阅读说明：本技术 一种银杏提取物脂质体及其制备方法 (Ginkgo extract liposome and preparation method thereof ) 是由 朱金花 徐静 张超 于 2019-02-21 设计创作，主要内容包括：本发明公开了一种银杏提取物脂质体及其制备方法,其要点为：将甘油10％～60％、胆固醇0.1％～0.5％、银杏提取物0.1％～4％、卵磷脂0.5％～5％、双丙甘醇1％～10％、角鲨烷1％～20％、其余为去离子水。按照工艺流程图的制作方法进行搅拌均匀形成初乳,通过高压均质机处理,高压均质压力为1000bar～1500bar,循环次数为2～5次,得到黄褐色银杏提取物脂质体。本发明的银杏提取物脂质体稳定性高、水分散性好、生物相容性好,可在常温条件下与化妆品配方任意互配；该制备方法简单可控,重复性好。(The invention discloses a ginkgo extract liposome and a preparation method thereof, and the key points are as follows: 10 to 60 percent of glycerin, 0.1 to 0.5 percent of cholesterol, 0.1 to 4 percent of ginkgo biloba extract, 0.5 to 5 percent of lecithin, 1 to 10 percent of dipropylene glycol, 1 to 20 percent of squalane and the balance of deionized water. Stirring uniformly according to the preparation method of the process flow chart to form primary emulsion, and treating by a high-pressure homogenizer at the high-pressure homogenizing pressure of 1000-1500bar and the circulation frequency of 2-5 times to obtain the fulvous ginkgo biloba extract liposome. The ginkgo biloba extract liposome has high stability, good water dispersibility and good biocompatibility, and can be freely matched with a cosmetic formula at normal temperature; the preparation method is simple and controllable, and has good repeatability.)

1. The ginkgo extract liposome is characterized by comprising the following components in percentage by mass:

2. the method for preparing the liposome of ginkgo extract according to claim 1, wherein: comprises the following steps of (a) carrying out,

(1) mixing and dissolving 10-60% of glycerol, 0.1-0.5% of cholesterol, 0.5-5% of lecithin and 1-10% of dipropylene glycol uniformly at 70-90 ℃ under the stirring condition of 200 rpm/min;

(2) adding 1-20% of squalane into the system obtained in the step (1) under the stirring conditions of 70-90 ℃ and 200rpm/min, and uniformly stirring to obtain a mixed phase 1;

(3) mixing and dissolving 0.1-4% of the ginkgo biloba extract and 1-10% of dipropylene glycol uniformly at 70-90 ℃ under the stirring condition of 200rpm/min, adding the mixture into the system obtained in the step (2), and uniformly stirring to obtain a mixed phase 2;

(4) heating water to 70 deg.C to obtain water phase;

(5) injecting the water phase into the mixed phase 2 in the step (3), stirring at 200rpm/min, and shearing at high speed of 5000rpm/min to obtain semi-permeable viscous gingko extract liposome colostrum;

(6) cooling the gingko extract liposome primary emulsion obtained in the step (5) to-20 ℃, keeping the temperature for 24 hours, then heating to 45-55 ℃, and shearing at 5000rpm/min for 2 min;

(7) and (3) treating the system obtained in the step (6) by using a high-pressure homogenizer, wherein the homogenizing pressure is 1000-1500bar, the cycle time is 2-5 times, and the discharging temperature is controlled as follows: and (4) obtaining the ginkgo biloba extract liposome with yellow brown appearance at 15-25 ℃.

3. The method for preparing the liposome of ginkgo biloba extract according to claim 2, wherein the method comprises the steps of: the ginkgo biloba extract liposome is used in the preparation of cosmetics.

The technical field is as follows:

the invention relates to the technical field of carrier systems in the technology of cosmetic preparation, in particular to ginkgo biloba extract liposome and a preparation method thereof.

Background art:

squalane has an alkane structure, good ductility, smooth skin feel, no greasiness and no heaviness. Has mild property, no sensitization, and no acne or pox. Moderate moistening degree, most of the skin can be used. The skin care cream has the advantages that the skin care cream has the components in the skin, has particularly good skin-friendly property, can be highly compatible with sebum secreted by the skin, forms a breathable protective film on the surface layer of the skin and plays the effects of moisturizing and moistening the skin. Can effectively relieve dry skin and maintain the skin elastic and full.

Ginkgo biloba is the oldest middle-aged plant, has been used as a traditional Chinese medicine for a long time, and the extract thereof has attracted international high attention in recent years. The chemical components of the ginkgo leaf are complex, so far, more than 170 compounds are found, and the main active components in the ginkgo leaf extract are flavonoids and terpene lactones, so that the ginkgo leaf has the pharmacological action of both flavonoids and terpene lactones, and the popularization and the application of the medicinal value of the ginkgo leaf extract are very important. Its main effects in cosmetics include: the anti-inflammatory effect is as follows: kim SJ reports that the main components (tetrahydroxyflavone and ginkgolide) of the ginkgo extract have obvious anti-inflammatory effect, and the effect of the ginkgo extract on human skin fibroblasts is researched by MIT and cytometry, so that the ginkgo extract can promote the generation of collagen and extracellular fiber connectors, and particularly has more obvious flavonoid effect; ② the free radical removal and antioxidation: the current research shows that the main component flavonoid compounds of the gingko extract related to the toxicity of local oxygen free radicals generated during the occurrence and development of skin cuticle sclerosis and anoxia aging belong to natural antioxidants, and can clear hydroxyl free radicals, lipid peroxidation free radicals, superoxide anions and the like, so that the oxygen free radical reaction and the lipid peroxidation reaction are inhibited; meanwhile, the ginkgo biloba extract can also be used for regulating and improving the activity of antioxidant enzymes such as superoxide dismutase, glutathione peroxidase and the like, thereby accelerating the elimination of free radicals and slowing down the peroxidation damage of oxygen radicals and lipid.

The Ginkgo biloba extract has antiinflammatory, antioxidant, and antiaging effects. However, ginkgo biloba extracts have the disadvantages of poor water solubility, low bioavailability, strong characteristic odor, and the like, which causes certain problems in the use of cosmetics. To solve these problems, we have used nanostructured liposomes to encapsulate ginkgo biloba extracts.

Liposome technology was the first nanoliposome carrier system to be applied in the cosmetic field. Due to its special phospholipid bilayer structure, it can carry various hydrophilic, hydrophobic or amphiphilic substances, which are encapsulated in the internal aqueous phase of the liposome, or inserted into the lipid bilayer, or adsorbed and linked on the surface of the liposome. The liposome has special hydrophilic vesicle structure, and can improve the stability of the coated component, promote the transdermal absorption of the active component, prolong the action time, target the local site, reduce the toxic and side effects, etc.

Common preparation methods of the liposome comprise an ethanol injection method, a vortex dispersion method, an ultrasonic dispersion method and a reverse phase evaporation method, the methods usually involve the addition and evaporation processes of an organic solvent, the process is complex, the particle size distribution of the obtained liposome is not uniform, the stability is general, the entrapment rate of an active substance is not high, and the problem of drug leakage exists.

In the project, the problems are focused, and the ginkgo biloba extract is wrapped by a high-pressure homogenization method, which comprises the key steps of: firstly, after the high-temperature preparation of the liposome colostrum is finished, the temperature is reduced to-20 ℃ for storage for 24 hours, then the temperature is raised to 45-55 ℃, in the process, the concentration of active substances around the phospholipid is higher, the active substances can be more fully wrapped in the liposome after temperature rise and phase inversion, and the encapsulation efficiency is improved; secondly, homogenizing the liquid by using a high-pressure homogenizing device, controlling the discharging temperature to be 15-25 ℃, keeping the particle size of the system in a lower range by using the lower discharging temperature, and simultaneously effectively preventing the leakage of active matters.

Therefore, the complex method is used for wrapping the ginkgo biloba extract, so that the problem of traditional preparation of the liposome can be well solved, the entrapment rate and stability can be improved, the bioavailability of the ginkgo biloba extract is improved, and the absorption of a human body is promoted. The invention is suitable for safe and continuous production, can solve the problems of stability, encapsulation efficiency, slow release effect, convenient addition to water and the like of the ginkgo extract, and can be widely applied to the preparation of various cosmetics.

The invention content is as follows:

the invention aims to: provides a preparation method of liposome with high loading capacity, good water solubility, good stability and excellent compatibility, and solves the problems of poor water solubility, low bioavailability, strong characteristic smell and the like of the ginkgo biloba extract in the prior art. Meanwhile, squalane is used as oil, which is a component existing in the skin, has good permeability and good skin-friendly property, can be highly compatible with sebum secreted by the skin, forms a breathable protective film on the surface layer of the skin, and plays a role in moisturizing and moistening the skin. Can effectively relieve dry skin and maintain the skin elastic and full.

The invention aims to provide a ginkgo biloba extract liposome for a skin care product, and solves the problems that a ginkgo biloba extract is stable in the cosmetic and is effectively absorbed by the skin.

The invention also aims to provide a preparation method of the ginkgo biloba extract liposome for the skin care product.

The invention provides a ginkgo extract liposome with high stability and good water dispersibility and a preparation method thereof, and the ginkgo extract liposome is characterized by comprising the following components in percentage by mass:

a preparation method of semen Ginkgo extract liposome with high stability and good water dispersibility comprises the following steps,

(1) mixing and dissolving 10-60% of glycerol, 0.1-0.5% of cholesterol, 0.5-5% of lecithin and 1-10% of dipropylene glycol uniformly at 70-90 ℃ under the stirring condition of 200 rpm/min;

(2) adding 1-20% of squalane into the system obtained in the step (1) under the stirring conditions of 70-90 ℃ and 200rpm/min, and uniformly stirring to obtain a mixed phase 1;

(3) mixing and dissolving 0.1-4% of the ginkgo biloba extract and 1-10% of dipropylene glycol uniformly at 70-90 ℃ under the stirring condition of 200rpm/min, adding the mixture into the system obtained in the step (2), and uniformly stirring to obtain a mixed phase 2;

(4) heating water to 70 deg.C to obtain water phase;

(5) injecting the water phase into the mixed phase 2 in the step (3), stirring at 200rpm/min, and shearing at high speed of 5000rpm/min to obtain semi-permeable viscous gingko extract liposome colostrum;

(6) cooling the gingko extract liposome primary emulsion obtained in the step (5) to-20 ℃, keeping the temperature for 24 hours, then heating to 45-55 ℃, and shearing at 5000rpm/min for 2 min;

(7) and (3) treating the system obtained in the step (6) by using a high-pressure homogenizer, wherein the homogenizing pressure is 1000-1500bar, the cycle time is 2-5 times, and the discharging temperature is controlled as follows: and (4) obtaining the ginkgo biloba extract liposome with yellow brown appearance at 15-25 ℃.

As a further step of the technical scheme, the average particle size of the ginkgo biloba extract liposome prepared by the method is 100-500 nm.

The ginkgo biloba extract is wrapped by the liposome, so that the water solubility of the ginkgo biloba extract is improved, and the photo-thermal stability of the ginkgo biloba extract is improved. In addition, the invention does not contain organic solvent, thereby avoiding the damage to the skin.

Compared with the scheme in the prior art, the invention has the advantages that:

(1) the preparation of the ginkgo biloba extract liposome of the invention is controllable. By adjusting the formulation of liposome, the high pressure homogenization circulation frequency and temperature, etc., 0.1-4% of ginkgo biloba extract liposome can be prepared.

(2) The invention improves the loading capacity of the ginkgo biloba extract, and can simultaneously avoid leakage caused by the extrusion of active substances in the storage process; after the active component is embedded in the liposome micropore structure, the chemical degradation performance of the active component can be reduced, so that the stability of the active component is enhanced.

(3) The liposome technology of the invention improves the stability of the active substance ginkgo extract and promotes the generation of collagen and extracellular fibrous connectors; improving the capability of removing hydroxyl free radicals, lipid peroxidation free radicals, superoxide anions and the like, thereby inhibiting the effects of oxygen free radical reaction and lipid peroxidation; is favorable for fully exerting the efficacy of anti-inflammation, anti-oxidation, anti-aging and the like. The ginkgo biloba extract liposome prepared by the invention has good stability, good water solubility and excellent compatibility.

(4) The ginkgo biloba extract liposome prepared by the invention is less than 400nm, has good permeability by utilizing squalane, can be highly compatible with sebum secreted by skin, forms a layer of breathable protective film on the surface layer of the skin, and plays the effects of moisturizing and moistening the skin. Thus, a good closed system can be formed on the skin, the hydration of the skin can be enhanced, and the moisturizing performance of the product can be improved when the skin care product is applied.

(5) The ginkgo biloba extract liposome prepared by the invention has good affinity with the stratum corneum of the skin, and can effectively convey and permeate the active components into the stratum corneum, so that the active components effectively act on deep cells of the skin, and the deep cells effectively exert the efficacies of anti-inflammation, antioxidation, anti-aging and the like.

(6) The preparation method is simple and has good repeatability. It can be added into cosmetic preparation, or used directly.

Drawings

The invention is further described with reference to the following figures and examples:

FIG. 1 is a particle size distribution of liposomes of Ginkgo biloba extract prepared in example 1;

FIG. 2 is a flow chart of a method for preparing liposomes of Ginkgo biloba extract prepared in example 1;

FIG. 3 shows the variation of mean particle size and pdi for different dilution factors for the liposomes of Ginkgo biloba extract prepared in example 1;

FIG. 4 is a graph showing the particle size change of the liposomes of Ginkgo biloba extract prepared in example 1 under different storage conditions (25 deg.C, 40 deg.C and light irradiation)

The specific implementation mode is as follows:

the above-described scheme is further illustrated below with reference to specific examples. The following description is only a preferred embodiment for embodying the object, effect and technical features of the present invention, and does not limit the scope of the present invention.