阅读说明：本技术 一种改善细胞成骨性能的医疗植入物及其制备方法 (Medical implant for improving cell osteogenesis performance and preparation method thereof ) 是由 管迎春 崔智铨 于 2020-05-25 设计创作，主要内容包括：本发明公开了一种改善细胞成骨性能的医疗植入物及其制备方法,将打磨清洗后的医疗金属通过激光加工制得具有超亲水性能的表面结构,在所述的超亲水表面上制备他汀类药物涂层,可使药物负载更加均匀、充分,本发明的具有药物涂层的医疗植入物具有增强人体细胞成骨性能的功能,有利于植入物植入体内后与人体组织迅速紧密结合,有利于移植手术的顺利进行,有效避免移植失败带来的二次伤害。(The invention discloses a medical implant for improving cell osteogenesis performance and a preparation method thereof, wherein a medical metal after polishing and cleaning is processed by laser to prepare a surface structure with super-hydrophilic performance, and a statin drug coating is prepared on the super-hydrophilic surface, so that drug loading is more uniform and sufficient.)

1. A medical implant for improving osteogenic properties of cells, comprising: the surface of the medical implant is processed by femtosecond laser, the medical implant has super-hydrophilic performance, and the statin drug coating is prepared on the super-hydrophilic surface, so that the drug loading is more uniform and sufficient. The medical implant with the drug coating has the function of enhancing the cell osteogenesis performance of a human body, and is favorable for the rapid and close combination of the implant and human tissues after the implant is implanted into the body.

2. A medical implant for improving osteogenic properties of cells, according to claim 1, wherein said implant further comprises: the implant material is magnesium-based alloy, titanium-based alloy, stainless steel and other materials which can be used for biomedical materials.

3. A medical implant for improving osteogenic properties of cells, according to claim 1, wherein said implant further comprises: the statins comprise lovastatin, simvastatin, pravastatin, fluvastatin, cerivastatin, atorvastatin and the like.

4. A medical implant for improving osteogenic properties of cells, according to claim 1, wherein the method of preparation comprises the steps of:

grinding and polishing the surface of a medical implant material, and cleaning the surface by using an oil-removing cleaning agent;

sweeping the cleaned medical metal material in the step (1) by using ultrafast laser processing equipment to obtain a superhydrophilic structure on the surface of the material, wherein the processing process is carried out under the protection of inert gas;

and (3) soaking the biological implant material which is subjected to laser treatment and contains the super-hydrophilic surface in a statin solution with the concentration of 1mMol/L, taking out a sample after 1 minute, and drying the sample until the surface of the material is completely dried to obtain the medical implant containing the statin coating and capable of improving the osteogenesis performance.

5. The method for preparing a medical implant for improving osteogenic properties of cells according to claim 4, wherein the laser parameters set in the step (2) are: the laser wavelength is 193nm-1070nm, the laser pulse width is 50fs-500ps, the laser power is 0.5W-100W, and the laser pulse frequency is 1kHz-1 MHz.

6. The method for preparing a medical implant for improving osteogenesis performance of cells according to claim 4, wherein the step (2) uses galvanometer scanning to scan the laser on the surface of the metal material at a speed of 0.2mm/s to 3000mm/s, with a scanning interval of 10 μm to 200 μm and with a scanning frequency of 5 to 100 times.

Technical Field

The invention relates to a medical implant for improving cell osteogenesis performance and a preparation method thereof, belonging to the technical field of laser surface processing of medical materials.

Background

In the advanced medical field, the implantation of medical instruments has become one of the most effective means for treating orthopedic diseases. As the population ages, the market demand for high performance medical implants continues to increase. However, orthopedic implants also present some problems: the implant and the human bone interface are difficult to be completely combined, so that serious problems of prosthesis loosening, bone dissolution and the like can be caused after the prosthesis is implanted, the incidence rate of prosthesis loosening is up to 10-25%, and the failure of the implantation operation is caused. At present, the main population who receives the bone grafting operation is the old, and the failure of the bone grafting operation can cause serious secondary damage to the old.

Statins have recently been found to aid in bone remodeling. The expression of a bone morphogenetic protein-2 (BMP-2) gene is regulated by reducing the production of mevalonic acid which affects both bone resorption and bone formation, and the differentiation of adipocytes of bone marrow stromal stem cells is inhibited to osteoblasts to stimulate bone formation. Because statins have the function of regulating bone metabolism, inflammation, angiogenesis and the like, statins are often used for treating bone metabolism diseases such as osteoporosis and the like.

Superhydrophilicity refers to the phenomenon of a water droplet having a contact angle on a surface of no more than 5 °. The super-hydrophilic structure can enable the drug solution to be stably loaded on the surface of the implant to form a uniform drug coating, and the drug coating plays a role after the medical prosthesis is implanted into a body, so that the aim of increasing the bonding strength of the implant and the bone tissue is fulfilled. The preparation method of the super-hydrophilic surface structure mainly comprises a template method, electrochemical deposition, etching, sol-gel and the like. However, many of these methods have their limitations, such as involving hazardous chemicals, complicated procedures, low processing efficiency, and large dependence on the kind, size, and shape of the material. In recent years, the rapid development of ultrafast lasers has provided new powerful tools for the fabrication of surface micro-and nanostructures. The micro-structure is formed on the surface of the metal by laser treatment, and the hydrophilic property can be realized. As a substitute scheme, the ultrafast laser surface processing technology has become a good afterthought for micro-nano structure processing because of the advantages of high efficiency, flexibility, non-contact, no pollution, small heat affected zone, high controllability, no dependence on base materials and the like, and a series of outstanding research results are obtained in the aspect of preparation of the superhydrophilic surface.

Disclosure of Invention

The invention aims to provide a medical implant for improving the cell osteogenesis performance and a preparation method thereof, which can be widely applied to the surface treatment of metal medical implants to ensure that the implants are more tightly combined with human tissues.

In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: the medical implant has the surface which is processed by femtosecond laser and has super-hydrophilic performance, and a statin drug coating is prepared on the super-hydrophilic surface, so that the drug loading is more uniform and sufficient. The medical implant with the drug coating has the function of enhancing the cell osteogenesis performance of a human body, and is favorable for the rapid and close combination of the implant and human tissues after the implant is implanted into the body.

The implant material is magnesium-based alloy, titanium-based alloy, stainless steel and other materials which can be used for biomedical materials.

The statins comprise lovastatin, simvastatin, pravastatin, fluvastatin, cerivastatin, atorvastatin and the like.

The preparation method of the medical implant for improving the cell osteogenesis performance is shown in the flow chart of fig. 2 and mainly comprises the following steps:

(1) grinding and polishing the surface of the medical implant material, and cleaning by using an oil-removing cleaning agent;

(2) sweeping the cleaned medical metal material in the step (1) by using ultrafast laser processing equipment to obtain a superhydrophilic structure on the surface of the material, wherein the processing process is carried out under the protection of inert gas;

(3) the biological implant material which is processed by laser and contains the super-hydrophilic surface is soaked in a statin drug solution with the concentration of 1mMol/L, a sample is taken out after 1 minute, and the sample is dried until the surface of the material is completely dried, so that the medical implant which contains the statin drug coating and can improve the osteogenesis performance is obtained.

Wherein, the laser parameters set in the step (2) are as follows: the laser wavelength is 193nm-1070nm, the laser pulse width is 50fs-500ps, the laser power is 0.5W-100W, and the laser pulse frequency is 1kHz-1 MHz;

in the step (2), the laser is scanned on the surface of the metal material at a certain speed by using galvanometer scanning, wherein the scanning speed is 0.2-3000 mm/s, the scanning interval is 10-200 mu m, and the scanning frequency is 5-100 times.

The invention relates to a medical implant for improving cell osteogenesis performance and a preparation method thereof. Accelerating the postoperative recovery after the prosthesis is implanted. The invention adopts ultrafast laser to modify the metal surface of the implant, so that the implant has super-hydrophilic property, and the drug is more uniformly distributed on the surface of the implant.

The invention has the advantages that:

(1) the medical implant provided by the invention contains the statin coating on the surface, which is beneficial to reconstruction of implanted bone, improves osteogenesis activity and accelerates postoperative recovery.

(2) The surface of the medical implant material is processed by a laser direct writing system, so that the medical implant material has super-hydrophilic performance, and the drug coating on the surface of the material is more uniformly covered.

(3) The preparation method of the medical implant for improving the cell osteogenesis performance utilizes laser processing, can adapt to the processing requirements of various biological metal materials by changing laser parameters, has small processing heat influence, and can keep the original mechanical performance of a biological metal material matrix.

(4) The method has the advantages of high processing speed and flexible processing method, and is suitable for processing materials with various shapes and sizes.

Description of the drawings:

fig. 1 is a schematic view showing the construction of a medical implant for improving osteogenic properties of cells according to the present invention;

FIG. 2 is a flow chart of the method of the present invention;

FIG. 3 is a microscopic structure diagram of the super-hydrophilic structure prepared by the ultrafast laser according to the present invention;

FIG. 4 is a comparison of the contact angles of the superhydrophilic surface and the untreated titanium alloy surface of the titanium alloy samples made in the present invention with a lovastatin solution.

The specific implementation mode is as follows:

the invention is further described with reference to the following figures and specific examples.

As shown in the figure, the medical implant for improving the cell osteogenesis performance has the advantages that the surface of the medical implant is processed by femtosecond laser, the medical implant has super-hydrophilic performance, and the statin medicine coating is prepared on the super-hydrophilic surface, so that the medicine loading is more uniform and sufficient. The medical implant with the drug coating has the function of enhancing the cell osteogenesis performance of a human body, and is favorable for the rapid and close combination of the implant and human tissues after the implant is implanted into the body.

The implant material is magnesium-based alloy, titanium-based alloy, stainless steel and other materials which can be used for biomedical materials.

The statins comprise lovastatin, simvastatin, pravastatin, fluvastatin, cerivastatin, atorvastatin and the like.

The preparation method of the medical implant for improving the cell osteogenesis performance is shown in the flow chart of fig. 2 and mainly comprises the following steps:

(1) grinding and polishing the surface of the medical implant material, and cleaning by using an oil-removing cleaning agent;

(2) sweeping the cleaned medical metal material in the step (1) by using ultrafast laser processing equipment to obtain a superhydrophilic structure on the surface of the material, wherein the processing process is carried out under the protection of inert gas;

(3) the biological implant material which is processed by laser and contains the super-hydrophilic surface is soaked in a statin drug solution with the concentration of 1mMol/L, a sample is taken out after 1 minute, and the sample is dried until the surface of the material is completely dried, so that the medical implant which contains the statin drug coating and can improve the osteogenesis performance is obtained.

Wherein, the laser parameters set in the step (2) are as follows: the laser wavelength is 193nm-1070nm, the laser pulse width is 50fs-500ps, the laser power is 0.5W-100W, and the laser pulse frequency is 1kHz-1 MHz;

in the step (2), the laser is scanned on the surface of the metal material at a certain speed by using galvanometer scanning, wherein the scanning speed is 0.2-3000 mm/s, the scanning interval is 10-200 mu m, and the scanning frequency is 5-100 times.