3D printing super-elastic artificial bone and preparation method thereof
阅读说明：本技术 一种3d打印超弹性人工骨及其制备方法 (3D printing super-elastic artificial bone and preparation method thereof ) 是由 兰海 王晓东 龙旭东 于 2020-04-20 设计创作，主要内容包括：一种3D打印超弹性人工骨制备方法包括如下步骤：步骤1、将纳米羟基磷灰石粉末和聚己内酯颗粒在40℃的干燥箱内干燥24小时,备用；步骤2、采用熔融共混法制备纳米羟基磷灰石/聚己内酯共混物；步骤3、将共混物颗粒投放到3D打印线材专用挤出机加工成细丝,挤出温度为80℃,得到直径为1.75±0.2mm的3D打印线材；步骤4、用Solidworks软件设计骨组织工程支架模型；步骤5、制得具有良好力学性能及生物相容性的纳米羟基磷灰石/聚己内酯骨组织工程支架。(A preparation method of 3D printing super-elastic artificial bone comprises the following steps: step 1, drying the nano hydroxyapatite powder and the polycaprolactone particles in a drying oven at 40 ℃ for 24 hours for later use; step 2, preparing a nano hydroxyapatite/polycaprolactone blend by a melt blending method; step 3, putting the blend particles into a special extruder for the 3D printing wire rod to be processed into filaments, wherein the extrusion temperature is 80 ℃, and obtaining the 3D printing wire rod with the diameter of 1.75 +/-0.2 mm; step 4, designing a bone tissue engineering scaffold model by using Solidworks software; and 5, preparing the nano hydroxyapatite/polycaprolactone bone tissue engineering scaffold with good mechanical property and biocompatibility.)
1. A preparation method of 3D printing super-elastic artificial bone comprises the following steps:
step 1, drying the nano hydroxyapatite powder and the polycaprolactone particles in a drying oven at 40 ℃ for 24 hours for later use;
step 2, preparing a nano hydroxyapatite/polycaprolactone blend by a melt blending method;
firstly banburying for 5min at the rotating speed of 10r/min, weighing nano hydroxyapatite powder and polycaprolactone particles according to the proportion of 1:3, mechanically stirring and uniformly mixing, placing in a torque rheometer, blending for 8min at the rotating speed of 100r/min and the temperature of 160 ℃, and stopping when the torque is horizontal to obtain the nano hydroxyapatite/polycaprolactone blend;
step 3, putting the blend particles into a special extruder for the 3D printing wire rod to be processed into filaments, wherein the extrusion temperature is 80 ℃, and obtaining the 3D printing wire rod with the diameter of 1.75 +/-0.2 mm;
step 4, designing a bone tissue engineering scaffold model by using Solidworks software;
and 5, printing the bone tissue engineering scaffold with the computer-aided design by the obtained printing wire through a 3D printer, wherein the printing parameters of the 3D printer are set as follows: the nano hydroxyapatite/polycaprolactone bone tissue engineering scaffold with good mechanical property and biocompatibility is prepared at the printing temperature of 195 ℃, the inner diameter of a nozzle of 0.4mm, the printing air pressure of 0.4MPa and the printing speed of 10 mm/s.
2. A3D-printed superelastic artificial bone, comprising: the device comprises transverse extrusion structures (5), longitudinal extrusion structures (6) and interlayer connection points (7), wherein the transverse extrusion structures (5) are connected into unit layer stacks through the longitudinal extrusion structures (6), and the unit layer stacks are connected into a whole through the interlayer connection points (7).
3. The utility model provides a 3D prints artifical bone 3D printing apparatus of super-elasticity, includes chassis (1) and top surface welded door frame support (10), guide arm system frame (11) are installed to the front side of support (10), controller (2), its characterized in that are installed on the top of support (10): the top of support (10) is provided with material dish (3), the winding has on the middle concave surface of material dish (3) and is filiform hyperelastic bone material (30), the below of material dish (3) is provided with clearance bedplate (4), clearance bedplate (4) are L type structure and a side center is equipped with ring platform (40), the inside inseparable joint of ring platform (40) has bearing (41), the inside grafting of inner circle of bearing (41) has revolved post (42), a terminal surface symmetry of revolving post (42) is equipped with lug (420), lug (420) front end swing joint has clearance cover (43), high density sponge (430) have been cup jointed to the inside of clearance cover (43), hyperelastic bone material (30) with the inside grafting of high density sponge (430).
4. 3D printing superelastic artificial bone 3D printing apparatus according to claim 3, wherein: a printing head (110) is arranged on the X axis of the guide rod system frame (11), and the printing head (110) penetrates through the super-elastic bone material (30).
5. 3D printing superelastic artificial bone 3D printing apparatus according to claim 3, wherein: the porosity calculation system is characterized in that a porosity calculation system module (20) is installed on an inner main board of the controller (2), and a porosity adjusting button (21) is installed on the outer side face of the controller (2).
6. 3D printing superelastic artificial bone 3D printing apparatus according to claim 3, wherein: fixed lagging (31) has been cup jointed to the one end of material dish (3), fixed lagging (31) with support (10) pass through bolt fixed connection, the other end of material dish (3) is provided with spacing spiral cover (32).
7. 3D printing superelastic artificial bone 3D printing apparatus according to claim 1, wherein: the cleaning seat plate (4) is clamped at the edge of the support (10) and fixedly connected with the support through bolts.
8. 3D printing superelastic artificial bone 3D printing apparatus according to claim 3, wherein: the side face of the front end of each bump (420) is provided with a through hole (421), the center of one side of each cleaning sleeve (43) is provided with a lantern ring (431), and the lantern rings (431) are arranged between the two bumps (420) and are internally inserted with pins (44).
Technical Field
The invention relates to the technical field of orthopedic 3D printing, in particular to a 3D printing hyperelastic artificial bone and a preparation method thereof.
Background
The traditional chemical and physical method is adopted to manufacture the material bracket originally, the porosity prepared by the method can not be controlled, and the physicochemical property and the biocompatibility can not meet the clinical requirements. The porosity of the hyperelastic bone material can be controlled by a 3D printing technology, so that the printed hyperelastic bone material tissue has accurate porosity to meet clinical requirements. However, some low-mix 3D printing devices do not have a housing, and their material discs are also exposed, which can accumulate dust and affect the porosity of the printed product. In view of this, we propose a novel superelastic bone material 3D printing device.
Contributes to bone repair in patients with bone defects. For a long time, the repair of bone defects caused by wounds or tumors is not effectively solved, often leads to the disability or deformity of patients, seriously influences the life quality and physical and mental health of the patients, and is a common clinical treatment problem faced by the prior orthopedics department. The adoption of an effective bone grafting method is the key point for solving the problem. The hyperelastic bone material tissue engineering artificial bone is printed by 3D to treat bone defects, has mechanical properties similar to those of natural bone, can provide certain strength, and can effectively promote the formation of new bone. The artificial bone constructed by the method is expected to become an effective biological material for bone defect repair, the superelastic bone material tissue engineering artificial bone constructed based on the 3D printing technology is expected to become an effective treatment method for patients with bone defects, and the technology is expected to solve the problem of bone defect repair, so that the life quality of the patients is improved, and the huge economic burden of the patients and the families is effectively reduced.
Disclosure of Invention
The present invention is directed to a method for manufacturing the same, which solves the above problems of the prior art.
In order to achieve the purpose, the invention provides the following technical scheme:
a preparation method of 3D printing super-elastic artificial bone comprises the following steps:
step 1, drying the nano hydroxyapatite powder and the polycaprolactone particles in a drying oven at 40 ℃ for 24 hours for later use;
and 2, preparing the nano hydroxyapatite/polycaprolactone blend by adopting a melt blending method. Firstly banburying for 5min at the rotating speed of 10r/min, weighing nano hydroxyapatite powder and polycaprolactone particles according to the proportion of 1:3, mechanically stirring and uniformly mixing, placing in a torque rheometer, blending for 8min at the rotating speed of 100r/min and the temperature of 160 ℃, and stopping when the torque is horizontal to obtain the nano hydroxyapatite/polycaprolactone blend;
step 3, putting the blend particles into a special extruder for the 3D printing wire rod to be processed into filaments, wherein the extrusion temperature is 80 ℃, and obtaining the 3D printing wire rod with the diameter of 1.75 +/-0.2 mm;
step 4, designing a bone tissue engineering scaffold model by using Solidworks software;
and 5, printing the bone tissue engineering scaffold with the computer-aided design by the obtained printing wire through a 3D printer, wherein the printing parameters of the 3D printer are set as follows: the nano hydroxyapatite/polycaprolactone bone tissue engineering scaffold with good mechanical property and biocompatibility is prepared at the printing temperature of 195 ℃, the inner diameter of a nozzle of 0.4mm, the printing air pressure of 0.4MPa and the printing speed of 10 mm/s.
A3D-printed superelastic artificial bone, comprising: the device comprises
The utility model provides a 3D prints artifical bone 3D printing apparatus of super-elasticity, includes chassis 1 and its top surface welded
The
A porosity
Fixed
The cleaning seat plate 4 is clamped at the edge of the
The side surface of the front end of the convex block 420 is provided with a through hole 421, the center of one side of the cleaning sleeve 43 is provided with a lantern ring 431, and the lantern ring 431 is arranged between the two convex blocks 420 and is internally inserted with a pin 44.
Compared with the prior art, the invention has the beneficial effects that:
the invention has social benefit of making contribution to bone repair of patients with bone defects. For a long time, the repair of bone defects caused by wounds or tumors is not effectively solved, often leads to the disability or deformity of patients, seriously influences the life quality and physical and mental health of the patients, and is a common clinical treatment problem faced by the prior orthopedics department. The adoption of an effective bone grafting method is the key point for solving the problem. The hyperelastic bone material tissue engineering artificial bone is printed by 3D to treat bone defects, has mechanical properties similar to those of natural bone, can provide certain strength, and can effectively promote the formation of new bone. The artificial bone constructed by the invention is expected to become an effective biological material for bone defect repair, the superelastic bone material tissue engineering artificial bone constructed based on the 3D printing technology is expected to become an effective treatment method for patients with bone defects, and the technology is expected to solve the problem of bone defect repair, so that the life quality of the patients is improved, and the huge economic burden of the patients and the families is effectively reduced.
The 3D printing technology prepared by the invention has the characteristics of constructing the superelastic bone material tissue engineering artificial bone:
the biological compatibility is good: the material of the artificial bone is nontoxic and non-teratogenic, is beneficial to the adhesion and proliferation of seed cells, and degradation products have no toxic or side effect and do not cause inflammatory reaction and the like.
② has good biodegradability: the artificial bone can be degraded after the supporting function is finished, and the degradation rate is adapted to the growth rate of tissue cells.
③ has three-dimensional porous structure: the super-elastic bone material tissue engineering artificial bone constructed by the 3D printing technology has proper pore diameter, high porosity, higher internal specific surface area and interpenetration among pore canals, is beneficial to cell adhesion growth, blood vessel and nerve growth, and is also beneficial to nutrient infiltration and metabolite discharge.
Fourthly, the material has good plasticity: the super-elastic bone material tissue engineering artificial bone constructed by the 3D printing technology can be prefabricated into a specific shape according to the bone defect condition.
The material has certain mechanical strength: the artificial bone can provide support for the new tissue and maintain for a certain time until the new tissue has the own biomechanical characteristics.
Sixthly, the biocompatibility is good: the artificial bone is beneficial to cell adhesion and proliferation, and more importantly, can activate cell specific gene expression and maintain normal phenotype expression of cells.
The preparation technology of the invention is innovative: the hyperelastic bone material tissue engineering artificial bone is prepared by adopting a fusion blending and 3D printing technology, and the technology can directly form the required artificial bone at one time according to individual conditions by digital information processing of medical images and tissue structures. The artificial bone can provide more material choices, can realize the space multi-dimension and surface biological pore processing of the artificial bone, realizes light weight while solving the defects of the prior large solid, is closer to the biological characteristics, obtains better compatibility, further reduces the possibility of occurrence of complications, improves the postoperative life quality of patients, and has great application value. Compared with the traditional preparation method, the preparation technology of the artificial bone does not need organic solvents, can eliminate the toxicity and teratogenicity of liver and kidney induced by organic solvents such as chloroform and the like to the maximum extent, and overcomes the defects of solvent residue, difficult control of hole shape, complex preparation process, poor cell compatibility, difficult cell growth and adhesion and the like of the artificial bone prepared by the traditional method. And the corresponding bone tissue engineering scaffold can be manufactured according to the shape and the size of the bone defect, which is incomparable with other traditional manufacturing methods.
The innovation of the artificial bone is as follows: the artificial bone of the invention adopts nano hydroxyapatite/polycaprolactone as raw materials to prepare the bone tissue engineering scaffold, the nano hydroxyapatite has similar components with natural bone, has promotion effect on the secretion of bone growth enzyme and protein, and the polycaprolactone has better mechanical property. The prepared bone tissue engineering scaffold can integrate the advantages of two materials, and has better mechanical property and cell compatibility compared with the traditional scaffold.
According to the novel hyperelastic bone material 3D printing equipment and the process, the porosity of the printed hyperelastic bone material tissue is accurately controlled by controlling the controller provided with the porosity calculation system module and adjusting by using the porosity adjusting button; meanwhile, the cleaning seat plate is arranged below the material disc, the filamentous superelastic bone material penetrates through the cleaning sleeve, the superelastic bone material is continuously extruded out of the printing head along with printing of equipment, and therefore a printed product is free of impurities due to wiping of high-density sponge in the cleaning sleeve, and control of finished product porosity is facilitated.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic diagram of the controller of the present invention;
FIG. 3 is an exploded view of the material tray of the present invention;
FIG. 4 is a schematic view of the overall structure of the cleaning seat plate of the present invention;
fig. 5 is an exploded view of the cleaning seat plate of the present invention.
Fig. 6 is one of the schematic diagrams of the product of the present invention.
Fig. 7 is a second schematic diagram of the product of the present invention.
The meaning of the individual reference symbols in the figures is:
1. a chassis; 10. a support; 11. a guide rod system frame; 110. a print head; 2. a controller; 20. a porosity calculation system module; 21. a porosity adjustment button; 3. a material tray; 30. a super-elastic bone material; 31. fixing the sleeve plate; 32. limiting and screwing a cover; 4. cleaning the seat board; 40. a circular ring table; 41. a bearing; 42. rotating the column; 420. a bump; 421. a through hole; 43. Cleaning the sleeve; 430. a high density sponge; 431. a collar; 44. a pin. 5. A transverse extrusion structure; 6, longitudinal extrusion structure; 7. and connecting points between layers.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "center", "centerline", "longitudinal", "lateral", "length", "width", "thickness", "depth", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be taken as limiting the invention, also in the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
Referring to fig. 1-7, the present invention provides a technical solution:
A3D-printed superelastic artificial bone, comprising: the device comprises transverse extrusion structures (5), longitudinal extrusion structures (6) and interlayer connection points (7), wherein the transverse extrusion structures (5) are connected into unit layer stacks through the longitudinal extrusion structures (6), and the unit layer stacks are connected into a whole through the interlayer connection points (7).
The utility model provides a novel hyperelastic bone material 3D printing apparatus, in order to avoid having the impurity of particulate matters such as dust in the printed matter, the inventor has set up clearance bedplate 4. The printer comprises an underframe 1 and a door
In this embodiment, the cleaning seat plate 4 is made of PP material and has an integrally formed structure, which is light in weight, good in toughness and rigid in strength, so that it is durable. Wherein the cleaning sleeve 43 can be used for standby, the pin 44 is ejected, the cleaning sleeve 43 can be replaced, and the high-density sponge 430 in the cleaning sleeve is cleaned. Wherein the composition of the
Further, a
Specifically, a porosity
Specifically, a fixed
In addition, the cleaning seat plate 4 is clamped at the edge of the
It needs to be supplemented that the side surface of the front end of the convex block 420 is provided with a through hole 421, the center of one side of the cleaning sleeve 43 is provided with a lantern ring 431, the lantern ring 431 is arranged between the two convex blocks 420, and the inside of the lantern ring 431 is inserted with the pin 44, wherein the inner diameter of the lantern ring 431 is larger than the outer diameter of the pin 44, so that the cleaning sleeve 43 can swing back and forth between the two convex blocks 420.
Before printing, the novel hyperelastic bone material 3D printing equipment penetrates
A novel 3D printing process for super-elastic bone materials comprises the following steps:
step 1, drying the nano hydroxyapatite powder and the polycaprolactone particles in a drying oven at 40 ℃ for 24 hours for later use;
and 2, preparing the nano hydroxyapatite/polycaprolactone blend by adopting a melt blending method. Firstly banburying for 5min at the rotating speed of 10r/min, weighing nano hydroxyapatite powder and polycaprolactone particles according to the proportion of 1:3, mechanically stirring and uniformly mixing, placing in a torque rheometer, blending for 8min at the rotating speed of 100r/min and the temperature of 160 ℃, and stopping when the torque is horizontal to obtain the nano hydroxyapatite/polycaprolactone blend;
step 3, putting the blend particles into a special extruder for the 3D printing wire rod to be processed into filaments, wherein the extrusion temperature is 80 ℃, and obtaining the 3D printing wire rod with the diameter of 1.75 +/-0.2 mm;
step 4, designing a bone tissue engineering scaffold model by using Solidworks software;
and 5, printing the bone tissue engineering scaffold with the computer-aided design by the obtained printing wire through a 3D printer, wherein the printing parameters of the 3D printer are set as follows: 195 deg.C, 0.4mm inner diameter of the nozzle, 0.4MPa printing pressure and 10mm/s printing speed. The nano hydroxyapatite/polycaprolactone bone tissue engineering scaffold with good mechanical property and biocompatibility is prepared.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the preferred embodiments of the present invention are described in the above embodiments and the description, and are not intended to limit the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.
- 上一篇：一种医用注射器针头装配设备
- 下一篇：一种光固化3D打印后处理装置