阅读说明：本技术 地榆炭在体股癣、手足癣防治中的应用 (Application of garden burnet charcoal in preventing and treating tinea corporis, tinea cruris, tinea manus and tinea pedis ) 是由 顾琦 梁莹 陈军 顾薇 苏曼 王昊 于 2020-06-21 设计创作，主要内容包括：本发明提供地榆炭在体股癣、手足癣防治中的应用,及其在药物或日用品制备中应用。同时提供没食子酸、焦性没食子酸、没食子酸甲酯、没食子酸乙酯、原儿茶醛中一个或多个成分在体股癣、手足癣防治中的应用,及其在药物或日用品制备中应用。红色毛癣菌是体股癣和手足癣的主要致病菌,本发明表明地榆炭能显著抑制其生长,效果与皮肤癣常用中药苦参、土荆皮相当。给药地榆炭后,目镜观察红色毛癣菌生长受到显著抑制。临床疗效观察表明,用含量0.5％地榆炭凝胶治疗4周后患者显愈率达96.15％,其中一半患者痊愈。同时,地榆中主要成分没食子酸、焦性没食子酸、没食子酸甲酯、没食子酸乙酯、原儿茶醛均具显著抗红色毛癣菌功效,以上内容未见报道。(The invention provides application of garden burnet charcoal in preventing and treating tinea corporis, tinea cruris, tinea manus and tinea pedis, and application of garden burnet charcoal in preparation of medicines or daily necessities. Simultaneously provides the application of one or more components of gallic acid, pyrogallic acid, gallic acid methyl ester, gallic acid ethyl ester and protocatechuic aldehyde in preventing and treating tinea corporis and cruris, tinea manus and pedis, and the application in the preparation of medicines or daily necessities. The present invention shows that the sanguisorba root charcoal can obviously inhibit the growth of tinea corporis, tinea cruris, tinea manus and tinea pedis, and the effect of the sanguisorba root charcoal is equivalent to that of common traditional Chinese medicines of radix sophorae flavescentis and golden larch bark for treating tinea. After the sanguisorba officinalis charcoal is administrated, the growth of trichophyton rubrum is obviously inhibited by ocular observation. Clinical curative effect observation shows that the cure rate of patients after 4 weeks of treatment by 0.5 percent sanguisorba officinalis charcoal gel reaches 96.15 percent, and half of patients are cured. Meanwhile, the main components of the garden burnet, namely gallic acid, pyrogallic acid, gallic acid methyl ester, gallic acid ethyl ester and protocatechuic aldehyde, have remarkable anti-trichophyton rubrum effects, and the contents are not reported.)

1. The sanguisorba officinalis charcoal is applied to prevention and treatment of tinea corporis, tinea cruris, tinea manus and tinea pedis.

2. The garden burnet root charcoal is applied to the preparation of medicaments or daily necessities for preventing and treating tinea corporis, tinea cruris, tinea manus and pedis.

3. A medicine or daily necessities capable of preventing and treating tinea corporis, tinea cruris, tinea manus and tinea pedis is characterized in that: the prescription contains charred radix Sangusorbae.

4. The medicament or daily item according to claim 3, wherein: the radix Sangusorbae charcoal is radix Sangusorbae charcoal powder or extract.

5. The medicament or daily item according to claim 4, wherein: the extraction solvent of the garden burnet root charcoal extract is ethanol water solution.

6. The medicament or daily item according to claim 5, wherein: the extraction solvent of the garden burnet root charcoal extract is a 75% ethanol extract.

7. Application of one or more of gallic acid, pyrogallic acid, gallic acid methyl ester, gallic acid ethyl ester, and protocatechuic aldehyde in preventing and treating tinea corporis, tinea cruris, tinea manus, and tinea pedis.

8. A medicine or daily necessities capable of preventing and treating tinea corporis, tinea cruris, tinea manus and tinea pedis is characterized in that: the prescription contains one or more of gallic acid, pyrogallic acid, gallic acid methyl ester, gallic acid ethyl ester, and protocatechuic aldehyde.

Technical Field

The invention belongs to the field of external application of traditional Chinese medicines, and particularly relates to application of carbonized sanguisorba root in prevention and treatment of tinea corporis, tinea cruris, tinea manus and tinea pedis.

Background

The pathogenic fungi of tinea corporis, cruris, tinea manuum and tinea pedis are dermatophytes which are both characterized by being cutiphilic. About more than 40 kinds of dermatophytes are available, and about more than 20 kinds of dermatophytes can cause diseases in human beings or animals. The domestic circulation and regulation data of various regions and different hospitals show that pathogenic bacteria of tinea corporis, tinea cruris, tinea manus and pedis are approximately the same, the most common is trichophyton rubrum which is the main pathogenic bacteria, and the other pathogenic bacteria comprise trichophyton mentagrophytes, microsporum canis, microsporum gypseum and the like. Dermatophytes can be transmitted from person to person, animal to person, pollutant to person, and various parts of the human body. The physique and environmental factors of patients play a certain role in the pathogenesis, such as the damp-heat area and the high-temperature season are the causes of tinea corporis, tinea cruris, tinea manuum and tinea pedis, the patients wear airtight and over-tight clothes, and the obese and hyperhidrosis patients or drivers are prone to suffer from femoral diseases; the feed is used for raising pets, diabetics, close contact infected persons and pollutants, and patients suffering from tinea manus, tinea pedis or onychomycosis are infected by other fungal infections at other parts and are susceptible to tinea corporis.

The clinical treatment of tinea corporis, cruris, tinea manus and pedis generally adopts local external administration, however, patients who apply glucocorticoid and immunosuppressant locally or systemically for a long time can make skin lesions atypical and form 'indiscernible tinea'. ("Chinese guide for diagnosis and treatment of tinea corporis and tinea cruris" 2018 revised edition)

The radix Sangusorbae is dried root of Sanguisorbae officinalis L. or Sanguisorbae officinalis L. var. longifolia (Bert.) Kuet Li, which is commonly called "sanguisorbae officinalis". The charred radix Sangusorbae is processed by charring method, and has brown surface, brown interior, charred fragrance, and bitter taste. Sanguisorba root, charred and slightly cold in nature, bitter, sour and astringent in flavor, enters liver and large intestine meridians. Has effects in cooling blood, stopping bleeding, removing toxic substance, and healing sore, and can be used for treating hematochezia, hemorrhoidal bleeding, bloody dysentery, metrorrhagia, scald due to hot water and fire, carbuncle, swelling, and sore. The current drug effect reports of the sanguisorba officinalis charcoal are mainly for hemostasis and burn and scald treatment (in the 'Chinese pharmacopoeia' 2015 edition), the report on the antibacterial effect is few, the report on the tinea corporis and cruris pathogenic bacteria such as trichophyton rubrum and the like is not found, the report on the prevention or treatment of the tinea corporis and cruris is not found, and the description of the type is not found in ancient books.

Disclosure of Invention

The invention provides application of garden burnet root charcoal in preventing and treating tinea corporis, tinea cruris, tinea manus and tinea pedis.

Simultaneously provides the application of the garden burnet root charcoal in preparing the medicaments or daily necessities for preventing and treating tinea corporis, tinea cruris, tinea manus and pedis.

The radix Sangusorbae charcoal is radix Sangusorbae charcoal powder or extract.

As a further improvement, the extraction solvent of the garden burnet root charcoal extract is an ethanol water solution.

As a further improvement, the extraction solvent of the garden burnet root charcoal extract is 75% ethanol extract. The invention also provides application of one or more components of gallic acid, pyrogallic acid, gallic acid methyl ester, gallic acid ethyl ester and protocatechuic aldehyde in preventing and treating tinea corporis, tinea cruris and tinea manus and pedis.

And one or more components of gallic acid, pyrogallic acid, gallic acid methyl ester, gallic acid ethyl ester and protocatechuic aldehyde in the preparation of medicines or daily necessities for preventing and treating tinea corporis, tinea cruris, tinea manus and pedis.

Advantageous effects

The invention shows that the sanguisorba officinalis charcoal extract can obviously inhibit the growth of trichophyton rubrum (ATCC MYA-4438), the minimum inhibitory concentration MIC of the sanguisorba officinalis charcoal extract reaches 64 mu g/ml, the effect of the sanguisorba officinalis charcoal extract is equivalent to that of common traditional Chinese medicines of radix sophorae flavescentis and golden larch bark for tinea corporis and tinea cruris and tinea manus and pedis, the sanguisorba officinalis charcoal extract is superior to that of crude medicinal materials of rheum officinale, clove, common cnidium fruit, radix stemonae, scutellaria baicalensis, fructus kochiae, male. The flora shape of the sanguisorba officinalis after administration is observed by 10 times of an ocular lens and 40 times of an objective lens, hypha separation becomes fuzzy after administration of sanguisorba officinalis charcoal (0.5 × MIC), the medicine influences the growth of hypha, and the hypha is hardly separated after administration of sanguisorba officinalis charcoal (1 × MIC), so that the growth is obviously inhibited. Then the sanguisorba officinalis charcoal gel with the content of 0.5 percent is administrated to patients for clinical observation, and the clinical curative effect shows that the cure rate of the patients reaches 96.15 percent after the sanguisorba officinalis charcoal gel is used for treating for 4 weeks, wherein half of the patients are cured.

Drawings

The invention will be further explained with reference to the drawings, in which:

FIG. 1 is a figure of charred sanguisorba officinalis prepared by processing sanguisorba officinalis

FIG. 2 shows total ion flow, A-anion mode and B-cation mode of radix Sangusorbae charcoal extract

FIG. 3 the relative content distribution of the components in the extract of Garden Burnet root charcoal

FIG. 4 microscopic observation results of administration of carbonized sanguisorba root to Trichophyton rubrum, wherein A: normal growth control wells; b: after administration of sanguisorba officinalis charcoal (0.5 × MIC); c: after administration of Garden Burnet root charcoal (1 × MIC)

Detailed Description

The technical solution of the present invention is further described below with reference to specific examples, which are not intended to limit the technical solution.