阅读说明：本技术 处理玻璃微珠的方法及其应用 (Method for treating glass beads and application thereof ) 是由 黄志刚 王秀柱 王丽 王伟权 刘金梁 于 2020-05-19 设计创作，主要内容包括：本文提供了一种处理玻璃微珠的方法,包括让所述玻璃微珠依次与重铬酸钾和甲基硅油接触。本文还提供了经该方法处理的玻璃微珠及其填充微柱的用途。本文提供的玻璃微珠处理方法操作简单,成本低廉,经处理的玻璃微珠透光性好,疏水力强,解决了玻璃微珠柱凝集卡生产过程中产生微小气泡的问题。(Provided herein is a method of treating glass microspheres comprising contacting the glass microspheres with potassium dichromate and methyl silicone oil in that order. Also provided herein are glass microspheres treated by the method and uses thereof to pack microcolumns. The glass bead processing method provided by the invention is simple to operate and low in cost, the processed glass beads are good in light transmittance and strong in hydrophobic force, and the problem of generation of micro bubbles in the production process of the glass bead column agglutination card is solved.)

1. A method of treating glass microspheres comprising contacting said glass microspheres with potassium dichromate and methyl silicone oil in that order.

2. The method of claim 1, comprising contacting the glass microspheres sequentially with:

1) a sulfuric acid solution containing potassium dichromate; and

2) petroleum ether solution containing methyl silicone oil.

3. The method according to claim 1 or 2, comprising washing the glass beads with water and anhydrous ethanol in this order after contacting the glass beads with the potassium dichromate, and then contacting the glass beads with the methyl silicone oil.

4. The method according to claim 1 or 2, comprising washing the glass microspheres with absolute ethanol and water in sequence after contacting the glass microspheres with the methyl silicone oil.

5. The process of claim 2, wherein the potassium dichromate-containing sulfuric acid solution is formulated as follows: adding 100-200g potassium dichromate into 1500mL water, heating to completely dissolve, and adding 500mL concentrated sulfuric acid; the petroleum ether solution containing the methyl silicone oil is prepared in the following way: 0.5-1mL of methyl silicone oil is added into 2L of petroleum ether.

6. The method according to claim 1 or 2, wherein the glass micro beads have a particle size of 70 to 110 μm.

7. Glass microspheres treated by the method of any one of claims 1 to 6.

8. A column agglutinating card comprising the glass microbead of claim 7.

9. Use of the glass beads according to claim 7 or the column agglutinating card according to claim 8 in an immunoassay.

10. The use of claim 9, wherein the immunoassay is a blood group test.

Technical Field

The present disclosure relates to a method of treating glass beads, and more particularly, to a method of enhancing hydrophobicity of glass beads to prevent generation of bubbles during packing of a microcolumn.

Background

The glass beads as a silicate material have the characteristics of uniform size, high roundness, high light transmittance, chemical corrosion resistance, impact resistance and the like. Glass beads have numerous applications in the biomedical field, including use in the preparation of microcolumns in Column Agglutination (CAT) assays or in the preparation of column agglutination cards having a plurality of microcolumns. The glass beads can be used for packing the microcolumn, and can be used as a separation medium in the microcolumn. However, for example, in the production process of a column aggregation card, a large number of fine glass beads are put in a narrow space, and thus air is immersed in a liquid reagent along with the putting of the glass beads, thereby generating fine bubbles. A certain amount of micro-bubbles remain in the packed column and may have a serious quality influence on the function of the product.

Generally, increasing the hydrophobicity of the glass surface improves its adsorption of air and other impurities. The prior treatment methods for preparing the super-hydrophobic glass mainly comprise the following methods.

1. The template method takes porous alumina, polystyrene and the like as templates, and generates the hydrophobic membrane with a special structure by special arrangement under the action of molecular action force of hydrogen bonds and ionic bonds. This method is not suitable for glass surfaces of minute volume and special shape.

2. The sol-gel method uses a high-activity compound as a precursor, forms transparent sol through hydrolysis and polycondensation reaction in a solution, and then coats the transparent sol on the surface of glass to form a hydrophobic film. Although the method has the characteristic of high light transmittance, the method is not suitable for the glass surface with tiny volume and special shape.

3. The vapor deposition method causes a certain physical change or chemical reaction of the substance in a gaseous state, and the reaction product is gradually condensed on the glass surface under the condition of continuous cooling, thereby forming a surface film. The method is widely used in various glass fields, and the prepared super-hydrophobic glass has good light transmittance and other properties, but needs large-scale high-cost equipment.

Therefore, no low-cost method suitable for the hydrophobic treatment of the surfaces of the glass beads is available at present.

Disclosure of Invention

In one aspect, provided herein is a method of treating glass microspheres comprising contacting the glass microspheres with potassium dichromate and methyl silicone oil in that order.

In some embodiments, the method comprises contacting the glass microspheres with the following solutions in sequence:

1) a sulfuric acid solution containing potassium dichromate; and

2) petroleum ether solution containing methyl silicone oil.

In some embodiments, the method comprises washing the glass beads with water and absolute ethanol in sequence after contacting the glass beads with the potassium dichromate, and then contacting the glass beads with the methyl silicone oil.

In some embodiments, the method comprises washing the glass microspheres with absolute ethanol and water sequentially after contacting the glass microspheres with the methyl silicone oil.

In some embodiments, the potassium dichromate-containing sulfuric acid solution is formulated as follows: 100 and 200g of potassium dichromate are added into 1500mL of water, and after the potassium dichromate is completely dissolved by heating, 500mL of concentrated sulfuric acid is added.

In some embodiments, the petroleum ether solution containing methyl silicone oil is formulated as follows: 0.5-1mL of methyl silicone oil is added into 2L of petroleum ether.

In some embodiments, the glass microspheres have a particle size of 70 to 110 μm.

In another aspect, provided herein are glass microspheres treated by the above-described method.

In another aspect, provided herein is a column aggregation card comprising the glass beads described above.

In another aspect, provided herein is the use of the glass bead or the column agglutinate card in an immunoassay.

In another aspect, the immunoassay is a blood group test.

The glass bead processing method provided by the invention is simple to operate and low in cost, the processed glass beads are good in light transmittance and strong in hydrophobic force, and the problem of generation of micro bubbles in the production process of the glass bead column agglutination card is solved.

Detailed Description

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art.

The glass is a common amorphous inorganic material, and is generally prepared by taking various inorganic minerals (such as quartz sand, borax, boric acid, barite, barium carbonate, limestone, feldspar, soda ash and the like) as main raw materials and adding a small amount of auxiliary raw materials. Its main components are silicon dioxide and other oxides (such as Na)₂O, CaO, etc.). In some embodiments, the glass employed herein is a silicate glass, such as soda lime glass, quartz glass, and the like. The "glass microspheres" are generally spherical and may have a particle size of between 1 μm and 1mm, for example, between 20 μm and 500 μm, or between 50 μm and 200 μm. Of course, the methods provided herein can also handle glass microspheres outside this size range.

The methyl silicone oil, also called methyl silicone oil and polydimethylsiloxane, is colorless transparent viscous liquid at normal temperature. It has excellent moisture resistance, light transmittance and chemical stability, and is widely used in various industrial fields such as electrical insulation, mold release, defoaming, damping, vibration prevention, rolling, dust prevention, water prevention, cosmetics, and the like. The methylsilicone oils used herein are generally not limited to a specific molecular weight range, but may be selected from those having a molecular weight between 6000-28000, corresponding to a viscosity of 100-1000mm²and/S. Preferably, the viscosity of the methyl silicone oil is 500 +/-25 mm²/S。

"Petroleum ether" is an organic solvent commonly used in laboratories, and the main components of the organic solvent comprise pentane, hexane and the like.

"blood typing" refers to the detection of antigens on blood cells and/or corresponding antibodies in plasma. Common blood group tests include the ABO and Rh detection of red blood cells and the detection of platelet and related antibodies.

The glass bead treatment method provided by the invention comprises the step of soaking the glass beads with a sulfuric acid solution of potassium dichromate and a petroleum ether solution of methyl silicone oil in sequence.

In addition, the method can also comprise the step of washing the glass beads by water and ethanol.

In addition, the method may further include drying the treated glass beads.

We have found that by increasing the hydrophobicity of the surface of the glass beads, the time of the glass bead soaking process in the production process of the glass bead column agglutination card can be prolonged, and the number of bubbles generated in the glass bead column agglutination production process can be greatly reduced.

Glass microspheres treated by the methods herein can be used in a variety of applications for detection by column agglutination. For example, it can be used for various blood type test cards, including the filling of positively-typed and negatively-typed microcolumns. After the glass beads are filled into the microcolumn, gaps among the glass beads play a role of a molecular sieve and are used for blocking agglutinated red blood cells. During the centrifugation of the blood type card, most of agglutinated red blood cells are positioned above the glass microsphere column, and unagglutinated red blood cells can reach the bottom of the microsphere column through pores among the glass microspheres under the action of centrifugal force. Therefore, the objective of distinguishing between positive and negative experimental results is achieved by separating agglutinated versus non-agglutinated erythrocytes.

The present invention will be described in further detail below with reference to specific examples.