阅读说明：本技术 一种液氮组织研磨器 (Liquid nitrogen tissue grinder ) 是由 刘贤海 姚宏兵 杨鑫 于东寨 臧丽丽 于 2020-05-14 设计创作，主要内容包括：本发明公开了一种液氮组织研磨器,本液氮组织研磨器由研磨器本体和底座构成,研磨器本体包括研磨塞和研磨管,本研磨器可以实现冷冻样品与研磨样品一次性完成,简化了实验流程,同时极大地减少了液氮的使用量,降低了实验成本,装卸方便,操作简便安全,便携性强,造价低廉,适合大规模推广使用。(The invention discloses a liquid nitrogen tissue grinder which is composed of a grinder body and a base, wherein the grinder body comprises a grinding plug and a grinding pipe, the grinder can finish freezing samples and grinding samples at one time, the experimental process is simplified, the usage amount of liquid nitrogen is greatly reduced, the experimental cost is reduced, the liquid nitrogen tissue grinder is convenient to assemble and disassemble, simple, convenient and safe to operate, strong in portability and low in manufacturing cost, and is suitable for large-scale popularization and use.)

1. The liquid nitrogen tissue grinder is characterized by comprising a grinder body (1) and a base (2), wherein the grinder body (1) comprises a grinding plug (3) and a grinding pipe (4), the grinding plug (3) is a bolt (31) with a conical bottom, and the conical surface is a ground grinding surface (32); the outer part of the grinding pipe (4) is a cuboid with a square bottom surface, the inner cavity (41) is a cylinder, the bottom of the inner cavity is provided with a conical cavity (42) corresponding to the bottom of the grinding plug (3), the side wall of the inner cavity (41) is provided with a thread (43) corresponding to the grinding plug, and the length of the grinding plug (3) is equal to or greater than the depth of the inner cavity (41) of the grinding pipe; a square groove (21) is arranged on the base (2), the inner side length of the square groove is equal to the side length of the bottom surface of the grinding pipe (4), 2-4 screw holes (22) are formed in the base (2), and the grinder is made of stainless steel or ceramic or high-hardness resin.

2. The liquid nitrogen tissue grinder according to claim 1, wherein the conical cavity (41) is detachably connected with the grinding pipe (4) in a snap-in manner.

3. The liquid nitrogen tissue grinder according to claim 1, wherein a handle (33) is provided on the top of said grinding plug (3).

4. The liquid nitrogen tissue grinder according to claim 1, wherein the grinding tube (4) is preferably provided with a closable vent hole on the side wall of the upper end thereof.

Technical Field

The invention relates to the technical field of biological experiment devices, in particular to a liquid nitrogen tissue grinder.

Background

Liquid nitrogen milling is widely used in molecular biology experiments, particularly in relation to the separation of ribonucleic acid (RNA) and deoxyribonucleic acid (DNA). The liquid nitrogen has a temperature of-196 deg.C, which can make various tissue components not easily damaged or degraded, but also can make the tissue hard, and the brittleness is increased and easy to grind. Due to the extremely low temperature of liquid nitrogen, special care is taken in handling. Liquid nitrogen grinding is used for stopping all biological reactions inside and outside cells, such as RNA extraction, RNA enzyme degradation reaction prevention and the like; the other reason is that the cells in the liquid nitrogen are completely frozen and hard, the grinding can achieve a good cell breaking effect, and the cells are ground into powder to release substances inside.

Disclosure of Invention

The invention aims to provide a liquid nitrogen tissue grinder which can overcome the defects of the prior art.

The invention provides a liquid nitrogen tissue grinder which comprises a grinder body and a base, wherein the grinder body comprises a grinding plug and a grinding pipe, and the liquid nitrogen tissue grinder has the following specific structure: the grinding plug is a bolt with a conical bottom, and the conical structure at the bottom is a frosted surface; the outer part of the grinding pipe is a cuboid with a square bottom surface, the inner cavity is a cylinder, the bottom of the inner cavity is provided with a conical cavity corresponding to the bottom of the grinding plug, the side wall of the inner cavity is provided with a thread corresponding to the grinding plug, and the length of a bolt of the grinding plug is the same as or slightly greater than the depth of the inner cavity of the grinding pipe; the grinding plug is detachably connected with the top of the grinding pipe through threads; the base is provided with a square groove, the length of the inner side of the square groove is equal to or slightly larger than the side length of the bottom surface of the grinding pipe, and the base is provided with 2-4 screw holes which can be fixed on the operating table board through screws. The grinder is made of stainless steel or ceramic or high-hardness resin.

Preferably, the conical cavity structure at the bottom of the grinding pipe is in snap-in detachable connection.

Preferably, the grinding plug has a handle at the top.

Preferably, the side wall of the upper end of the grinding pipe is provided with a closable vent hole.

During the use, close the exhaust hole precooling on the grinding pipe, will wait to grind the sample and drop into the grinding pipe in, add liquid nitrogen to the half degree of depth of body in to the grinding pipe, twist and close the grinding stopper, place the grinder in low temperature environment freezing sample, take out after freezing, insert the base with the grinder, open the exhaust hole, the rotation handle makes the grinding stopper impel to the grinding pipe bottom, rotatory propulsion in-process sample is ground, the twist grip is ground completely to the sample, take off the grinding pipe from the base, open the circular cone cavity structure of grinding pipe bottom, it can to pour the sample after grinding.

The liquid nitrogen tissue grinder provided by the invention can ensure that a frozen sample and a ground sample are finished at one time, simplifies the experimental process, greatly reduces the using amount of liquid nitrogen, reduces the experimental cost, is convenient to assemble and disassemble, is simple, convenient and safe to operate, strong in portability and low in manufacturing cost, and is suitable for large-scale popularization and use.

Drawings

Fig. 1 is a structural diagram of a grinder body in embodiment 1, fig. 2 is a front view of a base in embodiment 1, fig. 3 is a sectional view of the base in embodiment 1, fig. 4 is a structural diagram of a grinding pipe in embodiment 1, and fig. 5 is a sectional view of the grinding pipe in embodiment 1.

In fig. 1, 1 is a grinder body, 3 is a grinding plug, 4 is a grinding pipe, and 33 is a handle; in fig. 2 and 3, 2 is a base, 21 is a groove, and 22 is a screw hole; in FIG. 4, 31 is a bolt, 32 is a grinding surface, and 33 is a handle; in fig. 5, 41 is an inner cavity, 42 is a conical cavity, and 43 is a thread.

Detailed Description

The present invention will be described in detail with reference to specific embodiments. The following examples are presented to assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any manner.