阅读说明：本技术 一种细胞因子免疫佐剂制备用细胞破碎机 (Cell crusher for preparing cytokine immunologic adjuvant ) 是由 陈龙刚 于 2020-04-29 设计创作，主要内容包括：本发明提出了一种细胞因子免疫佐剂制备用细胞破碎机,包括底座、筒体和破碎机构；破碎机构包括转轴、多个弧形板和第一电机；转轴水平设置在筒体内部且其两端均与筒体转动连接,多个弧形板均沿转轴轴向延伸且多个弧形板围绕转轴呈圆周分布,弧形板两端均通过连接杆与转轴连接,弧形板的弧形开口朝向转轴,弧形板外侧面分布有多个第一破碎杆,第一破碎杆远离弧形板一端设有第一破碎刀,弧形板内侧面分布有多个第二破碎杆,第二破碎杆远离弧形板一端设有第二破碎刀。本发明破碎速度快,破碎效率高,保证细胞分离出的蛋白质的活性；通过设置第一驱动机构与第二驱动机构相互配合共同驱动筒体上下往复移动,提高破碎效果。(The invention provides a cell crusher for preparing a cytokine immunologic adjuvant, which comprises a base, a cylinder and a crushing mechanism, wherein the base is provided with a groove; the crushing mechanism comprises a rotating shaft, a plurality of arc-shaped plates and a first motor; the pivot level sets up inside the barrel and its both ends all are connected with the barrel rotation, a plurality of arcs all are the circumference along pivot axial extension and a plurality of arcs around the pivot and distribute, the arc both ends all are connected with the pivot through the connecting rod, the arc opening of arc is towards the pivot, the arc lateral surface distributes and has a plurality of first broken poles, first broken pole is kept away from arc one end and is equipped with first broken sword, the arc medial surface distributes and has a plurality of broken poles of second, the broken pole of second is kept away from arc one end and is equipped with the broken sword of second. The invention has high crushing speed and high crushing efficiency, and ensures the activity of protein separated from cells; through setting up first actuating mechanism and the mutual cooperation of second actuating mechanism and drive barrel reciprocating motion from top to bottom jointly, improve crushing effect.)

1. A cell crusher for preparing a cytokine immunologic adjuvant is characterized by comprising a base, a cylinder and a crushing mechanism;

the base is horizontally arranged, a first support and a second support which are vertically and oppositely arranged are arranged on the base, the barrel is positioned above the base, the central axis of the barrel is parallel to the base, and two ends of the barrel are respectively connected with the first support and the second support;

the crushing mechanism comprises a rotating shaft, a plurality of arc-shaped plates and a first motor; the rotating shaft is horizontally arranged in the cylinder body, two ends of the rotating shaft are rotatably connected with the cylinder body, and the rotating shaft is in transmission connection with the first motor and is driven by the first motor to rotate; a plurality of arcs all are along pivot axial extension and a plurality of arcs are the circumference around the pivot and distribute, and the arc both ends all are connected with the pivot through the connecting rod, and the arc opening of arc is towards the pivot, and the arc lateral surface distributes and has a plurality of first broken poles, and first broken pole is kept away from arc one end and is equipped with first broken sword, and the arc medial surface distributes and has a plurality of broken poles of second, and the broken pole of second is kept away from arc one end and is equipped with the broken sword of second.

2. The cell crusher for preparing a cytokine immunoadjuvant according to claim 1, wherein the second crushing rod has a plurality of second crushing balls spaced apart from each other, and a plurality of third crushing balls are distributed on the inner wall of the cylinder.

3. The cell crusher for preparing the cytokine immunoadjuvant according to claim 1, wherein the first support is provided with a first driving mechanism connected with the cylinder, the second support is provided with a second driving mechanism connected with the cylinder, and the first driving mechanism and the second driving mechanism are mutually matched to drive the cylinder to reciprocate up and down.

4. The cell crusher for cytokine immunoadjuvant preparation according to claim 3, wherein the first driving mechanism comprises a first mounting plate, a first support shaft, a first turntable, and a second motor; the first mounting disc is fixedly mounted on the first support, a first sliding groove is vertically arranged on the first mounting disc, and a first movable sleeve capable of moving up and down in the first sliding groove is connected in the first sliding groove in a sliding fit manner; one end of the first supporting shaft is connected with the first movable sleeve, and the other end of the first supporting shaft is connected with the cylinder; first carousel rotates and installs on first mounting disc and rotates by the drive of second motor, and first carousel periphery has arranged a plurality of first poker bars that can stir first movable sleeve and remove.

5. The cell crusher for cytokine immunoadjuvant preparation according to claim 3, wherein the second driving mechanism comprises a second mounting plate, a second support shaft, a second turntable, and a third motor; the second mounting disc is fixedly mounted on the second support, a second sliding groove is vertically arranged on the second mounting disc, and a second movable sleeve capable of moving up and down in the second sliding groove is connected in the second sliding groove in a sliding fit manner; one end of the second support shaft is connected with the second movable sleeve, and the other end of the second support shaft is connected with the cylinder body; the second turntable is rotatably installed on the second installation disc and is driven by the third motor to rotate, and a plurality of second poking rods capable of poking the second movable sleeve to move are arranged on the periphery of the second turntable in the circumferential direction.

6. The cell disruptor for cytokine immunoadjuvant preparation according to any one of claims 3 to 5, the buffer device is characterized by further comprising a buffer mechanism connected with the barrel, the buffer mechanism comprises a first buffer plate and a second buffer plate, the first buffer plate is connected with the bottom end of the barrel, the second buffer plate is located below the first buffer plate, two ends of the second buffer plate are respectively connected with the first support, the second support is connected with the second support, a plurality of buffer rods located around the barrel are arranged on the second annular plate, the top end of each buffer rod penetrates through the first buffer plate, the buffer rods are in sliding fit with the first buffer plate, a limiting plate is arranged at the top end of each buffer rod, a first buffer spring and a second buffer spring are sleeved on each buffer rod, the bottom end of each first buffer spring is connected with the second buffer plate, the top end of each first buffer spring is connected with the bottom end of the first buffer plate, and the top end of each second buffer spring is connected with.

7. The cell crusher for preparing the cytokine immunoadjuvant according to any one of claims 1 to 3, wherein the top end of the cylinder is provided with a feed inlet and the bottom end thereof is provided with a discharge outlet, and valves are respectively connected in the feed inlet and the discharge outlet in a matching manner.

Technical Field

The invention relates to the technical field of cell disruption, in particular to a cell disruption machine for preparing a cytokine immunologic adjuvant.

Background

The cell disruption technology is a technology which utilizes external force to destroy cell membranes and cell walls so as to release cell contents including target product components. However, in the prior art, the cell disruption device has a complex structure and low disruption efficiency, so that a rapid and effective cell disruption effect cannot be realized, and the disruption time is long, so that the activity of the protein separated after cell disruption is easily lost.

Disclosure of Invention

Based on the technical problems in the background art, the invention provides a cell crusher for preparing a cytokine immunologic adjuvant.

The invention provides a cell crusher for preparing a cytokine immunologic adjuvant, which comprises a base, a cylinder and a crushing mechanism, wherein the base is provided with a groove;

the base is horizontally arranged, a first support and a second support which are vertically and oppositely arranged are arranged on the base, the barrel is positioned above the base, the central axis of the barrel is parallel to the base, and two ends of the barrel are respectively connected with the first support and the second support;

the crushing mechanism comprises a rotating shaft, a plurality of arc-shaped plates and a first motor; the rotating shaft is horizontally arranged in the cylinder body, two ends of the rotating shaft are rotatably connected with the cylinder body, and the rotating shaft is in transmission connection with the first motor and is driven by the first motor to rotate; a plurality of arcs all are along pivot axial extension and a plurality of arcs are the circumference around the pivot and distribute, and the arc both ends all are connected with the pivot through the connecting rod, and the arc opening of arc is towards the pivot, and the arc lateral surface distributes and has a plurality of first broken poles, and first broken pole is kept away from arc one end and is equipped with first broken sword, and the arc medial surface distributes and has a plurality of broken poles of second, and the broken pole of second is kept away from arc one end and is equipped with the broken sword of second.

Preferably, a plurality of first crushing balls are arranged on the first crushing rod at intervals, a plurality of second crushing balls are arranged on the second crushing rod at intervals, and a plurality of third crushing balls are distributed on the inner wall of the cylinder body.

Preferably, a first driving mechanism connected with the barrel is installed on the first support, a second driving mechanism connected with the barrel is installed on the second support, and the first driving mechanism and the second driving mechanism are matched with each other to drive the barrel to reciprocate up and down.

Preferably, the first driving mechanism comprises a first mounting disc, a first supporting shaft, a first rotating disc and a second motor; the first mounting disc is fixedly mounted on the first support, a first sliding groove is vertically arranged on the first mounting disc, and a first movable sleeve capable of moving up and down in the first sliding groove is connected in the first sliding groove in a sliding fit manner; one end of the first supporting shaft is connected with the first movable sleeve, and the other end of the first supporting shaft is connected with the cylinder; first carousel rotates and installs on first mounting disc and rotates by the drive of second motor, and first carousel periphery has arranged a plurality of first poker bars that can stir first movable sleeve and remove.

Preferably, the second driving mechanism comprises a second mounting plate, a second supporting shaft, a second turntable and a third motor; the second mounting disc is fixedly mounted on the second support, a second sliding groove is vertically arranged on the second mounting disc, and a second movable sleeve capable of moving up and down in the second sliding groove is connected in the second sliding groove in a sliding fit manner; one end of the second support shaft is connected with the second movable sleeve, and the other end of the second support shaft is connected with the cylinder body; the second turntable is rotatably installed on the second installation disc and is driven by the third motor to rotate, and a plurality of second poking rods capable of poking the second movable sleeve to move are arranged on the periphery of the second turntable in the circumferential direction.

Preferably, still include the buffer gear who is connected with the barrel, buffer gear includes first buffer board and second buffer board, first buffer board is connected with the barrel bottom, the second buffer board is located first buffer board below and its both ends respectively with first support, the second leg joint, be equipped with a plurality of buffer rods that are located barrel all around on the second annular plate, first buffer board is passed on the buffer rod top and buffer rod and first buffer board sliding fit, the buffer rod top is equipped with the limiting plate, the cover has first buffer spring and second buffer spring on the buffer rod, first buffer spring bottom is connected and the top is connected with first buffer board bottom with the second buffer board, second buffer spring top is connected and its top and limiting plate connection with first buffer board top.

Preferably, the top end of the cylinder body is provided with a feed inlet, the bottom end of the cylinder body is provided with a discharge outlet, and valves are matched and connected in the feed inlet and the discharge outlet.

The cell crusher for preparing the cytokine immunologic adjuvant provided by the invention has the advantages that raw materials are added into the cylinder body from the feeding hole, the first motor is started, the first motor drives the rotating shaft to rotate, the rotating shaft drives the plurality of arc-shaped plates to rotate, the arc-shaped plates, the first crushing rod and the second crushing rod stir the raw materials, and cells are contacted and collided with the first crushing cutter, the second crushing cutter, the first crushing ball, the second crushing ball and the third crushing ball to be crushed; reciprocating motion about the common drive barrel of mutually supporting through setting up first actuating mechanism and second actuating mechanism for raw materials in the barrel rocks, improves the collision efficiency of cell and first broken sword, the broken sword of second, first broken ball, the broken ball of second, the broken ball of third, improves crushing effect.

Drawings

FIG. 1 is a schematic structural diagram of a cell crusher for preparing a cytokine immunoadjuvant according to the present invention;

FIG. 2 is a schematic structural diagram of a first driving mechanism of a cell crusher for preparing a cytokine immunoadjuvant according to the present invention;

FIG. 3 is a schematic structural diagram of a second driving mechanism of a cell crusher for preparing a cytokine immunoadjuvant according to the present invention.

Detailed Description

Referring to fig. 1-3, the invention provides a cell crusher for preparing a cytokine immunoadjuvant, comprising a base 1, a cylinder 2 and a crushing mechanism; wherein:

the base 1 is horizontally arranged, and the base 1 is provided with a first support 3 and a second support 4 which are vertically and oppositely arranged.

The barrel 2 is located base 1 top and its central axis is on a parallel with base 1, and barrel 2 both ends are connected with first support 3, second support 4 respectively, and 2 inner walls of barrel distribute have a plurality of broken balls 14 of third, and barrel 2 top is equipped with feed inlet 33 and its bottom is equipped with discharge gate 34, all cooperatees in feed inlet 33, the discharge gate 34 and is connected with the valve.

The crushing mechanism comprises a rotating shaft 5, a plurality of arc-shaped plates 6 and a first motor 7. The 5 level settings of pivot are all rotated with barrel 2 at barrel 2 inside and its both ends and are connected, and pivot 5 is connected and is rotated by first motor 7 drive with first motor 7 transmission. A plurality of arcs 6 all are along 5 axial extensions of pivot and a plurality of arcs 6 are the circumference around pivot 5 and distribute, and 6 both ends of arc all are connected with pivot 5 through the connecting rod, and the arc opening of arc 6 is towards pivot 5. A plurality of first crushing rods 8 are distributed on the outer side surface of the arc-shaped plate 6, a plurality of first crushing balls 12 are arranged on the first crushing rods 8 at intervals, and a first crushing knife 9 is arranged at one end, far away from the arc-shaped plate 6, of each first crushing rod 8. A plurality of second crushing rods 10 are distributed on the inner side surface of the arc-shaped plate 6, a plurality of second crushing balls 13 are arranged on the second crushing rods 10 at intervals, and a second crushing knife 11 is arranged at one end, far away from the arc-shaped plate 6, of each second crushing rod 10.

According to the invention, raw materials are added into the barrel body from the feeding hole, the first motor is started, the first motor drives the rotating shaft to rotate, the rotating shaft drives the plurality of arc-shaped plates to rotate, the arc-shaped plates, the first crushing rod and the second crushing rod stir the raw materials, and cells are contacted and collided with the first crushing cutter, the second crushing cutter, the first crushing ball, the second crushing ball and the third crushing ball to be crushed.

In a specific embodiment, a first driving mechanism connected with the barrel 2 is installed on the first support 3, a second driving mechanism connected with the barrel 2 is installed on the second support 4, the first driving mechanism and the second driving mechanism are matched with each other to drive the barrel 2 to reciprocate up and down, so that raw materials in the barrel are shaken, the collision efficiency of cells with the first crushing cutter, the second crushing cutter, the first crushing ball, the second crushing ball and the third crushing ball is improved, and the crushing effect is improved.

Specifically, the first drive mechanism includes a first mounting plate 15, a first support shaft 16, a first rotating plate 17, and a second motor. First mounting disc 15 is fixed mounting on first support 3, is equipped with vertical first spout 18 of arranging on first mounting disc 15, and sliding fit connects with the first movable sleeve 19 that can reciprocate in first spout 18. One end of the first supporting shaft 16 is connected to the first movable sleeve 19 and the other end thereof is connected to the cylinder 2. The first rotating disc 17 is rotatably mounted on the first mounting disc 15 and is driven by the second motor to rotate, and a plurality of first poking rods 20 capable of poking the first movable sleeve 19 to move are circumferentially arranged on the outer periphery of the first rotating disc 17. The second motor drives the first rotating disk 15 to rotate continuously, the first poking rod 20 on the first rotating disk 17 intermittently pokes the first movable sleeve 19 to move upwards in the first sliding groove 18, the first movable sleeve 19 drives the cylinder 1 to move upwards through the first supporting shaft 16, and when the first poking rod 20 is separated from the first movable sleeve 19, the first movable sleeve 19 drives the cylinder 1 to move downwards automatically under the action of gravity.

Further, the second driving mechanism includes a second mounting plate 21, a second supporting shaft 22, a second turntable 23, and a third motor. The second mounting disc 21 is fixedly mounted on the second support 4, a second sliding groove 24 which is vertically arranged is arranged on the second mounting disc 21, and a second movable sleeve 25 which can move up and down in the second sliding groove 24 is connected in the second sliding groove 24 in a sliding fit manner. One end of the second support shaft 22 is connected with the second movable sleeve 25 and the other end thereof is connected with the cylinder 2. The second rotating disc 23 is rotatably mounted on the second mounting disc 21 and is driven by a third motor to rotate, and a plurality of second poking rods 26 capable of poking the second movable sleeve 25 to move are circumferentially arranged on the outer periphery of the second rotating disc 23. The third motor drives the second rotary table 21 to continuously rotate, the second poking rod 26 on the second rotary table 23 intermittently pokes the second movable sleeve 25 to move upwards in the second sliding groove 24, the second movable sleeve 25 drives the cylinder body 1 to move upwards through the second supporting shaft 22, and when the second poking rod 26 is separated from the second movable sleeve 25, the second movable sleeve 25 drives the cylinder body 1 to automatically move downwards under the action of gravity.

In the specific embodiment, still include the buffer gear who is connected with barrel 2, buffer gear includes first buffer board 27 and second buffer board 28, first buffer board 27 is connected with barrel 2 bottom, second buffer board 28 is located first buffer board 27 below and its both ends are connected with first support 3, second support 4 respectively, be equipped with a plurality of buffer rods 29 that are located barrel 2 periphery on the second annular plate, first buffer board 27 is passed and buffer rods 29 and first buffer board 27 sliding fit are passed to buffer rods 29 top, buffer rods 29 top is equipped with limiting plate 30, the cover has first buffer spring 31 and second buffer spring 32 on the buffer rods 29, first buffer spring 31 bottom is connected with second buffer board 28 and the top is connected with first buffer board 27 bottom, second buffer spring 32 top is connected with first buffer board 27 top and its top is connected with limiting plate 30. Through setting up buffer gear, slow down the speed that barrel 1 reciprocated, improve the stability of breaker.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.