阅读说明：本技术 使用用于表达嵌合抗原受体(car)或转基因t细胞受体(tcr)的病毒载体转导细胞的方法 (Methods of transducing cells using viral vectors for expression of Chimeric Antigen Receptors (CARs) or transgenic T Cell Receptors (TCRs) ) 是由 R.贝斯威克 E.陈 C.佩蒂科尼 J.福克纳 E.科索普劳 M.普勒 于 2019-01-08 设计创作，主要内容包括：本发明提供了一种用病毒载体转导细胞的方法,该病毒载体包含编码针对靶抗原的嵌合抗原受体(CAR)或转基因T细胞受体(TCR)的核酸,其中细胞包含表达靶抗原的细胞亚群和不表达靶抗原的细胞亚群,该方法包括以感染复数(MOI)转导细胞的步骤,使得与表达靶抗原的细胞亚群相比,不表达靶抗原的细胞亚群以更大的程度被转导。本发明还提供了通过此类方法制备的细胞组合物及其在治疗和/或预防疾病中的用途。(The invention provides a method of transducing a cell with a viral vector comprising a nucleic acid encoding a Chimeric Antigen Receptor (CAR) or a transgenic T Cell Receptor (TCR) against a target antigen, wherein the cell comprises a subpopulation of cells that express the target antigen and a subpopulation of cells that do not express the target antigen, the method comprising the step of transducing the cell at a multiplicity of infection (MOI) such that the subpopulation of cells that do not express the target antigen is transduced to a greater extent than the subpopulation of cells that express the target antigen. The invention also provides cellular compositions prepared by such methods and their use in the treatment and/or prevention of disease.)