 may be formed in the first body 100, and the plurality of chambers 110 form compartments separated from each other. Reagents or samples different from each other are disposed in each chamber 110, and the chambers 110 form independent spaces so that the plurality of reagents are not mixed with each other.

The second body 200 includes a path for moving the reagent or the sample stored in the first body 100.

According to an embodiment of the present invention, the second body 200 may include a liquid flow path for moving liquid and an air flow path for moving air, and a pad 220 disposed on an upper surface thereof in order to prevent leakage of liquid when the second body 200 is coupled to the first body 100. If the pad plate 220 is coupled to the second body 200 of the extraction cartridge, the liquid flow path and the air flow path of the second body 200 of the extraction cartridge are blocked by the pad plate 220 to form a space, thereby forming the complete flow path 210.

By connecting the liquid channel to the first body 100, a space in which the sample and the reagent can move or mix is provided.

The air flow path serves to connect the amplification module to a vacuum control portion of the piston 300, to control a vacuum that may be generated when the extracted nucleic acid moves to the amplification module, and to prevent contamination of the nucleic acid amplification product.

A plurality of holes may be formed in the pad plate 220 to penetrate the pad plate 220 in the up-down direction. The liquid flow path and the air flow path in the lower part of the cartridge are connected to the plurality of reagent chambers 110 in the first body 100 of the cartridge through a plurality of holes.

The center portion of the pad plate 220 is combined with the bottom surface of the piston lower body 320 in a snug manner.

As the piston rotates, a plurality of holes formed at the central portion of the pad plate 220 overlap with the filter port or the liquid port of the piston lower body 320.

More specifically, a plurality of flow paths 210 may be formed at an upper portion of the second body 200. Each flow path 210 is formed to extend from the central portion to the peripheral portion of the second body 200 without crossing each other. As shown, one end of a part of the flow paths may be arranged on the same circumference, and the other end may be arranged on the same circumference.

An upper portion of the second body 200 may be combined with the pad 220.

A placing part 201 depressed deeply toward the lower end may be formed at the upper portion of the second body 200, and the setting plate 220 may be engaged with the placing part 201 at the upper portion of the second body 200 in a meshing manner.

The gasket 220 is closely attached to the upper surface of the second body 200 and can seal the plurality of flow paths 210. The pad plate 220 may be formed of rubber or synthetic resin having elasticity so that the pad plate 220 can be more strongly adhered to the second body 200.

According to an embodiment of the present invention, the plurality of holes are arranged to vertically overlap end portions of the plurality of channels 210. In other words, the plurality of holes formed in the pad plate 220 are paired with each other and connected by the flow path 210.

The pad 220 may include a plurality of holes 221 arranged on the same circumference C1 of the central portion and a plurality of holes 221 arranged on the same circumference C2 of the peripheral portion.

The piston 300 may be composed of a piston upper body 310 and a piston lower body 320.

An internal space in which a reagent and a sample can be mixed is formed in the upper body 310 of the piston 300, and a control rod module of the piston 300 that is movable in the vertical direction is disposed in the internal space.

The piston control rod module may include: a binding part 301 that binds to a drive part of the nucleic acid isolation apparatus; and a contact portion 302 which is in contact with the piston internal space and moves in the vertical direction.

The piston lower main body 320 is formed as one body by being combined with the piston upper main body 310.

The piston lower body 320 may be combined with a rotation regulation module 330.

As shown, the piston upper body 310 is inserted into a hole formed at the central portion of the first body 100, and the shaft 324 of the piston lower body 320 is inserted into a hole formed at the central portion of the second body 200.

The shaft 324 of the piston lower body 320 is engaged with and fixed to a rotation adjusting module 330, and the rotation adjusting module 330 is combined with the lower end of the second body 200.

The nucleic acid amplification module 400 may be combined with the first body 100 or the second body 200.

The internal channel 210 may be formed inside the nucleic acid amplification module 400, and one end of the internal channel 210 may be formed to overlap at least one of the plurality of channels 210 formed in the second body 200.

According to an embodiment of the present invention, the nucleic acid amplification module 400 may be provided with a fixing member 410, and the fixing member 410 may cover the nucleic acid amplification module 400 and be coupled to the first body 100 and the second body 200 so as not to allow the nucleic acid amplification module 400 to be freely separated.

The first body 100 may include a plurality of reagent chambers 110, each of the chambers 110 being formed in a spaced-apart manner from each other.

An interface overlapping one end of the flow path 210 or a plurality of holes formed in the pad plate 220 is formed at the lower end of each chamber 110. The ports may be formed to have different radii from each other according to the use of the chamber 110.

Each reagent chamber 110 includes a single reagent chamber fluid interface. Each reagent chamber liquid port is connected to a liquid flow path in the lower part of the extraction cartridge via a liquid port connection hole of the rubber pad 220. The sample chamber interface and the main mixing bead chamber interface are combined with separate sample and main mixing bead chambers 111, respectively.

The sample chamber ports may be arranged on the same circumference, and the main mixing bead chamber ports may be arranged on other circumferences.

The sample chamber may include a plurality of dry beads required for sample extraction, and the main mixing bead chamber may include a plurality of dry beads required for nucleic acid amplification.

The sample chamber and the main mixing bead chamber are connected to a sample chamber port and a main mixing bead chamber port, respectively, and each port is connected to a hole formed in the pad and a flow path of the second body 200 of the extraction cartridge to form a structure in which liquid can move.

According to the plunger of the cartridge for nucleic acid isolation as described above, the upper body for mixing the sample and the reagent and the lower body for sucking and discharging the reagent can be separated from each other, thereby simplifying the structure of the plunger. The manufacturing and assembling processes of the piston can be effectively performed.

Further, the reagent suction step and the reagent discharge step can be sequentially performed by the rotation of the piston by providing the liquid port and the filter port disposed at a predetermined angle from each other in the piston lower main body. This can improve the efficiency of the nucleic acid extraction step.

The nucleic acid extraction cassette described above is not limited to the structure and method described in the above embodiments, and various modifications can be made to the above embodiments by selectively combining all or a part of the respective embodiments.