阅读说明：本技术 一种含肝素钠酶解液的组合物及其制备方法和应用 (Composition containing heparin sodium enzymolysis liquid and preparation method and application thereof ) 是由 方秋华 魏海涛 吴荔琴 于钦秀 张炳顺 刘初亮 刘铄佳 刘佳华 刘琴发 于 2020-06-05 设计创作，主要内容包括：本发明属于肝素钠产品技术领域,具体涉及一种含肝素钠酶解液的组合物及其制备方法和应用。该组合物以肝素钠酶解液作为溶剂,加入蛋白质、糖类等搭配合理的营养成分,再利用葡萄糖酸内酯将组合物凝固成半固体状态,养殖动物摄入后可以显著提高养殖动物的采食量和体重,降低料肉比和腹泻率,并且半固体状较为容易储存、运输和使用,在放置7天有效成分含量基本不变,且能保持较好的营养保健作用,不会腐败变臭,稳定性好。(The invention belongs to the technical field of heparin sodium products, and particularly relates to a composition containing heparin sodium enzymatic hydrolysate and a preparation method and application thereof. The composition takes heparin sodium enzymolysis liquid as a solvent, protein, saccharide and other reasonable nutritional ingredients are added, the composition is solidified into a semisolid state by utilizing glucolactone, the feed intake and the body weight of cultured animals can be obviously improved after the composition is ingested by the cultured animals, the feed conversion ratio and the diarrhea rate are reduced, the semisolid state is easy to store, transport and use, the content of active ingredients is basically unchanged after the composition is placed for 7 days, the composition can keep a good nutritional health-care effect, does not decay and become smelly, and has good stability.)

1. The composition containing the heparin sodium enzymolysis liquid is characterized in that each kilogram of the composition contains 50-150 g of protein powder, 20-100 g of saccharides, 1-4 g of gluconolactone and 1-4 g of preservatives, and the heparin sodium enzymolysis liquid is used as a solvent to complement the weight.

2. The composition according to claim 1, wherein each kilogram of the composition contains 50-100 g of protein powder, 30-80 g of saccharides, 1-3 g of gluconolactone and 1-2 g of preservative, and the heparin sodium enzymolysis solution is used as a solvent to make up the weight.

3. The composition according to claim 1 or 2, wherein the heparin sodium enzymolysis solution is obtained by adding alkaline protease into small intestine mucosa for enzymolysis during extraction of heparin sodium and extracting heparin sodium.

4. The composition according to claim 3, wherein the alkaline protease is added in an amount of 0.5 to 1% by weight based on the weight of the mucous membrane of the small intestine.

5. The composition of claim 1 or 2, wherein the gluconolactone is glucono-lactone.

6. The composition as claimed in claim 1 or 2, wherein the protein powder is selected from one or more of soy protein, casein protein, whey protein, pea protein.

7. The composition according to claim 1 or 2, wherein the saccharide is selected from one or more of glucose, sucrose and lactose.

8. The composition according to claim 1 or 2, wherein the preservative is selected from one or two of sodium benzoate and potassium sorbate.

9. A process for the preparation of a composition according to any one of claims 1 to 8, characterized in that it comprises the following steps:

s1, mixing and stirring the protein powder, the saccharides, the gluconolactone and the preservative uniformly to obtain a uniform mixture;

s2, heating the heparin sodium enzymatic hydrolysate to 80-100 ℃, adding the uniform mixture obtained in the step S1, directionally and uniformly stirring, standing for 3-8 min, and cooling and condensing to obtain the heparin sodium enzymatic hydrolysate.

10. Use of the composition according to any one of claims 1 to 8 for the preparation of livestock and poultry feed, nutritional additives and veterinary drugs.

Technical Field

The invention belongs to the technical field of heparin sodium products. More particularly, relates to a composition containing heparin sodium enzymolysis liquid, a preparation method and application thereof.

Background

Heparin sodium is a mucopolysaccharide sulfate anticoagulant substance extracted from animal tissues, particularly small intestines of pigs, is the most effective anticoagulant medicament with the largest clinical dosage in the world, and is mainly applied to cardiovascular and cerebrovascular diseases and hemodialysis treatment. In addition, clinical application and research show that the heparin sodium also has other various biological activities and clinical applications, such as the function of reducing blood fat, resisting mesolamella smooth muscle cell proliferation, promoting fibrinolysis and the like, and has wide application prospect.

At present, a relatively common heparin sodium extraction method is an ion exchange resin method, specifically, the heparin sodium is obtained by carrying out steps of crushing treatment, enzymolysis extraction, resin adsorption, washing desorption, alcohol precipitation drying and the like on small intestines of pigs, wherein in the enzymolysis extraction process, protease needs to be added, the yield of the heparin sodium is relatively high, but protein can be decomposed, and a large amount of enzymolysis waste liquid is generated. The heparin sodium enzymolysis liquid has certain protein, amino acid, calcium, phosphorus and other major and trace elements, but has low nutrient content, is liquid, is difficult to recycle or transport, and increases the recycling cost; the direct discharge can cause the mass propagation of microorganisms in the water body, the reduction of dissolved oxygen, the influence on the growth of aquatic organisms, the putrefaction and the odor after long-term storage, and the serious influence on the living environment of people.

In order to provide a better treatment method of heparin sodium enzymolysis liquid, Chinese patent application CN110292110A discloses a compound aqueous solution containing heparin sodium enzymolysis waste liquid and vitamins, the compound aqueous solution is prepared by fat-soluble vitamins, water-soluble vitamins, an emulsifier and heparin sodium enzymolysis waste liquid, and the compound aqueous solution can be administrated in a drinking water mode, is applied to the aspects of livestock and poultry feeds, veterinary drugs and the like, ensures the rapid growth of livestock and poultry, and reduces the morbidity of the livestock and poultry; the Chinese patent application CN107751573A discloses a compound amino acid solution containing heparin sodium enzymolysis liquid waste, which is prepared from amino acid, traditional Chinese medicine extract and heparin sodium enzymolysis liquid waste, and can fully exert the synergistic effect of the amino acid and the traditional Chinese medicine extract and promote the growth of animals. However, the two treatment methods are to keep the heparin sodium enzymolysis liquid in a liquid state, the heparin sodium enzymolysis liquid is unstable after being placed for a long time, the problems of precipitation, peculiar smell, reduction of the content of effective components and the like of the solution can be caused, and the transportation is very inconvenient.

Disclosure of Invention

The invention aims to solve the technical problems of overcoming the defects and shortcomings of liquid state, instable long-term storage, inconvenient transportation and the like of the heparin sodium enzymatic hydrolysate in the conventional heparin sodium enzymatic hydrolysate treatment method, and providing a composition containing the heparin sodium enzymatic hydrolysate, which has rich nutrition, forms a stable semisolid state and is convenient to use and transport.

The invention aims to provide a composition containing heparin sodium enzymolysis liquid.

It is another object of the present invention to provide a process for the preparation of said composition.

It is another object of the present invention to provide the use of said composition.

The above purpose of the invention is realized by the following technical scheme:

a composition containing heparin sodium enzymolysis liquid comprises 50-150 g of protein powder, 20-100 g of saccharides, 1-4 g of gluconolactone and 1-4 g of preservative per kilogram of composition, and the heparin sodium enzymolysis liquid is used as a solvent for complementing weight.

Preferably, each kilogram of the composition contains 50-100 g of protein powder, 30-80 g of saccharides, 1-3 g of glucolactone and 1-2 g of preservative, and the heparin sodium enzymolysis liquid is used as a solvent to complement the weight.

More preferably, each kilogram of the composition contains 100g of albumen powder, 60g of saccharides, 2g of glucolactone and 2g of preservatives, and the heparin sodium enzymolysis liquid is used as a solvent to complement the weight.

In practical application, a large amount of drinking water in a farm is generally placed in a container at one time and taken as water for 1-2 days, but when the heparin sodium enzymolysis liquid is added with other nutrient components to be drunk by breeding animals in a liquid state, the stability is poor, the phenomena of precipitation, peculiar smell, putrefaction, reduction of the content of effective components and the like can occur after the heparin sodium enzymolysis liquid is placed for a period of time, the existing preparation is needed, and the transportation and the storage are also very inconvenient. The inventor discovers through a great deal of creative work that the heparin sodium enzymolysis liquid is used as a solvent, protein, saccharide and other reasonable nutritional ingredients are added, the composition is solidified into a semisolid state by utilizing glucolactone, the feed intake and the body weight of the cultured animals can be obviously improved after the cultured animals take the composition, the feed conversion ratio is reduced, the diarrhea rate is obviously reduced, the semisolid state is easy to store, transport and use, the content of the effective ingredients is basically unchanged after the semisolid state is placed for 7 days, the good nutritional health-care effect can be kept, the composition is not rotten and smelly, and the stability is good.

Further, the heparin sodium enzymolysis liquid is obtained by adding alkaline protease into small intestine mucosa for enzymolysis during extraction of heparin sodium and extracting heparin sodium.

Further, the alkaline protease is added in an amount of 0.5 to 1% by weight based on the weight of the mucous membrane of the small intestine.

Further, the gluconolactone is glucono-lactone.

Still further, the protein powder is selected from one or more of soy protein, casein, whey protein and pea protein.

Further, the saccharide is selected from one or more of glucose, sucrose and lactose.

Still further, the preservative is selected from one or two of sodium benzoate and potassium sorbate.

In addition, the invention also provides a preparation method of the composition, which comprises the following steps:

s1, mixing and stirring the protein powder, the saccharides, the gluconolactone and the preservative uniformly to obtain a uniform mixture;

s2, heating the heparin sodium enzymatic hydrolysate to 80-100 ℃, adding the uniform mixture obtained in the step S1, directionally and uniformly stirring, standing for 3-8 min, and cooling and condensing to obtain the heparin sodium enzymatic hydrolysate.

In addition, the invention also provides application of the composition in preparation of livestock and poultry feed, nutritional additives and veterinary drugs. Is especially suitable for young animals.

The invention has the following beneficial effects:

the composition containing the heparin sodium enzymatic hydrolysate takes the heparin sodium enzymatic hydrolysate as a solvent, protein, saccharides and other reasonable nutritional ingredients are added, the composition is solidified into a semi-solid state by utilizing glucolactone, the feed intake and the body weight of cultured animals can be obviously improved after the cultured animals take the composition, the feed conversion ratio and the diarrhea rate are reduced, the semi-solid state is easy to store, transport and use, the content of active ingredients is basically unchanged after the composition is placed for 7 days, the good nutritional health-care effect can be kept, the composition is not corrupted and smelly, and the stability is good; the preparation method is simple, the cost is low, a new heparin sodium enzymolysis liquid treatment method with more economic benefits is provided, and the method is suitable for industrial production.

Detailed Description

The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.

Wherein, the heparin sodium enzymolysis liquid is obtained by adding alkaline protease with the weight of 1 percent of that of small intestine mucosa into the small intestine mucosa for enzymolysis during the extraction of the heparin sodium and extracting the heparin sodium; or heparin sodium enzymolysis liquid in the conventional heparin sodium production process, in particular to heparin sodium enzymolysis liquid obtained by extracting heparin sodium from small intestine mucosa through the conventional enzymolysis extraction process and the resin adsorption process and the like.

The remaining reagents and materials used in the following examples are all commercially available unless otherwise specified.