阅读说明：本技术 昆布提取物的应用 (Application of thallus laminariae extract ) 是由 刘玉亮 邓静 于 2020-06-19 设计创作，主要内容包括：本发明属于化妆品技术领域,具体涉及一种昆布提取物的应用。一种昆布提取物的应用,昆布提取物采用如下方法制备得到：步骤1)去除昆布中的水分；步骤2)用有机试剂浸提并抽滤后,得到浸提液；步骤3)浓缩浸提液,除去沉淀得到滤液；滤液为昆布提取物的粗品；应用为昆布提取物在皮肤外用剂中的应用。本发明的昆布提取物应用于皮肤外用剂中,与普通的昆布提取物相比,在护肤品和化妆品中的稳定性提高。(The invention belongs to the technical field of cosmetics, and particularly relates to an application of a kelp extract. The application of the kelp extract comprises the following steps: step 1) removing water in the kelp; step 2), leaching with an organic reagent and carrying out suction filtration to obtain a leaching solution; step 3), concentrating the leaching liquor, and removing precipitates to obtain a filtrate; the filtrate is crude product of thallus laminariae extract; the application is the application of thallus laminariae extract in skin external preparation. The laminaria extract of the invention is applied to skin external preparations, and has improved stability in skin care products and cosmetics compared with the conventional laminaria extract.)

1. The application of the kelp extract is that the kelp extract is prepared by the following method:

step 1) removing water in the kelp;

step 2), leaching with an organic reagent and carrying out suction filtration to obtain a leaching solution;

step 3) concentrating the leaching liquor, and removing precipitates to obtain filtrate; the filtrate is crude product of thallus laminariae extract;

the method is characterized in that: the application is the application of the laminaria japonica extract in the skin external preparation.

2. The use of claim 1, wherein: further comprising the steps of:

step 4), crystallizing the filtrate at low temperature and filtering to obtain crystals which are the kelp oil-soluble extract;

step 5), extracting the filtrate obtained after filtering in the step 4) by using a fat-soluble solvent to obtain a fat-soluble solvent layer;

step 6), crystallizing the fat-soluble solvent layer at low temperature to separate out yellow-green solids, namely the kelp water-soluble extract;

the thallus laminariae extract is thallus laminariae oil soluble extract, or thallus laminariae water soluble extract, or mixture of thallus laminariae oil soluble extract and thallus laminariae water soluble extract.

3. Use according to claim 2, characterized in that:

in the step 1), kelp is frozen for 10-15 days at-80 to-5 ℃, air thawing is carried out at room temperature, and then the kelp is centrifuged at 500-8000 rpm to remove water in the kelp; repeating the steps for 1-4 times;

step 2) adding an organic reagent into the kelp, leaching for 1-24 hours in the dark, then carrying out suction filtration to obtain a leaching solution, repeatedly leaching the kelp until the leaching solution is colorless, and combining the leaching solutions; the organic reagent is acetone, methanol or 60-99V/V% ethanol water solution;

step 3) concentrating the leaching liquor at 15-40 ℃ until a large amount of precipitates appear in the solution, and filtering to remove the precipitates to obtain filtrate;

step 4), crystallizing the filtrate at the low temperature of 4-30 ℃ for 5-72 hours, and filtering to obtain crystals which are kelp oil-soluble extracts;

step 5) extracting the filtrate obtained after filtering in the step 4) for multiple times by adopting a fat-soluble solvent until a fat-soluble solvent layer is colorless, and combining the fat-soluble solvent layers obtained by multiple times of extraction; the fat-soluble solvent is one or more of dichloromethane, n-hexane and cyclohexane;

and 6) concentrating the fat-soluble solvent layer at low temperature of 0-30 ℃ until yellow-green solids are separated out from the solution, namely the kelp water-soluble extract.

4. Use according to claim 2, characterized in that: the use of the thallus laminariae extract as antioxidant and/or humectant of natural origin in skin external preparation is provided.

5. The use according to claim 4, wherein the laminaria japonica extract is contained in the skin external preparation in an amount of 0.0005-20 wt%, preferably 0.001-5 wt%.

6. The use according to claim 4, wherein the external preparation for skin is a cosmetic lotion, essence, cream or emulsion.

7. Use according to claim 2, characterized in that: the application is the application of the kelp extract as an antioxidant, an elastic fiber hydrolysis inhibitor and an agent for enhancing the skin barrier function in the skin external preparation.

8. The use according to claim 7, wherein the concentration of the laminaria extract in the antioxidant or the inhibitor of elastic fiber hydrolysis is preferably 1 μ g/mL to 500 μ g/mL, more preferably 100 μ g/mL.

9. The use of claim 7, wherein the concentration of the laminaria japonica extract in the agent for enhancing skin barrier function is preferably 1 μ g/mL to 500 μ g/mL, more preferably 100 μ g/mL.

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to an application of a kelp extract.

Background

Recent studies in cell biology and molecular biology have considered that skin aging is both necessary for human gene metabolism and affected by exogenous damage. Exogenous damage includes environmental radiation, air pollution, quality of diet, degree of fatigue, and the like.

At present, the theory of free radicals is widely accepted as a theory of delaying skin aging, namely: the natural ability of the human body to combat free radicals is constantly decreasing, the human cells are aging and the skin becomes rough. The elimination of excessive free radicals in the human body is mainly through an anti-oxidation effect. More than 95% of free radicals in human body are oxygen free radicals, and oxidation resistance is to inhibit the oxidation reaction of free radicals. Active oxygen free radicals are generated in the physiological metabolic process of the human body, and under the normal condition, an antioxidant defense system capable of maintaining the balance state between oxidation and antioxidation is arranged in the human body to slow down the generation of the active oxygen and the free radicals. However, ultraviolet radiation, air pollution, environmental pollutants such as chemical substances and the like in the environment can stimulate the human body to continuously generate free radicals, and the large amount of generated free radicals can reduce the antioxidant defense capability of the skin, cause the damage of the skin, and cause the skin aging phenomena such as dark lines, fine lines, wrinkles and the like.

Photoaging caused by UV exposure causes increased activity of skin elastase. Elastase is distributed in various tissues and cells, and the increase of elastase activity can cause the hydrolysis of skin elastic fibers, thereby accelerating the decomposition of collagen and elastin in dermis, leading the skin to lose elasticity, and causing skin aging phenomena such as wrinkles and the like.

The skin elasticity is closely related to the moisture content of the skin, when the horny layer is rich in moisture, the skin elasticity is better, and the horny layer is dried to cause the phenomena of skin elasticity reduction, skin dryness, skin tightness, skin cracking and the like. The moisturizing component has the effects of keeping skin moisture, delaying and preventing moisture volatilization, has important significance for maintaining and repairing skin barrier function and reducing skin, and the substance or the composition which is derived from natural plants and has the effects of moisturizing has the advantages of high safety, low cost and the like, and can be widely applied to cosmetics.

In addition, the tangle (Latin name: Ecklonia kurome Okam) is also called "Hecai", "Gymnema sylvestre", "Gymnema quinquefolium", etc. Phaeophyceae, Alariaceae. The artificial sea cucumber is grown in a cold sea and is attached to a rock reef in a depth of 1-3 meters below a main tide line. Distributed in Shandong, Liaoning, a coastal area. The records in the "sea medicine materia Medica" are: "the herb grows downstream and emerges as new Luo, leaves are fine, yellow and black, the mustache rubs the root of the beard into a rope, dries in the shade, comes from China on the ship. The herbaceous plants in the herbaceous part are listed in the compendium of materia medica, which have the efficacies of eliminating phlegm, softening hardness, dissipating stagnation, inducing diuresis and reducing edema, and are seaweed with high medicinal value.

Disclosure of Invention

The invention aims to solve the technical problems of human skin aging, skin elasticity reduction, easy occurrence of dryness, tightness, cracking and the like, and aims to provide application of a kelp extract.

The application of the kelp extract is that the kelp extract is prepared by the following method:

step 1) removing water in the kelp;

step 2), leaching with an organic reagent and carrying out suction filtration to obtain a leaching solution;

step 3) concentrating the leaching liquor, and removing precipitates to obtain filtrate; the filtrate is crude product of thallus laminariae extract;

the method is characterized in that: the application is the application of the laminaria japonica extract in the skin external preparation.

Further comprising the steps of:

step 4), crystallizing the filtrate at low temperature and filtering to obtain crystals which are the kelp oil-soluble extract;

step 5), extracting the filtrate obtained after filtering in the step 4) by using a fat-soluble solvent to obtain a fat-soluble solvent layer;

step 6), crystallizing the fat-soluble solvent layer at low temperature to separate out yellow-green solids, namely the kelp water-soluble extract;

the thallus laminariae extract is thallus laminariae oil soluble extract, or thallus laminariae water soluble extract, or mixture of thallus laminariae oil soluble extract and thallus laminariae water soluble extract.

In the step 1), kelp is frozen for 10-15 days at-80 to-5 ℃, air thawing is carried out at room temperature, and then the kelp is centrifuged at 500-8000 rpm to remove water in the kelp; repeating the steps for 1-4 times;

step 2) adding an organic reagent into the kelp, leaching for 1-24 hours in the dark, then carrying out suction filtration to obtain a leaching solution, repeatedly leaching the kelp until the leaching solution is colorless, and combining the leaching solutions; the organic reagent is acetone, methanol or 60-99V/V% ethanol water solution;

step 3) concentrating the leaching liquor at 15-40 ℃ until a large amount of precipitates appear in the solution, and filtering to remove the precipitates to obtain filtrate;

step 4), crystallizing the filtrate at the low temperature of 4-30 ℃ for 5-72 hours, and filtering to obtain crystals which are kelp oil-soluble extracts;

step 5) extracting the filtrate obtained after filtering in the step 4) for multiple times by adopting a fat-soluble solvent until a fat-soluble solvent layer is colorless, and combining the fat-soluble solvent layers obtained by multiple times of extraction; the fat-soluble solvent is one or more of dichloromethane, n-hexane and cyclohexane;

and 6) concentrating the fat-soluble solvent layer at low temperature of 0-30 ℃ until yellow-green solids are separated out from the solution, namely the kelp water-soluble extract.

The use of the thallus laminariae extract as antioxidant and/or humectant of natural origin in skin external preparation is provided.

The content of the laminaria japonica extract in the skin external preparation is 0.0005 wt% -20 wt%, preferably 0.001 wt% -5 wt%.

The skin external preparation is cosmetic water, essence, cream or lotion.

The application is the application of the kelp extract as an antioxidant, an elastic fiber hydrolysis inhibitor and an agent for enhancing the skin barrier function in the skin external preparation.

The concentration of the tangle extract in the antioxidant or the elastic fiber hydrolysis inhibitor is preferably 1. mu.g/mL-500. mu.g/mL, more preferably 100. mu.g/mL.

The concentration of the laminaria japonica extract in the agent for enhancing the skin barrier function is preferably 1 to 500. mu.g/mL, more preferably 100. mu.g/mL.

The positive progress effects of the invention are as follows: the kelp extract prepared by the preparation method provided by the invention contains the water-soluble components and the oil-soluble components of kelp simultaneously, is used as a natural antioxidant and a humectant, and is mainly applied to skin external agents, antioxidants, elastic fiber hydrolysis inhibitors and skin barrier function enhancement in the preparation of skin care products and cosmetics. The kelp extract is compounded with other substances, so that the oxidation resistance and the skin moisturizing capability of the skin can be adjusted.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further explained below.

The application of the kelp extract comprises the following steps:

step 1) removing water in the kelp.

Preferably, the kelp is frozen at the temperature of-80 to-5 ℃, is taken out after being frozen for 10 to 15 days, is unfrozen in air at room temperature until the central temperature of the kelp reaches 0 to 2 ℃, is subjected to a freeze-thaw process, is put into a centrifuge, is centrifuged at the rotation speed of 500 to 8000rpm for 5 to 15 minutes to remove moisture, and is repeated for 1 to 4 times.

And 2) leaching by using an organic reagent and performing suction filtration to obtain a leaching solution.

Preferably, the organic reagent is added into the kelp, leaching is carried out for 1 to 24 hours in the dark and then suction filtration is carried out to obtain leaching liquor, the kelp is repeatedly leached until the leaching liquor is colorless, and the leaching liquor is merged.

Wherein the organic reagent is acetone, methanol or 60V/V-99V/V% ethanol water solution.

Step 3), concentrating the leaching liquor, and removing precipitates to obtain a filtrate; the filtrate is crude thallus laminariae extract.

Preferably, the leaching liquor is concentrated at 15-40 ℃ until precipitation appears in the solution, and the precipitate is removed by filtration to obtain a filtrate.

And 4) crystallizing the filtrate at low temperature and filtering to obtain crystals of the kelp oil-soluble extract.

Preferably, the filtrate is crystallized at a low temperature of 4 to 30 ℃ for 5 to 72 hours, and filtered to obtain crystals of the oil-soluble extract of kelp.

And 5) extracting the filtrate obtained after filtering in the step 4) by using a fat-soluble solvent to obtain a fat-soluble solvent layer.

Preferably, the filtrate obtained after filtering in step 4) is extracted for multiple times by using a fat-soluble solvent until the fat-soluble solvent layer is colorless, and the fat-soluble solvent layers obtained by multiple extractions are combined;

wherein the fat-soluble solvent is one or more of dichloromethane, n-hexane and cyclohexane.

And 6) crystallizing the fat-soluble solvent layer at low temperature to separate out yellow-green solids, namely the kelp water-soluble extract.

Preferably, the fat-soluble solvent layer is concentrated at low temperature of 0-30 ℃ until yellow-green solid is separated out from the solution, namely the kelp water-soluble extract.

The application is the application of thallus laminariae extract in skin external preparation.

The thallus laminariae extract is crude thallus laminariae extract, or thallus laminariae oil soluble extract, or thallus laminariae water soluble extract, or mixture of thallus laminariae oil soluble extract and thallus laminariae water soluble extract.

Use of thallus laminariae extract as antioxidant and/or humectant of natural origin in skin external preparation is provided. The content of the Laminaria japonica extract in the skin external preparation is 0.0005 wt% -20 wt%, preferably 0.001 wt% -5 wt%. The skin external preparation is cosmetic water, essence, cream or lotion.

The application of thallus laminariae extract in skin external preparation as antioxidant, elastic fiber hydrolysis inhibitor, and skin barrier function enhancing agent is provided. The concentration of the tangle extract in the antioxidant or the elastic fiber hydrolysis inhibitor is preferably 1. mu.g/mL-500. mu.g/mL, more preferably 100. mu.g/mL. The concentration of the Laminaria japonica extract in the skin barrier function-enhancing agent is preferably 1. mu.g/mL-500. mu.g/mL, more preferably 100. mu.g/mL.