阅读说明：本技术 一种提高作物耐盐性的调控剂及其制备方法 (Regulating agent for improving salt tolerance of crops and preparation method thereof ) 是由 孟维伟 刘灵艳 南镇武 王旭清 赵雪峰 刘开昌 赵增祥 尹训彬 张萍 徐杰 王娜 于 2020-05-29 设计创作，主要内容包括：本发明属于农作物种植技术领域,具体涉及一种提高作物耐盐性的调控剂,并进一步公开其制备方法。本发明所述提高作物耐盐性能的调控剂以木贼的乙醇提取物和/或瓦松的水提取物为有效成分,仅通过简单的浸渍处理即可有效促进玉米种子的耐盐性能及在盐碱环境下的萌发性能,对于盐碱环境下玉米作物的种植具有重要的农业意义。(The invention belongs to the technical field of crop planting, and particularly relates to a regulating agent for improving the salt tolerance of crops, and further discloses a preparation method of the regulating agent. The regulating agent for improving the salt tolerance of the crops takes the ethanol extract of the equisetum hiemale and/or the water extract of the orostachys fimbriatus as the effective components, can effectively promote the salt tolerance of the corn seeds and the germination performance in the saline-alkali environment only through simple dipping treatment, and has important agricultural significance for the planting of the corn crops in the saline-alkali environment.)

1. A regulating agent for improving the salt tolerance of crops is characterized in that the regulating agent is an aqueous solution containing an equisetum extract and/or a herba orostachyos extract.

2. The regulator for improving the salt tolerance of crops as claimed in claim 1, wherein the mass concentration of the equisetum hiemale extract and/or the orostachys fimbriatus extract is controlled to be 1-5 g/L.

3. The regulator for improving the salt tolerance of crops as claimed in claim 1 or 2, wherein the equisetum hiemale extract is ethanol extract of equisetum hiemale.

4. The modulator for improving the salt tolerance of crops as claimed in claim 1 or 2, wherein the extract of orostachys fimbriatus is an aqueous extract of orostachys fimbriatus.

5. The modulator for improving salt tolerance of crops as claimed in any one of claims 1 to 4, wherein the modulator further comprises inositol and Mn²⁺、Ca²⁺At least one of (1).

6. A method for preparing the regulator for improving the salt tolerance of crops as claimed in any one of claims 1 to 5, which comprises the steps of uniformly mixing selected amounts of the equisetum hiemale extract and the orostachys fimbriatus extract and adding water for dispersion.

7. A control method for improving salt tolerance of crops, which comprises the step of soaking seeds of the crops in the control agent of any one of claims 1 to 5 at normal temperature.

8. The method as claimed in claim 7, wherein the ratio of the seeds of the plant to the controlling agent is controlled to be 100-300 g/L.

9. A method as claimed in claim 7 or 8, wherein the seed soaking time is controlled to be 2-5 h.

10. The method as claimed in any one of claims 7 to 9, wherein the crop comprises maize.

Technical Field

The invention belongs to the technical field of crop planting, and particularly relates to a regulating agent for improving the salt tolerance of crops, and further discloses a preparation method of the regulating agent.

Background

Soil salinization is one of the most main abiotic stresses harming agricultural production at present and becomes an important environmental factor restricting agricultural development in China. According to statistics, about 0.2 hundred million hm is found in China²The saline-alkali soil accounts for about 20% of the cultivated land area, and the secondary salinization of the soil is increasingly serious along with the aggravation of industrial pollution, the enlargement of irrigation and soil protection areas, improper use of chemical fertilizers and the like.

Studies have shown that the effects of salt stress on seed germination are several: inhibiting seed germination without loss of seed vigor; delaying but not inhibiting seed germination; the seeds are permanently devitalized. The most common and significant influence of salt on non-halophytes is growth inhibition, and high-concentration salt in saline soil has certain influence on plants, mainly expressed in the aspects of unbalanced osmotic stress, ion toxicity and ion absorption, and generally can reduce seed germination rate, delay seedling growth, inhibit photosynthesis and further reduce crop yield. The existing research results also show that the salt stress obviously inhibits the growth of the seed buds and roots.

Therefore, how to effectively improve the salt tolerance of the crop seeds has positive significance for improving the planting efficiency of the crops in the saline-alkali environment.

Disclosure of Invention

Therefore, the technical problem to be solved by the invention is to provide a regulating agent for improving the salt tolerance of crops, so that the germination efficiency of crop seeds in a saline-alkali environment is effectively improved;

the second technical problem to be solved by the present invention is to provide a method for preparing the above-mentioned modulating agent.

In order to solve the technical problems, the invention provides a regulating agent for improving the salt tolerance of crops, wherein the regulating agent is an aqueous solution containing an equisetum hiemale extract and/or an orostachys fimbriatus extract.

Specifically, the mass concentration of the equisetum hiemale extract and/or the orostachys fimbriatus extract is controlled to be 1-5 g/L.

Specifically, the equisetum hiemale extract is an ethanol extract of equisetum hiemale.

Specifically, the method for extracting the equisetum hiemale extract comprises the following steps:

(1) crushing the traditional Chinese medicine material horsetail after impurity removal and air drying;

(2) leaching the crushed equisetum hiemale with 50% ethanol water;

(3) collecting the leaching solution, and concentrating the leaching solution until the amount of the leaching solution is the same as that of the horsetail to obtain the required horsetail extract.

Specifically, the herba orostachyos extract is a water extract of herba orostachyos.

Specifically, the extraction method of the herba orostachyos extract comprises the following steps:

(1) cleaning whole plant of Orostachys japonicas, air drying, and pulverizing;

(2) decocting pulverized herba Orostachyos in water;

(3) collecting decoction, and concentrating until the dosage of the decoction is the same as that of the orostachys fimbriatus to obtain the desired orostachys fimbriatus extract.

Preferably, the mass ratio of the equisetum hiemale extract to the orostachys fimbriatus extract is controlled to be 1: 1-3.

More preferably, the regulator further comprises inositol and Mn²⁺、Ca²⁺At least one of; in particular, the method comprises the following steps of,

the addition concentration of the inositol is 0.2-0.5 g/L;

the Mn is²⁺Is added at a concentration of 0.3-0.8 g/L, and MnCl is added₂Preferably;

the Ca²⁺Is added at a concentration of 0.3-0.8 g/L, and CaCl is added₂It is preferable.

The invention also discloses a method for preparing the regulating agent for improving the salt tolerance of crops, which comprises the steps of uniformly mixing a selected amount of the equisetum hiemale extract and the orostachys fimbriatus extract and adding water for dispersing.

The invention also discloses a regulation and control method for improving the salt tolerance of crops, which comprises the step of placing seeds of the crops in the regulation and control agent and carrying out seed soaking treatment at normal temperature.

Specifically, the material-liquid ratio of the seeds of the crops to the regulating agent is controlled to be 100-300 g/L.

Specifically, the seed soaking time is controlled to be 2-5 h.

In particular, the crop comprises corn.

The regulating agent for improving the salt tolerance of the crops takes the ethanol extract of equisetum hiemale and/or the water extract of orostachys fimbriatus as the effective components, in the prior art, the ethanol extract of equisetum hiemale is used for improving the cold tolerance foliar fertilizer of wheat, and the water extract of orostachys fimbriatus is used for improving the effective components of the coating agent of the cold tolerance of wheat, the applicant finds that the ethanol extract of equisetum hiemale and the water extract of orostachys fimbriatus have certain promotion effects on improving the salt tolerance of corn seeds, can effectively promote the salt tolerance of the corn seeds and the germination performance in the saline-alkali environment only through simple dipping treatment, and has important agricultural significance on the planting of the corn crops in the saline-alkali environment.

Detailed Description

Preparation example 1

Removing impurities from a traditional Chinese medicine herba Equiseti hiemalis, air drying, crushing to 40-60 meshes, adding 6 times of 50% ethanol water solution into the herba Equiseti hiemalis, and leaching at 60 ℃ for 1 hour; filtering to obtain extractive solution, extracting the residue with the same method for 2 times, mixing the extractive solutions for 3 times, recovering ethanol at 70 deg.C, and evaporating to concentrate until the extract amount is the same as the amount of herba Equiseti hiemalis to obtain the desired ethanol extract of herba Equiseti hiemalis.

Preparation example 2

Cleaning whole herba Orostachyos, air drying, pulverizing, adding water 5 times of the weight of herba Orostachyos pulverized particles, soaking for 30min, and heating for decocting for 30 min; and (3) after solid-liquid separation, collecting decoction, continuously adding the same amount of water into the collected residues for secondary decoction extraction, collecting the decoction, combining the decoction with the previous decoction, concentrating the mixture by rotary evaporation to obtain an extract, and stopping concentration when the mass of the extract is the same as the using amount of the orostachys fimbriatus so as to obtain the required orostachys fimbriatus extract.